Potential Antidiabetic, Antioxidative and Antiproliferative Properties of Functional Wheat Flour Muffins Enriched with White Clover Flowers (Trifolium repens L.).

The aim of the study was to evaluate the functional properties of muffins fortified with white clover flowers (Trifolium repens L.), which were added to the dough in the following amounts: (i) 0% (control); (ii) 2.5%; (iii) 5.5%; (iv) 7.5%; and (v) 10%. The organoleptic properties were assessed by a panel of consumers. Additionally, the following parameters were also tested: basic chemical composition, total polyphenols, the antioxidant activity together with antiproliferative effects on the A375 melanoma cell line, starch nutritional fractions and the in vitro glycemic index. As a result, replacing wheat flour with white clover flour significantly affected the color, aroma and taste of the muffins. The content of proteins, fats, total ash, dietary fiber, resistant starch (RS), slowly digestible starch (SDS),total polyphenols and antioxidant activity increased statistically significantly with the elevated amount of white clover flour added to the dough. At the same time, the content of free glucose (FG), rapidly available glucose (RAG) and rapidly digestible starch (RDS), the value of the in vitro glycemic index and the viability of melanoma cancer cells decreased significantly. The muffins enriched with white clover flowers might constitute an interesting proposition and extension of the existing assortment of confectionery products.

Young Shoots of Red Beet and the Root at Full Maturity Inhibit Proliferation and Induce Apoptosis in Breast Cancer Cell Lines.

Modern medicine is struggling with the problem of fully effective treatment of neoplastic diseases despite deploying innovative chemotherapeutic agents. Therefore, undertaking cancer-prevention measures, such as proper eating habits, should be strongly recommended. The present research aimed to compare the effects of juice from young shoots of beetroot compared to juice from root at full maturity on human breast cancer and normal cells. The juice from young shoots, both in the native and digested form, was most often a significantly stronger inhibitor of the proliferation of both analyzed breast cancer cell lines (MCF-7 and MDA-MB-231), compared to the native and digested juice from red beetroot. Regardless of juice type, a significantly greater reduction was most often shown in the proliferation of estrogen-dependent cells (MCF-7 line) than of estrogen-independent cells (MDA-MB-231 line). All analyzed types of beetroot juice and, in particular, the ones from young shoots and the root subjected to digestion and absorption, exerted an antiproliferative and apoptotic effect (pinpointing the internal apoptosis pathway) on the cells of both cancer lines studied. There is a need to continue the research to comprehensively investigate the factors responsible for both these effects.

Antioxidative Properties and Acrylamide Content of Functional Wheat-Flour Cookies Enriched with Wild-Grown Fruits.

This study aimed to evaluate the impact of widely grown fruits (wild roses, elderberries, sea buckthorns, rowans, chokeberries, and hawthorns) as a functional ingredient in wheat-flour cookie formulation on antioxidative properties with a simultaneous reduction of the carcinogen-like compound acrylamide. The organoleptic features of the cookies were assessed by a panel of consumers. The following parameters were measured: chemical composition, total polyphenols, polyphenolic profile, antioxidant activity, and acrylamide content. The overall ratings of the tested cookies with the addition of chokeberries, hawthorns, sea buckthorns, and elderberries were more than satisfactory, while wild rose and rowan cookies were the most widely accepted and best rated by the panelists. The antioxidant activity of the tested cookies was 1.1−15.22 µmol trolox·g−1 dm and 2.46−26.12 µmol Fe (II)·g−1 dm as measured by the ABTS and FRAP methods, respectively. All the fruit-enriched cookies had significantly higher antioxidative properties (p < 0.05) in comparison to the control cookies, but among the fruit-enriched cookies, there were differences in the quality and quantity of particular polyphenols. The acrylamide content was significantly decreased by 59% (hawthorn), 71% (rowan), 87% (wild rose), 89% (sea buckthorn), 91% (elderberry), and 94% (chokeberry) compared with the control cookies (p < 0.05). Cookies enriched with wild-grown fruits could constitute a promising novel snack food.

PPAR Receptors Expressed from Vectors Containing CMV Promoter Can Enhance Self-Transcription in the Presence of Fatty Acids from CLA-Enriched Egg Yolks-A Novel Method for Studies of PPAR Ligands.

PPAR receptors are ligand-dependent transcription factors activated in response to various small lipophilic ligands controlling the expression of different genes involved in cellular differentiation, development, metabolism, and tumorigenesis. Unexpectedly, our previous studies have shown that single plasmid-based expression of PPARs under the control of CMV promoter/enhancer was significantly elevated in the presence of PPAR agonists. Here we show that the PPAR reporters controlled by the CMV promoter/enhancer, that was shown to contain three internal non-canonical PPRE elements, can be used as a fast screening system for more effective PPAR ligands. This model allowed us to confirm our previous results indicating that fatty acids of CLA-enriched egg yolks (EFA-CLAs) are efficient PPAR ligands that can specifically upregulate the expression of PPARα and PPARγ leading to downregulation of MCF-7 cancer cell proliferation. We also show that synthetic cis9,trans11CLA is more effective in transactivation of PPARγ, while trans10,cis12CLA of PPARα receptor indicating the selectivity of the CLA isomers. This report presents a novel, fast, and reliable strategy for simple testing of PPAR ligands using PPAR expressing plasmids containing the CMV promoter/enhancer that can trigger the positive feedback loop of PPAR self-transcription in the presence of PPAR ligands.

Fructan-Enriched Diet Increases Bone Quality in Female Growing Rats at Calcium Deficiency.

This study was designed to determine the effect of feeding female rats with low-calcium diet containing one of three fructan sources (Jerusalem artichoke, yacon, Beneo Orafti Synergy1), on selected bone parameters. Growing Wistar rats were fed modified AIN-93 G diet enriched in fructan sources (8%), added alone or as a strawberry sorbet ingredient. Two of eight groups were a validation model, where the positive control group was fed with recommended calcium dose in the diet (RCD), and negative one - with low calcium diet (LCD). After 12 weeks, femoral Ca content, bone densitometry, architecture and hardness were examined. The positive effects on femoral Ca content and cortical thickness, area and content in distal part of bone was observed after feeding animals diet enriched in Jerusalem artichoke sorbet. Beneficial action on other bone tomographic parameters (particularly trabecular volumetric bone mineral density) in this part of femur were associated mainly with the consumption of the diet with sorbet containing yacon. Our results showed an important role of diet containing frozen strawberry desserts enriched in fructan sources in the maintenance of healthy bones of growing organism. It may suggest possible synergisms between fructans and bioactive substances of strawberry.

Intake of Vitamin C, ß-Carotene, and Polyphenolic Compounds by Children and Adolescents from Orphanages.

OBJECTIVE: The objective of this study was analytical assessment of the concentration of selected antioxidants as well as total antioxidant capacity of daily diets of children and adolescents from orphanages in Krakow (Poland). METHODS: Daily diets of children and adolescents were collected in 4 seasons of the year from 7 orphanages located in Krakow. The concentrations of vitamin C, ß-carotene, polyphenolic compounds, and antioxidant capacity were measured. RESULTS: The lowest content of vitamin C in diets of children and adolescents was measured in diets collected in autumn. The estimated average requirement (EAR) was in the range 49.0%-76.4%. In winter the content of this compound was significantly higher in daily diets (113.9%-192.0% coverage of EAR) compared to the level of this nutrient in daily diets from other seasons. The content of ß-carotene, lower than 50% of EAR value, calculated as the vitamin A equivalent, has been demonstrated in 4 orphanages in summer and in one in spring and autumn. The highest mean content of this antioxidant in daily diets was found in winter, when ß-carotene intake met recommendations in the range 131%-368%. The mean concentration of polyphenolic compounds per year was in the range 301.5-537.5 mg/person/day. It has been observed that diets during the winter and autumn generally provide higher amounts of polyphenols than in other seasons. The highest antioxidant activity was measured in diets collected during the winter season. CONCLUSIONS: The results indicate that the diets of children and adolescents should contain more fresh vegetables and fruits, which are good source of natural antioxidants.

The effects of peeling and cooking on the mineral content and antioxidant properties in carrots enriched with potassium iodate and/or selenite (Se(IV)) and selenite (Se(VI)).

Research covered six variants: control, unfertilized carrots and carrots fertilized with: KIO3, Na2SeO4, Na2SeO3, KIO3 and simultaneously with Na2SeO4, and fertilized with KIO3 and simultaneously Na2SeO3. Carrots enriched with iodate or selenite, or both iodate and selenite, were characterized by higher amount of these minerals. Changes to the content of micro- and macroelements, during the cooking time of the carrots, both in peeled and unpeeled carrots, did not head in the same direction (increase, decrease and no change). However, cooking an unpeeled carrot generally resulted in the increased content of polyphenol and carotenoids. On the other hand, cooking peeled carrots led to a decrease in the content of polyphenol and a general lack of change in carotenoid content in relation to the unpeeled cooked carrot. During cooking, the antioxidant activity of the carrot being assessed changed together with the direction of changes in polyphenol content but not in line with the direction of changes in carotenoids.

Assessment of nutritional habits and preferences of children and adolescents brought up in Krakow's orphanages.

BACKGROUND: Proper nutrition can not only guarantee harmonious child's development, but can also protect against later development of diet-related diseases such as type-2 diabetes, obesity or cardiovascular diseases. Many diseases as well as disorders of intellectual development of a young people may result from the mistakes made in a period of an intense growth and maturing of a child. OBJECTIVE: The aim of this study was to assess nutritional habits and preferences in subjects brought up in orphanages in terms of subjective evaluation of eating patterns, frequency of the meal consumption, and a concern about healthy life style. MATERIAL AND METHODS: This study has been conducted to evaluate nutritional habits and preferences of 181 children aged 9-20, from orphanages in Krakow. Study was performed in 2007-2008 in 5 orphanages, which were under the control of Social Welfare Centre. Evaluation of nutritional habits and preferences was performed on the basis of an anonymous 8-question questionnaire adjusted to the respondent's age, which referred, among other, to questions about subjective assessment of eating patterns, frequency of meal consumption and the concern about healthy lifestyle patterns. RESULTS: About 75% of the examined population responded affirmatively to a question about subjective assessment of proper eating patterns; 80% female and 88% male respondents declared a regular consumption of meals. Daily diets of children in orphanages consisted usually from 5 or 4 meals (respectively 38 and 33% of affirmative responses); although, 5 meals were most often eaten by the youngest children. Furthermore, it has been revealed that milk and dairy products were consumed, on average, by 66% of respondents. On average, 76% of respondents reported everyday consumption of the lean meat, while 72% ate fish at least once per week. CONCLUSIONS: Results obtained in this study, identify the improper nutritional habits and indicate the necessity of intervention, consisting on training courses and workshops for children, adolescents and their teachers.

Assessment of the frequency of snack and beverages consumption and stimulants intake in children grown up in orphanages in Krakow.

BACKGROUND: Childhood is a particular period of life, when nutritional habits are emerging, so much attention should be paid to proper dietary habits, which become a nutritional pattern copied in the future. OBJECTIVE: The aim of this study was to evaluate selected dietary habits and preferences in a group of children living in Krakow orphanages (supervising by the Social Welfare Centre in Krakow), by assessing the frequency of snacks and beverages consumptions as well as an intake of beverages with caffeine, alcohol and smoking cigarettes. MATERIAL AND METHODS: Studies were performed in the years 2007-2008 in five orphanages located in Krakow. 181 children, 9 to 20 yrs of age, participated in this study. Assessment of dietary habits and preferences was performed based on anonymous questionnaire which included questions about snacking frequency, favorite and most frequently drank beverages without or with caffeine, alcohol and cigarettes smoking. RESULTS: The analysis of nutritional habits showed that the most popular high-calorie snacks were eaten by the youngest children. Children and adolescents asked for the most commonly consumed beverages indicated on fizzy drinks and fruits juice. Among the youngest children (9-12 years old), 5% reported drinking alcohol, 10% of boys smoked cigarettes, 10% of girls and 21% of boys drank coffee. Among the oldest respondents, about 35% declared drinking coffee, 39% girls and 65% boys declared smoking cigarettes, whereas drinking alcohol reported 22 and 38% subjects, respectively. CONCLUSIONS: Results obtained in this study, indicate the necessity of intervention, consisting on running training courses and workshops of the assessed population, i.e. children, adolescents and instructing their educators.

Anthropometric assessment of the nutritional status of children and adolescents residing in selected Polish orphanages based on their energy intake and physical activity level.

BACKGROUND: Actions to the prevention of overweight and obesity should be first addressed to the youngest population and their parents, guardians as well as teachers. The major objectives of prevention and treatment of overweight and obesity programme should be focused on modification of nutritional habits and promotion of physical activity. OBJECTIVES: The aim of this study was to evaluate the nutritional status, intake of energy and macronutrients as well as the physical activity of students from orphanages in Krakow. MATERIAL AND METHODS: Study was performed in 5 orphanages located in Krakow (Poland), which were under control of Social Welfare Centre in Krakow. The study involved 153 students, 67 girls and 86 boys, aged from 7 to 20 years. Nutritional status was assessed by anthropometric measurements. The protein and total fat content in diets was measured by chemical analyses and carbohydrates were calculated by difference. Physical activity level of children and adolescents was assessed by questionnaire. RESULTS: Over 80% of boys and about 90% of girls had a normal body mass. Students have spent their free time on additional physical activity from 1h 34 min/day to 5 h 12 min/day. They also have spent their free time on sedentary activities on average 4 h/day. Daily diets of students did not met recommendations for energy, carbohydrates and fats. Intake of protein was too high and exceeded the estimated average requirement even over three times. CONCLUSIONS: Despite the insufficient intake of fat and carbohydrates, students generally showed a proper BMI value. This suggests that excess intake of protein was used for maturation process and was additional source of energy. Reported additional physical activity was satisfactory.

Chemical analysis of minerals content in daily diets of children and adolescents grown up in Krakow orphanages.

BACKGROUND: Numerous studies attest to the role that inappropriate food product groups in the human diet, including children and adolescents diet, leading to abnormal intakes of many important nutrients, such as minerals. OBJECTIVES: These studies were undertaken to assess the coverage of the recommended values for chosen minerals by 205 children and adolescents between the ages of 4 and 20 years, grown up in Krakow orphanages. MATERIALS AND METHODS: Sodium, magnesium, zinc, iron, copper, calcium and potassium concentrations in 112 diets of children and adolescents from 7 selected orphanages in Krakow in every season of the year were performed using AAS method. Results obtained were compared to recommended standards in Poland. RESULTS: Intakes of calcium and potassium were usually too low whilst adequate levels of dietary magnesium and calcium were seen only for the youngest children. Recommended dietary levels of zinc were met in all cases and mostly also for iron and copper. Sodium intakes were however excessive. CONCLUSIONS: The study demonstrates that regular a monitoring and adjusting of the diet is necessary for the assessed children and adolescents in order to correct dietary abnormalities.

Relationship between the intake of energy and basic nutrients and the BMI values in group of children aged 10-12.

BACKGROUND: According to the WHO data in January 2007, 14 million children were overweight in the countries of the European Union. The problem of childhood obesity should be considered as one of the biggest challenges facing twenty- first century nutrition and pediatrics. OBJECTIVE: The aim of this study was to show the correlation between energy, basic nutrients intake and BMI of school children aged 10-12 years, using Pearson's correlation coefficient. MATERIALS AND METHODS: 169 pupils (69 from Krakow and 100 from Skawina), aged 10-12, participated in these studies. The studies were approved by the Board of Education, school principals and the parents. On the basis of measurements of weight and height, body mass index (BMI) was calculated. 24 h dietary recalls were collected in the spring and autumn of 2006, for 4 selected days per week. RESULTS: Intake of energy, total carbohydrate and fat did not met requirements, but intake of protein was too high. Proper weight, based on BMI values in the population aged 10-12, were found in 74.9% of boys and 81.2% of girls. Deficiencies in body weight occurred in 4.2% and 3.4%, overweight in 15.6% and 10.0% while obesity in 5.3% and 5.4% of respondents. CONCLUSIONS: Inverse correlation was found between the implementation of standards for energy and BMI values of children. It was observed positive correlation between the consumption of total and animal protein and BMI value. KEY WORDS: children, underweight, obesity, BMI, nutrients, nutrition, Pearson's correlation coefficient.

What Determinants Are Related to Milk and Dairy Product Consumption Frequency among Children Aged 10-12 Years in Poland? Nationwide Cross-Sectional Study.

Due to their high nutritional value, milk and dairy products should be a permanent element of a properly balanced diet for children and adolescents. The study aimed to identify (i) the frequency of milk and dairy product consumption by children aged 10-12 years in the opinion of children and their parents and (ii) the determinants related to the consumption of these products (including lifestyle, nutrition knowledge, and the nutritional status of children's as well as parents' nutrition knowledge). A cross-sectional study was conducted with 12,643 primary school students aged 10-12 and 7363 parents. Dietary data were collected using the Food Frequency Consumption and Nutritional Knowledge Questionnaire (SF-FFQ4PolishChildren® and KomPAN®). Anthropometric measurements were taken and body mass index (BMI) and waist/height ratios (WHtR) were calculated. A logistic regression model was used to assess the likelihood of the frequent consumption of dairy products in the opinion of both the children and their parents, and the quality of the obtained models was assessed using model fit statistics and the Hosmer and Lemeshow test. The frequency of consuming milk and milk products (every day and more) was low in the opinion of children aged 10-12 years (29.6%). According to their parents, the frequency of the consumption of dairy products was slightly higher (44.8%). Six factors associated with the frequency of milk and dairy product consumption were identified independently. These included schoolchildren and their parents' nutrition knowledge, physical activity, sleep, gender, and place of residence. More of these products were consumed by children with greater nutrition knowledge-both their own and their parents'-higher physical activity, longer sleep duration, males, and those living in the city. Family eating habits, particularly eating meals together, also played an important role in the consumption of milk and dairy products. According to the parents, the children in older grades were less likely to consume dairy products more frequently. The obtained results suggest an insufficient consumption of milk and dairy products. The identification of modifiable factors, such as nutrition knowledge, physical activity, sleep duration, and eating meals with the family, suggests the need to improve the existing strategies, including activities encouraging nutritional education for both schoolchildren and their parents.

Assessment of polyphenols, beta-carotene, and vitamin C intake with daily diets by primary school children.

The aim of this study was to analytically evaluate the daily intake of total polyphenols, ß-carotene, and vitamin C by 10- to 12-year-old children. Twenty four-hour dietary recalls were collected in spring and autumn 2006 from students in Krakow and Skawina (Malopolska region, Poland). The content of vitamin C, polyphenols, and ß-carotene was determined in reconstructed daily diets. Statistical analysis did not show any significant differences between the mean intake of measured compounds according to school location, gender, or season. Diets of children contained adequate amounts of polyphenols in relation to the recommended values. Intake of ß -carotene usually exceeded the recommended retinol equivalent; in contrast, vitamin C did not meet requirements. Increased consumption of fruits and vegetables rich in vitamin C is recommended.

Curly Kale (Brassica oleracea var. Sabellica L.) Biofortified with 5,7-Diiodo-8-quinolinol: The Influence of Heat Treatment on Iodine Level, Macronutrient Composition and Antioxidant Content.

Many disorders are a result of an inadequate supply of macronutrients and micronutrients in the diet. One such element is iodine. This study used curly kale (Brassica oleracea var. Sabellica L.) biofortified with the 5,7-diiodo-8-quinolinol iodine compound. The effect of the heat treatment on the chemical composition of the curly kale was studied. In addition, iodine bioavailability was evaluated in in vivo studies. Our investigation showed that iodine loss depends on the type of heat treatment as well as on the variety of kale. Curly kale biofortified with iodoquinoline had significantly higher iodine levels after thermal processing (steaming, blanching, boiling) than the vegetable biofortified with KIO3. Generally, steaming was the best thermal processing method, as it contributed to the lowest iodine loss in curly kale. The red variety of kale, 'Redbor F1', showed a better iodine stability during the heat treatment than the green variety, 'Oldenbor F1'. The thermal treatment also significantly affected the dry matter content and the basic chemical composition of the tested varieties of the 5,7-diI-8-Q biofortified kale. The steaming process caused a significant increase in total carbohydrates, fiber, protein and crude fat content ('Oldenbor F1', 'Redbor F1'), and antioxidant activity ('Oldenbor F1'). On the other hand, boiling caused a significant decrease, while steaming caused a significant increase, in protein and dry matter content ('Oldenbor F1', 'Redbor F1'). The blanching process caused the smallest significant decrease in ash compared to the other thermal processes used ('Oldenbor F1'). A feeding experiment using Wistar rats showed that iodine from the 5,7-diI-8-Q biofortified kale has a higher bioavailability than that from the AIN-93G diet. A number of promising results have been obtained, which could form the basis for further research.

The beneficial effects of Brassica vegetables on human health.

The products of plant origin are a rich source of biologically active substances, both nutritive and referred as anti-nutritive. A large group of these compounds are substances with antioxidant activity that fights against free radicals. In the family of Brassicaceae vegetables, Brassica, is the largest and most widely consumed a group of plants in Europe and all over the world. They are characterized by different levels of nutrients. However because of their large and frequent consumption, they may become a significant source of nutrients and bioactive compounds in the daily diet. The beneficial effects of Brassica vegetables on human health have been somewhat linked to phytochemicals. They prevent oxidative stress, induce detoxification enzymes, stimulate immune system, decrease the risk of cancers, inhibit malignant transformation and carcinogenic mutations, as well as, reduce proliferation of cancer cells. Brassica vegetables contain a lot of valuable metabolites, which are effective in chemoprevention of cancer, what has been already documented by numerous studies. Due to the presence of vitamins C and E, carotenoids and antioxidant enzymes such as catalase, superoxide dismutase (SOD) and peroxidase, these vegetables are considerable source ofantioxidants, and due to the presence of polyphenols and the sulfur-organic compounds exert also antimutagenic action. Moreover, these vegetables are also rich in glucosinolates, which are unstable compounds and undergo degradation into biologically active indoles and isothiocyanates under the influence of enzyme presented in plant tissues- myrosynase. These substances through the induction of enzymatic systems I and II phase of xenobiotics metabolism may affect the elimination or neutralization of carcinogenic and mutagenic factors, and consequently inhibit DNA methylation and cancer development. Despite many healthy benefits upon eating of cruciferous vegetables, it has been also seen a negative impact of their certain ingredients on the human body.

[Frequency and preferences of consumption of high-fat products by students of catering school in Kraków]. / Czestotliwosc i preferencje spozycia produktów wysokotluszczowych przez uczniów zespolu szkól gastronomicznych w krakowie.

BACKGROUND: Dyslipidemia is one of the most important risk factors for atherosclerotic disease and may lead to coronary heart disease, obesity, type II diabetes and certain cancers. The choice of food and meals by adults is a large part determined by the dietary habits and knowledge acquired in earlier periods of life. OBJECTIVE: The aim of this study was to evaluate frequency of consumption of food products containing fats among students of the Catering School in Kraków. MATERIAL AND METHODS: The study was conducted with the participation of 140 students divided into two subgroups, depending on gender and age, in the autumn and winter season. During the studies a food frequency questionnaire containing question about frequency intake of selected groups of food products containing fats was used. This questionnaire was prepared at the Department of Human Nutrition Agricultural University of Kraków. RESULTS: A significant (P <0.05) effect of gender on preferences for the choice of milk, eating greasy food and the type of fat used for frying foods was found. Girls more often chose milk with reduced fat content compared to boys. It was observed that the fatty parts of food more often were eliminated by girls than boys. Girls more often than boys chose to fry foods oil or margarine, while boys more often used lard and bacon. Frequency of consumption offish or fish products, milk consumption preferences and eating fatty foods depended significantly (P <0.05) oon age. Older students less likely to eat the fish or fish products more often left fatty cuts compared to the younger students. Fatty milk was likely consumed by younger students, while fat free milk by older youth. CONCLUSIONS: It was found that the students of the Catering School, despite the acquired knowledge of nutrition, make many mistakes.

[Assessment of intake of minerals with daily diets by children aged 10-12 years from Malopolska region]. / Ocena pokrycia zapotrzebowania na skladniki minerlane przez dzieci w wieku 10-12 lat z regionu Malopolski.

BACKGROUND: Adolescence is a time of rapid growth and development of the organism. During intense growth and maturation changes taking place in the body, it cost higher demand for energy and nutrients including minerals. OBJECTIVE: The aim of this study was to assess the intake of sodium, potassium, calcium, phosphorus, magnesium, iron, zinc, copper and manganese with daily diets by children aged 10-12 years. MATERIALS AND METHODS: Assessment of intake of mineral compounds from daily diets was conduced with using 24 hours recall. Data were collected in spring and autumn of 2006 in randomly selected primary schools in Krakow and Skawina. Average intake of minerals (Na, K, Ca, P, Mg, Fe, Zn, Cu, Mn) with daily diets by respondents and the meeting of requirements was estimated with using the computer softwere Diet 2. RESULTS: In almost all cases intake of minerals with daily diets did not meet requirements. Daily dies of children did not met requirements for major minerals i.e. calcium and magnese, and for trace minerals copper, zinc, iron. Insufficient intake of calcium, while fully covering the demand for phosphorus, resulted in improper ratio these minerals in the diet (0.62 at the recommended, of 1). Intake of manganese and potassium fully covered demand, while the sodium and salt were taken in excess. CONCLUSIONS: To low intake of minerals compounds by children may increase the risk of development of diet non-communicable diseases.

Comparison of calcium and magnesium contents in cruciferous vegetables grown in areas around steelworks, on organic farms, and those available in retail.

The aim of the present study was to compare calcium and magnesium contents in cruciferous vegetables grown under diversified ecological conditions for three consecutive years, independently of the climatic and agrotechnical conditions. The metal contents were determined using validated Atomic Absorption Spectrometry with atomization in the flame (FAAS method; spectrometer: AA240FS Varian). The dry mass of various vegetable species cultivated on organic farms contained, in most cases, significantly higher or similar calcium and magnesium amounts in comparison with those from farms located in the closest vicinity of steelworks and those purchased at local markets. Cruciferous vegetables from the two latter sources showed comparable contents of the essential minerals under study.

Comparison of lead and cadmium contents in cruciferous vegetables grown under diversified ecological conditions: Cracow region of Poland.

The aim of the present study was to compare lead and cadmium contents in cruciferous vegetables grown under diversified ecological conditions for three consecutive years, independently of the climatic and agrotechnical conditions. The research was conducted in the Cracow region of Poland and tests vegetables near the Steelworks, from ecological farms, and from local markets. The heavy metal contents were determined using the validated Atomic Absorption Spectrometry method, including electrothermal atomization, with an ET-AAS graphite cuvette (Varian AA240Z, made by Varian). Cruciferous vegetables cultivated in the areas surrounding the steelworks were characterized by alarmingly high lead content versus ecological and commercially available vegetables, while the contents of this metal in vegetables from the two latter locations did not differ. It cannot be definitively stated that the origin of vegetables influenced their cadmium content.