Functional Characterization of the MeSSIII-1 Gene and Its Promoter from Cassava.

Soluble starch synthases (SSs) play important roles in the synthesis of cassava starch. However, the expression characteristics of the cassava SSs genes have not been elucidated. In this study, the MeSSIII-1 gene and its promoter, from SC8 cassava cultivars, were respectively isolated by PCR amplification. MeSSIII-1 protein was localized to the chloroplasts. qRT-PCR analysis revealed that the MeSSIII-1 gene was expressed in almost all tissues tested, and the expression in mature leaves was 18.9 times more than that in tuber roots. MeSSIII-1 expression was induced by methyljasmonate (MeJA), abscisic acid (ABA), and ethylene (ET) hormones in cassava. MeSSIII-1 expression patterns were further confirmed in proMeSSIII-1 transgenic cassava. The promoter deletion analysis showed that the -264 bp to -1 bp MeSSIII-1 promoter has basal activity. The range from -1228 bp to -987 bp and -488 bp to -264 bp significantly enhance promoter activity. The regions from -987 bp to -747 bp and -747 bp to -488 bp have repressive activity. These findings will provide an important reference for research on the potential function and transcriptional regulation mechanisms of the MeSSIII-1 gene and for further in-depth exploration of the regulatory network of its internal functional elements.

[Clinical application of plasma exchange combined with continuous veno-venous hemofiltration dialysis in children with refractory Kawasaki disease shock syndrome]. / 血浆置换联合连续性静脉-é™è„‰è¡€æ¶²æ»¤è¿‡é€æžæ²»ç–—åœ¨å„¿ç«¥å·å´Žç— ä¼‘å ‹ç»¼åˆå¾ä¸­çš„ä¸´åºŠåº”ç”¨.

OBJECTIVES: To study the role of plasma exchange combined with continuous blood purification in the treatment of refractory Kawasaki disease shock syndrome (KDSS). METHODS: A total of 35 children with KDSS who were hospitalized in the Department of Pediatric Intensive Care Unit, Hunan Children's Hospital, from January 2019 to August 2022 were included as subjects. According to whether plasma exchange combined with continuous veno-venous hemofiltration dialysis was performed, they were divided into a purification group with 12 patients and a conventional group with 23 patients. The two groups were compared in terms of clinical data, laboratory markers, and prognosis. RESULTS: Compared with the conventional group, the purification group had significantly shorter time to recovery from shock and length of hospital stay in the pediatric intensive care unit, as well as a significantly lower number of organs involved during the course of the disease (P<0.05). After treatment, the purification group had significant reductions in the levels of interleukin-6, tumor necrosis factor-α, heparin-binding protein, and brain natriuretic peptide (P<0.05), while the conventional group had significant increases in these indices after treatment (P<0.05). After treatment, the children in the purification group tended to have reductions in stroke volume variation, thoracic fluid content, and systemic vascular resistance and an increase in cardiac output over the time of treatment. CONCLUSIONS: Plasma exchange combined with continuous veno-venous hemofiltration dialysis for the treatment of KDSS can alleviate inflammation, maintain fluid balance inside and outside blood vessels, and shorten the course of disease, the duration of shock and the length of hospital stay in the pediatric intensive care unit.

MiR-125b enhances autophagic flux to improve septic cardiomyopathy via targeting STAT3/HMGB1.

We explore the role of miR-125b in septic cardiomyopathy, focusing on miR-125b/STAT3/HMGB1 axis. CLP mouse model and LPS-stimulated primary rat cardiomyocytes (CMs) and H9C2 cell were used as in vivo and in vitro models of septic cardiomyopathy, respectively. qRT-PCR and western blot were performed to measure expression levels of miR-125b, STAT3, HMGB1, and autophagy-related proteins. MTT assay was employed to examine LPS toxicity. Dual luciferase activity assay and CHIP were performed to validate interactions of miR-125b/STAT3 and STAT3/HMGB1 promoter. Immunostaining was used to assess the level of autophagic flux. ROS level was measured by fluorescence assay. Heart functions were examined via intracoronary Doppler ultrasound. miR-125b was diminished while STAT3 and HMGB1 were elevated in the heart tissue following CLP surgery and in LPS-treated H9C2 cells. LPS treatment up-regulated ROS generation and suppressed autophagic flux. Overexpression of miR-125b mimics or knockdown of STAT3 or HMGB1 alleviated LPS-induced hindrance of autophagic flux and ROS production. miR-125b directly targeted STAT3 mRNA and STAT3 bound with HMGB1 promoter. Overexpression of miR-125b mitigated myocardial dysfunction induced by CLP in vivo. Hyperactivation of STAT3/HMGB1 caused by reduced miR-125b contributes to ROS generation and the hindrance of autophagic flux during septic cardiomyopathy, leading to myocardial dysfunction.

[Application value of metagenomic next-generation sequencing for pathogen detection in childhood agranulocytosis with fever]. / ç— åŽŸä½“å®åŸºå› ç»„äºŒä»£æµ‹åºåœ¨å„¿ç«¥ç²’ç»†èƒžç¼ºä¹ä¼´å‘çƒ­ä¸­çš„åº”ç”¨ä»·å€¼.

OBJECTIVES: To study the application value of metagenomic next-generation sequencing (mNGS) for pathogen detection in childhood agranulocytosis with fever. METHODS: A retrospective analysis was performed on the mNGS results of pathogen detection of 116 children with agranulocytosis with fever who were treated from January 2020 to December 2021. Among these children, 38 children with negative mNGS results were enrolled as the negative group, and 78 children with positive results were divided into a bacteria group (n=22), a fungal group (n=23), and a viral group (n=31). Clinical data were compared between groups. RESULTS: For the 116 children with agranulocytosis and fever, the median age was 8 years at diagnosis, the median turnaround time of mNGS results was 2 days, and the positive rate of mNGS testing was 67.2% (78/116). Compared with the negative group, the bacterial group had a higher procalcitonin level (P<0.05), the fungal group had higher level of C-reactive protein and positive rate of (1,3)-ß-D glucan test/galactomannan test (P<0.05), and the fungal group had a longer duration of fever (P<0.05). Among the 22 positive microbial culture specimens, 9 (41%) were consistent with the mNGS results. Among the 17 positive blood culture specimens, 8 (47%) were consistent with the mNGS results. Treatment was adjusted for 28 children (36%) with the mNGS results, among whom 26 were cured and discharged. CONCLUSIONS: The mNGS technique has a shorter turnaround time and a higher sensitivity for pathogen detection and can provide evidence for the pathogenic diagnosis of children with agranulocytosis and fever.

[Vasovagal syncope or postural orthostatic tachycardia syndrome in children with neurological symptoms at disease onset: a clinical analysis of 88 cases].

OBJECTIVE: To study the clinical features of vasovagal syncope (VVS) and postural orthostatic tachycardia syndrome (POTS) in children with neurological symptoms at disease onset. METHODS: A retrospective analysis was performed on the medical data of 88 children with the initial symptoms of the nervous system, such as transient loss of consciousness, dizziness, headache, and convulsion, who were finally diagnosed with VVS or POTS. RESULTS: Of the 88 children, there were 35 boys (40%) and 53 girls (60%), with an age of 4-15 years. The peak age of onset was between 10 and 13 years. All the children had the initial symptoms of transient loss of consciousness, dizziness, headache, and convulsion. Nervous system diseases were excluded by electroencephalography, cerebrospinal fluid examination, and cranial MRI. Of the 88 children, 53 (60%) were confirmed with VVS, and 35 (40%) with POTS, according to the results of head-up tilt test (HUTT). Five children with the initial symptom of transient loss of consciousness were misdiagnosed with epilepsy. Predisposing factors were determined for 59 children (67%), and prolonged standing was the most common factor, followed by change in body position and strenuous exercise. Premonitory symptoms were observed in 66 children (75%), among which chest discomfort was the most common symptom, followed by gastrointestinal symptoms (nausea, vomiting, and abdominal pain) and pale complexion. All 88 children received health education and exercise for autonomic nerve function, among whom 53 children with VVS were given oral rehydration salts and 35 children with POTS were given oral rehydration salts and metoprolol. All 88 children were followed up for 18 months, and the response rates to the above treatment at 3, 6, 12, and 18 months of follow-up were 87%, 93%, 93%, and 90% respectively. CONCLUSIONS: In addition to nervous system diseases, functional cardiovascular diseases including VVS and POTS should be considered for children with the initial symptoms of transient loss of consciousness, dizziness, headache, and convulsion. HUTT can be used to make a confirmed diagnosis, and the early treatment can achieve a good outcome.

Ground observation result of an OH radical hyper-resolution spectrometer for the middle and upper atmosphere.

OH radicals in the upper and middle atmosphere are important oxidants and play an important role in atmospheric photochemistry. A hyper-resolution spectrometer based on 308 nm glow was developed for obtaining OH radical concentration data in the upper and middle atmosphere. In order to verify the performance of the OH radical hyper-resolution spectrometer, several comprehensive ground experiments were carried out in this paper. The spectrometer observes OH radicals produced by a photochemistry reactor chamber to verify the detection ability of the instrument for characteristic signals. A solar observation experiment is used to evaluate the hyper-resolution spectroscopic ability of the spectrometer and the on-orbit field-of-slice-view function. In order to evaluate the detection ability of weak atmospheric background radiation, the experimental study of solar scattering light observation was carried out. The experimental results show that the spectrometer has the characteristics of ultrahigh spectral resolution (0.0086 nm), high sensitivity, and high signal-to-noise ratio. The ground observation results are consistent with the theoretical simulation values.

Polarization sensitivity error analysis and measurement of a greenhouse gas monitoring instrument.

Greenhouse gas monitoring instruments (GMI) are spatial heterodyne spectroscopy (SHS) sensors that monitor greenhouse gases (GHG) from space. Due to several kinds of polarization-sensitive optical elements in GMIs, to some extent, the instrument becomes a polarization-sensitive sensor. Its polarization sensitivity will reduce the radiometric accuracy and spectral inversion accuracy of GHG column concentration. Theoretical radiation response models for analyzing the polarization sensitivity of a GMI, which is mainly affected by a scanning mirror beam splitter and diffraction gratings, are presented in this paper. Based on these models and the polarization performance testing, the theoretical and experimental results of the main spectral band of a GMI, covering the wavelength range of 1.568-1.583 µm for carbon dioxide (CO2) detection, have been given. The result shows that the linear polarization sensitivity is less than 0.65% and 1.32% in the nadir (45°, 0°) and in the oblique view direction (45±20°, ±31°), respectively, and that it meets the qualification requirement for an absolute radiometric calibration accuracy better than 5%. The absolute radiometric calibration accuracy directly affects the accuracy of GHG concentration retrieval.

Alignment error analysis of detector array for spatial heterodyne spectrometer.

Spatial heterodyne spectroscopy (SHS) is a new spatial interference spectroscopy which can achieve high spectral resolution. The alignment error of the detector array can lead to a significant influence with the spectral resolution of a SHS system. Theoretical models for analyzing the alignment errors which are divided into three kinds are presented in this paper. Based on these models, the tolerance angle of these errors has been given, respectively. The result of simulation experiments shows that when the angle of slope error, tilt error, and rotation error are less than 1.21°, 1.21°, 0.066° respectively, the alignment reaches an acceptable level.

Data processing error analysis based on Doppler asymmetric spatial heterodyne measurement.

The Doppler asymmetric spatial heterodyne (DASH) interferometer provides the capability to retrieve wind speed in the upper atmosphere. The data processing leads to a significant retrieving error with the development of wind precision. The influence of window parameters on the isolated interferogram is analyzed theoretically. Based on the derivation, the system is established, and the interferogram with a small shift is sampled. The phase and wind speed are calculated under various window types and window width values. We conclude that, by choosing the Nuttall window with a small width, the wind retrieving error can be minimized.

The Research of Flatfielding Correction Method for Spatial Heterodyne Spectrometer at Systematic Level.

It can't satisfy the requirement of correction for response non-uniformity at systematic levelif only if the array detector for spatial heterodyne spectrometer is corrected. Traditional methods, such as irradiation with uniform source and column-flat-fielding, are not suitable for spatial heterodyne spectrometer. The article expounds convection arm-blocking method for spatial heterodyne spectrometer briefly at first. This method leads to kinds of mismatches including pixel and sub-pixel level shift and rotation in a single arm data after gluing gratings. The effect of registration accuracy of flatfielding coefficients has been analyzed for the experimental breadboard. The result shows that the registration accuracy of flatfielding coefficients needs to be better than 0.1 pixel for the breadboard. The shift at pixel level is calculated by solving the rotational degree by using logarithm-polar coordinate and phase correlation method for the requirement of registration. The shift at sub-pixel level is estimated with DFT based on matrix multiplication. The flow path of flatfielding method at systematic level is concluded. The integral condition of interferometer after actual gluing is modulated by adjusting the positions of gratings slightly. The flatfielding flow path is applied to the data acquired from the modulated interferometer after gluing. Then, the result is compared with the spectrum after the correction with totally matched signal arm data. The final result shows that the spectral deviation is 0.6% between the two spectra compared with the spectral deviation of 4.1% without correction. The accuracy of recovered spectrum after correction has been improved markedly. This can be the foundation for the follow data processing.

[Error Correction of Spectral Calibration for Hyper-Spectral Atmosphere CO2 Monitoring Instrument].

The detection of hyper-spectral atmosphere CO2 needs remote sensor to be characterized and calibrated precisely while spectral calibration is the most basic work. The high uncertainty of wavelength calibration coefficient is a big problem as to the traditional laboratory calibration methods. In order to solve this problem, the research of error correction of spectral calibration based on gas absorption principle is carried out. The method is in accordance with the using conditions of instrument and it improves the practicability of the calibration coefficient. First, theoretical spectrum and error components are simulated by using radiative transfer. Then, the experiment of atmosphere CO2 absorption spectrum measurement is performed based on the atmosphere environmental simulation calibration house. Last, spectral error is corrected and optimized with LM algorithm. The result of spectral calibration of error correction shows that the mean value of spectral error deviation decreases from 0.03 cm-1 before correction to 0.008 cm-1 after correction, and systematic and mutable errors are removed. The spectral calibration precision on the ground is improved significantly, which lays the foundation for the subsequent greenhouse gas retrieval.

[Study on Asymmetric Spatial Heterodyne Spectroscopy].

Restrictive relationship exists between spectral resolution, spectral range and number of pixels of traditional Spatial Heterodyne Spectroscopy (SHS). The main difference between Asymmetric Spatial Heterodyne Spectroscopy (ASHS) and SHS accelerates the space of one grating from the beamsplitter. It greatly increases spectral resolution while system parameters remain unchanged. First of all, this paper elaborates the fundamentals of the ASHS, the derived formulas of the system parameters and theoretical relationship between grating offset and the spectral resolution increases. As an important parameter of the ASHS, offset is restricted by the pixel number of short double side interferogram and the spectral resolution requirements. According to the experimental breadboard parameters of laboratory, the selection principle and the results of the offset are presented. In the case of the same device parameters, two types of theoretical performance parameters are calculated. The simulation is carried out. The results show that two of them have the same spectral range, but the ASHS has a higher spectral resolution. The relationship between resolution and offset increased consistent with theoretical calculation. Finally the ASHS breadboard is calibrated with the monochromatic light scanning method. The derived spectral range and resolution are in good agreement with the theoretical value.

[The Research of Spatial Heterodyne Raman Spectroscopy with Standoff Detection].

Spatial heterodyne Raman spectroscopy (SHRS) is a new type of Raman spectroscopic detection technique with characteristics of high optical throughout, high spectral resolution, and no moving parts. SHRS is very suitable for the planetary exploration missions, which can be used to the analysis of minerals and find the biomarkers maybe exist on the surface of planetary. The authors have applied the technique to the standoff Raman spectroscopic detection, analyzed the main characteristics, including spectral resolution, bandpass and signal to noise (SNR), of standoff SHRS and proved it through experiments. The basic theory of standoff SHRS has been described briefly while a breadboard has been designed, built and calibrated. On the basis, the Raman spectra of some inorganic solids, organic liquids and some natural minerals have been achieved at a distance of 10 m, the SNR of the breadboard has been estimated. Due to the poor adjustment and the defects of the optical elements, the breadboard is far away from an ideal system. But the results show that the SNR is better than 5 for most of the main Raman peaks of the samples, which can meet the basic requirement of clear positive detection of typical Raman peaks and the feasibility of standoff SHRS has been proved. SHRS can overcome the main defects of dispersive grating Raman spectrometers and Fourier transform Raman spectrometers and it has a great application prospect on the detection and analysis of the planetary surface. The work of the authors can prove the potentiality of SHRS on standoff detection and can provide reference for the engineering realization of standoff SHRS.

[Study on the Spectral Characteristics of the Narrow-Band Filter in SHS].

The spectral response of spatial heterodyne spectroscopy (SHS) is determined by the spectrum property of narrow-band filter. As discussed in previous studies, the symmetric heterodyned interferogram of high frequency waves modulated by SHS and lack of sample lead to spectral confusion, which is associated with the true and ghost spectra. Because of the deviation from theoretical index of narrow-band filter in the process of coating, the boarded spectral response and middle wave shift are presented, and conditions in the theoretical Littrow wavelength made the effective wavelength range of SHS reduced. According to the measured curve of filter, a new wavenumber of zero spatial frequency can be reset by tunable laser, and it is easy for SHS to improve the spectral aliasing distortion. The results show that it is utilized to the maximum extent of the effective bandwidth by adjusting the grating angle of rotation to change the Littrow wavelength of the basic frequency, and the spectral region increased to 14.9 nm from original 12.9 nm.

[Thermal effect on optical properties of spatial heterodyne spectroscopy].

To study the thermal effort on optical properties of spatial heterodyne spectroscopy (SHS), the relation between the change in temperature and the degradation of optical properties was analyzed, including collimator lens, imaging lens and interferometer unit. The theoretical model was verified and corrected by simulation and thermal-optical test, which was established to describe the relation between spectral-line shift and groove density change of gratings. The results show that spectrum measurements are not affected by small temperature changes in the instrument since the primary effects. are a slight defocus of the focal plane array in collimating lens and re-imaging lens under the temperature from 15 to 25 °C. Spectral-line shift obtained by the thermal optical analysis is well coincident with the ones calculated by thermal optical experiment because of the temperature chan- ges of interferometer unit. The Littrow wavelength accuracy is better than 2.3 nm unless the temperature change is less than 2.5 °C under the interferometer unit by use of the material BK7.

[Impact of continuous blood purification on T cell subsets in children with severe sepsis].

OBJECTIVE: To investigate the impact of continuous blood purification (CBP) on T-cell subsets and prognosis in children with severe sepsis. METHODS: A total of 42 children with severe sepsis were randomly divided into a control group (n=22) and a CBP group (n=20). The patients in the control group received conventional treatment, while those in the CBP group underwent continuous veno-venous hemofiltration daily 12-24 hours for 3 days besides conventional treatment. Changes in clinical variables and in peripheral blood regulatory T cell subsets were assessed 3 and 7 days after treatment. RESULTS: The pediatric intensive care unit length of stay and duration of mechanical ventilation were significantly shortened and the 28-day mortality rate was significantly lower in the CPB treatment group as compared with the control group (P<0.05). In the CBP treatment group, the percentage of CD3(+), CD4(+), CD8(+) T cell populations and PCIS scores were significantly higher at 3 and 7 days after treatment than before treatment (P<0.05). At 7 days after treatment, the percentage of CD3(+), CD4(+), CD8(+) T cell populations, CD4(+)/CD8(+) ratio and PCIS scores were significantly higher in the CBP group than in the control group (P<0.05). CONCLUSIONS: The CBP treatment may counteract the suppression of immune function and thus improve prognosis in children with severe sepsis.

Serum procalcitonin as a marker of neonatal intrahepatic cholestasis caused by citrin deficiency (NICCD).

BACKGROUND: Neonatal Intrahepatic Cholestasis (NICCD), as the early-age stage of Citrin deficiency involving liver dysfunction, lacks efficient diagnostic markers. Procalcitonin (PCT) has been identified as a biomarker for infection as well as various organ damage. This study aimed to explore the potential of PCT as a biomarker for NICCD. METHODS: In a single-center retrospective case-control study. Serum PCT concentrations before and after treatment of 120 NICCD patients, as the study group, were compared to the same number of cholestatic hepatitis patients, as the control group. The potential value of PCT to discriminate NICCD from control disease was further explored using Receiver Operating Characteristic (ROC) curve analysis and compared to those of other inflammatory markers. RESULTS: There was a significantly higher level of PCT in NICCD patients than in the control group. PCT concentrations were only weakly correlated with neutrophil counts and CRP levels (p ˂ 0.05). At a cut-off value of 0.495 ng/mL, PCT exhibited a significantly higher diagnostic value compared to other inflammatory markers for discriminating NICCD from the control, with a sensitivity of 90.8 % and specificity of 98.3 %. CONCLUSION: PCT might be used as an initial biomarker to discriminate children with NICCD from another hepatitis disease.

[Changes in T cell subsets in children with sepsis, and their clinical significance].

OBJECTIVE: To observe changes in T cell subsets in children with sepsis and their prognosis, and to investigate the clinical significance of these changes in the occurrence and development of sepsis. METHODS: Fifty children with severe sepsis and 150 children with general sepsis were enrolled as subjects, and 50 age-matched healthy children were included as controls. The percentages of CD3(+), CD4(+) and CD8(+) T cells in peripheral blood and CD4(+)/CD8(+) ratio were measured by flow cytometry. The pediatric critical illness score (PCIS) was calculated within 24 hours of admission. RESULTS: The children with severe sepsis showed significantly lower percentages of CD3(+), CD4(+) and CD8(+) T cells CD4(+)/CD8(+) ratio and PCIS than the controls and children with general sepsis (P<0.01). Among the 200 cases of sepsis, the percentages of CD3(+), CD4(+) and CD8(+) T cells, CD4(+)/CD8(+) ratio and PCIS were significantly lower in the cured group than in the deceased group. CONCLUSIONS: Children with sepsis have different degrees of cellular immunosuppression, and the degree of cellular immunosuppression is significantly correlated with the severity of the disease. Detection of T cell subsets in peripheral blood is of great significance for evaluating immune function and judging disease severity in children with sepsis.

Clinical, histological and direct immunofluorescence features in oral mucosal patches striae diseases with malignant potential.

Background/purpose: Oral mucosal patches striae diseases (OMPSD) represent an important category of oral mucosal disease, most of which may have malignant potential (OMPSD-MP). The differential diagnosis is challenging due to overlap of their clinical and pathological features. Materials and methods: 116 OMPSD-MP patients were included in this cross-sectional study from November 2019 to February 2021, including oral lichen planus (OLP), oral lichenoid lesions (OLL), discoid lupus erythematosus (DLE), oral submucous fibrosis (OSF) and oral leukoplakia (OLK). The general information, clinical manifestation, histopathological and direct immunofluorescence (DIF) features were statistically analyzed and compared. Results: OLP was the major type of OMPSD-MP (64.7%), followed by OLL (25.0%), OLK (6.0%), DLE (2.6%) and OSF (1.7%), which were pooled as non-OLP group for further assessment. They shared many clinical and histological features in common. The rate of clinical-pathological diagnosis concordance was 73.5% for OLP, and 76.7% for total OMPSD-MP. DIF positive rate was significantly higher in OLP group than non-OLP group (76.0% vs. 41.5%, P < 0.001), in which the deposition of fibrinogen (Fib) and IgM were most frequently found. Conclusion: A significant overlap in clinical and histopathological features of OMPSD-MP was found, while DIF could be useful in differential diagnosis. Fib and IgM might be important immunopathological factors in OLP, which need further exploration.

Nutritional status in patients with active pulmonary tuberculosis and new nutritional risk screening model for active tuberculosis: a national, multicenter, cross-sectional study in China.

Background: Tuberculosis (TB) remains a significant challenge for public health and is closely associated with malnutrition; however, few studies have attempted to screen malnutrition among TB patients. The study aimed to evaluate the nutrition status and build a new nutritional screening model for active TB. Methods: A retrospective, multicenter, large cross-sectional study was conducted in China from 1 January 2020 to 31 December 2021. All included patients diagnosed with active pulmonary TB (PTB) were evaluated both by Nutrition Risk Screening 2002 (NRS 2002) and Global Leadership Initiative on Malnutrition (GLIM) criteria. Univariate and multivariate analyses were conducted to screen the risk factors associated with malnutrition, and a new screening risk model, mainly for TB patients, was constructed. Results: A total of 14,941 cases meeting the inclusion criteria were entered into the final analysis. The malnutrition risk rate among PTB patients in China was 55.86% and 42.70%, according to the NRS 2002 and GLIM, respectively. The inconsistency rate between the two methods was 24.77%. A total of 11 clinical factors, including elderly, low body mass index (BMI), decreased lymphocyte cells, taking immunosuppressive agents, co-pleural TB, diabetes mellitus (DM), human immunodeficiency virus (HIV), severe pneumonia, decreased food intake within a week, weight loss and dialysis were identified as independent risk factors of malnutrition based on multivariate analyses. A new nutritional risk screening model was constructed for TB patients with a diagnostic sensitivity of 97.6% and specificity of 93.1%. Conclusions: Active TB patients have severe malnutrition status according to screening by the NRS 2002 and GLIM criteria. The new screening model is recommended for PTB patients as it is more closely tailored to the characteristics of TB.