Asunto(s)
Bioterrorismo/tendencias , Subtipo H5N1 del Virus de la Influenza A/genética , Subtipo H5N1 del Virus de la Influenza A/patogenicidad , Gripe Humana/transmisión , Gripe Humana/virología , Animales , Bioterrorismo/prevención & control , Humanos , Subtipo H5N1 del Virus de la Influenza A/fisiología , Salud Pública , Medición de Riesgo , Zoonosis/transmisión , Zoonosis/virologíaRESUMEN
Nonpigmented Yersinia pestis (pgm) strains are defective in scavenging host iron and have been used in live-attenuated vaccines to combat plague epidemics. Recently, a Y. pestis pgm strain was isolated from a researcher with hereditary hemochromatosis who died from laboratory-acquired plague. We used hemojuvelin-knockout (Hjv(-/-)) mice to examine whether iron-storage disease restores the virulence defects of nonpigmented Y. pestis. Unlike wild-type mice, Hjv(-/-) mice developed lethal plague when challenged with Y. pestis pgm strains. Immunization of Hjv(-/-) mice with a subunit vaccine that blocks Y. pestis type III secretion generated protection against plague. Thus, individuals with hereditary hemochromatosis may be protected with subunit vaccines but should not be exposed to live-attenuated plague vaccines.
Asunto(s)
Hemocromatosis/complicaciones , Vacuna contra la Peste/administración & dosificación , Peste/prevención & control , Yersinia pestis/patogenicidad , Animales , Femenino , Proteínas Ligadas a GPI , Hemocromatosis/genética , Proteína de la Hemocromatosis , Hígado/microbiología , Hígado/patología , Proteínas de la Membrana/genética , Ratones , Ratones de la Cepa 129 , Ratones Noqueados , Viabilidad Microbiana , Peste/genética , Peste/inmunología , Bazo/microbiología , Bazo/patología , Vacunas Atenuadas/administración & dosificación , Vacunas de Subunidad/administración & dosificación , Virulencia , Yersinia pestis/inmunologíaRESUMEN
To support increased research in the USA aimed at discovering products and technologies to combat infectious diseases, significant investment has been made to develop the appropriate laboratory infrastructure where these investigations can be conducted safely and securely. A key element to the success of this effort is the integration of these laboratories into their surrounding communities.