RESUMEN
BACKGROUND: MicroRNAs (miRNAs) and other epigenetic modifications play fundamental roles in all eukaryotic biological processes. DNA damage repair is a key process for maintaining the genomic integrity of different organisms exposed to diverse stresses. However, the reaction of miRNAs in the DNA damage repair process is unclear. RESULTS: In this study, we found that the simultaneous mutation of zinc finger DNA 3'-phosphoesterase (ZDP) and AP endonuclease 2 (APE2), two genes that play overlapping roles in active DNA demethylation and base excision repair (BER), led to genome-wide alteration of miRNAs. The transcripts of newly transcribed miRNA-encoding genes (MIRs) decreased significantly in zdp/ape2, indicating that the mutation of ZDP and APE2 affected the accumulation of miRNAs at the transcriptional level. In addition, the introduction of base damage with the DNA-alkylating reagent methyl methanesulfonate (MMS) accelerated the reduction of miRNAs in zdp/ape2. Further mutation of FORMAMIDOPYRIMIDINE DNA GLYCOSYLASE (FPG), a bifunctional DNA glycosylase/lyase, rescued the accumulation of miRNAs in zdp/ape2, suggesting that the accumulation of DNA damage repair intermediates induced the transcriptional repression of miRNAs. CONCLUSIONS: Our investigation indicates that the accumulation of DNA damage repair intermediates inhibit miRNAs accumulation by inhibiting MIR transcriptions.
Asunto(s)
Proteínas de Arabidopsis , Arabidopsis , MicroARNs , Arabidopsis/genética , Daño del ADN , Reparación del ADN/genética , Transcripción Genética , MicroARNs/genética , Endonucleasas/genética , Proteínas de Arabidopsis/genéticaRESUMEN
El presente artículo tiene la intención de exponer algunos de los resultados obtenidos durante la realización de una investigación psicopedagógica dirigida a estudiar los efectos que produce la estimulación del razonamiento analógico, en los límites de la Zona de Desarrollo Próximo de los escolares, durante el aprendizaje de una materia escolar a través de un método denominado Aprendizaje por Transferencia Analógica, sobre el desarrollo de los procesos implicados en este tipo de razonamiento y su repercusión en la calidad de los aprendizajes de los estudiantes.
The present article has the intention of exposing some of the results obtained during the realization of an psicopedagogical investigation directed to study the effects that produces the stimulation of the analogical reasoning, in the limits of the scholars' Proximal Developmental Zone and during the learning of a school matter through a method called Learning by Analogical Transfer, on the development of the processes implied in this reasoning kind and its consequences in the quality of the students' learning.