RESUMO
Garcinia cambogia is one of the most commonly used anti-obesity dietary supplements, and hydroxycitric acid (HCA) is a major constituent in the commercial preparations of Garcinia. High doses of HCA are often consumed without much awareness of its pharmacokinetic and toxicokinetic parameters, and therefore, a complete understanding of its effects is lacking. The first step in understanding these parameters is the availability of a reliable bioanalytical method. Here, we present the first report on a UPLC-MS/MS method for analysis of HCA in rat plasma after a simplified and cost-effective protein precipitation. Chromatographic separation of the analytes in the supernatant was achieved using hydrophilic interaction liquid chromatography, where mass parameters were optimized and a rapid 5-min quantitative assay was developed. The method was highly sensitive, accurate, precise and linear in the concentration range of 10.5-5000 ng/mL (validated according to the United States Food and Drug Administration guidelines). Further, the method was successfully used to describe the pharmacokinetic profile of HCA in rat plasma after the administration of pure HCA and commercial Garcinia preparations.
Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Citratos/sangue , Citratos/farmacocinética , Garcinia , Espectrometria de Massas em Tandem/métodos , Administração Oral , Animais , Citratos/química , Suplementos Nutricionais , Modelos Lineares , Masculino , Extratos Vegetais/administração & dosagem , Extratos Vegetais/química , Extratos Vegetais/farmacocinética , Ratos , Ratos Wistar , Reprodutibilidade dos Testes , Sensibilidade e EspecificidadeRESUMO
AIM: The main goal in Crohn´s disease (CD) is a sustained suppression of inflammatory activity associated with mucosa healing in endoscopic evaluation. During clinical routine, there are small numbers of good markers to monitor inflammatory activity under treatment. We postulated that Oral 67Gallium Citrate Scintigraphy is able to mark inflammatory disease in mucosa and deep inflammation in CD, when used in oral form. OBJECTIVE: Measure the accuracy of Oral 67Gallium Citrate Scintigraphy in intestinal inflammatory activity of Crohn´s disease. PATIENTS AND METHODS: In a prospective consecutive cross-sectional study from January 2018 to June 2019, the ileocolonic region of 32 patients with CD were studied by dividing into four regions of interest (ROI) from the ileum to the rectum. A total of 128 intestinal segments were analyzed in cluster data. Accuracy values of Oral 67Gallium Scintigraphy and colonoscopy tests were evaluated with the histological reference test. Values of the respective receiver operating characteristic (ROC) curves were obtained and compared. The reliability between the tests was evaluated by Kappa statistical with the segment-level analyses using variance adjustments. All statistical analyses were performed with a test significance level of 0.05. RESULTS: The study population included 32 patients with CD (10 men, 22 women; average age 39 years). Disease time was five years on average. Anti-TNFs treatment was found in 71%. The most found phenotype of the Montreal classification was L3. Differences in ROC curves for colonoscopy (0.94) and Oral 67Ga Scintigraphy (0.96) did not show significant value (p = 0.32). The sensitivity of scintigraphy to detect intestinal inflammatory activity in CD was 64%, specificity of 96% and accuracy of 84%. A high agreement was found between oral scintigraphy and histological measurements with kappa = 0.64. CONCLUSIONS: Oral 67Ga Scintigraphy had similar accuracy and agreement compared to colonoscopy in the identification of inflammatory activity in Crohn´s Disease. This new approach may be useful and less invasive for long term follow-ups.
Assuntos
Citratos/administração & dosagem , Doença de Crohn/complicações , Radioisótopos de Gálio/administração & dosagem , Gálio/administração & dosagem , Inflamação/diagnóstico por imagem , Cintilografia/métodos , Administração Oral , Adulto , Antineoplásicos/farmacologia , Citratos/química , Estudos Transversais , Feminino , Gálio/química , Radioisótopos de Gálio/química , Humanos , Íleo/metabolismo , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Curva ROC , Compostos Radiofarmacêuticos/administração & dosagem , Compostos Radiofarmacêuticos/química , Reto/metabolismo , Reprodutibilidade dos Testes , Medição de Risco , Resultado do Tratamento , Fator de Necrose Tumoral alfa/metabolismoRESUMO
Some nanoparticles (NPs) have been shown to disrupt intestinal microvilli morphology in vitro, an alteration that could potentially affect nutrient absorption and barrier properties. This study aimed at evaluating the potential effect of CeO2 NPs (4-8â¯nm, citrate stabilized) on Caco-2 microvilli morphology. In addition to the standard Caco-2 cell clone, the C2BBe1 clone was used, as it is considered to develop a more homogeneous cellular morphology. Semiautomated microvilli density quantification and a new cell scoring approach were used to evaluate scanning electron microscopy (SEM) images. The quantification method made use of the whole micrograph surface, avoiding the need to choose subareas for analysis, and increasing the representativeness of the results when compared to previous studies. The main advantage of the scoring system is that it informs on the intercellular variability within a cell preparation. Benzalkonium was used as a positive control inducing toxicity and morphological alterations on microvilli. After three-week differentiation, Caco-2 cells were exposed to 100⯵g/mL of CeO2 NPs for 24â¯h. The integrity of the membrane was evaluated by transepithelial electrical resistance (TEER) and thereafter processed for its observation by SEM. Results showed that both the standard Caco-2 clone and the C2BBe1 clone present notable morphological heterogeneity. The two evaluation approaches were able to identify morphological effects caused by the positive control, but did not detect statistically significant morphological alterations after exposure to CeO2 NPs.
Assuntos
Cério/toxicidade , Mucosa Intestinal/efeitos dos fármacos , Microvilosidades/efeitos dos fármacos , Nanopartículas/toxicidade , Células CACO-2 , Cério/química , Citratos/química , Citratos/toxicidade , Humanos , Nanopartículas/químicaRESUMO
The present work focuses on application of an investigational approach to assess the hot-processability of pharmaceutical-grade polymers with a potential for use in the manufacturing of reservoir drug delivery systems via micromolding, and the performance of resulting molded barriers. An inert thermoplastic polymer, ethylcellulose (EC), widely exploited for preparation of prolonged-release systems, was employed as a model component of the release-controlling barriers. Moldability studies were performed with plasticized EC, as such or in admixture with release modifiers, by the use of disk-shaped specimens ≥ 200⯵m in thickness. The disks turned out to be a suitable tool for evaluation of the dimensional stability and diffusional barrier performance of the investigated materials after demolding. The effect of the amount of triethyl citrate, used as a plasticizer, on hot-processability of EC was assessed. The rate of a model drug diffusion across the polymeric barriers was shown to be influenced by the extent of porosity from the incorporated additives. The investigational approach proposed, of simple and rapid execution, holds potential for streamlining the development of prolonged-release systems produced by micromolding in the form of drug reservoirs, with no need for molds and molding processes to be set up on a case-by-case basis.
Assuntos
Celulose/análogos & derivados , Excipientes/química , Celulose/química , Citratos/química , Preparações de Ação Retardada/química , Composição de Medicamentos , Temperatura Alta , Plastificantes/química , Polivinil/química , ReologiaRESUMO
Diabetes mellitus comprises a group of metabolic abnormalities due to insulin deficiency and/or resistance. Obesity contributes to diabetes, with a strong causal relationship existing between diabetes and insulin resistance, especially in patients with Diabetes mellitus II. Adipocytes emerge as key constituents of adipose tissue physiology. In their pre-mature form to mature state transformation, adipocytes fully exemplify one of the key adipogenic actions of insulin. Poised to a) gain insight into adipogenesis leading to antidiabetic factors, and b) investigate adipogenesis through careful examination of insulin contributions to interwoven mechanistic pathways, a systematic comparative study was launched involving well-defined metal-citrates (zinc and vanadium), the chemical reactivity of which was in line with their chemistry under physiological conditions. Selection of the specific compounds was based on their common aqueous coordination chemistry involving the physiological chelator citric acid. Cellular maturation of pre-adipocytes to their mature form was pursued in the presence-absence of insulin and employment of closely linked genetic targets, key to adipocyte maturation (Peroxisome proliferator-activated receptor gamma (PPAR-γ), Glucose transporter 1,3,4 (GLUT 1,3,4), Adiponectin (ADIPOQ), Glucokinase (GCK), and Insulin receptor (INS-R)). The results show a) distinct adipogenic biological profiles for the metalloforms involved in a dose-, time- and nature-dependent manner, and b) metal ion-specific adipogenic response-signals at the same or higher level than insulin toward all selected targets. Collectively, the foundations have been established for future exploitation of the distinct metal-specific adipogenic factors contributing to the functional maturation of adipose tissue and their use toward hyperglycemic control in Diabetes mellitus.
Assuntos
Adipócitos/metabolismo , Adipogenia/efeitos dos fármacos , Citratos , Regulação da Expressão Gênica/efeitos dos fármacos , Hipoglicemiantes , Vanádio , Células 3T3-L1 , Adipócitos/patologia , Animais , Citratos/química , Citratos/farmacologia , Diabetes Mellitus/tratamento farmacológico , Diabetes Mellitus/metabolismo , Diabetes Mellitus/patologia , Humanos , Hipoglicemiantes/química , Hipoglicemiantes/farmacologia , Camundongos , Vanádio/química , Vanádio/farmacologiaRESUMO
Liver failure is a serious and often deadly disease often requiring MARS (Molecular Adsorbent Recirculating System) therapy. Choosing the safe and effective method of anticoagulation during artificial liver support systems seems to be very difficult and extremely important. The aim of this study was to assess effectiveness and safety of regional anticoagulation with citrate in liver failure patients during MARS. We used a single center observational study. We analyzed 158 MARS sessions performed in 65 patients: 105 (66.5%) sessions in 41 patients with heparin anticoagulation, 40 (25.3%) sessions in 19 patients with citrate, and 13 (8%) sessions in only five patients without anticoagulation, that were excluded from part of the analysis. To determine the effectiveness of regional anticoagulation with citrate, probability of filter survival and changes in laboratory parameters were analyzed according to the applied method of anticoagulation. The safety of citrate was determined by Ca/Ca2+ ratio, acid-base balance, bleeding complications, and the need for blood product transfusions. The probability of filter survival in the citrate group was 94% and in the heparin group 82% (P = 0.204). There was no relationship between the method of anticoagulation and effectiveness of MARS therapy in lowering the levels of the analyzed parameters. Only one patient had a Ca/Ca2+ ratio higher than he safety margin. There were no statistically significant changes in pH and lactate level irrespective of anticoagulation; bicarbonate dropped significantly only in the heparin group (P = 0.03). The frequency of bleeding complications and the need for transfusions did not differ significantly between groups. Regional anticoagulation with citrate can be an effective and safe method of anticoagulation during MARS therapy, but requires attentive monitoring and further studies in liver failure patients.
Assuntos
Anticoagulantes/uso terapêutico , Transtornos da Coagulação Sanguínea/prevenção & controle , Citratos/uso terapêutico , Soluções para Diálise/uso terapêutico , Hemofiltração/efeitos adversos , Falência Hepática/terapia , Equilíbrio Ácido-Base , Adulto , Idoso , Idoso de 80 Anos ou mais , Anticoagulantes/química , Bicarbonatos/sangue , Transtornos da Coagulação Sanguínea/etiologia , Citratos/química , Soluções para Diálise/química , Feminino , Hemofiltração/métodos , Heparina/química , Heparina/uso terapêutico , Humanos , Lactatos/sangue , Falência Hepática/sangue , Fígado Artificial/efeitos adversos , Masculino , Pessoa de Meia-Idade , Albumina Sérica/química , Adulto JovemRESUMO
Developing a pediatric oral formulation with an age-appropriate dosage form and taste masking of naturally bitter active pharmaceutical ingredients (APIs) are key challenges for formulation scientists. Several techniques are used for taste masking of bitter APIs to improve formulation palatability; however, not all the techniques are applicable to pediatric dosage forms because of the limitations on the kind and concentration of the excipients that can be used. Hot-melt extrusion (HME) technology is used successfully for taste masking of bitter APIs and overcomes some of the limitations of the existing taste-masking techniques. Likewise, analytical taste assessment is an important quality control parameter evaluated by several in vivo and in vitro methods, such as the human taste panel, electrophysiological methods, electronic sensor, and animal preference tests to aid in selecting a taste-masked formulation. However, the most appropriate in vivo method to assess the taste-masking efficacy of pediatric formulations remains unknown because it is not known to what extent the human taste panel/electronic tongue can predict the palatability in the pediatric patients. The purpose of this study was to develop taste-masked caffeine citrate extrudates via HME and to demonstrate the wide applicability of a single bottle-test rat model to record and compare the volume consumed of the taste-masked solutions to that of the pure API. Thus, this rat model can be considered as a low-cost alternative taste-assessment method to the most commonly used expensive human taste panel/electronic tongue method for pediatric formulations.
Assuntos
Cafeína/administração & dosagem , Cafeína/química , Citratos/administração & dosagem , Citratos/química , Paladar/fisiologia , Administração Oral , Animais , Química Farmacêutica/métodos , Nariz Eletrônico , Excipientes/química , Humanos , Masculino , Pediatria , Controle de Qualidade , Ratos , Ratos Sprague-Dawley , Soluções/química , Tecnologia Farmacêutica/métodosRESUMO
Starch suspensions were crosslinked with trisodium citrate for either 0 or 17h, gelled and then freeze-dried to corresponding aerogels. The aerogel from the 17h-crosslinked suspension was loaded with the antifungal compound, trans-2-hexenal, and coated with the surfactant, sorbitan monooleate. Aerogel hardness was increased by the citrate-mediated crosslinking, whereas its adhesiveness decreased. Starch gelation decreased the crystallinity index (CrI) from 59% to ≈23%; however, the pre-gelation crosslinking resulted in a higher CrI value (i.e. ≈38%) for the aerogel. The voids at the internal microstructure of the 17h-crosslinked aerogel were more uniform and coating with surfactant closed the surface openings. The latter accordingly resulted in a more sustained release of the volatile, trans-2-hexenal, from the crosslinked starch aerogel and led to slower lethality of Aspergillus parasiticus cells inoculated on pistachio nuts compared with the non-coated condition.
Assuntos
Antifúngicos/química , Géis/química , Amido/química , Compostos Orgânicos Voláteis/química , Aldeídos/química , Antifúngicos/farmacologia , Aspergillus/química , Aspergillus/efeitos dos fármacos , Citratos/química , Materiais Revestidos Biocompatíveis/química , Estudos de Viabilidade , Contaminação de Alimentos/prevenção & controle , Microbiologia de Alimentos , Liofilização , Hexoses/química , Viabilidade Microbiana/efeitos dos fármacos , Nozes/microbiologia , Pistacia/microbiologia , Amido/farmacologia , TensoativosRESUMO
Citrated Sprague-Dawley rat blood plasma was used as a biologically relevant exposure medium to assess the acellular toxic potential of two metal oxide engineered nanomaterials (ENMs), zinc oxide (nZnO), and cerium oxide (nCeO2). Plasma was incubated at 37 °C for up to 48 h with ENM concentrations ranging between 0 and 200 mg/L. The degree of ENM-induced oxidation was assessed by assaying for reactive oxygen species (ROS) levels using dichlorofluorescein (DCF), pH, ferric reducing ability of plasma (FRAP), lipase activity, malondialdehyde (MDA), and protein carbonyls (PC). Whereas previous in vitro studies showed linear-positive correlations between ENM concentration and oxidative damage, our results suggested that low concentrations were generally pro-oxidant and higher concentrations appeared antioxidant or protective, as indicated by DCF fluorescence trends. nZnO and nCeO2 also affected pH in a manner dependent on concentration and elemental composition; higher nZnO concentrations maintained a more alkaline pH, while nCeO2 tended to decrease pH. No other biomarkers of oxidative damage (FRAP, MDA, PC, lipase activity) showed changes at any ENM concentration or time-point tested. Differential dissolution of the two ENMs was also observed, where as much as â¼31.3% of nZnO was instantaneously dissolved to Zn2+ and only negligible nCeO2 was degraded. The results suggest that the direct oxidative potential of nZnO and nCeO2 in citrated rat blood plasma is low, and that a physiological or immune response is needed to generate appreciable damage biomarkers. The data also highlight the need for careful consideration when selecting a model for assessing ENM toxicity.
Assuntos
Cério/toxicidade , Nanoestruturas/toxicidade , Plasma/efeitos dos fármacos , Óxido de Zinco/toxicidade , Animais , Soluções Tampão , Cério/sangue , Citratos/química , Concentração de Íons de Hidrogênio , Técnicas In Vitro , Plasma/metabolismo , Ratos Sprague-Dawley , Espécies Reativas de Oxigênio/metabolismo , Citrato de Sódio , Óxido de Zinco/sangueRESUMO
We report an electric pressure cooker for large-scale synthesis of water-soluble Cu-In-S/ZnS core/shell quantum dots. Low-cost thioglycolic acid and sodium citrate were used as the dual stabilizers. â¼3 grams of quantum dots with a tunable emission from 545 to 610 nm and quantum yield up to 40% were obtained in a batch.
Assuntos
Cobre/química , Índio/química , Nanotecnologia/métodos , Pontos Quânticos , Sulfetos/química , Enxofre/química , Compostos de Zinco/química , Citratos/química , Desenho de Equipamento , Células Hep G2 , Humanos , Microscopia Confocal , Microscopia Eletrônica de Transmissão , Microscopia de Fluorescência , Nanopartículas , Semicondutores , Citrato de Sódio , Solubilidade , Espectrofotometria Ultravioleta , Tioglicolatos/químicaRESUMO
Performance qualification (PQ) of taste sensing systems is mandatory for their use in pharmaceutical industry. According to ICH Q2 (R1) and a recent adaptation for taste sensing systems, non-specificity, log-linear relationships between the concentration of analytes and the sensor signal as well as a repeatability with relative standard deviation (RSD) values <4% were defined as basic requirements to pass a PQ. In the present work, the αAstree taste sensing system led to a successful PQ procedure by the use of recent sensor batches for pharmaceutical applications (sensor set #2) and a modified measurement protocol. Log-linear relationships between concentration and responses of each sensor were investigated for different bitter tasting active pharmaceutical ingredients (APIs). Using the new protocol, RSD values <2.1% were obtained in the repeatability study. Applying the visual evaluation approach, detection and quantitation limit could be determined for caffeine citrate with every sensor (LOD 0.05-0.5 mM, LOQ: 0.1-0.5 mM). In addition, the sensor set marketed for food applications (sensor set #5) was proven to show beneficial effects regarding the log-linear relationship between the concentration of quinine hydrochloride and the sensor signal. By the use of our proposed protocol, it is possible to implement the αAstree taste sensing system as a tool to assure quality control in the pharmaceutical industry.
Assuntos
Técnicas Biossensoriais/métodos , Indústria Farmacêutica/métodos , Eletrônica/métodos , Paladar/fisiologia , Tecnologia Farmacêutica/métodos , Língua/fisiologia , Técnicas Biossensoriais/instrumentação , Cafeína/química , Citratos/química , Indústria Farmacêutica/instrumentação , Eletrônica/instrumentação , Quinina/química , Tecnologia Farmacêutica/instrumentaçãoRESUMO
BACKGROUND: Thrombelastography (TEG) provides an effective and convenient means of whole blood coagulation monitoring. TEG evaluates the elastic properties of whole blood and provides a global assessment of hemostatic function. Previous studies performed TEG on native blood sample, but no data are available with citrated samples in healthy pregnant women at term. The aim of this study was to investigate the effect of pregnancy on coagulation assessed by TEG and establish normal ranges of TEG values in pregnant women at term comparing them with healthy non pregnant young women. METHODS: We enrolled pregnant women at term undergoing elective cesarean section or labour induction (PREG group) and healthy non-pregnant women (CTRL group). Women with fever or inflammatory syndrome, defined as C-reactive protein (CRP) >5 mg/L and with a platelet count <150.000/mm(3) have been excluded. For each women hemochrome and standard coagulation test were assessed. At the same time we performed a thrombelastographic test with Hemoscope TEG(®) after sample recalcification without using any activator. RESULTS: One hundred thirty patients were studied, 65 for each group. There were no differences between groups regarding demographic data. Hemoglobin, platelet count, International Normalized Ratio and Activated Partial Thromboplastin Time Ratio were lower and fibrinogen was higher in PREG group. All TEG parameters resulted as being significantly different between the groups with a hypercoagulable pattern in PREG group compared to CTRL group. CONCLUSION: The main findings of this study confirm the hypercoagulability status of pregnant women at term. This coagulation pattern is well represented by thrombelastographic trace obtained by recalcified citrate blood sample.
Assuntos
Testes de Coagulação Sanguínea/métodos , Citratos/química , Gravidez/sangue , Tromboelastografia/métodos , Adulto , Feminino , Tempo de Lise do Coágulo de Fibrina , Fibrinogênio/análise , Humanos , Coeficiente Internacional Normatizado , Tempo de Tromboplastina ParcialRESUMO
Sodium citrate (SC) and low temperatures between 7 and 5 degrees C are effective in suppressing aggregation of proteins and may be beneficial to be included during a purification process. In this work, we analyzed the application of dual salt system, ammonium sulfate (AS) and SC on binding and elution conditions of recombinant hSCOMT on typical HIC sorbents. Specifically in butyl and octyl supports, the use of, respectively, 300 mM AS/200 mM SC and 25 mM AS/25 mM SC in the loading buffer resulted in complete binding of COMT. Elution was obtained by decreasing the ionic strength to 0 M of salt. For the delineate goal, it also favorably increased the support chain length while a consequent decrease in the dual ionic strength was observed for hSCOMT retention. In the presence of dual salt systems octyl media exhibited classic HIC behavior, good protein selectivity, an excellent purification factor and reduced denaturation effects of hSCOMT observed with higher salt concentrations. Also the inclusion of temperature control during the elution step appears to be advantageous for greater activity recovery without enzyme aggregation. In fact, these results could allow the prediction of most stabilizing conditions for this termolabile enzyme on the chromatographic stage, regarding salt types and therefore effectiveness to improve HIC selectivity and desirable purity on the target fractions.
Assuntos
Catecol O-Metiltransferase/isolamento & purificação , Cromatografia Líquida/métodos , Sulfato de Amônio/química , Catecol O-Metiltransferase/química , Cromatografia Líquida/instrumentação , Citratos/química , Temperatura Baixa , Humanos , Interações Hidrofóbicas e Hidrofílicas , Concentração Osmolar , Ligação Proteica , Proteínas Recombinantes/química , Proteínas Recombinantes/isolamento & purificação , Citrato de SódioAssuntos
Citratos/química , Citratos/história , Indústria Farmacêutica/história , Carbonato de Lítio/química , Carbonato de Lítio/história , Psiquiatria/história , Adulto , Austrália , Transtorno Bipolar/tratamento farmacológico , Citratos/uso terapêutico , História do Século XX , Humanos , Carbonato de Lítio/uso terapêutico , Masculino , Pessoa de Meia-IdadeRESUMO
Acetyl Triethyl Citrate, Acetyl Tributyl Citrate, Acetyl Trihexyl Citrate, and Acetyl Trioctyl Citrate all function as plasticizers in cosmetics. Additionally, the Trihexyl and Trioctyl forms are described as skin-conditioning agents-emollients, although there are currently no reported uses of Acetyl Trihexyl Citrate or Acetyl Trioctyl Citrate. Acetyl Triethyl Citrate and Acetyl Tributyl Citrate are used in nail products at concentrations up to 7%. Recognizing that there are no reported uses of Acetyl Trihexyl or Trioctyl Citrate, if they were to be used in the future, their concentration of use is expected to be no higher than that reported for Acetyl Triethyl and Tributyl Citrate. These ingredients were sufficiently similar in structure that safety test data on one were considered applicable to all. Approximately 99% of orally administered Acetyl Tributyl Citrate is excreted-intermediate metabolites include acetyl citrate, monobutyl citrate, acetyl monobutyl citrate, dibutyl citrate, and acetyl dibutyl citrate. In acute, short-term, subchronic, and chronic feeding studies, these ingredients were relatively nontoxic. Differences from controls were either not statistically significant or not related to any organ toxicity. Ocular exposures produced moderate reactions that cleared by 48 hours after instillation. Dermal application was not toxic in rabbits. In a guinea pig maximization test, Acetyl Triethyl Citrate was a sensitizer whereas Acetyl Tributyl Citrate was not. Limited clinical testing of Acetyl Triethyl Citrate and Acetyl Tributyl Citrate was negative for both skin irritation and sensitization. These clinical data were considered more relevant than the guinea pig maximization data, suggesting to the Cosmetic Ingredient Review Expert Panel that none of these ingredients would be a sensitizer. Physiologic effects noted with intravenous delivery of Acetyl Triethyl Citrate or Acetyl Tributyl Citrate include dose-related decreases in blood pressure and intestinal muscular spasms. These ingredients were not genotoxic in bacterial or mammalian test systems. No significant differences in tumor induction (lymphomas) were noted in rats fed Acetyl Tributyl Citrate for 2 year. Acetyl Tributyl Citrate was not a developmental or reproductive toxicant in studies in mice and rats. Based on all the available data, these ingredients were considered safe as used in cosmetics.
Assuntos
Citratos/efeitos adversos , Citratos/química , Qualidade de Produtos para o Consumidor , Cosméticos/química , Plastificantes/química , Animais , Citratos/administração & dosagem , Ensaios Clínicos como Assunto , Cosméticos/administração & dosagem , Cosméticos/efeitos adversos , Relação Dose-Resposta a Droga , Vias de Administração de Medicamentos , Emolientes/administração & dosagem , Emolientes/efeitos adversos , Emolientes/química , Humanos , Plastificantes/administração & dosagem , Plastificantes/efeitos adversos , Testes de ToxicidadeAssuntos
Cromatografia de Afinidade/métodos , Fibronectinas/isolamento & purificação , Plasma/química , Adulto , Western Blotting , Adesão Celular/efeitos dos fármacos , Células Cultivadas , Fracionamento Químico , Cromatografia de Afinidade/economia , Citratos/química , Eletroforese , Endotélio Vascular/citologia , Fibronectinas/química , Fibronectinas/farmacologia , Gelatina/química , Humanos , Óleos de Plantas/química , Reprodutibilidade dos Testes , Cloreto de Sódio/química , Citrato de Sódio , Óleo de Girassol , Compostos de Tosil/química , Ureia/químicaRESUMO
Four commercially available non-particulate antacid preparations were titrated against 1M hydrochloric acid to assess buffering capacity as compared to 30 ml 0.3M sodium citrate solution. All antacids were used in the manufacturers "unit dose". All antacids tested demonstrated some in vitro buffering capacity, and "Eno" (Reckitt and Colman) had a buffering capacity similar to that of sodium citrate. The retail cost per unit dose was established for each proprietary antacid and for sodium citrate. It was concluded that while proprietary antacids are cheaper per dose than sodium citrate, preparations differ in their acid-neutralising capacity.