Plant nitrogen availability and crosstalk with phytohormones signallings and their biotechnology breeding application in crops.

Nitrogen (N), one of the most important nutrients, limits plant growth and crop yields in sustainable agriculture system, in which phytohormones are known to play essential roles in N availability. Hence, it is not surprising that massive studies about the crosstalk between N and phytohormones have been constantly emerging. In this review, with the intellectual landscape of N and phytohormones crosstalk provided by the bibliometric analysis, we trace the research story of best-known crosstalk between N and various phytohormones over the last 20 years. Then, we discuss how N regulates various phytohormones biosynthesis and transport in plants. In reverse, we also summarize how phytohormones signallings modulate root system architecture (RSA) in response to N availability. Besides, we expand to outline how phytohormones signallings regulate uptake, transport, and assimilation of N in plants. Further, we conclude advanced biotechnology strategies, explain their application, and provide potential phytohormones-regulated N use efficiency (NUE) targets in crops. Collectively, this review provides not only a better understanding on the recent progress of crosstalk between N and phytohormones, but also targeted strategies for improvement of NUE to increase crop yields in future biotechnology breeding of crops.

High Heterotrophic Plasticity of Massive Coral Porites pukoensis Contributes to Its Tolerance to Bioaccumulated Microplastics.

Scleractinian corals have been observed to be capable of accumulating microplastics from reef environments; however, the tolerant mechanism is poorly known. Here, we examined the response of Porites pukoensis to microplastic pollution by analyzing algal symbiont density, energetic metabolism, and caspase3 activities (representing the apoptosis level) in the coral-Symbiodiniaceae association. The environments of three fringing reef regions along the south coast of Sanya City, Hainan Province of China, were polluted by microplastics (for example, microplastic concentrations in the seawater ranged from 3.3 to 46.6 particles L-1), resulting in microplastic accumulation in P. pukoensis (0.4-2.4 particles cm-2). The accumulation of microplastics was negatively correlated to algal symbiont density in the corals but not to caspase3 activities in the two symbiotic partners, demonstrating that P. pukoensis could tolerate accumulated microplastics despite the decrease of algal symbiont density. Furthermore, results from the carbon stable isotope and cellular energy allocation assay indicated that P. pukoensis obtained energy availability (mainly as lipid reserves) using the switch between heterotrophy and autotrophy to maintain energy balance and cope with accumulated microplastics. Collectively, P. pukoensis achieved tolerance to microplastic pollution by maintaining energy availability, which was largely attributed to its high heterotrophic plasticity.

Characterization, reactivity, source apportionment, and potential source areas of ambient volatile organic compounds in a typical tropical city.

Based on one-year observation, the concentration, sources, and potential source areas of volatile organic compounds (VOCs) were comprehensively analyzed to investigate the pollution characteristics of ambient VOCs in Haikou, China. The results showed that the annual average concentration of total VOCs (TVOCs) was 11.4 ppbV, and the composition was dominated by alkanes (8.2 ppbV, 71.4%) and alkenes (1.3 ppbV, 20.5%). The diurnal variation in the concentration of dominant VOC species showed a distinct bimodal distribution with peaks in the morning and evening. The greatest contribution to ozone formation potential (OFP) was made by alkenes (51.6%), followed by alkanes (27.2%). The concentrations of VOCs and nitrogen dioxide (NO2) in spring and summer were low, and it was difficult to generate high ozone (O3) concentrations through photochemical reactions. The significant increase in O3 concentrations in autumn and winter was mainly related to the transmission of pollutants from the northeast. Traffic sources (40.1%), industrial sources (19.4%), combustion sources (18.6%), solvent usage sources (15.5%) and plant sources (6.4%) were identified as major sources of VOCs through the positive matrix factorization (PMF) model. The southeastern coastal areas of China were identified as major potential source areas of VOCs through the potential source contribution function (PSCF) and concentration-weighted trajectory (CWT) models. Overall, the concentration of ambient VOCs in Haikou was strongly influenced by traffic sources and long-distance transport, and the control of VOCs emitted from vehicles should be strengthened to reduce the active species of ambient VOCs in Haikou, thereby reducing the generation of O3.

Mechanical regulation of the helicase activity of Zika virus NS3.

Zika virus (ZIKV) is a positive-sense single-stranded RNA virus that infects humans and can cause birth defects and neurological disorders. Its non-structural protein 3 (NS3) contains a protease domain and a helicase domain, both of which play essential roles during the viral life cycle. However, it has been shown that ZIKV NS3 has an inherently weak helicase activity, making it unable to unwind long RNA duplexes alone. How this activity is stimulated to process the viral genome and whether the two domains of NS3 are functionally coupled remain unclear. Here, we used optical tweezers to characterize the RNA-unwinding properties of ZIKV NS3-including its processivity, velocity, and step size-at the single-molecule level. We found that external forces that weaken the stability of the duplex RNA substrate significantly enhance the helicase activity of ZIKV NS3. On the other hand, we showed that the protease domain increases the binding affinity of NS3 to RNA but has only a minor effect on unwinding per se. Our findings suggest that the ZIKV NS3 helicase is activated on demand in the context of viral replication, a paradigm that may be generalizable to other flaviviruses.

GhTULP34, a member of tubby-like proteins, interacts with GhSKP1A to negatively regulate plant osmotic stress.

Tubby-like protein genes (TULPs), present in the form of large multigene families, play important roles in environmental stress. However, little is known regarding the TULP family genes in cotton. In this study, we systematically identified and analyzed the membership, characterization, and evolutionary relationship of TULPs in four species of cotton. Transcriptome analysis indicated that GhTULPs participate in environmental stress and cotton tissue development. At the same time, we also predicted and analyzed the potential molecular regulatory mechanisms and functions of TULPs. GhTULP34, as a candidate gene, significantly reduced the germination rate of transgenic Arabidopsis plants under salt stress, and inhibited root development and stomatal closure under mannitol stress. The yeast two-hybrid and luciferase (LUC) systems showed that GhTULP34 can interact with GhSKP1A, a subunit of the SCF-type (Skp1-Cullin-1-F-box) complex. This study will provide a basis and reference for future research on their roles in stress tolerance.

Force-dependent stimulation of RNA unwinding by SARS-CoV-2 nsp13 helicase.

The superfamily 1 helicase nonstructural protein 13 (nsp13) is required for SARS-CoV-2 replication. The mechanism and regulation of nsp13 has not been explored at the single-molecule level. Specifically, force-dependent unwinding experiments have yet to be performed for any coronavirus helicase. Here, using optical tweezers, we find that nsp13 unwinding frequency, processivity, and velocity increase substantially when a destabilizing force is applied to the RNA substrate. These results, along with bulk assays, depict nsp13 as an intrinsically weak helicase that can be activated >50-fold by piconewton forces. Such force-dependent behavior contrasts the known behavior of other viral monomeric helicases, such as hepatitis C virus NS3, and instead draws stronger parallels to ring-shaped helicases. Our findings suggest that mechanoregulation, which may be provided by a directly bound RNA-dependent RNA polymerase, enables on-demand helicase activity on the relevant polynucleotide substrate during viral replication.

Knockdown of AMP-activated protein kinase increases the insecticidal efficiency of pymetrozine to Nilaparvata lugens.

Insecticides are the main tools used to control Nilaparvata lugens (Stål), a serious pest of rice in Asia. However, repeated application of insecticides has caused many negative effects. Reducing the amount of insecticide used, while maintaining good pest population control, would be valuable. AMP-activated protein kinase (AMPK), a sensor of cellular energy status, helps to maintain insect energy balance at the cellular and whole-body level. The role of AMPK in insect response to insecticide stimulation is unknown. We studied the functions of AMPK catalytic subunit alpha (NlAMPKα) in the development of N. lugens and in response to pymetrozine, an insecticide used to control insect pests with piercing-sucking mouthparts. A phylogenetic analysis of protein sequences from 12 species in six orders showed that insects have only the AMPKα 2 subtype. RNA interference against NlAMPKα demonstrated that blocking the AMPK pathway led to a decrease in the systemic ATP level and an increase in N. lugens mortality. NlAMPKα responded to the energy stress caused by pymetrozine treatment, which activated downstream energy metabolic pathways to compensate for the energy imbalance. However, the ATP level in pymetrozine- treated nymphs was not increased, suggesting that ATP is consumed more than synthesized. When NlAMPKα expression was reduced in pymetrozine-treated nymphs by RNAi, the ATP level was decreased and the mortality was significantly increased. At day eight post 0.5 g/3 L of pymetrozine and dsNlAMPKα treatment, nymph survival was 29.33%, which was similar to the 27.33% survival of 1 g/3 L pymetrozine-treated nymphs. Addition of dsNlAMPKα can reduce the concentration of pymetrozine used by 50% while providing comparable efficacy. These results indicate that AMPK helps maintain the energy metabolism of N. lugens in response to pymetrozine treatment. Knockdown of NlAMPKα increases the insecticidal efficiency of pymetrozine to N. lugens.

Molecular mechanism of divalent-metal-induced activation of NS3 helicase and insights into Zika virus inhibitor design.

Zika virus has attracted increasing attention because of its potential for causing human neural disorders, including microcephaly in infants and Guillain-Barré syndrome. Its NS3 helicase domain plays critical roles in NTP-dependent RNA unwinding and translocation during viral replication. Our structural analysis revealed a pre-activation state of NS3 helicase in complex with GTPγS, in which the triphosphate adopts a compact conformation in the absence of any divalent metal ions. In contrast, in the presence of a divalent cation, GTPγS adopts an extended conformation, and the Walker A motif undergoes substantial conformational changes. Both features contribute to more extensive interactions between the GTPγS and the enzyme. Thus, this study provides structural evidence on the allosteric modulation of MgNTP2- on the NS3 helicase activity. Furthermore, the compact conformation of inhibitory NTP identified in this study provides precise information for the rational drug design of small molecule inhibitors for the treatment of ZIKV infection.

Selective enrichment of bacteria and antibiotic resistance genes in microplastic biofilms and their potential hazards in coral reef ecosystems.

Microplastics become hotspots for bacteria to trigger a series of ecological effects, but few studies have focused on the potential impacts of microplastic biofilms in coral reef ecosystems. Here, we measured the bacterial communities and antibiotic resistance genes (ARGs) in the seawater and microplastic biofilms. Results showed that microbial biofilms were formed on the surface of microplastics. The alpha diversity of the bacterial community in the microplastic biofilms was lower than that in the seawater, and the bacterial communities were distinct between the two. Further analysis revealed that several bacteria in the microplastic biofilms carried ARGs, and the proportion of which was correlated to the concentration of antibiotics in the seawater. Specifically, Vibrio was positively correlated to sul1 in the microplastic biofilms under higher concentrations of sulfonamides. Pathway analysis reflected significant overrepresentation of human disease related pathways in the bacterial community of microplastic biofilms. These results suggest that the microplastic biofilms could selectively enrich bacteria from the reef environments, causing the development of ARGs under antibiotic driving. This may pose a serious threat to coral reef ecosystems and human health. Our study provides new insights into the ecological impacts of microplastic biofilms in coral reef ecosystems.

Differential bioaccumulation and tolerances of massive and branching scleractinian corals to polycyclic aromatic hydrocarbons in situ.

Scleractinian corals are capable of accumulating polycyclic aromatic hydrocarbons (PAHs) in reef environments; however, the mechanism behind their PAHs tolerance is unknown. This study investigated the occurrence and bioaccumulation of PAHs in coral reef ecosystems and examined the physiological responses induced by PAHs in coral hosts and their algal symbionts, the massive coral Galaxea fascicularis and branching coral Pocillopora damicornis. G. fascicularis had a higher PAHs accumulation capacity than P. damicornis. Both the coral hosts and algal symbionts preferentially accumulated acenaphthene, dibenzo(a,h)anthracene, and benzo(a)pyrene. The accumulated PAHs by G. fascicularis and P. damicornis hosts was accompanied by a reduction in detoxification ability. The accumulated PAHs could induce oxidative stress in P. damicorni hosts, thus G. fascicularis demonstrated a greater tolerance to PAHs compared to P. damicornis. Meanwhile, their algal symbionts had fewer physiological responses to accumulated PAHs than the coral hosts. Negative effects were not observed with benzo(a)pyrene. Taken together, these results suggest massive and branching scleractinian corals have different PAHs bioaccumulation and tolerance mechanisms, and indicate that long-term PAHs pollution could cause significant alterations of community structures in coral reef ecosystems.

Occurrence, distribution, and risk assessment of antibiotics in a typical aquaculture area around the Dongzhai Harbor mangrove forest on Hainan Island.

The trees of the Dongzhai Harbor mangrove forest suffer from antibiotic contamination from surrounding aquaculture areas. Despite this being one of the largest mangrove forests in China, few studies have focused on the antibiotic pollution status in these aquaculture areas. In the present study, the occurrence, distribution, and risk assessment of 37 antibiotics in surface water and sediment samples from aquaculture areas around Dongzhai Harbor mangrove forests were analyzed. The concentration of total antibiotics (∑antibiotics) ranged from 78.4 ng/L to 225.6 ng/L in surface water (except S14-A2) and from 19.5 ng/g dry weight (dw) to 229 ng/g dw in sediment. In the sediment, the concentrations of ∑antibiotics were relatively low (19.5-52.3 ng/g dw) at 75 % of the sampling sites, while they were high (95.7-229.0 ng/g dw) at a few sampling sites (S13-A1, S13D, S8D). The correlation analysis results showed that the Kd values of the 9 antibiotics were significantly positively correlated with molecular weight (MW), Kow, and LogKow. Risk assessment revealed that sulfamethoxazole (SMX) in surface water and SMX, enoxacin (ENX), ciprofloxacin (CFX), enrofloxacin (EFX), ofloxacin (OFX), and norfloxacin (NFX) in sediment had medium/high risk quotients (RQs) at 62.5 % and 25-100 %, respectively, of the sampling sites. The antibiotic mixture in surface water (0.06-3.36) and sediment (0.43-309) posed a high risk at 37.5 % and 66.7 %, respectively, of the sampling sites. SMX was selected as an indicator of antibiotic pollution in surface water to assist regulatory authorities in monitoring and managing antibiotic pollution in the aquaculture zone of Dongzhai Harbor. Overall, the results of the present study provide a comprehensive and detailed analysis of the characteristics of antibiotics in the aquaculture environment around the Dongzhai Harbor mangrove system and provide a theoretical basis for the source control of antibiotics in mangrove systems.

Updating vehicle VOCs emissions characteristics under clean air actions in a tropical city of China.

In the context of clean air actions in China, vehicle emission limits have been continuously tightened, which has facilitated the reduction of volatile organic compounds (VOCs) emissions. However, the characteristics of VOC emissions from vehicles with strict emission limits are poorly understood. This study investigated the VOC emission characteristics from vehicles under the latest standards based on tunnel measurements, and identified future control strategies for vehicle emissions. The results showed that the highest percentage of VOCs from vehicle consisted of alkanes (80.9 %), followed by aromatics (15.8 %) and alkenes (3.1 %). Alkanes had the most significant ozone formation potential due to their high concentrations, in contrast to the aromatics that have been dominant in previous studies. The measured fleet-average VOC emission factor was 71.3 mg·km-1, including tailpipe emissions of 39.6 mg·km-1 and evaporative emissions of 31.7 mg·km-1. The VOC emission factors of the subgroups were obtained. The emission of evaporated VOCs accounted for 44.5 % of the total vehicle VOC emissions, which have increased substantially from previous studies. In addition, the emission characteristics of vehicles that are under the latest emission threshold values have changed significantly, and the mixing ratio of toluene/benzene (T/B) has been updated to 3:1. This study updates the VOCs emission factors of vehicles under clean air actions and highlights the future mitigation policies should focus on reducing evaporative VOC emissions.

Unraveling the metabolic effects of benzophenone-3 on the endosymbiotic dinoflagellate Cladocopium goreaui.

As a well-known pseudo-persistent environmental pollutant, oxybenzone (BP-3) and its related organic ultraviolet (UV) filters have been verified to directly contribute to the increasing mortality rate of coral reefs. Previous studies have revealed the potential role of symbiotic Symbiodiniaceae in protecting corals from the toxic effects of UV filters. However, the detailed protection mechanism(s) have not been explained. Here, the impacts of BP-3 on the symbiotic Symbiodiniaceae Cladocopium goreaui were explored. C. goreaui cells exhibited distinct cell growth at different BP-3 doses, with increasing growth at the lower concentration (2 mg L-1) and rapid death at a higher concentration (20 mg L-1). Furthermore, C. goreaui cells showed a significant BP-3 uptake at the lower BP-3 concentration. BP-3 absorbing cells exhibited elevated photosynthetic efficiency, and decreased cellular carbon and nitrogen contents. Besides, the derivatives of BP-3 and aromatic amino acid metabolism highly responded to BP-3 absorption and biodegradation. Our physiological and metabolic results reveal that the symbiotic Symbiodiniaceae could resist the toxicity of a range of BP-3 through promoting cell division, photosynthesis, and reprogramming amino acid metabolism. This study provides novel insights into the influences of organic UV filters to coral reef ecosystems, which urgently needs increasing attention and management.

Force-dependent stimulation of RNA unwinding by SARS-CoV-2 nsp13 helicase.

The superfamily-1 helicase non-structural protein 13 (nsp13) is required for SARS-CoV-2 replication, making it an important antiviral therapeutic target. The mechanism and regulation of nsp13 has not been explored at the single-molecule level. Specifically, force-dependent unwinding experiments have yet to be performed for any coronavirus helicase. Here, using optical tweezers, we find that nsp13 unwinding frequency, processivity, and velocity increase substantially when a destabilizing force is applied to the dsRNA, suggesting a passive unwinding mechanism. These results, along with bulk assays, depict nsp13 as an intrinsically weak helicase that can be potently activated by picoNewton forces. Such force-dependent behavior contrasts the known behavior of other viral monomeric helicases, drawing stronger parallels to ring-shaped helicases. Our findings suggest that mechanoregulation, which may be provided by a directly bound RNA-dependent RNA polymerase, enables on-demand helicase activity on the relevant polynucleotide substrate during viral replication.

An accelerated solvent extraction and gas chromatography-flame ionization detector method to rapidly determining and assessing total petroleum hydrocarbon contamination in soil from Fushan oilfield, China.

A high-efficient method for determining the total petroleum hydrocarbon (TPH) was established by gas chromatography-flame ionization detection, coupled with an efficient 10 m short chromatographic column; the analyzing period was narrowed to 5 mins. The limits of detection of the method included 1.47, 4.02, and 0.69 mg/kg, and the corresponding limits of quantification reached 4.45, 12.2, and 2.10 mg/kg for the three fractions C10-C16, C17-C34, and C35-C40, respectively. The method was employed to real samples to achieve the routine environmental monitoring of TPH in polluted sites from Fushan oilfield, China. As revealed from the analysis of 30 soil samples in the study area, a wide range of TPH concentrations were achieved: 61.6-7300 mg/kg (average, 1055 mg/kg) for ΣC10-C16, 438-14,280 mg/kg (average, 4544 mg/kg) for ΣC17-C34, 25.4-638 mg/kg (average, 250 mg/kg) for ΣC35-C40, and 617-15,348 (average, 5848 mg/kg) for ΣC10-C40, respectively. According to the Nemerow integrated pollution index, the Fushan oilfield has been slightly polluted by TPH. As suggested from the distribution of TPH concentrations, the main sources of TPH in soil samples of Fushan oilfield included oil spills during temporary storage, transportation, and oil exploitation. Adopting the developed method to delve into oilfield soil samples further verifies the effectiveness of the method, indicating that the method can well meet the growing demand of regulatory guidelines for related risk assessment and environmental monitoring and remediation strategy formulation.

Graphene quantum dots: efficient mechanosynthesis, white-light and broad linear excitation-dependent photoluminescence and growth inhibition of bladder cancer cells.

Heteroatom-doped graphene quantum dots (GQDs) have attracted considerable attention due to their potential applications as luminescent materials and in biology. In this work, we developed a solvent-free gram-scale mechanochemical method for the preparation of nitrogen-doped graphene quantum dots (N-GQDs) with the highest solubility (31 mg mL-1) in water reported to date. Commercial graphite was sheared and cut through grinding with solid melamine and then ground with solid KOH to get sub-5 nm-sized, 1-3-layered N-GQDs. Notably, these N-GQDs exhibit white-light emission and broad excitation-dependent full-color photoluminescence from 463 nm to 672 nm. When the excitation light ranged from 325 nm to 485 nm, these mechanochemically obtained N-GQDs exhibited bright white-light emission. Intriguingly, the change in the emission wavelength has two-stage linear relationships with the change in the excitation wavelength, and the inflection point is at 580 nm (excited at 550 nm). The difference between the emission and excitation wavelengths decreases from 138 to 12 nm, which also shows two-stage linear relationships with the change in the excitation wavelength. It is notable that their PL quantum yields are high, up to 26.6%. Furthermore, we studied the inhibitory effect of as-obtained N-GQDs on bladder cancer cells (UMUC-3); as a result, with the increase of the concentration of N-GQDs, the proliferation of cancer cells was obviously prohibited.

Crystal structure of caspase-11 CARD provides insights into caspase-11 activation.

Murine caspase-11 is the centerpiece of the non-canonical inflammasome pathway that can respond to intracellular LPS and induce pyroptosis. Caspase-11 contains two components, an N-terminal caspase recruitment domain (CARD) and a C-terminal catalytic domain. The aggregation of caspase-11 is thought to promote the auto-processing and activation of caspase-11. However, the activation mechanism of caspase-11 remains unclear. In this study, we purified the caspase-11 CARD fused to an MBP tag and found it tetramerizes in solution. Crystallographic analysis reveals an extensive hydrophobic interface formed by the H1-2 helix mediating homotypic CARD interactions. Importantly, mutations of the helix H1-2 hydrophobic residues abolished the tetramerization of MBP-tagged CARD in solution and failed to induce pyroptosis in cells. Our study provides the first evidence of the homotypic interaction mode for an inflammatory caspase by crystal model. This finding demonstrates that the tetramerization of the N-terminal CARD can promote releasing of the catalytic domain auto-inhibition, leading to the caspase-11 activation.

[Rapid determination of glyphosate, aminomethyl phosphonic acid, glufosinate, and ethephon residues in environmental water by direct injection-ultra performance liquid chromatography-triple quadrupole mass spectrometry].

A simple method based on direct injection-ultra performance liquid chromatography-triple quadrupole tandem mass spectrometry (UPLC-MS/MS) was established for the rapid determination of glyphosate, aminomethyl phosphonic acid, glufosinate, and ethephon residues in environmental water. The water samples were filtered through a 0.22-µm filter membrane or frozen and centrifuged to remove impurities, and then, the filtrate was directly subjected to quantitative analysis without derivatization. The analytes were separated on a Metrosep A Supp 5 column (150 mm×4.0 mm, 5 µm), and gradient elution was carried out using an ammonium bicarbonate-ammonia solution as the mobile phase. The data were collected by positive electrospray ionization in the multiple reaction monitoring (MRM) mode. The results showed that the correlation coefficients (r) of the linear calibration curves were greater than 0.999 in the corresponding linear ranges (0.50-50.0 µg/L). The detection limits of the analytes were 0.05-0.09 µg/L. The recoveries of glyphosate, aminomethyl phosphonic acid, glufosinate, and ethephon were in the ranges 76.3%-108%, 83.0%-107%, and 87.0%-105% at low, medium, and high spiked levels, respectively. The corresponding relative standard deviations were in the ranges 2.0%-12.3%, 2.4%-5.6%, and 2.7%-6.8%. Using this method, 34 water samples collected from Hainan Province were analyzed, among which 30 drinking water sources were found to be free from the four pesticides. Glyphosate and aminomethyl phosphonic acid were detected in three water samples near a betel nut orchard, while glufosinate and aminomethyl phosphonic acid were detected in a water sample near a banana orchard. This method is advantageous over the traditional derivatization method because of its simple operation, good reproducibility, and high accuracy; furthermore, the matrix interference effect is absent. Thus, this method is suitable for analyzing glyphosate, aminomethyl phosphonic acid, glufosinate, and ethephon residues in environmental water samples.

Functional and structural characterization of zebrafish ASC.

The zebrafish genome encodes homologs for most of the proteins involved in inflammatory pathways; however, the molecular components and activation mechanisms of fish inflammasomes are largely unknown. ASC [apoptosis-associated speck-like protein containing a caspase-recruitment domain (CARD)] is the only adaptor involved in the formation of multiple types of inflammasomes. Here, we demonstrate that zASC is also involved in inflammasome activation in zebrafish. When overexpressed in vitro and in vivo in zebrafish, both the zASC and zASC pyrin domain (PYD) proteins form speck and filament structures. Importantly, the crystal structures of the N-terminal PYD and C-terminal CARD of zebrafish ASC were determined independently as two separate entities fused to maltose-binding protein. Structure-guided mutagenesis revealed the functional relevance of the PYD hydrophilic surface found in the crystal lattice. Finally, the fish caspase-1 homolog Caspy, but not the caspase-4/11 homolog Caspy2, interacts with zASC through homotypic PYD-PYD interactions, which differ from those in mammals. These observations establish the conserved and unique structural/functional features of the zASC-dependent inflammasome pathway. DATABASE: Structural data are available in the PDB under accession numbers 5GPP and 5GPQ.

Pattern recognition receptors in zebrafish provide functional and evolutionary insight into innate immune signaling pathways.

Pattern recognition receptors (PRRs) and their signaling pathways have essential roles in recognizing various components of pathogens as well as damaged cells and triggering inflammatory responses that eliminate invading microorganisms and damaged cells. The zebrafish relies heavily on these primary defense mechanisms against pathogens. Here, we review the major PRR signaling pathways in the zebrafish innate immune system and compare these signaling pathways in zebrafish and humans to reveal their evolutionary relationship and better understand their innate immune defense mechanisms.