Scene-adaptive spatially channeled imaging Mueller polarimeter.

A spatially adaptive Mueller matrix imaging polarimeter is described, simulated, and demonstrated with preliminary experiments. The system uses a spatial light modulator (SLM) in the polarization state generator (PSG) to create spatial carriers that controlled by the pattern written to the SLM. The polarization state analyzer (PSA) is a commercial division of focal plane imaging polarimeter. The PSG/PSA pair form a 9-channeled partial Mueller matrix polarimeter that measures a 3 × 3 sub-matrix of the Mueller matrix. We demonstrate that adapting the PSG modulation to the spatial frequency structure of the scene can reduce channel crosstalk and improve reconstruction accuracy. Initial experiments are performed that demonstrate the SLM's ability to produce sufficient modulation diversity to create the desired channel structure. Though there are several experimental challenges to obtain accurate Mueller matrix imagery, we demonstrate a system that adapts to the particular scene spatial frequency structure.

Nonlinear focal shift due to the Kerr effect for a Gaussian beam focused by a lens.

When a low-power, monochromatic Gaussian beam is focused by a thin lens in air and the waist of the beam is in the plane of the lens, there is a shift of the focus position if the waist of the beam is much smaller than the size of the lens. The point of maximum intensity relative to the geometrical focal point shifts closer to the lens. We show that for ultra-intense light beams, when the Kerr effect is unavoidable, there is a nonlinear focal shift. The nonlinear focus position shifts closer to the lens for laser powers below the critical power. To avoid the nonlinear focal shift below the critical power, the correct combination of Gaussian beam waist and focal system has to be used in the experimental setup. It will be shown that as the Fresnel number N w associated with the Gaussian beam radius increases, the nonlinear focal shift first increases and then begins to decrease.

Polarimetric measurement of non-depolarizing optical systems.

The use of polarization measurements has become more common in recent years, as it gives more information than pure intensity measurements. Polarimetric components such as fixed or variable retarders and polarizers must be included in optical systems to obtain the polarization parameters required, and in many cases the optical system also includes other components such as relay and/or imaging optical systems. In this work we present a simple and robust method for the polarimetric characterization of non-depolarizing polarization components and other optical elements in the system, which does not require a full polarimeter. Since there is no depolarization, we represent the components as pure retarders with diattenuation and find their parameters (transmittance for the polarization components, angle of orientation of the fast axis, and retardance), from which we can retrieve their Mueller matrix. Our results show that the proposed method is accurate when compared with results obtained with a Mueller matrix dual-rotating retarder polarimeter calibrated using the eigenvalue calibration method, considered in this work as the gold standard, and is comparatively easier than the latter to implement, particularly for imaging polarimeters.

Cholesterol, Amyloid Beta, Fructose, and LPS Influence ROS and ATP Concentrations and the Phagocytic Capacity of HMC3 Human Microglia Cell Line.

Research has found that genes specific to microglia are among the strongest risk factors for Alzheimer's disease (AD) and that microglia are critically involved in the etiology of AD. Thus, microglia are an important therapeutic target for novel approaches to the treatment of AD. High-throughput in vitro models to screen molecules for their effectiveness in reversing the pathogenic, pro-inflammatory microglia phenotype are needed. In this study, we used a multi-stimulant approach to test the usefulness of the human microglia cell 3 (HMC3) cell line, immortalized from a human fetal brain-derived primary microglia culture, in duplicating critical aspects of the dysfunctional microglia phenotype. HMC3 microglia were treated with cholesterol (Chol), amyloid beta oligomers (AßO), lipopolysaccharide (LPS), and fructose individually and in combination. HMC3 microglia demonstrated changes in morphology consistent with activation when treated with the combination of Chol + AßO + fructose + LPS. Multiple treatments increased the cellular content of Chol and cholesteryl esters (CE), but only the combination treatment of Chol + AßO + fructose + LPS increased mitochondrial Chol content. Microglia treated with combinations containing Chol + AßO had lower apolipoprotein E (ApoE) secretion, with the combination of Chol + AßO + fructose + LPS having the strongest effect. Combination treatment with Chol + AßO + fructose + LPS also induced APOE and TNF-α expression, reduced ATP production, increased reactive oxygen species (ROS) concentration, and reduced phagocytosis events. These findings suggest that HMC3 microglia treated with the combination of Chol + AßO + fructose + LPS may be a useful high-throughput screening model amenable to testing on 96-well plates to test potential therapeutics to improve microglial function in the context of AD.

Effects of typical liquid-crystal retarder errors on optimized Stokes polarimeters.

Liquid crystal variable retarders (LCVRs) are often used in Stokes polarimeters as they allow the measurement of different polarization components by applying an electric field that manipulates the induced retardance. However, the optical retardance introduced by these devices is in general not homogenous across the aperture. Another problem with this type of devices is that the fast-axis orientation is not homogenous, and it changes with the applied voltage. For the optimization of polarimeters, in terms of the noise amplification from the intensity measurements to the polarimetric data, the condition number (CN) is often used, but the effects of LCVR spatial variations are not considered. This paper analyzes the impact of errors in LCVRs in a set of optimized Stokes polarimeters simulated by adding errors in the induced retardance and fast-axis orientation. Then, the CN is calculated to observe the effect of these errors on the optimization. We show how errors in the LCVRs lead to different impacts in the polarimetric measurements for different optimized polarimeters, depending on their experimental parameters. Furthermore, we present the propagation error theory to choose the best experimental parameters to reduce the nonideal effects in optimized polarimeters.

Impact of the Cooking Process on Metabolite Profiling of Acanthocereus tetragonus, a Plant Traditionally Consumed in Mexico.

Acanthocereus tetragonus (L.) Hummelinck is used as an alternative food source in some Mexican communities. It has been shown that the young stems of A. tetragonus provide crude protein, fiber, and essential minerals for humans. In this work, we analyzed the phytochemical profile, the total phenolic content (TPC), and the antioxidant activity of cooked and crude samples of A. tetragonus to assess its functional metabolite contribution to humans. The phytochemical profile was analyzed using Ultra-High-Performance Liquid Chromatography coupled to High-Resolution Mass Spectrometry (UHPLC-PDA-HESI-Orbitrap-MS/MS). Under the proposed conditions, 35 metabolites were separated and tentatively identified. Of the separated metabolites, 16 occurred exclusively in cooked samples, 6 in crude samples, and 9 in both crude and cooked samples. Among the detected compounds, carboxylic acids, such as threonic, citric, and malic acids, phenolic acids, and glycosylated flavonoids (luteolin-O-rutinoside) were detected. The TPC and antioxidant activity were analyzed using the Folin-Ciocalteu method and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical inhibition method, respectively. The TPC and antioxidant activity were significantly reduced in the cooked samples. We found that some metabolites remained intact after the cooking process, suggesting that A. tetragonus represents a source of functional metabolites for people who consume this plant species.

Stability of 4 Intravenous Drug Formulations in Prefilled Syringes Stored Frozen for Up to 60 Days.

Purpose: Prefilled drug syringe use may reduce the cost of routine antibiotic drug delivery. Storage of prefilled syringes frozen (-20°C) or refrigerated (4°C-5°C), can optimize the use of robotic syringe filling systems if acceptable stability data is gathered per USP 797 standards. Methods: Four intravenous (IV) drug formulations were prepared from bulk standard solutions and filled into 10 mL syringes using an Intellifill© IV Robot. Formulations were Piperacillin (2.0 g) and Tazobactam (0.25 g) as 2.25 g in 10 mL; Piperacillin (3.0 g) and Tazobactam (0.375 g) as 3.375 g in 10 mL; Cefuroxime as 1.5 g in 11 mL; and Vancomycin as 1.0 g in 10 mL. Concentrations were assayed at "zero time," and after 21, 45, and 60 days frozen. Syringes were warmed to room temperature (RT) by gently rolling in hands. Three syringes of each formulation were assayed by stability-indicating HPLC per USP procedures. Assay results are the average of 5 injections of samples from each syringe upon return to RT and repeated for 3 separate syringes maintained at RT for 24 hours. Results: All formulations were stable out to 60 days frozen. Both of the piperacillin/tazobactam formulations were also stable when kept at refrigerated temperature for 9 days. Conclusion: Piperacillin/Tazobactam formulations can be stored frozen (-20°C) for up to 60 days with no appreciable loss. Cefuroxime and Vancomycin formulations can be stored frozen for up to 60 days. Both Piperacillin/Tazobactam formulations can be refrigerated for up to 9 days. Implementation of larger batch compounding coupled with frozen syringe storage and delivery could result in enhanced uniformity of composition and significant manpower savings.

Calibration and data extraction in a Stokes polarimeter employing three wavelengths simultaneously.

We present a method for calibration and data extraction for a Stokes polarimeter working with three different wavelengths simultaneously. In the Stokes polarimeter considered in this work, we use two liquid crystal variable retarders (LCVRs) combined with a Glan-Thompson linear polarizer. A recently developed fitting calibration procedure is used. We use the same calibration samples and LCVR voltages for all three wavelengths, giving simultaneous measurement and calibration. We compare the performance of the polarimeter, after calibration, using four or six calibration samples in our experiment. To generate the four known calibration beams, we use a linear polarizer oriented at 130° and 30° with respect to the horizontal, a horizontal linear polarizer followed by a half-wave plate (at 632 nm) with its fast axis at 30°, and a horizontal linear polarizer followed by a quarter-wave plate (at 632 nm) with its fast axis at 30°. For calibration with six reference beams, we add two known calibration beams by setting the fast axis of the half- and quarter-wave plates at 130°. Experimental results show good agreement with the expected results, with the fitting calibration procedure giving an approximately 50% reduction in total RMS error with four calibration samples. There is a negligible reduction in the error when six calibration samples are used compared to the case with four samples.

Rough surface scattering using a source able to produce an incident beam with controlled polarization and coherence.

We present a comparison of the first numerical and experimental results for the scattering of light from rough surfaces using a recently developed variable coherence polarimetry source that permits obtaining information on the object without having to scan over incidence or scatter angle. We present, for the first time, we believe, the application of this source to a 1D rough surface and show how to analyze the scattered field to retrieve useful information about the surface. This source uses a liquid-crystal phase modulator to control the polarization as well as the coherence of the beam illuminating the rough surface. Changing the polarization state distribution at the source plane, by controlling the phase distribution on a spatial light modulator, gives a scan of two source spots over the rough surface. The scattered beam is analyzed with a Stokes polarimeter. The Kirchhoff approximation is used to calculate the scattered Stokes vector using the experimental incident Stokes vector and intensity distribution as a source. Good agreement is obtained between the numerical and experimental results, for a simple calculation of the number of intensity maxima obtained as the two first-order source spots are scanned across the sample.

Characterization of retardance spatial variations over the aperture of liquid-crystal variable retarders.

We present a comparison of two experimental methods to measure retardance as a function of applied voltage and as a function of position over the aperture of liquid-crystal variable retarders. These measurements are required for many applications, particularly in polarimetry. One method involves the scan of an unexpanded laser beam over the aperture, and the other uses an expanded beam from a LED and a CCD camera to measure the full aperture with a single measurement. The first method is time consuming, is limited in the measured spatial resolution, and requires more expensive equipment to perform the scan, whereas the second method is low cost, with the spatial resolution of the CCD, and fast, but in principle has variations of the incident beam over the aperture that affect the measured retardance values. The results obtained show good agreement for the average values of retardance for the two methods, but the expanded-beam method shows more noise, particularly close to the voltage values at which the variable-retarder retardance versus voltage curves are unwrapped. These retardance variations can be reduced by smoothing the retardance image, which makes the expanded-beam method an attractive method for polarimetry applications since it gives the complete information in the full aperture of the device with the additional advantages of low cost, simplicity, and being less time consuming.

Rapamycin induces morphological and physiological changes without increase in lipid content in Ustilago maydis.

The evolutionarily conserved serine/threonine kinase TOR recruits different subunits to assemble the Target of Rapamycin Complex 1 (TORC1), which is inhibited by rapamycin and regulates ribosome biogenesis, autophagy, and lipid metabolism by regulating the expression of lipogenic genes. In addition, TORC1 participates in the cell cycle, increasing the length of the G2 phase. In the present work, we investigated the effect of rapamycin on cell growth, cell morphology and neutral lipid metabolism in the phytopathogenic fungus Ustilago maydis. Inhibition of TORC1 by rapamycin induced the formation of septa that separate the nuclei that were formed after mitosis. Regarding neutral lipid metabolism, a higher accumulation of triacylglycerols was not detected, but the cells did contain large lipid bodies, which suggests that small lipid bodies became fused into big lipid droplets. Vacuoles showed a similar behavior as the lipid bodies, and double labeling with Blue-CMAC and BODIPY indicates that vacuoles and lipid bodies were independent organelles. The results suggest that TORC1 has a role in cell morphology, lipid metabolism, and vacuolar physiology in U. maydis.

Impact of frequency-dependent spherical aberration in the focusing of ultrashort pulses.

In this paper, the temporal and spatial intensity pulse distributions are calculated around the focal region of an optical system using a combination of ray tracing and a wave propagation method. We analyze how to measure the width of the intensity pulse distributions to estimate pulse duration and spot size in order to study the impact of the variation of spherical aberration with frequency in a pulse on the intensity distributions. Two experimental techniques used in the laboratory are also modeled: the knife-edge test to measure spatial distribution and the intensity autocorrelation technique to measure the temporal distribution. We use two measuring criteria, the full-width half-maximum (FWHM) and standard deviation (σ), to compare the spatial and temporal intensity distributions of the calculated diffraction patterns and those obtained from the simulated experimental techniques. We show that the FWHM is not a good criterion, since it gives different results in the measured intensity distributions in time and space when they are measured directly from the theoretical modeling and when they are measured from the modeled experimental techniques used in the laboratory. The standard deviation, however, is a consistent criterion, giving the same results for the calculated intensity distributions and the modeled experiments.

Method to calibrate a full-Stokes polarimeter based on variable retarders.

We present a calibration method for a full-Stokes polarimeter. The polarimeter uses two liquid-crystal variable retarders (LCVR) and a linear polarizer to measure the four Stokes parameters. The calibration method proposed in this paper calculates the errors in the experimental setup by fitting the experimental intensity measurements for a set of calibration samples to a theoretical polarimeter with errors. The errors calculated in the method include the axes alignment errors and the errors in the retardance values of both LCVRs. The resulting calibration parameters are verified by measuring the polarization state of a light beam passing through a rotating linear polarizer, a half-wave plate, and a quarter-wave plate and comparing with the predictions for an ideal, error-free polarimeter. It is found that an average reduction in rms error of 55.8% can be obtained with the proposed method.

Secretion of heat shock -60, -70 kD protein, IL-1ß and TNFα levels in serum of a term normal pregnancy and patients with pre-eclampsia development.

The extracellular heat shock proteins (eHsp) family act as molecular chaperones regulating folding, transporting protein and are associated with immune modulation in different physiological and pathological processes. They have been localized in different gestational tissues and their concentration in amniotic fluid and serum has been determined. In the present study, we proposed to determine the concentration of eHsp-60, -70, IL-1ß and TNFα in the serum of pregnant patients with 34 weeks of gestation with and without clinical evidences of preeclampsia (PE). Our results indicate significant increase of these markers in patients with PE with respect to healthy pregnant patients without active labor. Finally, the concentration of eHsp-60 and -70 correlated positively with the hepatic dysfunction markers uric acid, lactate dehydrogenase (LDH), glutamic oxaloacetic transaminase (GOT) glutamic pyruvic transaminase (GPT), and inflammatory IL-1ß and TNFα response. In conclusion, our results demonstrate a strong associated between Hsp and marker of hepatic dysfunction.

Permitted experimental errors for optimized variable-retarder Mueller-matrix polarimeters.

An optimized Mueller-matrix polarimeter is simulated. The polarimeter is optimized by finding the configurations of the polarization state generator and polarization state analyzer that give the minimum condition number. Noise is included in the measurement of the polarimeter intensities, and the eigenvalue calibration procedure is used to reduce the errors in the final Mueller matrix. Controlled errors are introduced to the polarimeter configuration, and the error in the final measured Mueller matrix is calculated as a function of these configuration errors. It is found that the alignment of the retarder axes in the polarimeter is much more important than the use of the ideal, optimized retardance values. In particular, the misalignment of the retarders farthest from the sample is the error source with the highest impact in the precision of the polarimeter.

Experimental limits for eigenvalue calibration in liquid-crystal Mueller-matrix polarimeters.

A numerical study is carried out to find the experimental conditions necessary for the eigenvalue calibration procedure to work correctly in a liquid-crystal variable-retarder-based Mueller-matrix polarimeter. Using the error between the simulated experimental Mueller matrix in a polarimeter with errors and the expected ideal Mueller matrices for four calibration samples, the maximum experimental errors are estimated for a successful eigenvalue calibration. It is found that the retarder axes' orientations have smaller permitted errors than the retardation values.

Microbiological comparison of blood culture and amplification of 16S rDNA methods in combination with DGGE for detection of neonatal sepsis in blood samples.

It is estimated that 15% of all newborns admitted to the neonatal intensive care unit (NICU) for suspected sepsis receive multiple broad-spectrum antibiotics without pathogen identification. The gold standard for bacterial sepsis detection is blood culture, but the sensitivity of this method is very low. Recently, amplification and analysis of the 16S ribosomal DNA (rDNA) bacterial gene in combination with denaturing gradient gel electrophoresis (DGGE) has proven to be a useful approach for identifying bacteria that are difficult to isolate by standard culture methods. The main goal of this study was to compare two methods used to identify bacteria associated with neonatal sepsis: blood culture and broad range 16S rDNA-DGGE. Twenty-two blood samples were obtained from newborns with (n = 15) or without (n = 7) signs and symptoms of sepsis. Blood samples were screened to identify pathogenic bacteria with two different methods: (1) bacteriological culture and (2) amplification of the variable V3 region of 16S rDNA-DGGE. Blood culture analysis was positive in 40%, whereas 16S rDNA-DGGE was positive in 87% of neonatal sepsis cases. All 16S rDNA-DGGE positive samples were associated with some other signs of neonatal sepsis. CONCLUSION: Our study shows that the molecular approach with 16S rDNA-DGGE identifies twofold more pathogenic bacteria than bacteriological culture, including complex bacterial communities associated with the development of bacterial sepsis in neonates. What is Known: • Neonatal sepsis affects 2.3% of birth in the NICU with a high mortality risk. • Evidence supports the use of molecular methods as an alternative to blood culture for identification of bacterial associated neonatal sepsis. What is New: • The DGGE gel is a good methodological approach for the identification of bacterial in neonatal blood samples. • This study describes the pattern of electrophoretic mobility obtained by DGGE gels and allows to determine the type of bacteria associated in the development of neonatal sepsis.

Structural and kinetics characterization of the F1F0-ATP synthase dimer. New repercussion of monomer-monomer contact.

Ustilago maydis is an aerobic basidiomycete that fully depends on oxidative phosphorylation for its supply of ATP, pointing to mitochondria as a key player in the energy metabolism of this organism. Mitochondrial F1F0-ATP synthase occurs in supramolecular structures. In this work, we isolated the monomer (640kDa) and the dimer (1280kDa) and characterized their subunit composition and kinetics of ATP hydrolysis. Mass spectrometry revealed that dimerizing subunits e and g were present in the dimer but not in the monomer. Analysis of the ATPase activity showed that both oligomers had Michaelis-Menten kinetics, but the dimer was 7 times more active than the monomer, while affinities were similar. The dimer was more sensitive to oligomycin inhibition, with a Ki of 24nM, while the monomer had a Ki of 169nM. The results suggest that the interphase between the monomers in the dimer state affects the catalytic efficiency of the enzyme and its sensitivity to inhibitors.

Optical concatenation of a large number of beads with a single-beam optical tweezer.

Optical tweezers consist of the spatial confinement of microscopic dielectric particles by the action of forces produced by the change in momentum of the photons of a highly focused laser beam that are deviated by the particle. In experiments that use a single laser beam, it is common to capture not only one but a few particles in the optical trap. However, to our knowledge, the formation of a long chain of beads optically confined with a single laser beam has never been reported. In this work, up to 73 silica spheres immersed in water are seen concatenated along the propagation direction of a 976-nm wavelength Gaussian laser of 300 mW of power. This long chain of beads is obtained when the laser is focused through an oil-immersion DIN microscope objective with 100× magnification and a numerical aperture of 1.25. When performing the same experiment using an infinity-corrected UplanFLN 100× objective with a numerical aperture of 1.3, the maximum number of concatenated beads is only 14. Our results suggest that the mechanisms responsible for the observed phenomena involve successive refocusing of the laser beam by each trapped sphere, optically induced dipole coupling (commonly referred to as optical binding), and aberrations generated by the DIN microscope objective.

Calibration and data extraction in nonoptimized Mueller matrix polarimeters.

We present a method for calibration and data extraction for a nonoptimized Mueller matrix polarimeter. The advantage of this type of method is a reduction in measurement time for multiwavelength systems or in systems with slow response times. The calibration process requires the measurement of four known polarization devices. Here we use free-space transmission, a horizontal and a vertical linear polarizer, and a quarter-wave retarder with its fast axis at 30° to the horizontal. Experimental measurements of rotating quarter-wave and half-wave retarders show that accurate results can be obtained with the proposed method.