Immune and inflammation-related gene polymorphisms and susceptibility to tuberculosis in Southern Xinjiang population: A case-control analysis.

Genetic and immune factors play an important role in tuberculosis. Under different ethnicities and genetic backgrounds, different immune and inflammation-related gene polymorphisms may confer different susceptibility to tuberculosis. This study investigated the relationship between immune and inflammation-related gene polymorphism and susceptibility to tuberculosis in Xinjiang Uyghur population, China. In this case-control study, we enrolled 507 pulmonary tuberculosis patients and 454 healthy controls from Southern Xinjiang. single nucleotide polymorphism (SNP) genotyping was performed. The 12 SNPs of nine immune and inflammation-related genes (including TNF rs361525, IL6 rs2066992 and rs1524107, IL17A rs3748067, IL17F rs763780, VDR rs731236, rs2228570 and rs1544410, IFNGR1 rs1327474, P2RX7 rs3751143, CTAGE1 rs4331426 and Toll-like receptor 4 (TLR4) rs4986790) and their relationship with tuberculosis were evaluated. The T allele and TT genotype of IL-6 rs2066992 and rs1524107 increased the risk of active tuberculosis. The C allele of IFNGR1 rs1327474 was related to the reduced risk of tuberculosis in the Xinjiang Uyghur population. The G allele and AG/GG genotypes of TLR4 rs4986790 were associated with an increased risk of tuberculosis (p < .05). Furthermore, haplotype analysis found that the haplotype TT of interleukin (IL)-6 was a risk factor, whereas the CG type was a protective factor for active tuberculosis in the Xinjiang Uyghur population. There were three immune and inflammation-related genes (IL-6, IFNGR1 and TLR4) and a total of four SNPs (rs2066992, rs1524107, rs1327474 and rs4986790) related to the susceptibility of the Uyghur population to tuberculosis. Our findings may provide evidence for further understanding the mechanism of tuberculosis susceptibility in the Xinjiang Uyghur population.

Study on the Strength and Hydration Behavior of Sulfate-Resistant Cement in High Geothermal Environment.

The hydration process and compressive strength and flexural strength development of sulphate-resistant Portland cement (SRPC) curing at 20 °C, 40 °C, 50 °C, and 60 °C were studied. In addition, MIP, XRD, SEM, and a thermodynamic simulation (using Gibbs Energy Minimization Software (GEMS)) were used to study the pore structure, the types, contents, and transformations of hydration products, and the changes in the internal micro-morphology. The results indicate that, compared with normal-temperature curing (20 °C), the early compressive strength (1, 3, and 7 d) of SRPC cured at 40~60 °C increased by 10.1~57.4%, and the flexural strength increased by 1.8~21.3%. However, high-temperature curing was unfavorable for the development of compressive strength and flexural strength in the later period (28~90 d), as they were reduced by 1.5~14.6% and 1.1~25.5%, respectively. With the increase in the curing temperature and curing age, the internal pores of the SRPC changed from small pores to large pores, and the number of harmful pores (>50 nm) increased significantly. In addition, the pore structure was further coarsened after curing at 60 °C for 90 d, and the number of multiple harmful pores (>200 nm) increased by 17.9%. High-temperature curing had no effect on the types of hydration products of the SRPC but accelerated the formation rate of hydration products. The production of the hydration products C-S-H increased by 13.5%, 18.6%, and 22.8% after curing at 40, 50, and 60 °C for 3 d, respectively. The stability of ettringite (AFt) reduced under high-temperature curing, and its diffraction peak was not observed in the XRD patterns. When the curing temperature was higher than 50 °C, AFt began to transform into monosulfate, which consumed more tricalcium aluminate hydrate and inhibited the formation of "delayed ettringite". Under high-temperature curing, the compactness of the internal microstructure of the SRPC decreased, and the distribution of hydration products was not uniform, which affected the growth in its strength during the later period.

Novel mesoporous silica nanocarriers containing gold; a rapid diagnostic tool for tuberculosis.

Tuberculosis (TB) is major health concern and reason of deaths from decades to current date. Even though with a lot of advancements, diagnostic techniques, and discovery of standard antibiotics TB remains crucial challenge and can create worst scenario for human health in near future. Nanoparticles play emerging role in diagnosis and treatment of TB. In this study, we developed mesoporous silica nanoparticles containing gold (MSNs@GNPs) for rapid diagnosis and treatment of TB. The physicochemical characterization revealed effective surface morphology and particles diameter, that is applicable for in vitro applications. The in vitro antimicrobial analysis revealed that the designed MSNs@GNPs has retained significantly lower minimal inhibitory concentration (MIC) values and can effectively demolish mycobacterium tuberculosis (Mtb). Furthermore, the diagnosis efficiency of the MSNs@GNPs was evaluated by calorimetric analysis. Which demonstrates that MSNs@GNPs can be used for rapid diagnosis of the tuberculosis when applied on in vitro culture of the Mtb. The current study needs further verification on human's clinical samples from tuberculosis patients. However, MSNs@GNPs can be a versatile clinical approach for the rapid diagnosis and clinical treatment of the tuberculosis.

Study on Mechanical Properties of Basalt Fiber Shotcrete in High Geothermal Environment.

With the development of infrastructure, there are growing numbers of high geothermal environments, which, therefore, form a serious threat to tunnel structures. However, research on the changes in mechanical properties of shotcrete under high temperatures and humid environments are insufficient. In this paper, the combination of various temperatures (20 °C/40 °C/60 °C) and 55% relative humidity is used to simulate the effect of environment on the strength and stress-strain curve of basalt fiber reinforced shotcrete. Moreover, a constitutive model of shotcrete considering the effect of fiber content and temperature is established. The results show that the early mechanical properties of BFRS are improved with the increase in curing temperature, while the compressive strength at a later age decreases slightly. The 1-day and 7-day compressive strength of shotcrete at 40 °C and 60 °C increased by 10.5%, 41.1% and 24.1%, 66.8%, respectively. The addition of basalt fiber can reduce the loss of later strength, especially for flexural strength, with a increase rate of 11.9% to 39.5%. In addition, the brittleness of shotcrete increases during high temperature curing, so more transverse cracks are observed in the failure mode, and the peak stress and peak strain decrease. The addition of basalt fiber can improve the ductility and plasticity of shotcrete and increase the peak strain of shotcrete. The constitutive model is in good agreement with the experimental results.

Autosomal InDel polymorphisms for population genetic structure and differentiation analysis of Chinese Kazak ethnic group.

In the present study, we assessed the genetic diversities of the Chinese Kazak ethnic group on the basis of 30 well-chosen autosomal insertion and deletion loci and explored the genetic relationships between Kazak and 23 reference groups. We detected the level of the expected heterozygosity ranging from 0.3605 at HLD39 locus to 0.5000 at HLD136 locus and the observed heterozygosity ranging from 0.3548 at HLD39 locus to 0.5283 at HLD136 locus. The combined power of discrimination and the combined power of exclusion for all 30 loci in the studied Kazak group were 0.999999999999128 and 0.9945, respectively. The dataset generated in this study indicated the panel of 30 InDels was highly efficient in forensic individual identifcation but may not have enough power in paternity cases. The results of the interpopulation differentiations, PCA plots, phylogenetic trees and STRUCTURE analyses showed a close genetic affiliation between the Kazak and Uigur group.