RESUMEN
PURPOSE:: To evaluate the contribution of L-arginine oral or topical rout of administration in the surgical wound healing process. METHODS:: L-arginine was orally or topically administrated to mice after a laparotomy model procedure. The wounds were analyzed to evaluate the granulation tissue by HE analysis, collagen deposition, iNOS and cytokines production by immunochemisyry on wound progress. Mice used in this model were healthy, immunosupressed or diabetic and all of them were treated with different concentration of L-arginine and rout of administration. RESULTS:: Suggested that groups treated with L-arginine orally or topically improved wound repair when compared with non-treatad mice. L- arginine treatment stimulated TGF-ß and restricted NO production leading to a mild Th1 response and collagen deposition in injured area, when it was orally administrated. Topical administration decreased IL-8 and CCR1 expression by wound cells but did not interfere with TNF-α and IL-10 production, ratifying the decrease of inflammatory response, the oral administration however, presented a higher iNOS and TGF-ß expression then. L-arginine treatment also improved the improved the wound healing in immunosupressed or diabetic mice. CONCLUSION:: L-arginine administrated orally or topically can be considered an important factor in the recuperation of tissues.
Asunto(s)
Arginina/administración & dosificación , Citocinas/metabolismo , Óxido Nítrico Sintasa de Tipo II/biosíntesis , Herida Quirúrgica/tratamiento farmacológico , Factor de Crecimiento Transformador beta/biosíntesis , Cicatrización de Heridas/efectos de los fármacos , Administración Oral , Administración Tópica , Animales , Arginina/metabolismo , Colágeno/biosíntesis , Diabetes Mellitus Experimental/metabolismo , Modelos Animales de Enfermedad , Huésped Inmunocomprometido , Inflamación/metabolismo , Masculino , Ratones , Óxido Nítrico/biosíntesis , Heridas y Lesiones/patologíaRESUMEN
ABSTRACT PURPOSE: To evaluate the contribution of L-arginine oral or topical rout of administration in the surgical wound healing process. METHODS: L-arginine was orally or topically administrated to mice after a laparotomy model procedure. The wounds were analyzed to evaluate the granulation tissue by HE analysis, collagen deposition, iNOS and cytokines production by immunochemisyry on wound progress. Mice used in this model were healthy, immunosupressed or diabetic and all of them were treated with different concentration of L-arginine and rout of administration. RESULTS: Suggested that groups treated with L-arginine orally or topically improved wound repair when compared with non-treatad mice. L- arginine treatment stimulated TGF-β and restricted NO production leading to a mild Th1 response and collagen deposition in injured area, when it was orally administrated. Topical administration decreased IL-8 and CCR1 expression by wound cells but did not interfere with TNF-α and IL-10 production, ratifying the decrease of inflammatory response, the oral administration however, presented a higher iNOS and TGF-β expression then. L-arginine treatment also improved the improved the wound healing in immunosupressed or diabetic mice. CONCLUSION: L-arginine administrated orally or topically can be considered an important factor in the recuperation of tissues.