Recovery of microelements from municipal sewage sludge by reed canary grass and giant miscanthus.

The aim of the study was to evaluate the content, uptake, and utilization of Al, Mn, Fe, Co, and Mo from municipal sewage sludge by the reed canary grass (RCG) and the giant miscanthus (MG). The impact of sewage sludge on the mass ratios between microelements contained in the plant biomass was analyzed as well. The long-term field experiment consisted in application of four levels of fertilization with sewage sludge: 10, 20, 40, and 60 Mg DM·ha-1. A non-fertilized control object (0 Mg DM·ha-1) was used as well. The miscanthus biomass was characterized by higher content of Al and Mn, whereas higher levels of Fe, Co, and Mo were determined in the reed canary grass biomass. As in the case of the element content, the giant miscanthus was characterized by highest level of Al and Mn uptake, while the highest uptake of Fe, Co, and Mo was exhibited by the reed canary grass. In the group of the analyzed microelements, the giant miscanthus showed the highest the recovery of Mo (47%) followed by Mn (39%), Fe (35%), and Co (15%), and the lowest uptake was determined in the case of Al (5%).

In addition to the use of their biomass for the production of renewable energy, energy crops (reed canary grass, giant miscanthus) can be used to recover microelements from municipal sewage sludge. Energy crop biomass can therefore be a potential source of microelements in animal feed. Attention was paid to the mass ratios of Fe:Mn, Fe:Al, Fe:Co, Fe:Mo, Mn:Al, Mn:Co, Mn:Mo, and Co:Mo in the biomass yield of the species used.

Involvement of Dynamic Adjustment of ABA, Proline and Sugar Levels in Rhizomes in Effective Acclimation of Solidago gigantea to Contrasting Weather and Soil Conditions in the Country of Invasion.

Giant goldenrod (Solidago gigantea Aiton) is one of the most invasive plant species occurring in Europe. Since little is known about the molecular mechanisms contributing to its invasiveness, we examined the natural dynamics of the content of rhizome compounds, which can be crucial for plant resistance and adaptation to environmental stress. We focused on rhizomes because they are the main vector of giant goldenrod dispersion in invaded lands. Water-soluble sugars, proline, and abscisic acid (ABA) were quantified in rhizomes, as well as ABA in the rhizosphere from three different but geographically close natural locations in Poland (50°04'11.3″ N, 19°50'40.2″ E) under extreme light, thermal, and soil conditions, in early spring, late summer, and late autumn. The genetic diversity of plants between locations was checked using the random amplified polymorphic DNA (RAPD) markers. Sugar and proline content was assayed spectrophotometrically, and abscisic acid (ABA) with the ELISA immunomethod. It can be assumed that the accumulation of sugars in giant goldenrod rhizomes facilitated the process of plant adaptation to adverse environmental conditions (high temperature and/or water scarcity) caused by extreme weather in summer and autumn. The same was true for high levels of proline and ABA in summer. On the other hand, the lowering of proline and ABA in autumn did not confirm the previous assumptions about their synthesis in rhizomes during the acquisition of frost resistance by giant goldenrod. However, in the location with intensive sunlight and most extreme soil conditions, a constant amount of ABA in rhizomes was noticed as well as its exudation into the rhizosphere. This research indicates that soluble sugars, proline, and ABA alterations in rhizomes can participate in the mechanism of acclimation of S. gigantea to specific soil and meteorological conditions in the country of invasion irrespective of plant genetic variation.

Phytoextraction of heavy metals after application of bottom ash and municipal sewage sludge considering the risk of environmental pollution.

Waste management to reduce the loss of natural resources has become a basis of sustainable development and a circular economy. When using waste, the heavy metal (HM) concentration must be taken into account since HMs can be potentially released to the environment, posing a toxicity threat. The aim of the study was thus to estimate the availability for plants of Cr, Ni, Cu, Zn, Cd, and Pb introduced into the soil with waste. We hypothesized that the prepared waste mixtures containing coal or biomass ash and municipal sewage sludge would reduce the environmental risk compared to the studied waste used separately. The research was conducted during a 6-year field experiment with grasses and legumes. HM concentration in soil, waste, and plant biomass; tolerance index; and uptake of HMs by plants were measured. The ash-sludge mixtures had a more favourable effect on the soil in terms of pHKCl, TOC, total nitrogen, and total exchangeable bases than the waste used separately. This provided beneficial conditions for plant growth and development. Consequently, the ash-sludge mixtures increased the plant yield as compared to ash alone, while the mixture containing the biomass ash also enhanced the yield in relation to the sewage sludge. The study showed that the mixtures allowed for a reduction of environmental risk arising from the HM input with waste to the soil. It was proven that HM availability for plants could be beneficially modified by mixing waste. Combining the coal ash with the sewage sludge is particularly recommended, owing to the unfavourable properties of coal ash for plants. The application of the higher dose of the coal ash-sludge mixture showed a better effect than the lower dose, while the influence of both doses of the biomass ash-sludge mixture was similar. Under the ash-sludge treatment, plants took up more HM than under the ash used separately, and the HM concentration in the obtained biomass did not generally exceed that observed under single wastes. This should reduce the accumulation of HMs in the soil during a long-term use of the waste and facilitates the utilisation of the produced biomass.

Application of ash and municipal sewage sludge as macronutrient sources in sustainable plant biomass production.

Owing to the growing volumes of ash and sewage sludge waste, there is a requirement for theoretical and practical research into the use of these wastes as a source of nutrients. However, there are relatively few studies on the transfer of macronutrients in soil-plant systems amended with ash-sewage sludge mixtures under field conditions. The aim of the study was to determine the effect of bituminous coal ash (AC), biomass ash (AB), and municipal sewage sludge (MSS) on the quantity and quality of a grass-legume mixture. During a 6 year field experiment on a sandy loam soil treated with the wastes, applied as mixtures or separately, the plant yield; N, P, K, Na, Mg, and Ca uptake by plants; macronutrient content and ratios in the plant biomass; and the recovery rate of macronutrients by plants were evaluated. The AB-MSS treatment increased the yield in comparison to that where the wastes were applied separately. The N, P, and Ca contents in the plant biomass and N and P uptake under ash-sludge treatments were in the range observed for the ash and sewage sludge. The AB-MSS co-application resulted in the highest K uptake. The AC-MSS treatment increased K and Mg uptake in relation to AC treatment. When AC or AB was added to the MSS, the Ca uptake increased relative to the MSS treatment. The plant biomass under the AB treatment was optimal for biofuel purposes in terms of the chemical composition. The co-application of AC or AB with MSS resulted in the optimum Ca:Mg ratio for fodder purposes. The recovery rate of the macroelements decreased in the following order: K, N, P, Mg, Na, and Ca. The results support the co-application of solid wastes such as ash and municipal sewage sludge to improve productivity, support the recycling of macronutrients, improve sustainability through the reduction of ash and sewage sludge disposal, and reduce reliance on mineral fertilizer.

Concentration of trace elements in forest soil affected by former timber depot.

Certain parts of global forests show elevated concentrations of trace elements as a result of industry processes, places such as wood depots and plant protection products, which together degrade the forest environment. This paper examines a timber depot that used wood preservatives in World War II located in the Warcino Forest Inspectorate (Poland). It presents monitory findings on the degree to which the upper soil layer in the depot area has been contaminated by wood preservatives. Within two forest divisions, a network of soil extraction points was established, distinguished into three separate categories that demarcate the degrees of vegetation coverage and growth of the common pine. These were Area A (area with a pine stand that is several dozen years-old), Area B (areas with a pine stand that is approximately a dozen years-old) and Area C (areas without a pine stand). The Cu concentration in the surface categories was respectively 141.03/187.54/834.43 mg·kg-1. Above 600% in Cu concentration was noted in category C in comparison to category A. It was found that the content of most elements (B, Cd, Co, Cr, Fe, Mn, Mo, Ni, Pb, Zn) did not exceed the permissible values according to the Regulation of the Minister of the Environment on soil quality standards; however, in the case of Cu, the limit values established for forest and agricultural soils were exceeded, with the highest Cu contents found in the 'C' category. The results obtained confirm that the wood protection chemicals, such as copper sulphate, affected the long-term pollution of forest soils.

A mixture of cellulose production waste with municipal sewage as new material for an ecological management of wastes.

Chemical contaminants present in cellulose production waste and municipal sewage sludge condition the necessity to treat these wastes before they are introduced into the environment. Environmental use of the these wastes appears to be justified owing to the content of organic substances as well as the considerable fertilising value. The aim of the study was to assess chemical composition and ecotoxicity of cellulose production waste and municipal sewage sludge in terms of their eco-friendly application. The assessment was done based on the established soil environment according to regulations in Poland. The research on the chemical composition of the wastes was conducted under laboratory conditions, determining the content of macroelements, microelements and heavy metals with respect to using these wastes in the environment. Phytotoxkit and Microtox tests were performed in order to determine the ecotoxicity of the wastes in the aspect of their environmental use. It was established that, compared to the cellulose production waste, the municipal sewage sludge used in the incubation experiment had a higher content of macroelements and microelements. The content of heavy metals in the waste did not exceed the permissible content that conditions their use in agriculture and reclamation. Based on the results of bioassays it was established that the cellulose production waste was toxic, whereas the combination of cellulose production waste and sewage sludge was found to be of low toxicity to test organisms. The applied wastes and their mixtures were ranked according to phytotoxicity in the following order: cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge. The research results indicate that waste mixtures qualify for environmental use. With respect to the variable chemical composition of cellulose production waste and municipal sewage sludge as well as their ecotoxicity, it was established that each batch intended for environmental use must be subjected to chemical analysis and ecotoxicological tests.

Phytoextraction of heavy metals from municipal sewage sludge by Rosa multiflora and Sida hermaphrodita.

The aim of the study was to evaluate the efficacy of the multiflora rose var. "Jatar" (Rosa multiflora Thunb. ex Murray) and the Virginia fanpetals (Sida hermaphrodita Rusby) to phytoextract heavy metals from municipal sewage sludge. The 6-year field experiment involved four levels of fertilization with sewage sludge at doses of 0, 10, 20, 40, and 60 Mg DM (Dry Mass) sludge ha-1. The increasing doses of sewage sludge were found to significantly increase the yield of multiflora rose and Virginia fanpetals biomass. They also significantly increased the content of heavy metals in these plants. The highest uptake of heavy metals by the multiflora rose and Virginia fanpetals crops was recorded at the fertilization dose of 60 Mg DM ⋅ ha-1. Our investigations show that the Virginia fanpetals was more efficient in the phytoextraction of Cr, Ni, Cu, Zn, and Cd from the sewage sludge than the multiflora rose, due to the greater yields and higher heavy metal uptake by the former plant. In turn, the multiflora rose phytoextracted greater amounts of Pb from the sewage sludge. The analyses indicate that the Virginia fanpetals can be used for phytoremediation (phytoextraction) of heavy metals contained in sewage sludge.

The use of dialdehyde starch derivatives in the phytoremediation of soils contaminated with heavy metals.

Products of the reaction between dialdehyde starch and Y-NH2 compounds (e.g. semicarbazide or hydrazine) are effective ligands for metal ions. The usefulness of these derivatives was tested in the experiment, both in terms of the immobilization of heavy metal ions in soil and the potential application in phytoextraction processes. The experimental model comprised maize and the ions of such metals as: Zn(II), Pb(II), Cu(II), Cd(II), and Ni(II). The amount of maize yield, as well as heavy metal content and uptake by the aboveground parts and roots of maize, were studied during a three-year pot experiment. The results of the study indicate the significant impact of heavy metals on reduced yield and increased heavy metal content in maize. Soil-applied dialdehyde starch derivatives resulted in lower yields, particularly disemicarbazone (DASS), but in heavy metal-contaminated soils they largely limited the negative impact of these metals both on yielding and heavy metal content in plants, particularly dihydrazone (DASH). It was demonstrated that the application of dihydrazone (DASH) to a soil polluted with heavy metals boosted the uptake of Zn, Pb, Cu, and Cd from the soil, hence there is a possibility to use this compound in the phytoextraction of these metals from the soil. Decreased Ni uptake was also determined, hence the possibility of using this compound in the immobilization of this metal. The study showed that dialdehyde starch disemicarbazone was ineffective in the discussed processes.

Remediation of soils on municipal rendering plant territories using Miscanthus × giganteus.

Phytoremediation, as a cost-effective, highly efficient, environmentally friendly, and green approach, gained attention to the removal of metals, including heavy metals, from contaminated soils. The toxic nature of heavy metals can have an adverse effect on human health and the ecosystem, and their removal remains a worldwide problem. Therefore, in this study, a field experiment was carried out to evaluate the potential of Miscanthus × giganteus for the removal of ten microelements and heavy metals (Al, Zn, Fe, Pb, Cd, Co, Cr, Cu, Mn, Ni) from contaminated soil in the territory of a Municipal Waste Rendering Plant. Moreover, the effect of the incorporation of soil improver obtained upon composting biodegradable waste as well as the addition of highly contaminated post-industrial soil on the efficiency of phytoremediation and plant growth was described. The soil improver (SK-8) was applied to the soil at a rate of 200 Mg ha-1 and 400 Mg‧ha-1. Meanwhile, in the last object, 100 Mg‧ha-1 of highly contaminated post-industrial soil was added. Herein, the research was aimed at assessing the possibility of phytoextraction of heavy metals from soils with different physicochemical properties. The results showed that plants cultivated in soil with 400 Mg‧ha-1 of soil improver exhibited the highest yield (approximately 85% mass increase compared to the soil without additives). Furthermore, the application of a single dose of SK-8 (200 Mg ha-1) increased the uptake of Al, Fe, Co, Pb, Mn, Ni, and Cd by Miscanthus × giganteus compared to the soil without additives. Additionally, the performed biotests demonstrated no or low toxicity of the investigated soils affecting the test organisms. However, in all experiments, the phytorecovery of the elements did not exceed 1% of the amount introduced to the soil, which may result from a short cultivation period and large doses of SK-8 or highly contaminated post-industrial soil.

Chemical properties of the coffee grounds and poultry eggshells mixture in terms of soil improver.

Spent coffee grounds (SCG) as well as chicken (CES) or duck eggshells (DES) left over from the artificial hatching technology are proposed as potential soil improver and/or organic-mineral fertiliser components. Therefore, it seems interesting and necessary to evaluate the chemical composition of these wastes and their mixtures in terms of their possible use for that purpose. The study was conducted under the incubation experiment conditions using a mixture of SCG and eggshells (10:1 ratio). Macronutrients, i.e. C, N, S, were determined by the catalytic combustion method, while P, K, Mg, Ca, Na by atomic spectrometry. It was found that SCG were rich in C, N, P, and K, while eggshells in Ca, Mg, Na, and S. However, CES compared to DES were richer in deacidifying components (i.e. Ca, Mg, K). At the same time, the content of macronutrients in eggshells decreased gradually along with the embryo development. For this reason, the mixture of SCG and shells of unembryonated chicken eggs (CES I) had the best chemical and usable proprieties. To conclude, the chemical properties of the mixtures of spent coffee grounds and eggshells indicate their possible application in soil bioengineering.

Polycyclic aromatic hydrocarbon and heavy metal contents in the urban soils in southern Poland.

In this study, we examined how urban and industrial sprawl has affected the concentrations of polycyclic aromatic hydrocarbons (PAHs) and heavy metals (HMs) in the soils of two towns with different levels of urbanisation and industrial development. The concentrations, sources and spatial distributions of PAHs and Zn, Pb and Cd in the soils of the central, residential and green areas of the second largest town in Poland (Krakow) were compared to similar parameters in analogous areas of Zakopane, a small tourist town. The concentration ranges of the PAHs were much higher in the Krakow (337-59,694 µg kg-1) than in the Zakopane (55-1180 µg kg-1) soils. The Krakow soils were also characterised by higher levels of Cd (2.33-4.18 mg kg-1) and Pb (24.2-82.1 mg kg-1) than the Zakopane soils, in which the Cd and Pb concentrations amounted to 0.57-2.29 and 17.8-67.8 mg kg-1, respectively. Positive matrix factorisation (PMF) analysis identified the emission sources of the soil PAHs in both studied towns. In the Krakow soils, the main sources of the PAHs were traffic emissions, petroleum combustion and the steel-industry, while in the Zakopane soils, they were coal and biomass combustion, used for heating houses, followed by vehicle and gasoline emissions. A human health risk assessment, based on the toxic equivalency factors of benzo(a)pyrene (BaPeq), indicated a low level of PAHs contamination in most of the studied areas. Only in one area in central Krakow the BaPeq concentration exceeded the permissible value by nine-fold.

Factors influencing chemical quality of composted poultry waste.

The need for organic recycling is justified in the case of poultry waste because after ensuring hygienization there is a chance of obtaining a compost with substantial fertilizer value. Organic recycling of slaughter waste has its justification in sustainable development and retardation of resources. In the research being described, composting of hydrated poultry slaughterhouse waste with maize straw was carried out. Combinations with fodder yeast and postcellulose lime were also introduced in order to modify chemical and physicochemical properties of the mixtures. The experiment was carried out within 110 days in 1.2 × 1.0 × 0.8 m laboratory reactors. Temperature of the biomass was recorded during composting, and the biomass was actively aerated through a perforated bottom. Composting of substrates selected in such a way caused losses of some elements in gaseous form, an increase in concentration of other elements, and changes in relationships between elements. The ability to select substrates influences compost quality. This ability is determined by chemical indicators. Among other things, compost evaluation based on carbon to nitrogen ratio shows the intensity of the composting process and possible nitrogen losses. The addition of slaughter waste to maize straw reduced the content of individual fractions of carbon in the composts, whereas the addition of postcellulose lime intensified that process. The addition of fodder yeast significantly increased the phosphorus content in the compost. Since iron compounds were used in the processing of poultry carcasses, composts that were based on this material had an elevated iron content. The applied postcellulose lime significantly increased the copper, zinc, chromium, nickel, and lead contents. Proper selection of substrates for composting of hydrated poultry slaughterhouse waste allows to obtain a compost with chemical properties that create favorable conditions for natural application of that compost. Addition of large quantities of postcellulose lime to the composting process leads to obtaining an organic-mineral substratum for cultivation or to obtaining an agent that improves soil properties.

The effect of cellulose production waste and municipal sewage sludge on biomass and heavy metal uptake by a plant mixture.

Environmental management of cellulose production waste and municipal sewage sludge appears to be substantiated due to various physicochemical properties of these wastes. The aim of the conducted research was to determine the effect of cellulose production waste and sewage sludge on yielding and heavy metal uptake by a plant mixture. The research was conducted under field experiment conditions, determining the fertilizer value of these wastes in the environmental aspect. The research was carried out in the years 2013-2016. Species composition of the plant mixture was adjusted to habitat conditions. It was established that, as compared with the cellulose production waste, the municipal sewage sludge used in the experiment had a higher content of macroelements. The content of heavy metals in the studied waste did not exceed the limits that condition their use in agriculture and reclamation. Applying only the cellulose production waste did not significantly decrease the yield of the plants. Municipal sewage sludge showed the highest yield-forming effect. Mixing the above-mentioned wastes and their application to soil had a significant effect on the increase in the plant mixture yield. The waste applied to soil also increased the content of Cr, Cd, Pb, Cu, and Zn in the plant mix. The level of heavy metal content in the plant mix did not exclude this biomass from being used for fodder or reclamation purposes. The cellulose production waste and municipal sewage sludge increased the heavy metal uptake by the plant mixture. The plant biomass extracted heavy metals from the sewage sludge more intensively than from the cellulose production waste. Among the analyzed heavy metals, the highest phytoremediation was recorded for Ni (30%), followed by Cd (20%), Cr (15%), Pb (10%), and the lowest for Cu (9%) and Zn (8%). Application of the cellulose production waste and sewage sludge to soil also increased the content of the studied heavy metals in soil. However, it did not cause deterioration of soil quality standards. Heterogeneity in the chemical composition of the wastes confirms that each batch intended to be used for environmental management should be subjected to chemical control.

The use of reed canary grass and giant miscanthus in the phytoremediation of municipal sewage sludge.

The application of municipal sewage sludge on energy crops is an alternative form of recycling nutrients, food materials, and organic matter from waste. Municipal sewage sludge constitutes a potential source of heavy metals in soil, which can be partially removed by the cultivation of energy crops. The aim of the research was to assess the effect of municipal sewage sludge on the uptake of heavy metals by monocotyledonous energy crops. Sewage sludge was applied at doses of 0, 10, 20, 40, and 60 Mg DM · ha(-1) once, before the sowing of plants. In a 6-year field experiment, the effect of four levels of fertilisation with sewage sludge on the uptake of heavy metals by two species of energy crops, reed canary grass (Phalaris arundinacea L.) of 'Bamse' cultivar and giant miscanthus (Miscanthus × giganteus GREEF et DEU), was analysed. It was established that the increasing doses of sewage sludge had a considerable effect on the increase in biomass yield from the tested plants. Due to the increasing doses of sewage sludge, a significant increase in heavy metals content in the energy crops was recorded. The heavy metal uptake with the miscanthus yield was the highest at a dose of 20 Mg DM · ha(-1), and at a dose of 40 Mg DM · ha(-1) in the case of reed canary grass. Research results indicate that on account of higher yields, higher bioaccumulation, and higher heavy metal uptake, miscanthus can be selected for the remediation of sewage sludge.

The dose-dependent influence of zinc and cadmium contamination of soil on their uptake and glucosinolate content in white cabbage (Brassica oleracea var. capitata f. alba).

The relationship between the ability to accumulate heavy metals (represented by Cd and Zn) and to synthesize bioactive compounds (represented by glucosinolates [GLS]) was investigated in two cabbage cultivars. Plants were grown in the greenhouse of a phytotron under controlled conditions in soils spiked with two different Zn or Cd concentrations. The measurements of Cd and Zn contents in soil and cabbage (leaf) samples were performed by atomic absorption spectroscopy, whereas GLS levels in cabbage were determined by high-performance liquid chromatography. The ranges of metal contents in soil were 80 to 450 mg/kg dry weight for Zn and 0.3 to 30 mg/kg dry weight for Cd, whereas the levels of accumulated Zn and Cd in cabbage amounted to 15 to 130 and 0.02 to 3 mg/kg dry weight, respectively. After initial symptoms of toxicity, during a later stage of growth, the plants exhibited very good tolerance to both metals. Enhanced biosynthesis of GLS was observed in a dose-dependent manner following exposure to the heavy metals. The GLS content in Zn-exposed cabbage rose from 3.2 to 12 µmol/g dry weight, and the corresponding values for Cd-treated plants were 3.5 to 10 µmol/g dry weight. Thus, the increased soil contamination by metals caused greater accumulation in cabbage, as well as stimulation of GLS biosynthesis. The results obtained point to the high phytoremediation and biofumigation potential of white cabbage.