RESUMO
Protein aggregation in the outermost layers of the cornea, which can lead to cloudy vision and in severe cases blindness, is linked to mutations in the extracellular matrix protein transforming growth factor-ß-induced protein (TGFBIp). Among the most frequent pathogenic mutations are R124H and R555W, both associated with granular corneal dystrophy (GCD) characterized by the early-onset formation of amorphous aggregates. The molecular mechanisms of protein aggregation in GCD are largely unknown. In this study, we determined the crystal structures of R124H, R555W, and the lattice corneal dystrophy-associated A546T. Although there were no changes in the monomeric TGFBIp structure of any mutant that would explain their propensity to aggregate, R124H and R555W demonstrated a new dimer interface in the crystal packing, which is not present in wildtype TGFBIp or A546T. This interface, as seen in both the R124H and R555W structures, involves residue 124 of the first TGFBIp molecule and 555 in the second. The interface is not permitted by the Arg124 and Arg555 residues of wildtype TGFBIp and may play a central role in the aggregation exhibited by R124H and R555W in vivo. Using cross-linking mass spectrometry and in-line size exclusion chromatography-small-angle X-ray scattering, we characterized a dimer formed by wildtype and mutant TGFBIps in solution. Dimerization in solution also involves interactions between the N- and C-terminal domains of two TGFBIp molecules but was not identical to the crystal packing dimerization. TGFBIp-targeted interventions that disrupt the R124H/R555W crystal packing dimer interface might offer new therapeutic opportunities to treat patients with GCD.
Assuntos
Córnea/ultraestrutura , Distrofias Hereditárias da Córnea/genética , Proteínas da Matriz Extracelular/genética , Agregados Proteicos/genética , Fator de Crescimento Transformador beta/genética , Amiloide/genética , Amiloide/ultraestrutura , Córnea/metabolismo , Distrofias Hereditárias da Córnea/patologia , Cristalografia por Raios X , Proteínas da Matriz Extracelular/ultraestrutura , Humanos , Mutação de Sentido Incorreto/genética , Multimerização Proteica/genéticaRESUMO
Distinct mutations in the secreted extracellular matrix protein, fibulin-3 (F3), have been associated with a number of ocular diseases ranging from primary open angle glaucoma to cuticular age-related macular degeneration to a rare macular dystrophy, Malattia Leventinese (ML). The R345W F3 mutation that causes ML leads to F3 misfolding, inefficient secretion and accumulation at higher intracellular steady state levels in cultured cells. Herein, we determined whether fifteen other clinically-identified F3 mutations also led to similar levels of misfolding and secretion defects, which might provide insight into their potential pathogenicity. Surprisingly, we found that only a single F3 variant, L451F, presented with a significant secretion defect (69.5 ± 2.4% of wild-type (WT) F3 levels) and a corresponding increase in intracellular levels (226.8 ± 25.4% of WT F3 levels). Upon follow-up studies, when this conserved residue (L451) was mutated to a charged (Asp or Arg) or bulky (Pro, Trp, Tyr) residue, F3 secretion was also compromised, indicating the importance of small side chains (Leu, Ala, or Gly) at this residue. To uncover potential inherent F3 instability not easily observed under typical culture conditions, we genetically eliminated the sole stabilizing N-linked glycosylation site (N249) from select clinically-identified F3 mutants. This removal exacerbated R345W and L451F secretion defects (19.8 ± 3.0% and 12.4 ± 1.2% of WT F3 levels, respectively), but also revealed a previously undiscovered secretion defect in another C-terminal variant, Y397H (42.0 ± 10.1% of WT F3 levels). Yet, glycan removal did not change the relative secretion of the N-terminal mutants tested (D49A, R140W, I220F). These results highlight the uniqueness and molecular similarities between the R345W and L451F variants and also suggest that previously identified disease-associated mutations (e.g., R140W) are indistinguishable from WT with respect to secretion, hinting that they may lead to disease by an alternative mechanism.
Assuntos
Proteínas da Matriz Extracelular/genética , Glaucoma de Ângulo Aberto/genética , Degeneração Macular/genética , Linhagem Celular , Proteínas da Matriz Extracelular/ultraestrutura , Glaucoma de Ângulo Aberto/patologia , Humanos , Degeneração Macular/patologia , Mutação/genética , Drusas do Disco Óptico/congênito , Drusas do Disco Óptico/genética , Drusas do Disco Óptico/patologia , Dobramento de Proteína , Estabilidade Proteica , Retina/metabolismo , Retina/patologiaRESUMO
The lens, suspended in the middle of the eye by tendon-like ciliary zonule fibers and facing three different compartments of the eye, is enclosed in what has been described as the thickest basement membrane in the body. While the protein components of the capsule have been a subject of study for many years, the dynamics of capsule formation, and the region-specific relationship of its basement membrane components to one another as well as to other matrix molecules remains to be explored. Through high resolution confocal and super-resolution imaging of the lens capsule and 3D surface renderings of acquired z-stacks, our studies revealed that each of its basement membrane proteins, laminin, collagen IV, nidogen and perlecan, has unique structure, organization, and distribution specific both to the region of the lens that the capsule is located in and the position of the capsule within the eye. We provide evidence of basal membrane gradients across the depth of the capsule as well as the synthesis of distinct basement membrane lamella within the capsule. These distinctions are most prominent in the equatorial capsule zone where collagen IV and nidogen span the capsule depth, while laminin and perlecan are located in two separate lamellae located at the innermost and outermost capsule domains. We discovered that an extracapsular matrix compartment rich in the connective tissue-like matrix molecules fibronectin, tenascin-C, and fibrillin is integrated with the superficial surface of the lens capsule. Each matrix protein in this extracapsular zone also exhibits region-specific distribution with fibrils of fibrillin, the matrix protein that forms the backbone of the ciliary zonules, inserting within the laminin/perlecan lamella at the surface of the equatorial lens capsule.
Assuntos
Membrana Basal/metabolismo , Tecido Conjuntivo/metabolismo , Proteínas da Matriz Extracelular/ultraestrutura , Cristalino/fisiologia , Animais , Embrião de Galinha , Colágeno Tipo I/metabolismo , Colágeno Tipo I/ultraestrutura , Tecido Conjuntivo/ultraestrutura , Matriz Extracelular/metabolismo , Matriz Extracelular/ultraestrutura , Proteínas da Matriz Extracelular/metabolismo , Fibrilinas/metabolismo , Fibrilinas/ultraestrutura , Fibronectinas/metabolismo , Fibronectinas/ultraestrutura , Proteoglicanas de Heparan Sulfato/química , Proteoglicanas de Heparan Sulfato/metabolismo , Laminina/metabolismo , Laminina/ultraestrutura , Glicoproteínas de Membrana/metabolismo , Glicoproteínas de Membrana/ultraestrutura , Camundongos , Microscopia Confocal , Tenascina/química , Tenascina/metabolismoRESUMO
Albumin-based hydrogels are increasingly attractive in tissue engineering because they provide a xeno-free, biocompatible and potentially patient-specific platform for tissue engineering and drug delivery. The majority of research on albumin hydrogels has focused on bovine serum albumin (BSA), leaving human serum albumin (HSA) comparatively understudied. Different gelation methods are usually employed for HSA and BSA, and variations in the amino acid sequences of HSA and BSA exist; these account for differences in the hydrogel properties. Heat-induced gelation of aqueous HSA is the easiest method of synthesizing HSA hydrogels however hydrogel opacity and poor cell attachment limit their usefulness in downstream applications. Here, a solution to this problem is presented. Stable and translucent HSA hydrogels were created by controlled thermal gelation and the addition of sodium chloride. The resulting bio-inert hydrogel was then subjected to air plasma treatment which functionalised its surface, enabling the attachment of basement membrane matrix (Geltrex). In vitro survival and proliferation studies of foetal human osteoblasts subsequently demonstrated good biocompatibility of functionalised albumin hydrogels compared to untreated samples. Thus, air plasma treatment enables functionalisation of inert heat-derived HSA hydrogels with extracellular matrix proteins and these may be used as a xeno-free platform for biomedical research or cell therapy.
Assuntos
Materiais Biocompatíveis/química , Hidrogéis/química , Gases em Plasma , Albumina Sérica Humana/química , Engenharia Tecidual/métodos , Materiais Biocompatíveis/toxicidade , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Proteínas da Matriz Extracelular/química , Proteínas da Matriz Extracelular/toxicidade , Proteínas da Matriz Extracelular/ultraestrutura , Temperatura Alta , Humanos , Hidrogéis/toxicidade , Teste de Materiais , Microscopia Eletrônica de Varredura , Osteoblastos , Albumina Sérica Humana/toxicidade , Albumina Sérica Humana/ultraestrutura , Cloreto de Sódio/química , Propriedades de SuperfícieRESUMO
Biomineralization by living organisms are common phenomena observed everywhere. Molluskan shells are representative biominerals that have fine microstructures with controlled morphology, polymorph, and orientation of CaCO3 crystals. A few organic molecules involved in the biominerals play important roles in the formation of such microstructures. Analyses of structure-function relationships for matrix proteins in biominerals revealed that almost all matrix proteins have an acidic region for the binding of calcium ion in CaCO3 crystals and interaction domains for other organic molecules. On the other hand, biomineralization of metal nanoparticles by microorganisms were also investigated. Gold nanoparticles and quantum dots containing cadmium were successfully synthesized by bacteria or a fungus. The analyses of components revealed that glycolipids, oligosaccharides, and lactic acids have key roles to synthesize the gold nanoparticle in Lactobacillus casei as reductants and dispersants. These researches about biomineralization will give new insights for material and environmental sciences in the human society.
Assuntos
Exoesqueleto/metabolismo , Biomineralização/fisiologia , Quitina/química , Proteínas da Matriz Extracelular/química , Nanopartículas Metálicas/química , Exoesqueleto/química , Exoesqueleto/ultraestrutura , Animais , Quitina/metabolismo , Quitina/ultraestrutura , Proteínas da Matriz Extracelular/metabolismo , Proteínas da Matriz Extracelular/ultraestrutura , Fusarium/química , Fusarium/fisiologia , Humanos , Lacticaseibacillus casei/química , Lacticaseibacillus casei/fisiologia , Nanopartículas Metálicas/ultraestrutura , Pinctada/anatomia & histologia , Pinctada/fisiologia , Especificidade da EspécieRESUMO
Growth of the cholera bacterium Vibrio cholerae in a biofilm community contributes to both its pathogenicity and survival in aquatic environmental niches. The major components of V. cholerae biofilms include Vibriopolysaccharide (VPS) and the extracellular matrix proteins RbmA, RbmC, and Bap1. To further elucidate the previously observed overlapping roles of Bap1 and RbmC in biofilm architecture and surface attachment, here we investigated the structural and functional properties of Bap1. Soluble expression of Bap1 was possible only after the removal of an internal 57-amino-acid-long hydrophobic insertion sequence. The crystal structure of Bap1 at 1.9 Å resolution revealed a two-domain assembly made up of an eight-bladed ß-propeller interrupted by a ß-prism domain. The structure also revealed metal-binding sites within canonical calcium blade motifs, which appear to have structural rather than functional roles. Contrary to results previously observed with RbmC, the Bap1 ß-prism domain did not exhibit affinity for complex N-glycans, suggesting an altered role of this domain in biofilm-surface adhesion. Native polyacrylamide gel shift analysis did suggest that Bap1 exhibits lectin activity with a preference for anionic or linear polysaccharides. Our results suggest a model for V. cholerae biofilms in which Bap1 and RbmC play dominant but differing adhesive roles in biofilms, allowing bacterial attachment to diverse environmental or host surfaces.
Assuntos
Proteínas de Bactérias/ultraestrutura , Cólera/enzimologia , Proteínas da Matriz Extracelular/ultraestrutura , Conformação Proteica , Vibrio cholerae/enzimologia , Sequência de Aminoácidos/genética , Amiloide/química , Aderência Bacteriana/genética , Proteínas de Bactérias/química , Proteínas de Bactérias/genética , Sítios de Ligação/genética , Biofilmes , Cólera/genética , Cólera/microbiologia , Cristalografia por Raios X , Proteínas da Matriz Extracelular/química , Regulação Bacteriana da Expressão Gênica/genética , Lectinas/química , Metais/química , Polissacarídeos/química , Vibrio cholerae/química , Vibrio cholerae/genética , Vibrio cholerae/patogenicidade , Fatores de Virulência/genéticaRESUMO
Raine syndrome is a rare, autosomal recessive, osteosclerotic bone dysplasia due to pathogenic variants in FAM20C. The clinical phenotype is characterized by generalized osteosclerosis affecting all bones, cerebral calcifications, and craniofacial dysmorphism. Most cases present during the neonatal period with early lethality due to pulmonary hypoplasia and respiratory compromise while only few affected individuals have been reported to survive into adulthood. FAM20C is a ubiquitously expressed protein kinase that contains five functional domains including a catalytic domain, a binding pocket for FAM20A and three distinct N-glycosylation sites. We report a newborn infant with a history of prenatal onset fractures, generalized osteosclerosis, and craniofacial dysmorphism and early lethality. The clinical presentation was highly suggestive of Raine syndrome. A homozygous, novel missense variant in exon 5 of FAM20C (c.1007T>G; p.Met336Arg) was identified by targeted Sanger sequencing. Following in silico analysis and mapping of the variant on a three-dimensional (3D) model of FAM20C it is predicted to be deleterious and to affect N-glycosylation, protein folding, and subsequent secretion of FAM20C. In addition, we reviewed all published FAM20C mutations and observed that most pathogenic variants affect functional regions within the protein establishing evidence for an emerging genotype-phenotype correlation.
Assuntos
Anormalidades Múltiplas/genética , Caseína Quinase I/genética , Fissura Palatina/genética , Anormalidades Craniofaciais/genética , Exoftalmia/genética , Proteínas da Matriz Extracelular/genética , Microcefalia/genética , Osteosclerose/genética , Anormalidades Múltiplas/fisiopatologia , Adulto , Caseína Quinase I/ultraestrutura , Domínio Catalítico/genética , Fissura Palatina/fisiopatologia , Anormalidades Craniofaciais/fisiopatologia , Exoftalmia/fisiopatologia , Proteínas da Matriz Extracelular/ultraestrutura , Regulação da Expressão Gênica no Desenvolvimento/genética , Glicosilação , Homozigoto , Humanos , Lactente , Recém-Nascido , Masculino , Microcefalia/fisiopatologia , Mutação de Sentido Incorreto/genética , Osteosclerose/fisiopatologia , Fenótipo , Ligação Proteica/genética , Dobramento de Proteína , Relação Estrutura-AtividadeRESUMO
The extracellular matrix (ECM) plays a crucial role in embryogenesis, serving both as a substrate to which cells attach and as an active regulator of cell behavior. However, little is known about the spatiotemporal expression patterns and 3D structure of ECM proteins during embryonic development. The lack of suitable methods to visualize the embryonic ECM is largely responsible for this gap, posing a major technical challenge for biologists and tissue engineers. Here, we describe a method of viewing the 3D organization of the ECM using a polyacrylamide-based hydrogel to provide a 3D framework within developing murine embryos. After removal of soluble proteins using sodium dodecyl sulfate, confocal microscopy was used to visualize the 3D distribution of independent ECM networks in multiple developing tissues, including the forelimb, eye, and spinal cord. Comparative analysis of E12.5 and E14.5 autopods revealed proteoglycan-rich fibrils maintain connections between the epidermis and the underlying tendon and cartilage, indicating a role for the ECM during musculoskeletal assembly and demonstrating that our method can be a powerful tool for defining the spatiotemporal distribution of the ECM during embryogenesis.
Assuntos
Desenvolvimento Embrionário , Matriz Extracelular/ultraestrutura , Microscopia Confocal/métodos , Inclusão do Tecido/métodos , Resinas Acrílicas , Animais , Detergentes/farmacologia , Epiderme/ultraestrutura , Proteínas da Matriz Extracelular/efeitos dos fármacos , Proteínas da Matriz Extracelular/ultraestrutura , Corantes Fluorescentes , Membro Anterior/embriologia , Membro Anterior/ultraestrutura , Formaldeído , Hidrogéis , Camundongos , Camundongos Endogâmicos C57BL , Morfogênese , Polímeros , Proteoglicanas/análise , Dodecilsulfato de Sódio/farmacologia , Manejo de Espécimes , Coloração e Rotulagem/métodos , Tendões/embriologia , Tendões/ultraestrutura , Fixação de TecidosRESUMO
Over the last 2 decades, nonlinear imaging methods such as multiharmonic imaging microscopy (MHIM) have become powerful approaches for the label-free visualization of biological structures. Multiharmonic signals are generated when an intense electromagnetic field propagates through a sample that either has a specific molecular orientation or exhibits certain physical properties. It can provide complementary morphological information when integrated with other nonlinear optical imaging techniques such as two-photon excitation (TPE). Here, we present the necessary methodology to implement an integrated approach for multiharmonic and TPE imaging of the mouse aorta using a commercial two-photon microscope. This approach illustrates how to differentiate the microstructure of the mouse aorta that are due to collagen fibrils and elastic laminae under 820 and 1230nm excitation. Our method also demonstrates how to perform multiharmonic generation by reflectance of the forwardly propagating emission signal. The ability to visualize biological samples without additional genetically targeted or chemical stains makes MHIM well suited for studying the morphology of the mouse aorta and has the potential to be applied to other collagen and elastin-rich tissues.
Assuntos
Proteínas da Matriz Extracelular/ultraestrutura , Matriz Extracelular/ultraestrutura , Imagem Molecular/métodos , Imagem Óptica/métodos , Coloração e Rotulagem/métodos , Animais , Matriz Extracelular/química , Proteínas da Matriz Extracelular/química , Camundongos , Imagem Molecular/instrumentação , Imagem Óptica/instrumentação , Coloração e Rotulagem/instrumentaçãoAssuntos
Técnicas de Cultura de Células/métodos , Matriz Extracelular/metabolismo , Coloração e Rotulagem/métodos , Animais , Matriz Extracelular/ultraestrutura , Proteínas da Matriz Extracelular/metabolismo , Proteínas da Matriz Extracelular/ultraestrutura , Imagem Molecular/instrumentação , Imagem Molecular/métodos , Plantas , Fixação de Tecidos/métodosRESUMO
Extracellular matrix microfibrils are critical components of connective tissues with a wide range of mechanical and cellular signalling functions. Collagen VI is a heteromeric network-forming collagen which is expressed in tissues such as skin, lung, blood vessels and articular cartilage where it anchors cells into the matrix allowing for transduction of biochemical and mechanical signals. It is not understood how collagen VI is arranged into microfibrils or how these microfibrils are arranged into tissues. Therefore we have characterised the hierarchical organisation of collagen VI across multiple length scales. The frozen hydrated nanostructure of purified collagen VI microfibrils was reconstructed using cryo-TEM. The bead region has a compact hollow head and flexible tail regions linked by the collagenous interbead region. Serial block face SEM imaging coupled with electron tomography of the pericellular matrix (PCM) of murine articular cartilage revealed that the PCM has a meshwork-like organisation formed from globular densities â¼30nm in diameter. These approaches can characterise structures spanning nanometer to millimeter length scales to define the nanostructure of individual collagen VI microfibrils and the micro-structural organisation of these fibrils within tissues to help in the future design of better mimetics for tissue engineering. STATEMENT OF SIGNIFICANCE: Cartilage is a connective tissue rich in extracellular matrix molecules and is tough and compressive to cushion the bones of joints. However, in adults cartilage is poorly repaired after injury and so this is an important target for tissue engineering. Many connective tissues contain collagen VI, which forms microfibrils and networks but we understand very little about these assemblies or the tissue structures they form. Therefore, we have use complementary imaging techniques to image collagen VI microfibrils from the nano-scale to the micro-scale in order to understand the structure and the assemblies it forms. These findings will help to inform the future design of scaffolds to mimic connective tissues in regenerative medicine applications.
Assuntos
Colágeno Tipo IV/química , Colágeno Tipo IV/ultraestrutura , Microfibrilas/química , Microfibrilas/ultraestrutura , Modelos Químicos , Modelos Moleculares , Simulação por Computador , Proteínas da Matriz Extracelular/química , Proteínas da Matriz Extracelular/ultraestrutura , Conformação ProteicaRESUMO
Lattice corneal dystrophy is associated with painful recurrent corneal erosions and amyloid corneal opacities induced by transforming growth factor ß-induced protein (TGFBIp) that impairs vision. The exact mechanism of amyloid fibril formation in corneal dystrophy is unknown but has been associated with destabilizing mutations in the fourth fasciclin 1 (Fas1-4) domain of TGFBIp. The green tea compound epigallocatechin gallate (EGCG) has been found to inhibit fibril formation of various amyloidogenic proteins in vitro. In this study, we investigated the effect of EGCG as a potential treatment in lattice corneal dystrophy (LCD) using Fas1-4 with the naturally occurring LCD-inducing A546T mutation. A fewfold molar excess of EGCG was found to inhibit fibril formation in vitro by directing Fas1-4 A546T into stable EGCG-bound protein oligomers. Incubation with 2 molar equiv of EGCG led to a 4-fold reduction in the aggregates' membrane disruptive potential, potentially indicative of significantly lower cytotoxicity with regard to corneal erosions. EGCG did not induce oligomer formation by wild-type Fas1-4, indicating that treatment with EGCG would not interfere with the native function of the wild-type protein. Addition of EGCG to 10-day-old fibrils reduced fibril content in a dose-dependent manner. Proteinase K was found to reduce the light scattering of nontreated fibrils by 31% but reduced that of fibrils treated with 8 molar equiv of EGCG by 85%. This suggests that EGCG remodeling of fibril structure can facilitate aggregate removal by endogenous proteases and thus alleviate the protein deposits' light scattering symptoms.
Assuntos
Amiloide/metabolismo , Antioxidantes/farmacologia , Catequina/análogos & derivados , Proteínas da Matriz Extracelular/metabolismo , Fator de Crescimento Transformador beta/metabolismo , Amiloide/química , Catequina/farmacologia , Permeabilidade da Membrana Celular/efeitos dos fármacos , Distrofias Hereditárias da Córnea/tratamento farmacológico , Distrofias Hereditárias da Córnea/metabolismo , Proteínas da Matriz Extracelular/química , Proteínas da Matriz Extracelular/ultraestrutura , Humanos , Lipossomos/metabolismo , Peptídeo Hidrolases/metabolismo , Domínios Proteicos , Multimerização Proteica/efeitos dos fármacos , Proteólise/efeitos dos fármacos , Fator de Crescimento Transformador beta/químicaRESUMO
Enamel is an acellular material formed by the intricate process of amelogenesis. Disruption caused at the initial stages of development, by means of mutations in the ENAM gene encoding the enamelin protein, results in enamel hypoplasia. Little is known about the consequence of ENAM mutation on the enamel structure at a crystallographic level. The aim of this study was to characterize the structure of ENAM-mutated enamel to develop a deeper understanding of the role of enamelin protein during formation with regard to crystal organization. Synchrotron X-ray microdiffraction (SXRD) and scanning electron microscopy (SEM) have been used to measure and correlate enamel crystallography and microstructure in hypoplastic and healthy enamel. Rietveld refinement carried out on 2-dimensional diffraction patterns, collected from the Advanced Photon Source, were used to quantify changes in the preferred orientation (crystallographic texture) within the labial regions of each tooth slice and then correlated with the local microstructure. In general, healthy deciduous incisors displayed a higher degree of crystal organization across the labial surface in comparison with the hypoplastic enamel. ENAM plays the greatest functional role at the enamel-dentine junction (EDJ), as it was the region that exhibited lowest texture relative to unaffected controls. Other areas within the tooth, however, such as the cusp tip, displayed greater organization in line with healthy enamel, suggesting its effects are restricted to the early stages of enamel secretion. Observed clinically, the surface of ENAM-mutated hypoplastic enamel can appear to be normal, yet severe sub-nano and microstructural defects appear beneath the subsurface layer. Quantitative characterization of the crystallographic properties from enamel with known genotype expands the understanding of enamel formation processes and can aid better clinical diagnosis and tailor-made treatment.
Assuntos
Esmalte Dentário/ultraestrutura , Dentina/ultraestrutura , Proteínas da Matriz Extracelular/ultraestrutura , Amelogênese Imperfeita/genética , Amelogênese Imperfeita/patologia , Estudos de Casos e Controles , Cristalografia , Hipoplasia do Esmalte Dentário/patologia , Proteínas da Matriz Extracelular/genética , Humanos , Microscopia Eletrônica de Varredura , Coroa do Dente/ultraestrutura , Dente Decíduo/ultraestrutura , Difração de Raios X/métodosRESUMO
The origin of the age-associated degenerative processes in meniscal tissue is poorly understood and may be related to an imbalance of anabolic and catabolic metabolism. The aim of the current study was to compare medial menisci isolated from juvenile pigs and degenerated medial menisci from adult pigs in terms of gene expression profile and ultrastructure. Medial menisci were isolated from the knee joints of juvenile and adult pigs (n = 8 for each group). Degeneration was determined histologically according to a scoring system. In addition, the gene expression profiles of 14 genes encoding extracellular matrix proteins, catabolic matrix metalloproteinases and mediators of inflammation were analyzed. Changes in the ultrastructure of the collagen network of the meniscal tissue were analyzed by using transmission electron microscopy. The histologic analysis of menisci showed significantly higher grade of degeneration in tissue isolated from adult porcine knee joints compared with menisci isolated from juvenile knee joints. In particular, destruction of the collagen network was greater in adult menisci than in juvenile menisci. Degenerated menisci showed significantly decreased gene expression of COL1A1 and increased expression of MMP2, MMP13, and IL8. The menisci from adult porcine knee joints can serve as a model for meniscal degeneration. Degenerative changes were manifested as differences in histopathology, gene expression and ultrastructure of collagen network.
Assuntos
Regulação da Expressão Gênica , Articulação do Joelho/metabolismo , Articulação do Joelho/ultraestrutura , Meniscos Tibiais/metabolismo , Meniscos Tibiais/ultraestrutura , Sus scrofa , Fatores Etários , Animais , Biópsia , Proteínas da Matriz Extracelular/genética , Proteínas da Matriz Extracelular/metabolismo , Proteínas da Matriz Extracelular/ultraestrutura , Feminino , Perfilação da Expressão Gênica , Mediadores da Inflamação/metabolismo , Masculino , Metaloproteinases da Matriz/genética , Metaloproteinases da Matriz/metabolismo , Microscopia Eletrônica de TransmissãoRESUMO
All inner ear organs possess extracellular matrix appendices over the sensory epithelia that are crucial for their proper function. The tectorial membrane (TM) is a gelatinous acellular membrane located above the hearing sensory epithelium and is composed mostly of type II collagen, and α and ß tectorins. TM molecules self-assemble in the endolymph fluid environment, interacting medially with the spiral limbus and distally with the outer hair cell stereocilia. Here, we used immunogold labeling in freeze-substituted mouse cochleae to assess the fine localization of both tectorins in distinct TM regions. We observed that the TM adheres to the spiral limbus through a dense thin matrix enriched in α- and ß-tectorin, both likely bound to the membranes of interdental cells. Freeze-etching images revealed that type II collagen fibrils were crosslinked by short thin filaments (4±1.5nm, width), resembling another collagen type protein, or chains of globular elements (15±3.2nm, diameter). Gold-particles for both tectorins also localized adjacent to the type II collagen fibrils, suggesting that these globules might be composed essentially of α- and ß-tectorins. Finally, the presence of gold-particles at the TM lower side suggests that the outer hair cell stereocilia membrane has a molecular partner to tectorins, probably stereocilin, allowing the physical connection between the TM and the organ of Corti.
Assuntos
Colágeno Tipo II/metabolismo , Proteínas da Matriz Extracelular/metabolismo , Proteínas de Membrana/metabolismo , Órgão Espiral/metabolismo , Membrana Tectorial/metabolismo , Animais , Colágeno Tipo II/genética , Colágeno Tipo II/ultraestrutura , Matriz Extracelular/metabolismo , Matriz Extracelular/ultraestrutura , Proteínas da Matriz Extracelular/genética , Proteínas da Matriz Extracelular/ultraestrutura , Técnica de Congelamento e Réplica , Proteínas Ligadas por GPI/genética , Proteínas Ligadas por GPI/metabolismo , Proteínas Ligadas por GPI/ultraestrutura , Expressão Gênica , Cobaias , Imuno-Histoquímica , Proteínas de Membrana/genética , Proteínas de Membrana/ultraestrutura , Camundongos , Microscopia Eletrônica de Transmissão , Miosinas/deficiência , Miosinas/genética , Órgão Espiral/ultraestrutura , Ligação Proteica , Ratos , Membrana Tectorial/ultraestruturaRESUMO
Dentin Sialophosphoprotein (DSPP) is the major non-collagenous protein of dentin and plays a significant role in dentin mineralization. Recently, animal models lacking DSPP have been developed and the DSPP KO phenotype has been characterized at the histological level. Little is known, however, about the DSPP KO dentin at nano- and meso-scale. Dentin is a hierarchical material spanning from nano- to macroscale, hence information on the effects of DSPP deficiency at the submicron scale is essential for understanding of its role in dentin biomineralization. To bridge this gap, we have conducted ultrastructural studies of dentin from DSPP KO animals. Transmission electron microscopy (TEM) studies of DSPP KO dentin revealed that although the overall ultrastructural organization was similar to the WT, the mineral particles were less organized. Scanning electron microscopy in the back-scattered mode (BS-SEM) of the DSPP KO dentin revealed that circumpulpal dentin comprises large areas of non-mineralized matrix, with numerous spherulitic mineralized inclusions, while the mantle dentin appeared largely unaffected. Analysis of the mineral distribution in the circumpulpal dentin of the DSPP KO mice suggests a reduction in the number of mineral nucleation sites and an increase in the nucleation barrier in DSPP KO dentin. These preliminary results indicate that in addition to the reduction of mineralized and total dentin volume in DSPP KO animals significant changes in the ultrastructural organization exist. These changes are likely related to the role of DSPP in the regulation of mineral formation and organization in dentin.
Assuntos
Dentina/ultraestrutura , Dentinogênese/fisiologia , Proteínas da Matriz Extracelular/deficiência , Proteínas da Matriz Extracelular/ultraestrutura , Fosfoproteínas/deficiência , Fosfoproteínas/ultraestrutura , Sialoglicoproteínas/deficiência , Sialoglicoproteínas/ultraestrutura , Calcificação de Dente/fisiologia , Animais , Camundongos , Camundongos Knockout , Microscopia Eletrônica de Varredura , Microscopia Eletrônica de Transmissão , FenótipoRESUMO
Differentiation of enteric neural stem cells into several appropriate neural phenotypes is crucial while considering transplantation as a cellular therapy to treat enteric neuropathies. We describe the formation of tissue engineered innervated sheets, where intestinal smooth muscle and enteric neuronal progenitor cells are brought into close association in extracellular matrix (ECM) based microenvironments. Uniaxial alignment of constituent smooth muscle cells was achieved by substrate microtopography. The smooth muscle component of the tissue engineered sheets maintained a contractile phenotype irrespective of the ECM composition, and generated equivalent contractions in response to potassium chloride stimulation, similar to native intestinal tissue. We provided enteric neuronal progenitor cells with permissive ECM-based compositional and viscoelastic cues to generate excitatory and inhibitory neuronal subtypes. In the presence of the smooth muscle cells, the enteric neuronal progenitor cells differentiated to functionally innervate the smooth muscle. The differentiation of specific neuronal subtypes was influenced by the ECM microenvironment, namely combinations of collagen I, collagen IV, laminin and/or heparan sulfate. The physiology of differentiated neurons within tissue engineered sheets was evaluated. Sheets with composite collagen and laminin had the most similar patterns of Acetylcholine-induced contraction to native intestinal tissue, corresponding to an increased protein expression of choline acetyltransferase. An enriched nitrergic neuronal population, evidenced by an increased expression of neuronal nitric oxide synthase, was obtained in tissue engineered sheets that included collagen IV. These sheets had a significantly increased magnitude of electrical field stimulated relaxation, sensitive maximally to nitric oxide synthase inhibition. Tissue engineered sheets containing laminin and/or heparan sulfate had a balanced expression of contractile and relaxant motor neurons. Our studies demonstrated that neuronal subtype was modulated by varying ECM composition. This observation could be utilized to derive enriched populations of specific enteric neurons in vitro prior to transplantation.
Assuntos
Proteínas da Matriz Extracelular/metabolismo , Músculo Liso/inervação , Músculo Liso/fisiologia , Células-Tronco Neurais/citologia , Neurogênese , Engenharia Tecidual/métodos , Animais , Células Cultivadas , Colágeno/metabolismo , Colágeno/ultraestrutura , Proteínas da Matriz Extracelular/ultraestrutura , Heparitina Sulfato/metabolismo , Laminina/metabolismo , Laminina/ultraestrutura , Contração Muscular , Músculo Liso/citologia , Células-Tronco Neurais/metabolismo , Neurônios/citologia , Neurônios/metabolismo , CoelhosRESUMO
Cell-extracellular matrix interaction plays a major role in maintaining the structural integrity of connective tissues and sensing changes in the biomechanical environment of cells. Collagen VI is a widely expressed non-fibrillar collagen, which regulates tissues homeostasis. The objective of the present investigation was to extend our understanding of the role of collagen VI in human ACL. This study shows that collagen VI is associated both in vivo and in vitro to the cell membrane of knee ACL fibroblasts, contributing to the constitution of a microfibrillar pericellular matrix. In cultured cells the localization of collagen VI at the cell surface correlated with the expression of NG2 proteoglycan, a major collagen VI receptor. The treatment of ACL fibroblasts with anti-NG2 antibody abolished the localization of collagen VI indicating that collagen VI pericellular matrix organization in ACL fibroblasts is mainly mediated by NG2 proteoglycan. In vitro mechanical strain injury dramatically reduced the NG2 proteoglycan protein level, impaired the association of collagen VI to the cell surface, and promoted cell cycle withdrawal. Our data suggest that the injury-induced alteration of specific cell-ECM interactions may lead to a defective fibroblast self-renewal and contribute to the poor regenerative ability of ACL fibroblasts.
Assuntos
Ligamento Cruzado Anterior/metabolismo , Membrana Celular/metabolismo , Colágeno Tipo VI/metabolismo , Proteínas da Matriz Extracelular/metabolismo , Ligamento Cruzado Anterior/ultraestrutura , Comunicação Celular , Membrana Celular/ultraestrutura , Colágeno Tipo VI/ultraestrutura , Tecido Conjuntivo/metabolismo , Tecido Conjuntivo/ultraestrutura , Matriz Extracelular/metabolismo , Matriz Extracelular/ultraestrutura , Proteínas da Matriz Extracelular/ultraestrutura , Fibroblastos/citologia , Fibroblastos/metabolismo , Humanos , Estresse MecânicoRESUMO
Biofilms are surface-associated groups of microbial cells that are embedded in an extracellular matrix (ECM). The ECM is a network of biopolymers, mainly polysaccharides, proteins, and nucleic acids. ECM proteins serve a variety of structural roles and often form amyloid-like fibers. Despite the extensive study of the formation of amyloid fibers from their constituent subunits in humans, much less is known about the assembly of bacterial functional amyloid-like precursors into fibers. Using dynamic light scattering, atomic force microscopy, circular dichroism, and infrared spectroscopy, we show that our unique purification method of a Bacillus subtilis major matrix protein component results in stable oligomers that retain their native α-helical structure. The stability of these oligomers enabled us to control the external conditions that triggered their aggregation. In particular, we show that stretched fibers are formed on a hydrophobic surface, whereas plaque-like aggregates are formed in solution under acidic pH conditions. TasA is also shown to change conformation upon aggregation and gain some ß-sheet structure. Our studies of the aggregation of a bacterial matrix protein from its subunits shed new light on assembly processes of the ECM within bacterial biofilms.
Assuntos
Bacillus subtilis/fisiologia , Proteínas de Bactérias/química , Biofilmes , Proteínas da Matriz Extracelular/química , Adsorção , Silicatos de Alumínio/química , Amiloide/química , Amiloide/isolamento & purificação , Amiloide/ultraestrutura , Proteínas de Bactérias/isolamento & purificação , Proteínas de Bactérias/ultraestrutura , Proteínas da Matriz Extracelular/isolamento & purificação , Proteínas da Matriz Extracelular/ultraestrutura , Concentração de Íons de Hidrogênio , Interações Hidrofóbicas e Hidrofílicas , Luz , Microscopia de Força Atômica , Tamanho da Partícula , Multimerização Proteica , Espalhamento de Radiação , Espectroscopia de Infravermelho com Transformada de Fourier , Propriedades de SuperfícieRESUMO
BACKGROUND: Proteases expressed in atherosclerotic plaque lesions generate collagen fragments, release glycosaminoglycans (chondroitin sulfate [CS] and dermatan sulfate [DS]) and expose extracellular matrix (ECM) proteins (e.g. decorin) at sites of fibrin formation. OBJECTIVE: Here we address the effect of these vessel wall components on the lysis of fibrin by the tissue plasminogen activator (tPA)/plasminogen system and on the mechanical stability of clots. METHODS AND RESULTS: MMP-8-digested collagen fragments, isolated CS, DS, glycosylated decorin and its core protein were used to prepare mixed matrices with fibrin (additives present at a 50-fold lower mass concentration than fibrinogen). Scanning electron microscopy (SEM) showed that the presence of ECM components resulted in a coarse fibrin structure, most pronounced for glycosylated decorin causing an increase in the median fiber diameter from 85 to 187 nm. Rheological measurements indicated that these structural alterations were coupled to decreased shear resistance (1.8-fold lower shear stress needed for gel/fluid transition of the clots containing glycosylated decorin) and rigidity (reduction of the storage modulus from 54.3 to 33.2 Pa). The lytic susceptibility of the modified fibrin structures was increased. The time to 50% lysis by plasmin was reduced approximately 2-fold for all investigated ECM components (apart from the core protein of decorin which produced a moderate reduction of the lysis time by 25%), whereas fibrin-dependent plasminogen activation by tPA was inhibited by up to 30%. CONCLUSION: ECM components compromise the chemical and mechanical stability of fibrin as a result of changes in its ultrastructure.