Complete genome sequence of Stellaria aquatica virus B, a novel polerovirus that infects Stellaria aquatica.

The complete genome sequence of Stellaria aquatica virus B (StAVB), a new member of the genus Polerovirus that infects Stellaria aquatica, was determined using high-throughput RNA sequencing with confirmation by Sanger sequencing. The complete StAVB genome (GenBank accession no. OP389993) is 5,900 nucleotide (nt) long with seven open reading frames (ORF0-5 and ORF3a) that encode putative proteins (P0-P5 and P3a) in a similar configuration to that of other typical poleroviruses. Pairwise sequence comparisons with other poleroviruses showed 38-50% nt sequence identity in the complete genome and 13-24%, 36-45%, 7-68%, and 6-50% amino acid sequence identity in (aa), for the P0, P1-2, P3, and P4 protein, respectively. These data, together with the results of phylogenetic analysis, indicate that StAVB should be classified as a new member of the genus Polerovirus, family Solemoviridae.

The complete genome sequence of Stellaria aquatica virus A, a new member of the genus Alphacarmovirus, family Tombusviridae.

A new member of the genus Alphacarmovirus was detected in Stellaria aquatica using high-throughput RNA sequencing analysis. The complete genome sequence of this new virus isolate, tentatively named "Stellaria aquatica virus A" (StAV-A), comprises 4,017 nucleotides with five predicted open reading frames (ORFs) and has a typical alphacarmovirus genome organization. Pairwise comparison of StAV-A with selected members of family Tombusviridae showed 44-58%, 32-64%, and 19-49% sequence identity for the overall nucleotide sequence, polymerase, and coat protein, respectively. Phylogenetic analysis of polymerase sequences places StAV-A alongside other members of the genus Alphacarmovirus in the family Tombusviridae.

Complete genome sequence of pueraria virus A, a new member of the genus Caulimovirus.

The complete genome sequence of a new caulimovirus in Pueraria montana was determined using high-throughput sequencing. The 7,572 nucleotide genome of pueraria virus A (PVA) contains genes that encode a movement protein, an aphid transmission factor, a virion-associated protein, a coat protein, a protease + reverse transcriptase + ribonuclease H, and a transactivator/viroplasmin protein, as well as two intergenic regions, which are all common features of members of the genus Caulimovirus. A sequence alignment revealed that the complete genome of PVA shares 66.82% nucleotide sequence identity with strawberry vein banding virus (GenBank accession no. KX249738.1). The results of phylogenetic analysis and the observation that the nucleotide sequence of the polymerase coding region differed by more than 20% indicated that PVA is a member of a new species the genus Caulimovirus, family Caulimoviridae.

Complete genome sequence of cnidium closterovirus 1, a novel member of the genus Closterovirus infecting Cnidium officinale.

The genome of a novel virus identified in Cnidium officinale is composed of a monopartite ssRNA of 16,755 nucleotides that shares 68.73% (query coverage, 20%) sequence identity with carrot yellow leaf virus (CYLV, accession no. FJ869862.1). It contains 11 putative open reading frames and has an organization typical of closteroviruses. It shares 30-50% nucleotide sequence identity with other closteroviruses. The heat shock protein 70-like protein (HSP70), putative RNA-dependent RNA polymerase (RdRp), and coat protein (CP) show 39-66%, 16-60%, and 24-41% amino acid sequence identity, respectively, to the homologous proteins of previously identified closteroviruses. Molecular and HSP70-based phylogenetic analysis of the genome and encoded protein sequences suggested that this virus is a novel member of the genus Closterovirus in the family Closteroviridae, which we have tentatively named "cnidium closterovirus 1" (CnClV1).

Complete genome sequence of Codonopsis torradovirus A, a novel torradovirus infecting Codonopsis lanceolata in South Korea.

We herein present the complete genome sequence of codonopsis torradovirus A (CoTVA), which was isolated from Codonopsis lanceolata (deodeok) in Gangwon-do, South Korea. The CoTVA genome contains two positive-sense RNA segments, namely RNA1 (6922 nucleotides), which encodes a predicted polyprotein, and RNA2 (4613 nucleotides), which encodes a movement protein and coat proteins (CPs). The proteinase-polymerase (Pro-Pol) and CP amino acid sequences were 75% and 54% identical, respectively, to those of motherwort yellow mosaic virus. Pairwise comparisons of the Pro-Pol and CP sequences revealed that the virus described in this study should be considered a member of a new torradovirus species. Phylogenetic analysis of the Pro-Pol sequence encoded by RNA1 and the CP region encoded by RNA2 indicated that CoTVA is a new member of the genus Torradovirus in the family Secoviridae. CoTVA is the first torradovirus detected in Codonopsis lanceolata.