Real-Time Wireless Sensing of Cardiomyocyte Contractility by Integrating Magnetic Microbeam and Oriented Nanofibers.

In vitro cardiomyocyte mechano-sensing platform is crucial for evaluating the mechanical performance of cardiac tissues and will be an indispensable tool for application in drug discovery and disease mechanism study. Magnetic sensing offers significant advantages in real-time, in situ wireless monitoring and resistance to ion interference. However, due to the mismatch between the stiffness of traditional rigid magnetic material and myocardial tissue, sensitivity is insufficient and it is difficult to achieve cell structure induction and three-dimensional cultivation. Herein, a magnetic sensing platform that integrates a neodymium-iron-boron/polydimethylsiloxane (NdFeB/PDMS) flexible microbeam with suspended and ordered polycaprolactone (PCL) nanofiber membranes was developed, providing a three-dimensional anisotropic culture environment for cardiomyocyte growth and simultaneously realizing in situ wireless contractility monitoring. The as-prepared sensor presented an ultrahigh sensitivity of 442.2 µV/µm and a deflection resolution of 2 µm. By continuously monitoring the cardiomyocyte growth status and drug stimulation feedback, we verified the capability of the platform to capture dynamic changes in cardiomyocyte contractility. This platform provides a perspective tool for evaluating cardiomyocyte maturity and drug performance.

In-situ and label-free measurement of cytochrome C concentration with a Ti2C-MXene sensitized fiber-optic MZI sensor.

BACKGROUND: The concentration of cytochrome C is demonstrated to be an effective indicator of the microbial corrosion strength of metals. Traditional cytochrome C sensor can detect cytochrome C with a low detection limit, but their use is limited by their high cost, cumbersome operation, and susceptibility to malignant environments. In addition, studies on the monitoring of cytochrome C in the field of microbial corrosion has still not been carried out. Therefore, there is a need for a highly sensitive, selective, low-cost, anti-interference, and stable cytochrome C sensor with online monitoring and remote sensing capabilities for in-situ measurement of microbial corrosion strength. RESULTS: This paper proposed a highly sensitive label-free fiber-optic sensor based on Mach-Zehnder interferometer (MZI) for in-situ measurement of the microbial corrosion marker cytochrome C. Two-dimensional Ti2C-MXene material is uniformly immobilized onto the surface of the sensing area to improve the sensitivity, hydrophilicity, and specific surface area of the sensing area, as well as to facilitate the immobilization of specific sensitive materials. The cytochrome C antibody is modified on the surface of Ti2C-MXene to specifically recognize cytochrome C, whose concentration variation can be measured by monitoring the spectral shift of MZI sensor. Results demonstrate a measurement sensitivity of 1.428 nm/µM for cytochrome C concentrations ranging from 0 to 7.04 µM. The detection limit of the sensor is calculated to be 0.392 µM with remarkable performance, including selectivity, stability, and reliability. Besides, the measurement result of the proposed sensor in real microbial corrosive environment is consistent with that of the ideal environment. SIGNIFICANCE AND NOVELTY: This is the first instance of achieving in-situ and label-free measurement of cytochrome C by using a fiber-optic MZI sensor, which undoubtedly provides a feasible solution for the effective monitoring of microbial metal corrosion in the environment.

A New Planar Potentiometric Sensor for In Situ Measurements.

A new construction of a potentiometric sensor was introduced for the first time. It relies on the use of two membranes instead of one, as in the well-known coated-disc electrode. For this purpose, a new electrode body was constructed, including not one, but two glassy carbon discs covered with an ion-selective membrane. This solution allows for the sensor properties to be enhanced without using additional materials (layers or additives) on the membrane. The new construction is particularly useful for in situ measurements in environmental samples. Two ion-selective polymeric membranes were used, namely H+ and K+-selective membranes, to confirm the universality of the idea. The tests conducted included chronopotentiometric tests, electrochemical impedance spectroscopy, and potentiometric measurements. The electrical and analytical parameters of the sensors were evaluated and compared for all tested electrodes to evaluate the properties of the planar electrode versus previously known constructions. Research has shown that the application of two membranes instead of one allows for the resistance of an electrode to be lowered and for the electrical capacitance to be elevated. Improving the electrical properties of an electrode resulted in the enhancement of its analytical properties. The pH measurement range of the planar electrode is 2-11, which is much wider in contrast to that of the single-membrane electrode. The linear range of the K+-selective planar electrode is wider than that of the coated-disc electrode and equals 10-6 to 10-1 M. The response time turned out to be a few seconds shorter, and the potential drift was smaller due to the application of an additional membrane in the electrode construction. This research creates a new opportunity to design robust potentiometric sensors, as the presented construction is universal and can be used to obtain electrodes selective to various ions.

Operando Nuclear Magnetic Resonance (NMR) Studies of a Trickle-bed Reactor Using D-T2 Correlations.

Catalytic conversions in fine-chemical and pharmaceutical production are increasingly performed in trickle-bed rectors. Optimisation of these processes is usually based on end of pipe measurement made at specific residence times. This process is both time-consuming and the data sometimes challenging to interpret. In the present work, operando nuclear magnetic resonance (NMR) techniques both at the scale of the whole bed (global) and spatially resolved within the bed (local) are used to gain new insights into the catalytic conversion process under reaction conditions. Spatially resolved spectroscopic and diffusion-T2-relaxation (D-T2) methods interrogate local differences in chemical conversion and selectivity, and mass transport (molecular self-diffusion) respectively, thereby providing valuable information for process simulation models. This capability is demonstrated using the continuous flow three phase (gas-liquid-solid) hydrogenation of benzonitrile over a fixed bed of 0.5 wt% Pd/Al2O3 catalyst pellets yielding toluene and benzylamine. Global 1H spectroscopic and D-T2 were used to monitor chemical conversion and the approach to steady state; these were subsequently followed by spatially resolved 1H spectra and spatially resolved D-T2 correlations to examine the local differences in axial conversion and selectivity of the catalyst bed packing. At steady-state a global conversion of 63% was achieved with 65% and 25% selectivity to benzylamine and toluene respectively. Heterogeneities in the local (axial) conversion and selectivity differed by 31% along the total catalyst bed length. These techniques should be applicable to many three-phase heterogeneous catalytic systems provided that the T2 relaxation time of the reactants and products is not prohibitively small.

In Situ Real-Time Observation of Photoinduced Nanoscale Azo-Polymer Motions Using High-Speed Atomic Force Microscopy Combined with an Inverted Optical Microscope.

High-speed atomic force microscopy (HS-AFM) is an indispensable technique in the field of biology owing to its imaging capability with high spatiotemporal resolution. Furthermore, recent developments established tip-scan stand-alone HS-AFM combined with an optical microscope, drastically improving its versatility. It has considerable potential to contribute to not only biology but also various research fields. A great candidate is a photoactive material, such as an azo-polymer, which is important for optical applications because of its unique nanoscale motion under light irradiation. Here, we demonstrate the in situ observation of nanoscale azo-polymer motion by combining tip-scan HS-AFM with an optical system, allowing HS-AFM observations precisely aligned with a focused laser position. We observed the dynamic evolution of unique morphologies in azo-polymer films. Moreover, real-time topographic line profile analyses facilitated precise investigations of the morphological changes. This important demonstration would pave the way for the application of HS-AFM in a wide range of research fields.

In Situ X-ray Scattering Reveals Coarsening Rates of Superlattices Self-Assembled from Electrostatically Stabilized Metal Nanocrystals Depend Nonmonotonically on Driving Force.

Self-assembly of colloidal nanocrystals (NCs) into superlattices (SLs) is an appealing strategy to design hierarchically organized materials with promising functionalities. Mechanistic studies are still needed to uncover the design principles for SL self-assembly, but such studies have been difficult to perform due to the fast time and short length scales of NC systems. To address this challenge, we developed an apparatus to directly measure the evolving phases in situ and in real time of an electrostatically stabilized Au NC solution before, during, and after it is quenched to form SLs using small-angle X-ray scattering. By developing a quantitative model, we fit the time-dependent scattering patterns to obtain the phase diagram of the system and the kinetics of the colloidal and SL phases as a function of varying quench conditions. The extracted phase diagram is consistent with particles whose interactions are short in range relative to their diameter. We find the degree of SL order is primarily determined by fast (subsecond) initial nucleation and growth kinetics, while coarsening at later times depends nonmonotonically on the driving force for self-assembly. We validate these results by direct comparison with simulations and use them to suggest dynamic design principles to optimize the crystallinity within a finite time window. The combination of this measurement methodology, quantitative analysis, and simulation should be generalizable to elucidate and better control the microscopic self-assembly pathways of a wide range of bottom-up assembled systems and architectures.

Future of the Search for Life: Workshop Report.

The 2-week, virtual Future of the Search for Life science and engineering workshop brought together more than 100 scientists, engineers, and technologists in March and April 2022 to provide their expert opinion on the interconnections between life-detection science and technology. Participants identified the advances in measurement and sampling technologies they believed to be necessary to perform in situ searches for life elsewhere in our Solar System, 20 years or more in the future. Among suggested measurements for these searches, those pertaining to three potential indicators of life termed "dynamic disequilibrium," "catalysis," and "informational polymers" were identified as particularly promising avenues for further exploration. For these three indicators, small breakout groups of participants identified measurement needs and knowledge gaps, along with corresponding constraints on sample handling (acquisition and processing) approaches for a variety of environments on Enceladus, Europa, Mars, and Titan. Despite the diversity of these environments, sample processing approaches all tend to be more complex than those that have been implemented on missions or envisioned for mission concepts to date. The approaches considered by workshop breakout groups progress from nondestructive to destructive measurement techniques, and most involve the need for fluid (especially liquid) sample processing. Sample processing needs were identified as technology gaps. These gaps include technology and associated sampling strategies that allow the preservation of the thermal, mechanical, and chemical integrity of the samples upon acquisition; and to optimize the sample information obtained by operating suites of instruments on common samples. Crucially, the interplay between science-driven life-detection strategies and their technological implementation highlights the need for an unprecedented level of payload integration and extensive collaboration between scientists and engineers, starting from concept formulation through mission deployment of life-detection instruments and sample processing systems.

In-situ measurement of dissolved sulfide in surface sediment porewater using diffusive gradients in thin films (DGT) coupled with digital imaging.

Dissolved sulfide in sediment porewater significantly influences aquatic ecosystems. Conventionally, sulfide determination in sediment porewater relies on ex-situ analytical methods, susceptible to measurement errors due to sulfide oxidation and volatilization during sample analysis. In this study, we introduced an innovative in-situ method for assessing dissolved sulfide in surface sediment porewater, leveraging the integration of diffusive gradients in thin films (DGT) with digital imaging. The DGT device effectively concentrates sulfide in sediment porewater, inducing observable color changes in the binding gel. Recordings of these changes, captured by imaging equipment, facilitated the establishment of calibration curves correlating grayscale value alterations in the binding gel to sulfide concentrations. Under optimal conditions, the developed method demonstrated a linear detection range of 3.0-200 µmol L-1 at 20 °C, particularly when the exposure time exceeded 180 min. The developed method is insensitive to salinity and suitable for measuring sulfide concentrations in various natural water environments. Compared to traditional ex-situ methods, our approach circumvents challenges linked to intricate pre-treatment, prolonged analysis duration, and significant systemic errors. This proposed method presents a real-time solution for sulfide concentration assessment in surface sediment porewater, empowering researchers with an efficient means to monitor and study dynamic sulfide levels.

Aligned Conjugated Polymer Nanofiber Networks in an Elastomer Matrix for High-Performance Printed Stretchable Electronics.

Conjugated polymer films are promising in wearable X-ray detection. However, achieving optimal film microstructure possessing good electrical and detection performance under large deformation via scalable printing remains challenging. Herein, we report bar-coated high-performance stretchable films based on a conjugated polymer P(TDPP-Se) and elastomer SEBS blend by optimizing the solution-processing conditions. The moderate preaggregation in solution and prolonged growth dynamics from a solvent mixture with limited dissolving capacity is critical to forming aligned P(TDPP-Se) chains/crystalline nanofibers in the SEBS phase with enhanced π-π stacking for charge transport and stress dissipation. The film shows a large elongation at break of >400% and high mobilities of 5.29 cm2 V-1 s-1 at 0% strain and 1.66 cm2 V-1 s-1 over 500 stretch-release cycles at 50% strain, enabling good X-ray imaging with a high sensitivity of 1501.52 µC Gyair-1 cm-2. Our work provides a morphology control strategy toward high-performance conjugated polymer film-based stretchable electronics.

Measurement of perfluoroalkyl substances in drinking water sources by DGT sampler with a novel fluorinated graphite binding gel.

Extensive use of per- and polyfluoroalkyl substances (PFASs) has resulted in their widespread presence in natural waters. Concern for public health requires reliable measurement methods for determining their distribution and risks. Here, a sampling method based on diffusive gradients in thin films (DGT) was developed for measuring PFASs in drinking water sources. Fluorinated graphite (FG) particles were used to prepare the DGT binding gel for selective enrichment of trace PFASs in an aqueous environment. The FG-DGT method did not show sensitivity to relevant environmental parameters including pH (5.0-9.0), ionic strength (0.001-0.5 M), or DOM concentration (0-30 mg/L). The FG-DGT had enough capacity for deployment of up to four months. Six traditional and emerging PFASs including PFOS, PFOA, PFHpA, PFHxS, PFNA, and 6:2 FTSA at the ng/L level were detected in two major reservoirs serving as public drinking water sources by FG-DGT method coupled with liquid chromatography tandem mass spectrometry (LC-MS/MS). PFOA appeared at the highest observed concentrations in the drinking water sources. The research demonstrates that FG-DGT is an effective and efficient tool for monitoring PFASs in drinking water.

Optical Measurement of the Stoichiometry of Thin-Film Compounds Synthetized From Multilayers: Example of Cu(In,Ga)Se2.

The properties of centimeter-sized thin-film compound semiconductors depend upon the morphology and chemical composition of the multiple submicrometer-thick elemental and alloy precursor layers from which they are synthesized. The challenge is to characterize the individual precursor layers over these length scales during a multistep synthesis without altering or contaminating them. Conventional electron and X-ray-based morphological and compositional techniques are invasive, require preparation, and are thus incompatible with in-line synthesis processes. In a proof-of-concept study, we applied confocal laser scanning microscopy (CLSM) as a noninvasive optical imaging technique, which measures three-dimensional surface profiles with nanoscale resolution, to this challenge. Using an array of microdots containing Cu(In,Ga)Se2 semiconductor layers for solar cells as an example, we performed CLSM correlative studies to quantify morphological and layer thickness changes during four stages of a thin-film compound synthesis. Using simple assumptions, we measured the micrometer-scale spatially resolved chemical composition of stacked precursor layers to predict the final material phases formed and predict relative device performance. The high spatial resolution, coupled with the ability to measure sizeable areas without influencing the synthesis at high speed, makes CLSM an excellent prospect for research and quality control tool for thin films.

Revealing Deformation Mechanisms in Polymer-Grafted Thermoplastic Elastomers via In Situ Small-Angle X-ray Scattering.

The tunable properties of thermoplastic elastomers (TPEs), through polymer chemistry manipulations, enable these technologically critical materials to be employed in a broad range of applications. The need to "dial-in" the mechanical properties and responses of TPEs generally requires the design and synthesis of new macromolecules. In these designs, TPEs with nonlinear macromolecular architectures outperform the mechanical properties of their linear copolymer counterparts, but the differences in the deformation mechanism providing enhanced performance are unknown. Here, in situ small-angle X-ray scattering (SAXS) measurements during uniaxial extension reveal distinct deformation mechanisms between a commercially available linear poly(styrene)-poly(butadiene)-poly(styrene) (SBS) triblock copolymer and the grafted SBS version containing grafted poly(styrene) (PS) chains from the poly(butadiene) (PBD) midblock. The neat SBS (φSBS = 100%) sample deforms congruently with the macroscopic dimensions, with the domain spacing between spheres increasing and decreasing along and transverse to the stretch direction, respectively. At high extensions, end segment pullout from the PS-rich domains is detected, which is indicated by a disordering of SBS. Conversely, the PS-grafted SBS that is 30 vol % SBS and 70% styrene (φSBS = 30%) exhibits a lamellar morphology, and in situ SAXS measurements reveal an unexpected deformation mechanism. During deformation, there are two simultaneous processes: significant lamellar domain rearrangement to preferentially orient the lamellae planes parallel to the stretch direction and crazing. The samples whiten at high strains as expected for crazing, which corresponds with the emergence of features in the 2D SAXS pattern during stretching consistent with fibril-like structures that bridge the voids in crazes. The significant domain rearrangement in the grafted copolymers is attributed to the new junctions formed across multiple PS domains by the grafting of a single chain. The in situ SAXS measurements provide insights into the enhanced mechanical properties of grafted copolymers that arise through improved physical cross-linking that leads to nanostructure domain reorientation for self-reinforcement and craze formation where fibrils help to strengthen the polymer.

Crystallization Control for Ambient Printed FA-Based Lead Triiodide Perovskite Solar Cells.

Upscalable printing of high-performance and stable perovskite solar cells (PSCs) is highly desired for commercialization. However, the efficiencies of printed PSCs lag behind those of their lab-scale spin-coated counterparts owing to the lack of systematic understanding and control over perovskite crystallization dynamics. Here, the controlled crystallization dynamics achieved using an additive 1-butylpyridine tetrafluoroborate (BPyBF4 ) for high-quality ambient printed α-formamidinium lead triiodide (FAPbI3 ) perovskite films are reported. Using in situ grazing-incidence wide-angle X-ray scattering and optical diagnostics, the spontaneous formation of α-FAPbI3 from precursors during printing without the involvement of  Î´-FAPbI3 is demonstrated. The addition of BPyBF4 delays the crystallization onset of α-FAPbI3 , enhances the conversion from sol-gel to perovskite, and reduces stacking defects during printing. Therefore, the altered crystallization results in fewer voids, larger grains, and less trap-induced recombination loss within printed films. The printed PSCs yield high power conversion efficiencies of 23.50% and 21.60% for a 0.09 cm-2 area device and a 5 cm × 5 cm-area module, respectively. Improved device stability is further demonstrated, i.e., approximately 94% of the initial efficiency is retained for over 2400 h under ambient conditions without encapsulation. This study provides an effective crystallization control method for the ambient printing manufacture of large-area high-performance PSCs.

Air Temperature and Relative Humidity Datasets from an Urban Meteorological Network in the City Area of Novi Sad (Serbia).

This data article describes two groups of datasets which capture, firstly - 10-minutes air temperature (Ta) and relative humidity (RH) data from 27 urban and non-urban sites over a period of 3.5 years covering 2014-2018; and secondly - hourly Ta data from 12 urban sites over a period of 2 years covering 2016 and 2017. Both datasets are from urban meteorological network located in the Novi Sad city (Serbia). These datasets have 2 different types of information in the collection: one type provides details about the monitoring sites at which the Ta and RH sensors are placed, while the second type contains Ta and RH data at all sensor locations. In all, the 10-minutes dataset contains about 185,000 instances of Ta and RH data, and the hourly datasets contain 17,544 instances of Ta data. The 10-minutes datasets were not quality controlled, but the hourly Ta data has been cleaned and gap-filled so there are 24 measures at each site for each day. There are multiple potential uses, where this data can be applied. It can provide insights in understanding intra-urban and inter-urban research, urban climate modeling on local or micro scales, heat-related public health investigations and urban environment inquiries. It can also be used in machine learning experiments, for example, to test the accuracy of classification algorithms or to build and validate spatio-temporal machine learning functions, either for classification purposes or for gap filling. These datasets are directly citable through its DOIs and available for download from the Zenodo platform or from the Fair Micromet Portal.

Assessment of Electromagnetic Field Exposure on European Roads: A Comprehensive In Situ Measurement Campaign.

The rapid evolution of wireless communication technologies (such as fifth-generation (5G) cellular networks) in the last years has allowed connecting different objects (from wearable electronics to vehicles) and people through communication networks, and at the same time, has led to widespread deployment of base stations. Along with this growth, questions about the potential adverse effects on human health due to electromagnetic fields (EMFs) from base station antennas have also been raised. In this paper, we focus on the assessment of EMFs in automobiles during short (between cities) and long (between countries) trips on several European roads. Comprehensive measurement campaigns were carried out in several European countries: Austria, Bulgaria, Croatia, Hungary, Italy, Slovenia, and the Republic of Serbia. The results show that the median total electric field is 0.23-0.24 V/m in Bulgaria, Croatia, Hungary, Italy, and the Republic of Serbia. In Austria and Slovenia, the median is 0.28-0.31 V/m. Austria demonstrated the highest value for the total electric field, at 17.4 V/m.

One year of high-precision operational data including measurement uncertainties from a large-scale solar thermal collector array with flat plate collectors, located in Graz, Austria.

This work presents operational data of a large-scale solar thermal collector array. The array belongs to a solar thermal plant located at Fernheizwerk Graz, Austria, which feeds into the local district heating network and is one of the largest Solar District Heating installations in Central Europe. The collector array deploys flat plate collectors with a total gross collector area of 516 m2 (361 kW nominal thermal power). Measurement data was collected in situ within the scientific research project MeQuSo using high-precision measurement equipment and implementing extensive data quality assurance measures. Data compromises one full operational year (2017) in a 1-minute sampling rate with a share of missing data of 8.2%. Several files are provided, including data files and Python scripts for data processing and plot generation. The main dataset contains the measured values of various sensors, including volume flow, inlet and outlet temperature of the collector array, outlet temperatures of single collector rows, global tilted and global horizontal irradiance, direct normal irradiance, and weather data (ambient air temperature, wind speed, ambient relative humidity) at the plant location. Beyond the measurement data, the dataset includes additional calculated data channels, such as thermal power output, mass flow, fluid properties, solar incidence angle and shadowing masks. The dataset also provides uncertainty information in terms of standard deviation of a normal distribution, based either on sensor specifications or on error propagation of the sensor uncertainties. Uncertainty information is provided for all continuous variables, with some exceptions such as the solar geometry, where uncertainty is negligible. The data files include a JSON file containing metadata (e.g., plant parameters, data channel descriptions, physical units, etc.) in both human and machine-readable format. The dataset is suitable for detailed performance and quality analysis and for modelling of flat plate collector arrays. Specifically, it can be helpful to improve and validate dynamic collector array models, radiation decomposition and transposition algorithms, short-term thermal power forecasting algorithms with machine learning techniques, performance indicators, in situ performance checks, dynamic optimization procedures such as parameter estimation or MPC control, uncertainty analyses of measurement setups, as well as testing and validation of open-source software code. The dataset is released under a CC BY-SA 4.0 license. To the best knowledge of the authors, there is no comparable dataset of a large-scale solar thermal collector array publicly available.

In Situ Measurement and Reconstruction Technology of Cylindrical Shape of High-Precision Mandrel.

The technology of in situ measurement of cylindrical shapes is an important means of improving the surface machining accuracy of cylindrical workpieces. As a method of cylindricity measurement, the principle of the three-point method has not been fully studied and applied, so it is seldom used in the field of high-precision cylindrical topography measurement. Since the three-point method has the advantages of a simpler measurement structure and smaller system error compared with other multi-point methods, the research on it is still of great significance. Based on the existing research results of the three-point method, this paper proposes the in situ measurement and reconstruction technology of the cylindrical shape of a high-precision mandrel by means of a three-point method. The principle of the technology is deduced in detail and an in situ measurement and reconstruction system is built to carry out the experiments. Experiment results are verified using a commercial roundness meter and the deviation of cylindricity measurement results is 10 nm, which is 2.56% of the measurement results of commercial roundness meters. This paper also discusses the advantages and application prospects of the proposed technology.

In Situ Observations of Halogenated Gases at the Shangdianzi Background Station and Emission Estimates for Northern China.

Halogenated gases include ozone-depleting substances and greenhouse gases, such as chlorofluorocarbons, halons, hydrochlorofluorocarbons, hydrofluorocarbons, and perfluorinated gases. In situ atmospheric observations of major halogenated gases were conducted at the Shangdianzi (SDZ) background station, China, from October 2020 to September 2021 using ODS5-pro, a newly developed measurement system. The measurement time series of 36 halogenated gases showed occasional pollution events, where background conditions represented 25% (CH2Cl2) to 81% (CF3Cl, CFC-13) of the measurements. The annual mean background mole fractions of most species at SDZ were consistent with those obtained at the Mace Head station in Ireland. The background conditions were distinguished from pollution events, and the enhanced mole fractions were used to estimate the emissions of four categories of fluorinated gases (F-gases) from northern China using a tracer ratio method. The CO2-equivalent (CO2-equiv) emission of F-gases from northern China reached 181 ± 18 Tg year-1 during 2020-2021. Among the four categories of F-gases estimated, SF6 accounted for the highest proportion of CO2-equiv emissions (24%), followed by HFC-23 (22%), HFC-125 (17%), HFC-134a (13%), NF3 (10%), CF4 (5.9%), HFC-143a (3.9%), HFC-32 (3.4%), and HFC-152a (0.2%).

In Situ Probing the Mass Transport Property Inside an Imitated Three-Dimensional Porous Bioelectrode.

Three-dimensional porous materials have been demonstrated as the most successful bioelectrodes in bioelectrochemical systems due to their high specific surface area and abundant adhesion regions for electroactive bacteria. However, the pore clogging potentially limits the mass transfer process inside the electrode due to the unreasonable structure design and long-term operation. The investigation of mass transport behavior in the porous scaffolds is of great significance for designing the electrode structure and optimizing bioelectrochemical system performance. To in situ characterize the mass transport behavior in the orderly pore structure, model electrodes with 100 copper wires (10 × 10) are constructed to imitate a three-dimensional porous structure (pore size: ∼150 µm) commonly employed in bioelectrodes. The poor proton effective diffusion coefficient solidly demonstrates that the mass transport inside the three-dimensional porous electrode is critically inhibited, leading not only to a progressive change and sparse biomass in the biofilm development process but also to biofilm acidification due to serious proton accumulation. It finally results in sluggish bacterial metabolic activity and a decreased electrocatalytic capacity. The interior space of porous electrodes cannot be adequately utilized, resulting in the inability to fully exploit the advantages of their abundant surface area. Consequently, the construction of gradient porous electrodes with small inner and large outer pores to enhance mass transport is a feasible proposal for enhancing performance. The proposed methodology of establishing model electrodes combined with the in situ detection technique within porous electrodes is crucial for acquiring various types of physicochemical information inside the bioelectrode, such as biofilm growth situation, biochemical reaction conditions, as well as mass transfer characteristics. More importantly, the work provides a fundamental basis for designing highly efficient bioelectrodes.

IBIL Measurement and Optical Simulation of the DI Center in 4H-SiC.

In this paper, DI defects are studied via experiments and calculations. The 2 MeV H+ is used to carry on an ion-beam-induced luminescence (IBIL) experiment to measure the in-situ luminescence of untreated and annealed 4H-SiC at 100 K. The results show that the luminescence intensity decreases rapidly with increasing H+ fluence, which means the losses of optical defect centers. In addition, the evident peak at 597 nm (2.07 eV) is the characteristic peak of 4H-SiC, and the weak peak between 400 nm and 450 nm is attributed to the DI optical center. Moreover, the first-principles calculation of 4H-SiC is adopted to discuss the origin of DI defects. The optical transition of the defect SiC(CSi)2 from q = 0 to q = 1 is considered the experimental value of the DI defect center.