Smartphone-assisted hydrogel platform based on BSA-CeO2 nanoclusters for dual-mode determination of acetylcholinesterase and organophosphorus pesticides.

A dual-mode sensor was developed for detecting acetylcholinesterase (AChE) and organophosphorus pesticides (OPs) via bifunctional BSA-CeO2 nanoclusters (NCs) with oxidase-mimetic activity and fluorescence property. The dual-mode sensor has the characteristics of self-calibration and self-verification, meeting the needs of different detection conditions and provide more accurate results. The colorimetric sensor and fluorescence sensor have been successfully used for detecting AChE with limit of detection (LOD) of 0.081 mU/mL and 0.056 mU/mL, respectively, while the LOD for OPs were 0.9 ng/mL and 0.78 ng/mL, respectively. The recovery of AChE was 93.9-107.2% and of OPs was 95.8-105.0% in actual samples. A novel strategy was developed to monitor pesticide residues and detect AChE level, which will motivate future work to explore the potential applications of multifunctional nanozymes.

Development and Validation of Nutrition Environment Scoring for Chinese Style University/Work-Site Canteens (NESC-CC) and Oil-Salt Visual Analogue Scale (OS-VAS).

The assessment of the use of cooking oil/fat and salt in dining food is an unsolved issue in non-quantitative nutrition environment evaluation, and the nutrition environment of Chinese-style dining establishments has not been effectively assessed. This study developed two evaluation tools: the Nutrition Environment Scoring for Chinese Style University/Work-site Canteens (NESC-CC) and the Oil-Salt Visual Analogue Scale (OS-VAS) and applied them in nine university canteens. The NESC-CC, which includes comprehensive items from the healthiness of food availability, cooking methods to other factors that support healthy choices, is featured by adjusting the scoring categories and items to suit Chinese food culture and canteen operation model. The OS-VAS is a novel virtual analog scale (VAS) based on the fuzzy judgement of the diners. It requires the randomly recruited respondents to rate their personal preference for salty taste/greasy food preference, overall saltiness/greasiness of canteen dishes, and personal demands for salt/cooking oil reduction. The oil use score, the salt use score, and the total score are derived from the given formula. The field tests of the NESC-CC in nine university canteens at three time points showed that this tool was able to effectively distinguish the nutrition environment of the Chinese food-style canteens with good reliability and validity. The result of OS-VAS scoring achieved a good resolution of the overall salt and oil/fat use and confirmed our hypothesis on the fuzzy judgement of the diners These tools are suitable for the comprehensive evaluation of Chinese-style canteens and have the potential to be applied to more group-meal-providing establishments.

Bioconjugates of versatile ß-diketonate-lanthanide complexes as probes for time-gated luminescence and magnetic resonance imaging of cancer cells in vitro and in vivo.

Magnetic resonance imaging (MRI) and optical imaging (OI) are attractive for constructing bimodal probes due to their complementary imaging characteristics. The combination of these two techniques could be a useful tool to simultaneously obtain both anatomical and molecular information as well as to significantly improve the accuracy of detection. In this study, we found that ß-diketonate-lanthanide complexes, BHHBCB-Ln3+, could covalently bind to proteins to exhibit long-lived and intense luminescence (Ln3+ = Eu3+, τ = 0.52 ms, Φ = 0.40) and remarkably high relaxivity (Ln3+ = Gd3+, r1 = 35.67 mM-1 s-1, r2 = 43.25 mM-1 s-1) with excellent water solubility, stability and biocompatibility. Hence, we conjugated BHHBCB-Ln3+ with a tumor-targetable biomacromolecule, transferrin (Tf), to construct the probes, Tf-BHHBCB-Ln3+, for time-gated luminescence (TGL, Ln3+ = Eu3+) and MR (Ln3+ = Gd3+) imaging of cancerous cells in vitro and in vivo. As expected, the as-prepared probes showed high specificity to bind with the transferrin receptor-overexpressed cancerous cells, to enable the probe molecules to be accumulated in these cells. Using Tf-BHHBCB-Ln3+ as probes, the cultured cancerous cells and the tumors in tumor-bearing mice have been clearly visualized by background-free TGL and in vivo MR imaging. The research outcomes suggested the potential of ß-diketonate-lanthanide complexes for use in constructing bimodal TGL/MR imaging bioprobes.

Lateral injection using a Whitacre needle with patients in the lateral decubitus position maintained for a prolonged time period produces symmetric sensory block.

BACKGROUND: The directional flow of injection through a Whitacre needle can be used to modify the level of sensory blockade. We hypothesized that injection of hyperbaric local anesthetic through a Whitacre needle with the bevel oriented laterally can produce a more symmetric sensory block. METHODS: Patients scheduled for lower limb surgery under spinal anesthesia with the patient in lateral decubitus position were randomized to receive 10 mg, 0.5% hyperbaric bupivacaine with the Whitacre needle orifice in 1 of 2 orientations, cephalad and lateral. The patient's position was maintained for 15 min after the injection, and sensory blocks were recorded. The primary outcome was the sensory levels between the dependent and nondependent side. The Wilcoxon-Mann-Whitney U-test odds was used to compare unpaired nonparametric data. For the paired samples, 95% confidence intervals (CI) of differences between group medians were calculated using the Hodges-Lehman estimator for the median difference in number of blocked segments. RESULTS: There was no significant difference in block level between dependent and nondependent sides in the lateral group. The Hodges-Lehmann point estimator was 0.5% and 95% CI was 0-2.5, suggesting a more symmetric sensory block in patients in the lateral decubitus position maintained for a sufficient period of time. A significantly lower level of blockade was noticed on the nondependent side compared to the dependent side in the cephalad group. The Hodges-Lehmann point estimator was 2.5 segments and 95% CI was 0.5-5. CONCLUSIONS: Injection of 10 mg of 0.5% hyperbaric bupivacaine with the bevel of the Whitacre needle oriented laterally produces more symmetric sensory levels of blockade between the dependent and nondependent sides.