[Antiproliferative activity of diclofenac at tumor cell cultures].

It is known that for risk group's importance of early diagnostics and prevention of a cancer of a thick section of the intestines. The view was expressed that the use of nonsteroidal anti-inflammatory drugs prospectively. The article presents the data obtained after the determination of the effect on the domestic drug diclofenac on the ability of the lines of tumor cells of the human colon multiply this antiproliferativoe action on the dose of the drug. Used photometric method for determination antiproliferativoe action. In vitro determined the effect of the national drug diclofenac on the ability of the lines of tumor cells of the human colon multiply, and is set in a dose-dependent effect. It is established that under the action of NSAIDS metabolic activity of cells can both strengthen and weaken that depends on the line of tumor cells and the concentration of the drug.

The functional state of thymus cells following microwave exposure of endocrine glands.

Following local microwave exposure (460 MHz, 120 mW/cm2) of rabbits using fluorescent probes and chemiluminescent analysis, conformational rearrangements in nuclear and cytoplasmic membranes and in thymus cell chromatin of the animals as well as changes in the intensity of the lipid free radical peroxidation were seen. The character of the changes observed depended both on the localization of the exposure and on the number of exposures.

[State of cerebral neuronal nuclear chromatin during multimodal stimuli to the body]. / Sostoianie iadernogo khromatina neironov golovnogo mozga pri raznomodal'nykh vozdeistviiakh na organizm.

The state of nuclear DNA, revealed by the microfluorometric method, changed unequally in conditions of dissimilar afferentation in different classes of the neurones in the neocortex and the hippocampal dentate fascia. It may be assumed that the examined classes of cells (large pyramids and stellate neurones of the sensorimotor zone of the cerebral cortex and the granular cells of the dentate fascia of the hippocampus) possess a different structural functional organization of genome. This probably accounts for the peculiarities of their functioning.

[The immunomodulating action of microwaves in the induction of an immune response to Vi antigen]. / Immunomoduliruiushchee deistvie mikrovoln pri induktsii immunnogo otveta na Vi-antigen.

In this work the possibility of using microwaves for immunomodulation in the immunization of animals with thymus-independent antigen was studied. The projection zones of the thymus and adrenal glands of the test animals were subjected to the action of decimeter, or ultrahigh frequency (UHF), waves, while the corresponding zones of the control animals were subjected to imitation UHF irradiation. Vi-antigen was shown to be a thymus-independent antigen for rabbits (according to the results of the evaluation of the functional state of thymocytes, as manifested by the adsorption of acridine orange by the chromatin of thymocyte nuclei and by the content of RNA and DNA in the thymus). The action of UHF waves on the projection zone of the thymus was accompanied by a decrease in the glucocorticoid activity of the adrenal cortex, observed simultaneously with a pronounced immunostimulating effect. The UHF irradiation of the zone of the adrenal glands was accompanied by immunosuppression in combination with enhanced glucocorticoid activity of the adrenal cortex.

[State of the mitochondria and nuclear chromatin in cerebral cortex nerve cells during laser irradiation]. / Sostoianie mitokhondrii khromatina iader nervnykh kletok kory golovnogo mozga pri lazernom obluchenii.

Effect of laser irradiation (PPM-mv/cm2, gamma = 632.8 nm) on respiratory and phosphorylating activities of mitochondria as well as on structure-functional state of nuclear chromatin from rat brain cortex neurones were studied. The irradiation stimulated the activities studied suggesting possible increase in functional potentialities of brain tissue under these experimental conditions.

[Nature of physico-chemical changes in the membranes and chromatin of the lymphocyte nuclei in patients with rheumatoid arthritis]. / Kharakter fiziko-khimicheskikh izmenenii membran i khromatina iader limfotsitov u bol'nykh revmatoidnym artritom.

Functional activity of lymphocytes, revealed by means of fluorometric study of the physicochemical properties of the membranes (according to the degree of binding with MBD) and nuclei (according to the degree of AO adsorption on chromatin DNA) was different in patients suffering from rheumatoid arthritis with different blood titers of RF determined by Vaaler-Rose's test. The lowered lymphocyte count in blood plasma was coupled with high functional activity of genome in patients with seronegative RA and with low in seropositive RA patients. Changes in the fluorescence of MBD bound with the membrane differed from those in the fluorescence of AO bound with lymphocyte chromatin. The DNA level in blood plasma of patients with seropositive RA was higher as compared with its levels seen in seronegative patients and healthy subjects.