RESUMO
The organization of enzymes into different subcellular compartments is essential for correct cell function. Protein-based cages are a relatively recently discovered subclass of structurally dynamic cellular compartments that can be mimicked in the laboratory to encapsulate enzymes. These synthetic structures can then be used to improve our understanding of natural protein-based cages, or as nanoreactors in industrial catalysis, metabolic engineering, and medicine. Since the function of natural protein-based cages is related to their three-dimensional structure, it is important to determine this at the highest possible resolution if viable nanoreactors are to be engineered. Cryo-electron microscopy (cryo-EM) is ideal for undertaking such analyses within a feasible time frame and at near-native conditions. This review describes how three-dimensional cryo-EM is used in this field and discusses its advantages. An overview is also given of the nanoreactors produced so far, their structure, function, and applications.
Assuntos
Microscopia Crioeletrônica , Enzimas Imobilizadas , Engenharia Metabólica , Nanotecnologia , Microscopia Crioeletrônica/instrumentação , Microscopia Crioeletrônica/métodos , Enzimas Imobilizadas/química , Enzimas Imobilizadas/ultraestrutura , Humanos , Engenharia Metabólica/instrumentação , Engenharia Metabólica/métodos , Engenharia Metabólica/tendências , Nanotecnologia/instrumentação , Nanotecnologia/métodos , Nanotecnologia/tendências , Retratos como AssuntoRESUMO
A big hurdle for the use of protein-based drugs is that they are easily degraded by proteases in the human body. In an attempt to solve this problem, we show the possibility to functionalize TM encapsulin nanoparticles with an mEETI-II knottin miniprotein from the cysteine-stabilized knot class. The resulting particles did not show aggregation and retained part of their protease inhibitive function. This imposes a protection toward protease, in this case, trypsin, degradation of the protein cage. The used chemistry is easy to apply and thus suitable to protect other protein systems from degradation. In addition, this proof of principle opens up the use of other knottins or cysteine-stabilized knots, which can be attached to protein cages to create a heterofunctionalized protein nanocage. This allows specific targeting and tumor suppression among other types of functionalization. Overall, this is a promising strategy to protect a protein of interest which brings oral administration of protein-based drugs one step closer.
Assuntos
Miniproteínas Nó de Cistina/química , Portadores de Fármacos/química , Inibidores da Tripsina/química , Tripsina/metabolismo , Administração Oral , Proteínas de Bactérias/administração & dosagem , Proteínas de Bactérias/metabolismo , Miniproteínas Nó de Cistina/farmacologia , Portadores de Fármacos/farmacologia , Nanopartículas/química , Proteólise , Proteínas Recombinantes/administração & dosagem , Proteínas Recombinantes/metabolismo , Thermotoga maritima , Inibidores da Tripsina/farmacologiaRESUMO
Viral protein cage-based nanoreactors can be generated by encapsulation of catalytic metal nanoparticles within the capsid structure. In this method, coat proteins of the cowpea chlorotic mottle virus (CCMV) are used to sequester gold nanoparticles (Au NPs) in buffered solutions at neutral pH to form CCMV-Au hybrid nanoparticles. This chapter describes detailed methods for the encapsulation of Au NPs into CCMV protein cages. Protocols for the reduction of nitroarenes by using CCMV-Au NPs as catalyst are described as an example for the catalytic activity of Au NPs in the protein cages.
Assuntos
Bromovirus , Proteínas do Capsídeo , Ouro , Nanopartículas Metálicas , Proteínas do Capsídeo/química , Catálise , Ouro/química , Ligantes , Nanopartículas Metálicas/química , Análise EspectralRESUMO
Protein-based nanoreactors are generated by encapsulating an enzyme inside the capsid of the cowpea chlorotic mottle virus (CCMV). Here, three different noncovalent methods are described to efficiently incorporate enzymes inside the capsid of these viral protein cages. The methods are based on pH, leucine zippers, and electrostatic interactions respectively, as a driving force for encapsulation. The methods are exclusively described for the enzymes horseradish peroxidase, glucose oxidase, and Pseudozyma antarctica lipase B, but they are also applicable for other enzymes.
Assuntos
Reatores Biológicos/virologia , Bromovirus/metabolismo , Enzimas/metabolismo , Nanopartículas/metabolismo , Capsídeo/metabolismo , Proteínas do Capsídeo/metabolismo , Concentração de Íons de Hidrogênio , Eletricidade Estática , Proteínas Virais/metabolismoRESUMO
Insight into the assembly and disassembly of viruses can play a crucial role in developing cures for viral diseases. Specialized fluorescent probes can benefit the study of interactions within viruses, especially during cell studies. In this work, we developed a strategy based on Förster resonance energy transfer (FRET) to study the assembly of viruses without labeling the exterior of viruses. Instead, we exploit their encapsulation of nucleic cargo, using three different fluorescent ATTO dyes linked to single-stranded DNA oligomers, which are hybridised to a longer DNA strand. FRET is induced upon assembly of the cowpea chlorotic mottle virus, which forms monodisperse icosahedral particles of about 22 nm, thereby increasing the FRET efficiency by a factor of 8. Additionally, encapsulation of the dyes in virus-like particles induces a two-step FRET. When the formed constructs are disassembled, this FRET signal is fully reduced to the value before encapsulation. This reversible behavior makes the system a good probe for studying viral assembly and disassembly. It, furthermore, shows that multi-component supramolecular materials are stabilized in the confinement of a protein cage.
Assuntos
Proteínas do Capsídeo/química , DNA/química , Transferência Ressonante de Energia de Fluorescência/métodos , Corantes Fluorescentes/química , Vírus de Plantas/metabolismo , DNA/metabolismoRESUMO
Understanding the assembly pathway of viruses can contribute to creating monodisperse virus-based materials. In this study, the cowpea chlorotic mottle virus (CCMV) is used to determine the interactions between the capsid proteins of viruses and their cargo. The assembly of the capsid proteins in the presence of different lengths of short, single-stranded (ss) DNA is studied at neutral pH, at which the protein-protein interactions are weak. Chromatography, electrophoresis, microscopy, and light scattering data show that the assembly efficiency and speed of the particles increase with increasing length of oligonucleotides. The minimal length required for assembly under the conditions used herein is 14 nucleotides. Assembly of particles containing such short strands of ssDNA can take almost a month. This slow assembly process enabled the study of intermediate states, which confirmed a low cooperative assembly for CCMV and allowed for further expansion of current assembly theories.
Assuntos
Bromovirus/química , Bromovirus/metabolismo , Proteínas do Capsídeo/química , Oligonucleotídeos/química , Oligonucleotídeos/metabolismo , Proteínas do Capsídeo/metabolismo , Concentração de Íons de HidrogênioRESUMO
Introduction of compartments with defined spaces inside a hydrogel network brings unique features, such as cargo quantification, stabilization and diminishment of burst release, which are all desired for biomedical applications. As a proof of concept, guest-modified cowpea chlorotic mottle virus (CCMV) particles and complementary guest-modified hydroxylpropyl cellulose (HPC) were non-covalently cross-linked through the formation of ternary host-guest complexes with cucurbit[8]uril (CB[8]). Furthermore, CCMV based virus-like particles (VLPs) loaded with tetrasulfonated zinc phthalocyanine (ZnPc) were prepared, with a loading efficiency up to 99%, which are subsequently successfully integrated inside the supramolecular hydrogel network. It was shown that compartments provided by protein cages not only help to quantify the loaded ZnPc cargo, but also improve the water solubility of ZnPc to avoid undesired aggregation. Moreover, the VLPs together with ZnPc cargo can be released in a controlled way without an initial burst release. The photodynamic effect of ZnPc molecules was retained after encapsulation of capsid protein and release from the hydrogel. This line of research suggests a new approach for sophisticated drug administration in supramolecular hydrogels.
RESUMO
The packaging of proteins into discrete compartments is an essential feature for cellular efficiency. Inspired by Nature, we harness virus-like assemblies as artificial nanocompartments for enzyme-catalyzed cascade reactions. Using the negative charges of nucleic acid tags, we develop a versatile strategy to promote an efficient noncovalent co-encapsulation of enzymes within a single protein cage of cowpea chlorotic mottle virus (CCMV) at neutral pH. The encapsulation results in stable 21-22 nm sized CCMV-like particles, which is characteristic of an icosahedral T = 1 symmetry. Cryo-EM reconstruction was used to demonstrate the structure of T = 1 assemblies templated by biological soft materials as well as the extra-swelling capacity of these T = 1 capsids. Furthermore, the specific sequence of the DNA tag is capable of operating as a secondary biocatalyst as well as bridging two enzymes for co-encapsulation in a single capsid while maintaining their enzymatic activity. Using CCMV-like particles to mimic nanocompartments can provide valuable insight on the role of biological compartments in enhancing metabolic efficiency.
Assuntos
Bromovirus/enzimologia , Glucose Oxidase/metabolismo , Ácidos Nucleicos/metabolismo , Fosfogluconato Desidrogenase/metabolismo , Fosfotransferases (Aceptor do Grupo Álcool)/metabolismo , Biocatálise , Bromovirus/química , Bromovirus/metabolismo , Glucose Oxidase/química , Ácidos Nucleicos/química , Tamanho da Partícula , Fosfogluconato Desidrogenase/química , Fosfotransferases (Aceptor do Grupo Álcool)/química , Propriedades de SuperfícieRESUMO
Abundant and highly diverse, viruses offer new scaffolds in nanotechnology for the encapsulation, organization, or even synthesis of novel materials. In this work the coat protein of the cowpea chlorotic mottle virus (CCMV) is used to encapsulate gold nanoparticles with different sizes and stabilizing ligands yielding stable particles in buffered solutions at neutral pH. The sizes of the virus-like particles correspond to T = 1, 2, and 3 Caspar-Klug icosahedral triangulation numbers. We developed a simple one-step process enabling the encapsulation of commercially available gold nanoparticles without prior modification with up to 97% efficiency. The encapsulation efficiency is further increased using bis-p-(sufonatophenyl)phenyl phosphine surfactants up to 99%. Our work provides a simplified procedure for the preparation of metallic particles stabilized in CCMV protein cages. The presented results are expected to enable the preparation of a variety of similar virus-based colloids for current focus areas.