A review on recent advances in the applications of composite Fe3O4 magnetic nanoparticles in the food industry.

Fe3O4 magnetic nanoparticles (MNPs) have attracted tremendous attention due to their superparamagnetic properties, large specific surface area, high biocompatibility, non-toxicity, large-scale production, and recyclability. More importantly, numerous hydroxyl groups (-OH) on the surface of Fe3O4 MNPs can provide coupling sites for various modifiers, forming versatile nanocomposites for applications in the energy, biomedicine, and environmental fields. With the development of science and technology, the potential of nanotechnology in the food industry has also gradually become prominent. However, the application of composite Fe3O4 MNPs in the food industry has not been systematically summarized. Herein, this article reviews composite Fe3O4 MNPs, including their properties, modifications, and physical functions, as well as their applications in the entire food industry from production to processing, storage, and detection. This review lays a solid foundation for promoting food innovation and improving food quality and safety.

Homer1a protects against neuronal injury via PI3K/AKT/mTOR signaling pathway.

Purpose: Homer1a is a member of the post-synaptic density protein family that plays an important role in neuronal synaptic activity and is extensively involved in neurological disorders. The aim of this study is to investigate the role of Homer1a in modulating neuronal survival using an in vitro traumatic neuronal injury model.Materials and methods: Neurons were extracted from rats and identifited. Then, the cells were treated with Homerla overexpression or interference vectors. Western blot was performed to evaluate the expression of Homerla, apoptosis-related proteins(caspase3, caspase8, caspase9, Fasl, Bax, and p53), autophagy-related proteins (LC3ll and Beclin1), and the activiation of PI3K/AKT/mTOM pathway. In addition, the cell viability and apoptosis rate were measured. Results: After transfection with overexpression or interference vectors, the mRNA and protein expression of Homer1a increased or decreased significantly, respectively. Upregulation of Homer1a significantly alleviated apoptosis and enhanced cell viability and autophagy after traumatic neuronal injury. Homer1a overexpression also significantly decreased the expression of the pro-apoptosis proteins caspase 3, caspase 8, caspase 9, Fasl, Bax, and p53 in neurons. Furthermore, neuron autophagy was increased after traumatic neuronal injury as demonstrated by the greater accumulation of autophagosomes and higher expression of LC3II and Beclin1 induced by Homer1a overexpression. In addition, Homer1a overexpression inhibited the activation of PI3K/AKT/mTOR signaling. Conclusion: These findings indicated that Homer1a potentially protects neurons from traumatic injury by regulating apoptosis and autophagy via the caspase and PI3K/AKT/mTOR signaling pathways and may be an effective intervention target in traumatic brain injury.

Invasiveness-triggered state transition in malignant melanoma cells.

Cancer cells are considered to have high morphological heterogeneity in human melanoma tissue. Here, we report that epithelial cancer cells are dominant in different development stages of human melanoma tissues. The cellular and molecular mechanisms that maintain melanoma cells in the epithelial state are further investigated in the A2058 cell line. We find that micropore (8 µm) transwell invasion, but not superficial migration in the scratch assay, can induce remarkable morphological changes between epithelial and mesenchymal melanoma cells within 4 days. The morphological switch is associated with dynamic changes of epithelial-mesenchymal transition (EMT) hallmarks E-cadherin and vimentin. Further immunoflurencent staining and co-immunoprecipitation assay showed the uncoupling of the M3 muscarinic acetylcholine receptor (mAChR) and the p75 neurotrophin receptor (p75NTR) in epithelial melanoma cells. Specific knockdown of M3 mAChR by small interfering RNA (siRNA) significantly abrogates the transition of spindle-shaped mesenchymal cells to epithelial cells. Collectively, we report a cellular model of invasiveness-triggered state transition (ITST) in which melanoma cell invasion can induce morphological changes between epithelial and mesenchymal cells. ITST is one of the biological basis for maintaining metastatic melanoma cells in the epithelial state. Furthermore, M3 mAChR receptor-mediated ITST provides a novel therapeutic strategy to inhibit the development of malignant melanoma.

Experimental investigation of nitric oxide removal from flue gas using hexamminecobalt(II) solution scrubbing in a pilot-scale facility.

An experimental investigation of operational parameters, including liquid/gas ratio (L/G), inlet nitric oxide (NO) concentration, reaction temperature, and pH value of absorbing agent, on NO removal efficiency with hexamminecobalt(II) solution scrubbing was conducted on a pilot-scale facility to search optimal operation conditions. The experimental results show that NO removal efficiency increased with the pH value of hexamminecobalt solution, while the improving rate dropped gradually. When the reaction temperature increased, the NO removal efficiency increased first and then decreased. At the same time, NO removal efficiency increased with the increasing of L/G and hexamminecobalt concentration, while the removal efficiency did not change much at low NO concentration. The pH of 10.4 and L/G of 16 L/m3 were close to the optimal operation conditions, and the scrubbing temperature fell within a reasonable operation temperature. The experimental results can be used as a reference for the design and operation of scaled-up industrial devices.

Molecular docking to understand the metabolic behavior of GNF-351 by CYP3A4 and its potential drug-drug interaction with ketoconazole.

GNF-351 is a candidate drug used to treat some diseases through antagonizing aryl hydrocarbon receptor. In the present study, molecular docking method was employed to understand the interaction between ketoconazole and GNF-351. The structure of cytochrome P450 (CYP) 3A4 was obtained from protein data bank, and 2-dimensional structure of GNF-351 with standard bond lengths and angles was drawn using chemdraw software. 30 possible binding orientations was generated and docked into the X-ray crystallographic structure of human CYP3A4. The predicted binding mode of GNF-351 into CYP3A4 appeared to adopt an orientation with interactions between their flat aromatic rings and Phe 302 and Phe 304. The comparison for the binding of GNF-351 and ketoconazole into the activity cavity indicated that they exhibited similar distance towards heme, indicating the potential interaction between GNF-351 and ketoconazole. These data remind us the necessary monitoring when future utilization between GNF-351 and ketoconazole.

Classification and comparison of municipal solid waste based on thermochemical characteristics.

UNLABELLED: Municipal solid waste (MSW) has been normally sorted into six categories, namely, food residue, wood waste, paper textiles, plastics, and rubber In each category, materials could be classified further into subgroups. Based on proximate and ultimate analysis and heating value, statistical methods such as analysis of variance (ANOVA) and cluster analysis were applied to analyze the characteristics of MSW in every subgroup and to try to distinguish their relative properties. The chemical characteristics analysis of MSW showed that polyethylene (PE), polypropylene (PP), and polystyrene (PS) had the highest volatile matter content, with almost no ash and fixed carbon, while polyethylene terephthalate (PET) had high carbon content but low hydrogen content. Bones and vegetables had the highest ash content, while nutshells and rubber had the highest fixed carbon content. Paper and starch food had the highest oxygen content, and wool and bones had the highest nitrogen and sulfur content. Polyvinyl chloride (PVC) had the highest chlorine content at about 55%. PE, PP and PS had the highest heating value, followed by chemical products such as rubber and chemical fiber. Conversely, paper, vegetables and bones had the lowest heating value. The results of cluster analysis of MSW components showed that fruit peel, weeds, wood, bamboo, leaves and nutshells could be classified as the lignocellulose category; starch food, cotton, toilet paper, printing paper and cardboard could be classified as the glucose monomer category; wood and chemical fiber could be classified as the high nitrogen and sulfur category; and PE, PP, and PS could be cluster as the polyolefin category. IMPLICATIONS: The yield of municipal solid waste (MSW) is constantly increasing and waste to energy (WTE) has been used extensively all over the world. During the processes of incineration, pyrolysis, or gasification, the impact of physical and chemical properties of MSW is of great significance. However, the traditional classification of MSW is too general to provide more detailed information in many investigations. It is necessary to perform the investigation of characteristics of combustible MSW to distinguish different categories of MSW and find out their subclassification.

Comprehensive compositional analysis of liquid organic product prepared by industrialized hydrothermal cracking of biomass waste and its potential application as fertilizer.

Hydrothermal cracking involves the conversion of organic waste into efficient fertilizer through hydrolysis at temperatures ranging from 180 to 220 °C and pressures of 1.5 to 2.45 MPa, which offers significant advantages in shortening the production cycle, enhancing efficiency, and decomposing antibiotics. As a result, it holds immense practical value for promoting organic fertilizer manufacturing processes globally. The products derived from hydrothermal cracking can be categorized into solid and liquid components. Extensive research has focused on the composition and use of solids, while studies on liquids have mainly examined basic characteristics. The study aimed to comprehensively analyze the components in liquid products prepared through hydrothermal cracking and evaluate their suitability as liquid fertilizers. Specifically, we employed rigorous analytical techniques to accurately identify and quantify medium and trace elements, organic acids, amino acids, and plant growth regulators. Furthermore, we carried out a planting experiment to assess the yield and soil changes following the application of liquid products in maize cultivation. The experimental data revealed that the liquid product contained abundant medium and trace elements, along with 6.22 g/L free amino acids and 9.22 g/L organic acids. It is noteworthy that this liquid product contained 1.22 × 105 pg/mL ABA, 6.26 × 103 pg/mL IAA, 1.07 × 102 pg/mL IBA, and 3.60 × 10-2 pg/mL GA3. The utilization of this liquid product has the potential to enhance the disease resistance of maize crops and promote the accumulation of nitrate nitrogen in the soil. By understanding the composition of liquid products via hydrothermal cracking, valuable insights can be gained into their potential benefits for agricultural and ecological applications.

Silver Nanocomposites with Enhanced Shelf-Life for Fruit and Vegetable Preservation: Mechanisms, Advances, and Prospects.

Reducing fruit and vegetable waste and maintaining quality has become challenging for everyone. Nanotechnology is a new and intriguing technology that is currently being implemented in fruit and vegetable preservation. Silver nanomaterials provide superior antibacterial qualities, biodegradability, and biocompatibility, which expands their potential applications in fruit and vegetable preservation. Silver nanomaterials include silver nanocomposites and Ag-MOF, of which silver nanocomposites are mainly composed of silver nanoparticles. Notably, not all kinds of silver nanoparticles utilized in the preservation of fruits and vegetables are thoroughly described. Therefore, the synthesis, mechanism of action, and advancements in research on silver nanocomposites for fruit and vegetable preservation were discussed in this study.

Characteristics of industrialized hydrothermal cracking solid organic fertilizer and its effects on fresh corn growth.

Traditional methods of producing organic fertilizers result in significant nutrient loss and greenhouse gas emissions, making it challenging to align with sustainable development and the achievement of net-zero emissions goals. Hydrothermal cracking, as a novel clean technology for the utilization of organic waste into fertilizer, has been extensively studied and refined in laboratory settings, but its large-scale industrial evaluation remains limited. This study investigates the properties and field application of hydrothermal cracking solid organic fertilizer (HCSOF) produced at a pilot scale with an annual output of 10,000 tons. The results indicate that the organic matter content and total nutrient content (TN + P2O5 + K2O) of HCSOF reached 50.6 % and 5.46 %, respectively, which are 20.6 % and 1.46 % higher than the standards for organic fertilizers in China. Additionally, contaminants such as pathogens and antibiotics in the product were completely eliminated. Elemental analysis and pore size distribution highlighted the unique adsorptive attributes of HCSOF, which showed significant effect in reducing soil ammonium nitrogen. Results from field trials indicate that the complete substitution of chemical fertilizers with HCSOF did not reduce corn yield, which remained at 9.03 t/ha. Particularly, compared to the exclusive use of chemical fertilizers, HCSOF treatments resulted in a 7.03 % and 4.70 % decrease in fresh corn lodging and disease incidence, respectively. Antibacterial tests further confirmed its ability to counter pathogens. This study provides robust evidence for scaling up hydrothermal cracking fertilizer production from laboratory to industrial levels. Future research should focus on multi-batch sampling and extended field experiments.

Towards Negative Emissions: Hydrothermal Carbonization of Biomass for Sustainable Carbon Materials.

The contemporary production of carbon materials heavily relies on fossil fuels, contributing significantly to the greenhouse effect. Biomass is a carbon-neutral resource whose organic carbon is formed from atmospheric CO2. Employing biomass as a precursor for synthetic carbon materials can fix atmospheric CO2 into solid materials, achieving negative carbon emissions. Hydrothermal carbonization (HTC) presents an attractive method for converting biomass into carbon materials, by which biomass can be transformed into materials with favorable properties in a distinct hydrothermal environment, and these carbon materials have made extensive progress in many fields. However, the HTC of biomass is a complex and interdisciplinary problem, involving simultaneously the physical properties of the underlying biomass and sub/supercritical water, the chemical mechanisms of hydrothermal synthesis, diverse applications of resulting carbon materials, and the sustainability of the entire technological routes. This review starts with the analysis of biomass composition and distinctive characteristics of the hydrothermal environment. Then, the factors influencing the HTC of biomass, the reaction mechanism, and the properties of resulting carbon materials are discussed in depth, especially the different formation mechanisms of primary and secondary hydrochars. Furthermore, the application and sustainability of biomass-derived carbon materials are summarized, and some insights into future directions are provided.

Comparison of Chemiluminescence Enzyme Immunoassay (Cl-ELISA) with Colorimetric Enzyme Immunoassay (Co-ELISA) for Imidacloprid Detection in Vegetables.

Imidacloprid is one of the most commonly used insecticides for managing pests, thus, improving the quality and yield of vegetables. The abuse/misuse of imidacloprid contaminates the environment and threatens human health. To reduce the risk, a colorimetric enzyme-linked immunoassay assay (Co-ELISA) and chemiluminescence enzyme-linked immunoassay assay (Cl-ELISA) were established to detect imidacloprid residues in vegetables. The linear range of Co-ELISA ranged between 1.56 µg/L and 200 µg/L with a limit of detection (LOD) of 1.56 µg/L. The values for Cl-ELISA were 0.19 µg/L to 25 µg/L with an LOD of 0.19 µg/L, which are lower than those of Co-ELISA. Fortifying Chinese cabbage, cucumber, and zucchini with imidacloprid at 10, 50, and 100 µg/L yielded recoveries between 81.7 and 117.6% for Co-ELISA and at 5, 10, and 20 µg/L yielded recoveries range from 69.7 to 120.6% for Cl-ELISA. These results indicate that Cl-ELISA has a high sensitivity and a rapid detection time, saving cost (antigen and antibody concentrations) and serving as a more efficient model for the rapid detection of imidacloprid residue.

Retraction of "Cu(II)-Containing Metal-Organic Framework with 1D Hexagonal Channels for Cyanosilylation Reaction and Anticancer Activity on Osteosarcoma Cells".

[This retracts the article DOI: 10.1021/acsomega.0c06270.].

Magnetic Composite Based on Carbon Nanotubes and Deep Eutectic Solvents: Preparation and Its Application for the Determination of Pyrethroids in Tea Drinks.

In this study, a novel composite material prepared by using deep eutectic solvent (tetrabutylammonium chloride-dodecanol, DES5) functionalized magnetic MWCNTs-ZIF-8 (MM/ZIF-8@DES5) was employed as an adsorbent for the magnetic solid-phase extraction of six pyrethroids from tea drinks. The characterization results show that MM/ZIF-8@DES5 possessed sufficient specific surface area and superparamagnetism, which could facilitate the rapid enrichment of pyrethroids from tea drink samples. The results of the optimization experiment indicated that DES5, which comprised tetrabutylammonium chloride and 1-dodecanol, was selected for the next experiment and that the adsorption properties of MM/ZIF-8@DES5 were higher than those of MM/ZIF-8 and M-MWCNTs. The validation results show that the method has a wide linear range (0.5-400 µg L-1, R2 ≥ 0.9905), low LOD (0.08-0.33 µg L-1), and good precision (intra-day RSD ≤ 5.6%, inter-day RSD ≤ 8.6%). The method was successfully applied to the determination of pyrethroids in three tea drink samples.

Decoupled temperature and pressure hydrothermal synthesis of carbon sub-micron spheres from cellulose.

The temperature and pressure of the hydrothermal process occurring in a batch reactor are typically coupled. Herein, we develop a decoupled temperature and pressure hydrothermal system that can heat the cellulose at a constant pressure, thus lowering the degradation temperature of cellulose significantly and enabling the fast production of carbon sub-micron spheres. Carbon sub-micron spheres can be produced without any isothermal time, much faster compared to the conventional hydrothermal process. High-pressure water can help to cleave the hydrogen bonds in cellulose and facilitate dehydration reactions, thus promoting cellulose carbonization at low temperatures. A life cycle assessment based on a conceptual biorefinery design reveals that this technology leads to a substantial reduction in carbon emissions when hydrochar replacing fuel or used for soil amendment. Overall, the decoupled temperature and pressure hydrothermal treatment in this study provides a promising method to produce sustainable carbon materials from cellulose with a carbon-negative effect.

Residue, Dissipation Pattern, and Dietary Risk Assessment of Imidacloprid in Chinese Chives.

The demand for Chinese chives is growing as they are also rich in vitamins, fiber, and sulfur nutrients. Chinese chives should be sprayed with imidacloprid to control pests and diseases to safeguard their yield and to meet the demands of East Asian consumers for Chinese chives. Overspraying of imidacloprid can lead to residues in Chinese chives, posing a severe risk to human health. To reduce the harmful effects of imidacloprid residues on humans, we investigated the imidacloprid dissipation pattern and the final residue on Chinese chives using the quick, easy, cheap, effective, rugged, and safe (QuEChERS) method combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Good linearity (R 2= 0.9988), accuracy (expressed as recovery % of 78.34-91.17%), precision [expressed as relative SDs (RSDs) of 0.48-6.43%], and sensitivity [a limit of quantification (LOQ) ≤ 8.07 × 104 mg/kg] were achieved. The dissipation dynamics were consistent with the first-order kinetics, with a half-life of 2.92 days. The final residual levels on Chinese chives were 0.00923-0.166 mg/kg, which is lower than the maximum residue limits (MRLs) of 1 mg/kg for imidacloprid on Chinese chives. A risk assessment index of <1 indicates that Chinese chives are safe for consumption.

Enzyme inhibition methods based on Au nanomaterials for rapid detection of organophosphorus pesticides in agricultural and environmental samples: A review.

Background: Organophosphorus pesticides (OPs), as insecticides or acaricides, are widely used in agricultural products to ensure agricultural production. However, widespread use of OPs leads to environmental contamination and significant negative consequences on biodiversity, food security, and water resources. Therefore, developing a sensitive and rapid method to determine OPs residues in different matrices is necessary. Originally, the enzyme inhibition methods are often used as preliminary screens of OPs in crops. Many studies on the characteristic of Au nanomaterials have constantly been emerging in the past decade. Combined with anisotropic Au nanomaterials, enzyme inhibition methods have the advantages of high sensitivity, durability, and high stability. Aim of Review: This review aims to summarize the principles and strategies of gold (Au) nanomaterials in enzyme inhibition methods, including colorimetric (dispersion, particle size of Au nanomaterials) and fluorometric (fluorescence energy transfer, internal filtration effect) detection, and electrochemical sensing system (shape of Au nanomaterials, Au nanomaterials combined with other nanomaterials). The application of enzyme inhibition in agricultural products and research progress was also outlined. Next, this review illustrates the advantages of Au nanomaterial-based enzyme inhibition methods compared with conventional enzyme inhibition methods. The detection limits and linear range of colorimetric and fluorometric detection and electrochemical biosensors have also been provided. At last, key perspectives, trends, gaps, and future research directions are proposed. Key Scientific Concepts of Review: Herein, we introduced the technology of enzyme inhibition method based on Au nanomaterials for onsite and infield rapid detection of organophosphorus pesticide.

A Competitive Assay Based on Dual-Mode Au@Pt-DNA Biosensors for On-Site Sensitive Determination of Carbendazim Fungicide in Agricultural Products.

Carbendazim (CBZ), a systemic, broad-spectrum benzimidazole fungicide, is widely used to control fungal diseases in agricultural products. Its residues might pose risks to human health and the environment. Therefore, it is warranted to establish a rapid and reliable method for its residual quantification. Herein, we proposed a competitive assay that combined aptamer (DNA) specific recognition and bimetallic nanozyme gold@platinum (Au@Pt) catalysis to trace the CBZ residue. The DNA was labeled onto bimetallic nanozyme Au@Pt surface to produce Au@Pt probes (Au@Pt-DNA). The magnetic Fe3O4 was functionalized with a complementary strand of DNA (C-DNA) to form Fe3O4 probes (Fe3O4-C-DNA). Subsequently, the CBZ and the Fe3O4 probes competitively react with Au@Pt probes to form two Au@Pt-DNA biosensors (Au@Pt-ssDNA-CBZ and Au@Pt-dsDNA-Fe3O4). The Au@Pt-ssDNA-CBZ biosensor was designed for qualitative analysis through a naked-eye visualization strategy in the presence of CBZ. Meanwhile, Au@Pt-dsDNA-Fe3O4 biosensor was developed to quantitatively analyze CBZ using a multifunctional microplate reader. A competitive assay based on the dual-mode Au@Pt-DNA biosensors was established for onsite sensitive determination of CBZ. The limit of detection (LOD) and recoveries of the developed assay were 0.038 ng/mg and 71.88-110.11%, with relative standard deviations (RSDs) ranging between 3.15 and 10.91%. The assay demonstrated a good correlation with data acquired from liquid chromatography coupled with mass spectrometry/mass spectrometry analysis. In summary, the proposed competitive assay based on dual-mode Au@Pt-DNA biosensors might have a great potential for onsite sensitive detection of pesticides in agro-products.

miR-9-5p Mediates ABCC1 to Elevate the Sensitivity of Glioma Cells to Temozolomide.

Chemotherapy combined with surgery is an important clinical treatment for glioma, but endogenous or acquired temozolomide (TMZ) resistance can lead to poor prognosis. microRNA (miR)-9-5p acts in biological function of glioma, but the drug resistance of miR-9-5p in glioma is under exploration. The study intended to test the molecular mechanism of miR-9-5p in glioma cells. MTT assay was applied to investigate the chemosensitivity enhancement of miR-9-5p on TMZ in glioma cells U87-TMZ and U251-TMZ, and in vivo experiments confirmed its role on tumor growth in nude mice. The results of double luciferase reporter gene assay, qRT-PCR and WB indicated that miR-9-5p directly targeted ABCC1 (ATP binding cassette subfamily C member 1) to reduce its expressions. MTT and flow cytometry indicated that elevation of miR-9-5p or down-regulation of ABCC1 could inhibit proliferation-induced apoptosis of drug-resistant cells, and the decrease of miR-9-5p could reverse the reduction of ABCC1 on proliferation-induced apoptosis of drug-resistant cells. In vivo experiments showed that miR-9-5p could promote the anti-tumor role of TMZ. To sum up, the increase of miR-9-5p directly targets ABCC1 and may make glioma cells sensitive to TMZ.

Thermal behaviour and kinetic study of co-pyrolysis of microalgae with different plastics.

The coexistence of plastics and microalgae in the ocean has brought great challenges to the environment. Therefore, co-pyrolysis of microalgae Dunaliella salina (DS) and typical plastics (polypropylene (PP), polystyrene (PS), polyethylene terephthalate (PET), and polyvinyl chloride (PVC)) were investigated using thermogravimetric analyzer with Fourier transform infrared spectrometer. The results showed that the coating effect of the molten plastics promoted the pyrolysis of DS. The solid residue amounts of DS-PP, DS-PS, and DS-PET blends were reduced by 1.55 wt%, 1.39 wt%, 1.69 wt%, respectively, as a result of the hydrogenation reaction between the unsaturated products generated by plastics and biochar. While for DS-PVC, attributed to the physical and chemical effects during the co-pyrolysis process, the solid residue was increased by 1.36 wt%. For the other three blends, the solid residues were reduced due to the hydrogenation reaction between the unsaturated products generated by plastics and biochar. FTIR analysis of gaseous products indicated the total CO2 production increased significantly for DS-PET. Besides, the alkyls generated by DS reacted with HCl during DS-PVC co-pyrolysis, the resulting products were then fixed in biochar. Kinetic results suggested that due to the co-pyrolysis with DS, the activation energies of PP, PS, and PET were reduced by 1/2, 1/3, and 3/4, respectively, and this value for PVC in its second stage was reduced by 1/4. Our results indicated the advantage to co-pyrolyze the microalgae and marine plastics.

Cu(II)-Containing Metal-Organic Framework with 1D Hexagonal Channels for Cyanosilylation Reaction and Anticancer Activity on Osteosarcoma Cells.

A novel metal-organic framework (MOF) has been produced via Cu(NO3)2·6H2O reaction with 3-(1H-tetrazol-5-yl)pyridine (HL) in water, and its chemical formula is {[(Cu(L)2(H2O)2](H2O)8) n } (1). Due to its high density of coordinately unsaturated sites along with large one-dimensional (1D) hexagonal channels, the activated complex 1 (1a) was explored as the solvent-free heterogeneous catalyst for cyanosilylation under mind conditions. The inhibitory function of compound 1a against the survival rate of OS-732 osteosarcoma cells was evaluated via Cell Counting Kit-8 (CCK-8) detection kit. Furthermore, the Annexin V-FITC/PI detection kit and the active oxygen (ROS) detection kit was carried out to determine the cell apoptosis levels and the ROS accumulation in OS-732 osteosarcoma cells after treatment by compound 1a.