RESUMO
The diagnosis of bland-looking spindle cell lesions of the breast is often challenging because there is a close morphological and immunohistochemical overlap among the different entities. The present review will discuss reactive spindle cell nodule/exuberant scar, nodular fasciitis, inflammatory pseudotumor, myofibroblastoma (classic type), lipomatous myofibroblastoma, palisaded myofibroblastoma, benign fibroblastic spindle cell tumor, spindle cell lipoma, fibroma, leiomyoma, solitary fibrous tumor, myxoma, schwannoma/neurofibroma, desmoid-type fibromatosis, dermatofibrosarcoma protuberans, low-grade fibromatosis-like spindle cell carcinoma, inflammatory myofibroblastic tumor and low-grade myofibroblastic sarcoma arising in the breast parenchyma. The pathologist should be aware of each single lesion to achieve a correct diagnosis to ensure patient a correct prognostic information and therapy. Accordingly representative illustrations and morphological/immunohistochemical diagnostic clues will be provided.
Assuntos
Neoplasias da Mama/diagnóstico , Carcinoma/diagnóstico , Dermatofibrossarcoma/diagnóstico , Fasciite/diagnóstico , Fibrossarcoma/diagnóstico , Neoplasias de Tecido Muscular/patologia , Neoplasias Cutâneas/diagnóstico , Mama/patologia , Neoplasias da Mama/patologia , Carcinoma/patologia , Dermatofibrossarcoma/patologia , Diagnóstico Diferencial , Fasciite/patologia , Feminino , Fibrossarcoma/patologia , Humanos , Neoplasias de Tecido Muscular/diagnóstico , Neoplasias Cutâneas/patologiaRESUMO
BACKGROUND: Oral challenges are the gold standard in food allergy diagnostic, but time-consuming. Aim of the study was to investigate the role of peanut- and hazelnut-component-specific IgE in the diagnostics of peanut and hazelnut allergy and to identify cutoff levels to make some challenges superfluous. METHODS: In a prospective and multicenter study, children with suspected peanut or hazelnut allergy underwent oral challenges. Specific IgE to peanut, hazelnut, and their components (Ara h 1, Ara h 2, Ara h 3, and Ara h 8, Cor a 1, Cor a 8, Cor a 9, and Cor a 14) were determined by ImmunoCAP-FEIA. RESULTS: A total of 210 children were challenged orally with peanut and 143 with hazelnut. 43% of the patients had a positive peanut and 31% a positive hazelnut challenge. With an area under the curve of 0.92 and 0.89, respectively, Ara h 2 and Cor a 14-specific IgE discriminated between allergic and tolerant children better than peanut- or hazelnut-specific IgE. For the first time, probability curves for peanut and hazelnut components have been calculated. A 90% probability for a positive peanut or hazelnut challenge was estimated for Ara h 2-specific IgE at 14.4 kU/l and for Cor a 14-specific IgE at 47.8 kU/l. A 95% probability could only be estimated for Ara h 2 at 42.2 kU/l. CONCLUSIONS: Ara h 2- and Cor a 14-specific IgE are useful to estimate the probability for a positive challenge outcome in the diagnostic work-up of peanut or hazelnut allergy making some food challenges superfluous.
Assuntos
Especificidade de Anticorpos , Arachis/efeitos adversos , Corylus/efeitos adversos , Hipersensibilidade Alimentar/diagnóstico , Hipersensibilidade Alimentar/imunologia , Imunoglobulina E/imunologia , Alérgenos , Antígenos de Plantas , Criança , Pré-Escolar , Comorbidade , Feminino , Humanos , Masculino , Hipersensibilidade a Amendoim/diagnóstico , Hipersensibilidade a Amendoim/imunologia , Estudos Prospectivos , Curva ROCRESUMO
Lymphoma of cheek is a rare ad uncommon disease, representing 2,5% of malignant lymphoma. The cause is unknown but there are a lot of risk factors such as Helicobacter pylori and Epstein Barr virus. Symptoms are aspecific and may be confused with otolaryngological benign diseases. We present a case of B cell lymphoma of the cheek, which presented with a history of a slowly growing swelling of 3 months duration, resistant to NSAIDs and antibiotic therapy. Biopsy of the mass led to diagnosis of lymphoma. Blood investigations, ultrasonography and CT scan helped to reach this result. This case report shows that an accurate clinical examination, a cytohistological and immune-histochemical diagnosis by fine-needle aspiration biopsy (FNAB) are fundamental to obtain a diagnosis and to decide therapy.
Assuntos
Bochecha/patologia , Linfoma de Células B/diagnóstico , Idoso , Biópsia por Agulha Fina , Feminino , Citometria de Fluxo , Humanos , Linfoma de Células B/patologia , Tomografia Computadorizada por Raios XRESUMO
Primary intestinal lymphoma is rare representing about 0.5% of all colonic malignancies. It can be classified into two principal categories: follicular B cell lymphomas and intestinal T cell lymphomas. Other intestinal diseases are very important such as immunoproliferative small intestinal disease (IPSID), a prelymphomatous process, and MALT lymphomas, caused by infection of Helicobacter pylori (H. Pylori). We present a 79-year-old male patient which presented with abdominal pain in the upper parts of abdomen of four months' duration, colic timpanists, tenderness, distention, weight loss. Sometimes the abdominal pain decreased expelling diarrheal dejections. Histological and immune-histochemical tests on bioptic piece helped to reach the diagnosis of lymphoma but only after histological investigation on operative piece was made the diagnosis of B-cell lymphoma. This case report shows that an accurate diagnosis, the evaluation of the extension and the presence of particular infections and/or co morbidities (H. Pylori positive, age, performance status) are fundamental to decide the therapeutic protocol.
Assuntos
Neoplasias Intestinais/patologia , Linfoma de Células B/patologia , Idoso , Antígenos CD/análise , Biópsia , Colectomia , Infecções por Helicobacter/complicações , Humanos , Neoplasias Intestinais/cirurgia , Linfoma de Células B/cirurgia , Masculino , Linfócitos T/imunologia , Tomografia Computadorizada por Raios XRESUMO
Malignant lateral cervical cysts can be related to metastatic tumors or rarely to primary thyroid carcinoma arising in branchial cleft cysts. This study evaluates the expression of thyroid-associated transcription factor-1 (TTF-1) and p63 in three branchial cleft cysts and in two primary thyroid papillary carcinoma of branchial cleft cysts. TTF-1 was negative in the nuclei of the lining epithelia of branchial cleft cysts, but positive in the adjacent normal thyroid tissue, while TTF-1 was positive in the nuclei of the lining epithelia and in the nuclei of the papillae and follicles in branchial cleft cysts with ectopic thyroid carcinoma. P63 was positive in the nuclei of the lining epithelia of branchial cleft cysts, but negative in the adjacent normal thyroid tissue. Papillary thyroid carcinoma of branchial cleft cysts displayed p63-positive foci. In conclusion, our results demonstrate that TTF-1 cannot distinguish between primary and metastatic tumors of branchial cleft cysts. The detection of p63 in papillary thyroid carcinomas of branchial cleft cysts could suggest that p63 contributes to the onset of this tumor. It is really important to evaluate if the case has a metastatic derivation or represents papillary thyroid carcinoma arising in ectopic thyroid tissue in a branchial cleft cyst.
Assuntos
Adenocarcinoma Papilar/metabolismo , Branquioma/metabolismo , Proteínas de Ligação a DNA/metabolismo , Proteínas de Membrana/metabolismo , Neoplasias Primárias Múltiplas/metabolismo , Neoplasias da Glândula Tireoide/metabolismo , Adenocarcinoma Papilar/patologia , Adulto , Biomarcadores Tumorais/metabolismo , Branquioma/patologia , Núcleo Celular/metabolismo , Núcleo Celular/patologia , Criança , Feminino , Técnica Indireta de Fluorescência para Anticorpo , Humanos , Técnicas Imunoenzimáticas , Masculino , Pessoa de Meia-Idade , Neoplasias Primárias Múltiplas/patologia , Estudos Retrospectivos , Neoplasias da Glândula Tireoide/patologia , Fatores de TranscriçãoRESUMO
A case of sigmoid-rectal endometriosis prompted the authors to focus attention on the question of indications for surgery and operative tactics in intestinal endometriosis. In this case, the presence of a pelvic mass which could not be cut away from the intestinal wall without the risk of perforation led to the performance of a low anterior resection of the rectum with mechanical stapler, a choice which was certainly radical but necessary in view of the impossibility of excluding the malignant nature of the mass either macroscopically or extemporaneously.
Assuntos
Endometriose/cirurgia , Doenças Retais/cirurgia , Reto/cirurgia , Doenças do Colo Sigmoide/cirurgia , Grampeadores Cirúrgicos , Adulto , Anastomose Cirúrgica , Colo/patologia , Colo/cirurgia , Endometriose/patologia , Feminino , Humanos , Doenças Retais/patologia , Reto/patologia , Doenças do Colo Sigmoide/patologiaRESUMO
The major advantage offered by the "double stapling technique" in the treatment of low rectum cancers is sphincter preservation while respecting oncological criteria of radicality. The authors report their experience in surgical treatment of rectal neoplasms, considering a 24 patient group observed between 1989 and 1991, when new staplers were available in Italy. They also present primary results obtained with modified Knight-Griffen technique evaluating a series of 10 patients with rectal cancers placed between 5 and 10 cm from the anal verge. The authors describe surgical procedures and, analyse the complication rate, report a 10% anastomotic leak with no clinical stenosis and 20% relapses. Showing a 2-year follow-up data, a 61.1% surviving actually free of disease in the all 24-patient group is referred. The authors conclude that double stapling technique, while safe and effective, should be always performed accordingly to oncological radicality, reminding that the aim of surgery is the treatment of cancer and sphincter saving should be considered secondary.
Assuntos
Adenocarcinoma/cirurgia , Neoplasias Retais/cirurgia , Grampeadores Cirúrgicos , Idoso , Idoso de 80 Anos ou mais , Anastomose Cirúrgica , Colo/cirurgia , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Reto/cirurgia , Fatores de TempoRESUMO
Glottic carcinoma, in the early stage, may benefit, with excellent results "quoad vitam atque quoad valitudinem", from various modes of treatment--radiotherapy, laser microsurgery and cordectomy being the most common--in definitive cure of the disease. Nevertheless, recurrence, in relation to treatment, oscillates between 4.3-24.1% with laser CO2 surgery, 5.5-32.4% for cordectomy and 5.3-34% for radiotherapy. Prognostic biological markers of recurrence remain to be elucidated, mainly due to the clinical differences in the subjects examined. The present study focused on patients with glottic T1a carcinoma treated with CO2 laser surgery in which correlation between histo-pathological aspects and expression of p53 protein on resection borders were confirmed by onset of local recurrence. Study population comprised 39 patients treated with CO2 laser surgery (January 1985-December 1991) in Clinical Division of Otorhinolarygology, University of Catania. Survival rate, free from recurrence, at 3 and 5 years for this patient group was 87.1% (34/39) and 82% (32/39), respectively. Disease-free survival at 3 and 5 years, was 86.6% in patients with positive resection margins for carcinoma and 87.5% and 79.1%, respectively, for patients with negative resection margins. Survival rate, free from local recurrence, in p53 positive patients, at 3 and 5 years was 78.9% and 68.4%, respectively. In p53 negative patients, survival, free from local recurrence, at 3 and 5 years, was 94.7%. Presence of overexpression of oncoprotein p53 on borders of resection with aspects of dysplasia of various degrees seems, therefore, a marker of high risk of tumour progression and recurrence.
Assuntos
Carcinoma de Células Escamosas/metabolismo , Glote , Neoplasias Laríngeas/metabolismo , Recidiva Local de Neoplasia/metabolismo , Proteína Supressora de Tumor p53/metabolismo , Adulto , Idoso , Biomarcadores Tumorais/metabolismo , Carcinoma de Células Escamosas/patologia , Carcinoma de Células Escamosas/cirurgia , Feminino , Glote/patologia , Glote/cirurgia , Humanos , Neoplasias Laríngeas/patologia , Neoplasias Laríngeas/cirurgia , Terapia a Laser , Masculino , Pessoa de Meia-Idade , Recidiva Local de Neoplasia/patologiaRESUMO
Mutations in the p53 gene--which codifies anuclear phosphoprotein that acts as a tumor suppressor gene--is the most common genetic alteration in head and neck cancers. The aim of the present study was to investigate the prognostic significance of p53 protein over expression in squamous cell laryngeal carcinoma. To do so we analyzed 31 patients affected by precancerous lesions of the larynx who had undergone multiple biopsy between 1980 and 1995. Twenty-five of these patients later developed laryngeal carcinoma. In this group of patients, 51 biopsies were performed for precancerous lesions (17 hyperplasia, 3 light dysplasia, 23 moderate dysplasia, 8 severe dysplasia) prior to evidence of laryngeal cancer (2.04 biopsies/patient). In the group of patients who did not develop laryngeal cancer, 18 biopsy were performed (2.2 biopsies/patient) and histology revealed: 5 keratosis, 5 light dysplasia, 4 moderate dysplasia and 4 grave dysplasia. Using the immunohistopathological staining technique, 69 formalin-fixed, paraffin-embedded precancerous samples and 25 laryngeal carcinomas were examined for p53 over expression. The monoclonal antibody Pab 1801 was used with the avidinbiotin immunoperoxidase technique; p53 intensity of expression was assessed and correlated with clinical-pathological parameters. Over expression of the p53 protein was found in 56.8% of the precancerous lesions (41% of the hyperplastic lesions, 66% of light dysplastic lesions, 60% of moderate dysplastic lesions and 75% of severe dysplastic lesions) in the group patients who did develop laryngeal cancer and in 22.2% of the precancerous lesions in the group of patients that did not. The transformed lesions showed a strong correlation between intensity of positivity and grade of cellular atypia. Further in 93.3% of the patients with p53 positive precancerous lesions which later developed into laryngeal cancer, p53 over expression was present in the cancerous lesions. There was no significant correlation between p53 immuno reactivity and such clinico pathological tumor parameters as TNM staging and tumorrecurrence. On the other hand, there was a correlation between p53 overexpression and differentiation grading: p53 overexpression was found in 75% of the poorly differentiated tumors, 58.3% of moderately differentiated and 44.4% of well differentiated tumors. The fact that p53 is detected in preneoplastic lesions suggests that p53 gene alteration takes place very early in laryngeal carcinoma and moderate-to-high p53 expression constitutes a high risk of transformation into cancer; on the other hand low expression may reflect reversible changes that can be attributed to the genotoxic effects of tobacco smoking. In conclusion the present data suggest that p53 over expression could be a good prognostic marker in predicting which precancerous laryngeal lesions will progress into cancer.
Assuntos
Carcinoma de Células Escamosas/genética , Carcinoma de Células Escamosas/patologia , Neoplasias de Cabeça e Pescoço/genética , Neoplasias de Cabeça e Pescoço/patologia , Proteína Supressora de Tumor p53/genética , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Seguimentos , Expressão Gênica , Humanos , Masculino , Pessoa de Meia-Idade , Lesões Pré-Cancerosas , Valor Preditivo dos Testes , Prognóstico , Estudos RetrospectivosRESUMO
The authors report a rare case of localized malignant pleural mesothelioma infiltrating the thoracic wall. The absence of widespread pleural involvement allowed for a radical operation with removal of a large portion of the thoracic wall. However, due to rarity of such tumor, an hypothesis on long term prognosis is difficult to formulate.
Assuntos
Mesotelioma/cirurgia , Neoplasias Pleurais/cirurgia , Humanos , Masculino , Mesotelioma/diagnóstico , Pessoa de Meia-Idade , Pleura/cirurgia , Neoplasias Pleurais/diagnóstico , ToracotomiaRESUMO
A case of primary leiomyosarcoma of the inter-renal vena cava is reported. This tumor is rare and up to date no more than 100 cases have been reported. Surgical treatment consisted in the removal of vena cava from the iliac veins to the suprahepatic veins with right nephrectomy. Restoration of vena cava and left renal vein continuity was not necessary because of the presence of collateral venous circulation at the time of operation. Venous ligation was haemodynamically well tolerated and the patient is disease-free at one year from operation. However, despite surgical radicality, prognosis remains poor given the frequency of metastases reported in more than 35% of cases.
Assuntos
Leiomiossarcoma/cirurgia , Neoplasias de Tecidos Moles/cirurgia , Veia Cava Inferior/cirurgia , Feminino , Humanos , Pessoa de Meia-IdadeRESUMO
The video-thoracoscopic treatment of spontaneous pneumothorax currently has the same role of laparoscopic cholecystectomy in abdominal surgery. The Authors consider thoracoscopic approach and traditional thoracotomy examining advantages versus disadvantages, comparing 50 patients with spontaneous pneumothorax treated by thoracoscopy, from February 1992 up to February 1995, and 50 patients, previously treated by open surgery. Video-thoracoscopy has the same percentage of recurrences of thoracotomic approach but assures a quicker functional recovery and, above all, a remarkable reduction of pain.
Assuntos
Pneumotórax/cirurgia , Toracoscopia , Toracotomia , Adulto , Feminino , Humanos , Masculino , Dor Pós-Operatória/prevenção & controle , Gravação em VídeoRESUMO
The Authors report a case of thyroid carcinoma with tracheal involvement. Since the neoplastic invasion of the trachea was 3 cm long total thyroidectomy with sleeve tracheal resection and immediate reconstruction with end-to-end anastomosis were performed. The diagnostic and therapeutic approach is discussed and the Literature data reviewed.
Assuntos
Carcinoma Papilar/cirurgia , Neoplasias da Glândula Tireoide/cirurgia , Traqueia/cirurgia , Neoplasias da Traqueia/cirurgia , Adulto , Anastomose Cirúrgica , Carcinoma Papilar/diagnóstico , Carcinoma Papilar/patologia , Feminino , Humanos , Invasividade Neoplásica , Glândula Tireoide/patologia , Neoplasias da Glândula Tireoide/diagnóstico , Neoplasias da Glândula Tireoide/patologia , Tireoidectomia , Tomografia Computadorizada por Raios X , Traqueia/patologiaRESUMO
From January '92 up to January '94 thoracoscopy was performed in 51 patients with recurrent pleural effusion: 49 of these patients underwent talc pleurodesis. 10 pleural mesotheliomas, 29 diffuse metastasic pleural involvement and 12 benign effusions were diagnosed. Intrapleural talc therapy allowed a definitive pleurodesis in 43 patients with a success rate of 87%.
Assuntos
Derrame Pleural/terapia , Pleurodese , Talco/administração & dosagem , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Recidiva , ToracoscopiaRESUMO
Lymphoma is the most common malignant orbital tumor. We describe the imaging features of diffuse orbital follicular lymphoma with extension into the pterygopalatine fossa and infratemporal fossa without bony infiltration.
RESUMO
Toluidine blue stain is used as a marker to differentiate lesions at high risk of progression in order to improve early diagnosis of oropharyngeal carcinomas. This study focused on 45 oral mucosal lesions in 32 patients (13 female, 19 male). In 9 cases, multiple biopsies were collected. Of the 45 lesions examined, 26 (57.0%) were defined clinically benign, while 19 (42.3%) were defined as suspected lesions (premalignant or malignant). According to the clinical examination, the sensitivity was 53% (16/30) and for toluidine blue staining 96.2% (26/27) (p = 0.0007). The specificity was 80% (12/15) for clinical examination and 77.7% (14/15) for toluidine blue staining (p = 0.79). In conclusion toluidine blue stain has been shown to be a reliable aid when clinical examination is unable to differentiate lesions at high risk of progression and then it improves early diagnosis for oral cavity and oropharyngeal cancer.
Assuntos
Carcinoma de Células Escamosas/patologia , Boca/patologia , Neoplasias Orofaríngeas/patologia , Lesões Pré-Cancerosas/patologia , Cloreto de Tolônio , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Valor Preditivo dos Testes , PrevalênciaRESUMO
Mature cystic teratoma is a benign neoplasm, but malignant transformation of one component may occur in 2% of cases. Although very different types of carcinomas may be arise from mature cystic teratoma, invasive squamous cell carcinoma is the most frequent type of malignancy found, comprising about 80% of all malignancies arising from dermoid tumours. Although invasive squamous cell carcinoma is relatively frequent, it is surprising that so few cases of squamous cell carcinoma in situ in mature cystic teratoma have been reported. We describe a case of squamous cell carcinoma in mature cystic teratoma without an invasive component.
Assuntos
Carcinoma de Células Escamosas/patologia , Neoplasias Primárias Múltiplas/patologia , Neoplasias Ovarianas/patologia , Teratoma/patologia , Feminino , Humanos , Pessoa de Meia-IdadeRESUMO
The thyroid-associated transcription factor-1 (TTF-1) is a nuclear tissue specific protein, found only in thyroid and thyroid tumors regardless of histologic type as well as in lung carcinomas. Most ectopic thyroid tissue are found along the thyroglossal duct tract and around the bilateral main lobes of thyroid glands. Ectopic thyroid tissue within a branchial cleft cyst is a rare abnormality and papillary carcinoma arising in this tissue is extremely rare. Thyroid tissue may also be found as one of the many components of teratoma, particularly those located in the ovary. The aim of this study is to evaluate, through immunohistochemistry, the expression of TTF-1 in branchial cleft cysts with ectopic normal thyroid tissue, in primary thyroid papillary carcinomas of branchial cleft cysts and thyroglossal duct cysts and in struma ovarii, in order to evaluate the expression of this antigen in extra thyroidal sites.
Assuntos
Branquioma/metabolismo , Carcinoma Papilar/metabolismo , Neoplasias de Cabeça e Pescoço/metabolismo , Proteínas Nucleares/biossíntese , Neoplasias Ovarianas/metabolismo , Estruma Ovariano/metabolismo , Cisto Tireoglosso/metabolismo , Neoplasias da Glândula Tireoide/metabolismo , Fatores de Transcrição/biossíntese , Adolescente , Adulto , Criança , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Fator Nuclear 1 de TireoideRESUMO
We have reported two cases of cerebral gliosarcoma by immunohistochemical method. The antibodies anti S-100, anti GFAP and anti vimentin have supported the presence of glial and mesenchymal elements. Factor VIII has labeled the endothelium of the newly formed vessels. Collagen IV has shown atypical cells in the thickness of the vascular walls, and ribbons of adventitial cells throwing in the surrounding parenchyma. By these results, the pericitic origin of the sarcomatous elements could be hypothesized.
Assuntos
Neoplasias Encefálicas/patologia , Glioma/patologia , Lobo Parietal , Sarcoma/patologia , Lobo Temporal , Humanos , Masculino , Pessoa de Meia-IdadeRESUMO
We have chosen 10 medullary carcinomas from a retrospective investigation on 450 cases of breast carcinoma (2.2%), according to the histomorphological criteria of Ridolfi and colleagues. By immunoperoxidase staining, we have evaluated the distribution of keratin and vimentin intermediate filaments, of CEA and S-100 protein in cancer cells. A widespread positivity to keratins has been proved in 70% of medullary carcinomas. Numerous neoplastic cells have coexpressed S-100 protein. We hypothesize the epithelial and myoepithelial "intermediate" differentiation of keratin and S-100 protein positive tumour cells.