RESUMO
In this work, we have implemented a polymerase and endonuclease synergetic amplification reaction in living cells for intracellular miRNA imaging by using biomineralized ZIF-8 NPs.
Assuntos
Biomineralização , DNA Polimerase Dirigida por DNA/metabolismo , Imidazóis/química , MicroRNAs/metabolismo , Nanopartículas/química , Zeolitas/química , Linhagem Celular Tumoral , Sobrevivência Celular , Endonucleases/química , Endonucleases/metabolismo , Humanos , Imagem MolecularRESUMO
In this work, we have developed a novel DNAzyme motor initiated by endogenous enzyme for sensitive imaging of intracellular RNase H activity.
Assuntos
DNA Catalítico/metabolismo , Imagem Óptica , Ribonuclease H/metabolismo , Sobrevivência Celular , Células HeLa , Células Hep G2 , Humanos , Células MCF-7 , Microscopia Confocal , Microscopia de FluorescênciaRESUMO
Glutathione (GSH) plays an important role in cells, which is an essential endogenous antioxidant. Here, we have developed a new detection platform to analyze GSH levels. In our system, fluorescent polydopamine (PDA) nanoparticles, as signal indicators, were obtained by oxidation through cobalt oxyhydroxide (CoOOH) nanosheets. When CoOOH was present, CoOOH could quickly oxidize dopamine to fluorescent PDA nanoparticles. However, once GSH existed, CoOOH nanosheets were decomposed into Co2+, and oxidation between CoOOH and dopamine was prevented with weaker fluorescence occurring. Thus, we could realize detection of the GSH concentration according to the decreased fluorescence value of the fluorescent polydopamine. This method provides a fast, simple, high sensitivity and desirable selectivity platform for GSH monitoring.