RESUMO
The early growth response 2 gene (EGR2) is part of a multigene family encoding Cys2His2 type zinc-finger proteins and may play a role in the regulation of cellular proliferation. Egr2, (also known as Krox20) is the mouse orthologue of human EGR2 and was first identified as an immediate-early response gene, encoding a protein that binds DNA in a sequence-specific manner and acts as a transcription factor. Stable expression of Egr2 is specifically associated with the onset of myelination in the peripheral nervous system (PNS). Egr2(-/-) mice display disrupted hindbrain segmentation and development, and a block of Schwann-cell differentiation at an early stage. We hypothesized that Egr2 may be a transcription factor affecting late myelin genes and that human myelinopathies of the PNS may result from mutations in EGR2. In support of this hypothesis, we have identified one recessive and two dominant missense mutations in EGR2 (within regions encoding conserved functional domains) in patients with congenital hypomyelinating neuropathy (CHN) and a family with Charcot-Marie-Tooth type 1 (CMT1).
Assuntos
Proteínas de Ligação a DNA/genética , Doenças Desmielinizantes/genética , Genes/genética , Fatores de Transcrição/genética , Adolescente , Adulto , Sequência de Aminoácidos , Doença de Charcot-Marie-Tooth/genética , DNA/análise , DNA/genética , DNA/isolamento & purificação , Análise Mutacional de DNA , Proteína 2 de Resposta de Crescimento Precoce , Saúde da Família , Feminino , Humanos , Proteínas Imediatamente Precoces/genética , Masculino , Linhagem , Mutação Puntual/genética , Mutação Puntual/fisiologia , Homologia de Sequência de Aminoácidos , Dedos de Zinco/genéticaRESUMO
OBJECTIVE: To determine the number of primary laminin alpha2 gene mutations and to conduct genotype/phenotype correlation in a cohort of laminin alpha2-deficient congenital muscular dystrophy patients. BACKGROUND: Congenital muscular dystrophies (CMD) are a heterogeneous group of muscle disorders characterized by early onset muscular dystrophy and a variable involvement of the CNS. Laminin alpha2 deficiency has been reported in about 40 to 50% of cases of the occidental, classic type of CMD. Laminin alpha2 is a muscle specific isoform of laminin localized to the basal lamina of muscle fibers, where it is thought to interact with myofiber membrane receptor, such as integrins, and possibly dystrophin-associated glycoproteins. METHODS: Seventy-five CMD patients were tested for laminin alpha2 expression by immunofluorescence and immunoblot. The entire 10 kb laminin alpha2 coding sequence of 22 completely laminin alpha2-deficient patients was screened for causative mutations by reverse transcription (RT)-PCR/single strand conformational polymorphisms (SSCP) analysis and protein truncation test (PTT) analysis followed by automatic sequencing of patient cDNA. Clinical data from the laminin alpha2-deficient patients were collected. RESULTS: Thirty laminin alpha2-negative patients were identified (40% of CMD patients tested) and 22 of them were screened for laminin alpha2 mutations. Clinical features of laminin alpha2-deficient patients were similar, with severe floppiness at birth, delay in achievement of motor milestones, and MRI findings of white matter changes with normal intelligence. Loss-of-function mutations were identified in 95% (21/22) of the patients studied. SSCP analysis detected laminin alpha2 gene mutations in about 50% of the mutant chromosomes; PTT successfully identified 75% of the mutations. A two base pair deletion mutation at position 2,096-2,097 bp was present in 23% of the patients analyzed. CONCLUSIONS: Our data suggest that the large majority of laminin alpha2-deficient patients show laminin alpha2 gene mutations.
Assuntos
Laminina/genética , Distrofias Musculares/congênito , Distrofias Musculares/genética , Sequência de Bases , Biópsia , Criança , Pré-Escolar , Análise Mutacional de DNA , Feminino , Imunofluorescência , Deleção de Genes , Genótipo , Humanos , Lactente , Laminina/análise , Masculino , Dados de Sequência Molecular , Músculo Esquelético/química , Músculo Esquelético/patologia , Distrofias Musculares/patologia , Mutação , Fenótipo , Polimorfismo GenéticoRESUMO
PURPOSE: To show the role of ultrasound (US) in distinguishing retropharyngeal abscess from adenitis in children. METHODS: Eleven infants and children had clinical symptoms suggestive of retropharyngeal infection. Radiographic evaluation included, lateral neck radiography (11/11), contrast-enhanced neck CT (10/11), contrast-enhanced neck MR (1/11), and real time US (11/11) patients. US was used to characterize masses as solid (adenitis) or complex (abscess) and for guiding intraoperative aspiration and drainage. RESULTS: Contrast CT and MR showed findings suspicious for abscess in all 11 cases. Only three children had surgically drainable abscesses. CT numbers within inflammatory masses did not distinguish adenitis from abscess. US was able to correctly diagnose abscess or adenitis in each case. CONCLUSION: Lateral neck radiography and contrast CT identify and localize retropharyngeal inflammatory masses in children. US, but not CT, distinguishes between adenitis and abscess and aids in intraoperative aspiration and drainage.
Assuntos
Abscesso/diagnóstico , Doenças Faríngeas/diagnóstico , Abscesso/diagnóstico por imagem , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Imageamento por Ressonância Magnética , Masculino , Doenças Faríngeas/diagnóstico por imagem , Tomografia Computadorizada por Raios X , UltrassonografiaRESUMO
Amoxapine is a second-generation tricyclic antidepressant structurally related to the neuroleptic loxapine. It was previously marketed as an alternative to traditional tricyclic antidepressants because of alleged shorter onset of action and fewer cardiotoxic effects. However, various adverse reactions, including cardiac dysrhythmias, renal failure, coma, seizures, and neuroleptic malignant syndrome, were reported during therapy or after acute overdose. A 14-year-old boy ingested 1900 mg of amoxapine and developed seizures, hypertension, hyperpyrexia, altered mental status, myoglobinuria, renal failure, and transient magnetic resonance imaging (MRI) changes suggestive of hypertensive encephalopathy and neuroleptic malignant syndrome. Since mitochondrial disorders can cause multisystem failure, including encephalopathy, renal tubular dysfunction, and myopathy, a transient, toxic disorder of mitochondrial function was considered as the basis for the patient's clinical and MRI changes.
Assuntos
Amoxapina/intoxicação , Antidepressivos Tricíclicos/intoxicação , Depressores do Sistema Nervoso Central/intoxicação , Etanol/efeitos adversos , Encefalomiopatias Mitocondriais/induzido quimicamente , Adolescente , Overdose de Drogas , Humanos , Hipertensão/induzido quimicamente , Imageamento por Ressonância Magnética , Masculino , Encefalomiopatias Mitocondriais/patologia , Mioglobinúria/induzido quimicamente , Insuficiência Renal/induzido quimicamenteRESUMO
In this report, the newest of the functional imaging methods, magnetoencephalography, is described, and its use in addressing the issue of brain reorganization for basic sensory and linguistic functions is documented in a series of 10 children and young adults. These patients presented with a wide variety of conditions, ranging from tumors and focal epilepsy to reading disability. In all cases, clear evidence of reorganization of the brain mechanisms of either somatosensory or linguistic functions or both was obtained, demonstrating the utility of magnetoencephalography in studying, completely noninvasively, the issue of plasticity in the developing brain.
Assuntos
Encéfalo/crescimento & desenvolvimento , Linguística , Magnetoencefalografia , Plasticidade Neuronal/fisiologia , Adolescente , Adulto , Percepção Auditiva , Neoplasias Encefálicas/patologia , Criança , Dislexia/patologia , Epilepsia/patologia , Potenciais Somatossensoriais Evocados , Feminino , Humanos , Masculino , Percepção VisualRESUMO
The diagnosis and treatment of epilepsy relies heavily on descriptions of behavioral changes noted during seizure episodes. A pilot study was completed to determine the frequency of occurrence of behaviors commonly associated with seizures in a pediatric population (n = 153). Caretakers of the children (ages = 4 months to 19 years) were asked to respond to a checklist containing 40 behavioral descriptors. Thirteen descriptors were found to differentiate between seizure and nonseizure events. Twelve of these behaviors were endorsed significantly more frequently by caretakers of children with seizures including the following: does not remember what happened, moves mouth funny, drools, jerking/twitching, becomes stiff, changes in breathing, stares off, bites or chews tongue, eyes look glassy, will not respond, mumbles or slurs words, and eyes or head turn to one side. One behavior, fidgets in seat, was significantly more associated with nonseizure episodes. The behavioral descriptors may be presented in a checklist format or incorporated within a clinical interview in primary care settings for initial screening of children with possible seizure disorders.
Assuntos
Convulsões/diagnóstico , Convulsões/psicologia , Adolescente , Adulto , Cuidadores/estatística & dados numéricos , Distribuição de Qui-Quadrado , Criança , Pré-Escolar , Diagnóstico Diferencial , Epilepsias Parciais/diagnóstico , Epilepsias Parciais/psicologia , Epilepsia Tipo Ausência/diagnóstico , Epilepsia Tipo Ausência/psicologia , Epilepsia Generalizada/diagnóstico , Epilepsia Generalizada/psicologia , Feminino , Humanos , Lactente , Masculino , Projetos Piloto , Papel do Doente , Comportamento EstereotipadoAssuntos
Eosinofilia/tratamento farmacológico , Polimiosite/tratamento farmacológico , Prednisona/administração & dosagem , Biópsia , Criança , Creatina Quinase/sangue , Relação Dose-Resposta a Droga , Esquema de Medicação , Eosinofilia/patologia , Eosinófilos/patologia , Feminino , Humanos , Músculos/patologia , Polimiosite/patologia , Prednisona/efeitos adversosRESUMO
A 53-year-old man with chronic myelogenous leukemia developed progressive proximal muscle weakness with electromyographic and histologic features consistent with polymyositis. Although the association of polymyositis with solid tumors is well recognized, an association with hematologic malignancies has not been firmly established. A survey of the world medical literature reveals one previously reported case of polymyositis and one of dermatomyositis associated with chronic myelogenous leukemia. We conclude that polymyositis does occur in association with chronic myelogenous leukemia. Recognition of this association is important, since treatment of polymyositis can be successful.
Assuntos
Leucemia Mielogênica Crônica BCR-ABL Positiva/complicações , Polimiosite/complicações , Eletromiografia , Humanos , Masculino , Pessoa de Meia-Idade , Músculos/patologia , Músculos/fisiopatologia , Polimiosite/diagnósticoRESUMO
PURPOSE: To determine possible cognitive and behavioral effects of antiepileptic drug (AED) therapy by assessing children with newly diagnosed epilepsy before and after initiation of treatment. A comparison group of children with diabetes mellitus (DM) was included to control for the effects of practice, maturation, and chronic illness. METHODS: Baseline neuropsychological assessments were completed for children with epilepsy (n = 37) and children with DM (n = 26) recruited through outpatient clinics at a regional children's hospital. Children were reevaluated 6 months from baseline testing. At follow-up, children with epilepsy had therapeutic AED levels and controlled seizures. Statistical analysis included a between-group repeated measures ANCOVA with pretest scores serving as the covariate. RESULTS: Significant differences between groups were not found for any cognitive or behavioral factors, including attention (p < 0.24), immediate memory (p < 0.24), delayed memory (p < 0.10), complex motor speed (p < 0.19), or behavior problems (p < 0.89). CONCLUSIONS: Changes in performance on cognitive and behavioral measures were not different for children treated with AEDs and controls. Although adverse effects may be associated with prolonged treatment, results would not suggest adverse effects from AED monotherapy during the first 6 months of therapy.
Assuntos
Anticonvulsivantes/efeitos adversos , Comportamento Infantil/efeitos dos fármacos , Cognição/efeitos dos fármacos , Epilepsia/tratamento farmacológico , Adolescente , Fatores Etários , Anticonvulsivantes/uso terapêutico , Atenção/efeitos dos fármacos , Criança , Transtornos do Comportamento Infantil/induzido quimicamente , Transtornos do Comportamento Infantil/diagnóstico , Transtornos Cognitivos/induzido quimicamente , Transtornos Cognitivos/diagnóstico , Diabetes Mellitus/diagnóstico , Epilepsia/diagnóstico , Feminino , Seguimentos , Humanos , Masculino , Memória/efeitos dos fármacos , Testes NeuropsicológicosRESUMO
Ten laminin alpha2-deficient patients were identified by both immunofluorescence and immunoblotting (30% of congenital muscular dystrophy patients tested). Three of the laminin alpha2-deficient patients were carrying a diagnosis of infantile polymyositis prior to immunostaining studies. The clinical features in the 10 merosin-deficient patients were homogeneous, with severe floppiness at birth, delay in achievement of motor milestones, and magnetic resonance imaging findings of white matter changes with normal intelligence. The 10-kb laminin alpha2-coding sequence was screened for causative mutations by reverse transcriptase-polymerase chain reaction/single-stranded conformational polymorphism analysis in muscle biopsy specimens from 5 patients, followed by automatic sequencing of aberrant conformers. Clear loss-of-function deletion mutations were identified in both alleles of 1 patient. Muscle histopathology in this patient showed a striking inflammatory infiltrate of T cells and B cells. Reexamination of biopsy specimens from other laminin alpha2-deficient patients showed minor signs of inflammation in each. Based on these findings and the histological and clinical picture suggesting failure of muscle regeneration, a pathogenesis model for this major subset of congenital muscular dystrophy is proposed. Our data show that muscle histopathology showing a neonatal inflammatory process should be considered consistent with congenital muscular dystrophy.
Assuntos
Laminina/deficiência , Músculo Esquelético/patologia , Distrofias Musculares/genética , Distrofias Musculares/fisiopatologia , Polimorfismo Conformacional de Fita Simples , Polimiosite/fisiopatologia , Sequência de Bases , Biópsia , Criança , Pré-Escolar , Desoxirribonucleases de Sítio Específico do Tipo II , Eletromiografia , Feminino , Humanos , Lactente , Recém-Nascido , Inflamação , Laminina/genética , Pessoa de Meia-Idade , Atividade Motora , Músculo Esquelético/fisiopatologia , Distrofias Musculares/congênito , Núcleo Familiar , Linhagem , Mutação Puntual , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição , Deleção de SequênciaRESUMO
The myogenin gene encodes an evolutionarily conserved basic helix-loop-helix transcription (bHLH) factor that is required for differentiation of skeletal muscle, and its homozygous deletion in mice results in perinatal death from respiratory failure due to the lack of muscle fibers. Since the histology of skeletal muscle in myogenin null mice is reminiscent of that found in severe congenital myopathy patients, many of whom also die of respiratory complications, we sought to test the hypothesis that an aberrant human myogenin (myf4) coding region could be associated with some congenital myopathy conditions. With PCR amplification, we found similarly sized PCR products for the three exons of the myogenin gene in DNA from 37 patient and 40 control individuals. In contrast to previously reported sequencing of human myogenin (myf4), we describe with automated sequencing several base differences in flanking and coding regions plus an additional 659 and 498 bp in the first and second introns, respectively, in all 37 patient and 40 control samples. We also find a variable length (CA)-dinucleotide repeat in the second intron, which may have utility as a marker for future linkage studies. In summary, no causative mutations were detected in the myogenin coding locus of genomic DNA from 37 patients with severe congenital myopathy.
Assuntos
Sequências Hélice-Alça-Hélice , Miogenina/genética , Doenças Neuromusculares/genética , Fatores de Transcrição/genética , Sequência de Bases , DNA Complementar , Humanos , Lactente , Dados de Sequência Molecular , Mutagênese , Doenças Neuromusculares/congênitoRESUMO
Neurofilament light chain polypeptide (NEFL) is one of the most abundant cytoskeletal components of the neuron. Mutations in the NEFL gene were recently reported as a cause for autosomal dominant Charcot-Marie-Tooth type 2E (CMT2E) linked to chromosome 8p21. In order to investigate the frequency and phenotypic consequences of NEFL mutations, we screened 323 patients with CMT or related peripheral neuropathies. We detected six disease associated missense mutations and one 3-bp in-frame deletion clustered in functionally defined domains of the NEFL protein. Patients have an early onset and often a severe clinical phenotype. Electrophysiological examination shows moderately to severely slowed nerve conduction velocities. We report the first nerve biopsy of a CMT patient with a de novo missense mutation in NEFL, and found an axonal pathology with axonal regeneration clusters and onion bulb formations. Our findings provide further evidence that the clinical variation observed in CMT depends on the gene mutated and the specific type of mutation, and we also suggest that NEFL mutations need to be considered in the molecular evaluation of patients with sporadic or dominantly inherited CMT.