Tailor-designed carbon-based novel fluorescent architecture for nanomolar detection of radioactive elements U(VI) and Th(IV) in pH ± 5.0.

Highly stable nitrogen-doped Graphene Quantum Dots (N-GQD) functionalized with Pamoic Acid (PA@N-GQD) are utilized for nanomolar detection of radioactive elements, Uranium (VI) and Thorium (IV), in pH ± 5.0. The absorption, fluorescence, crystalline nature, elemental composition, functional groups, and morphological state of as-prepared PA@N-GQD are evaluated by UV-visible absorption, photoluminescence, XRD, XPS, FTIR, HRTEM, FESEM, and AFM characterizations. The aqueous solution of PA@N-GQD is characterized by its spherical morphology, averaging 6.5 nm in size. PA@N-GQD exhibits a gradual decrease in fluorescence intensity at 438 nm (λex 344 nm) upon the addition of Uranium (VI) and Thorium (IV) ions. The selectivity, sensitivity, competitivity, pH, time effect, and reversibility studies of PA@N-GQDs have been carried out using the photoluminescence technique. The attained fluorescence Limit of Detection (LoD) of PA@N-GQD for Uranium (VI) and Thorium (IV) ions are 2.009 × 10-9 and 1.351 × 10-9 M, respectively. From the fluorescence titration studies of U(VI) and Th(IV), the binding constant, Stern-Volmer constant, Modified Stern-Volmer constant, association constant, and dissociation constants have been calculated separately. These aforementioned results indicate that the PA@N-GQD has a higher binding affinity towards Th(IV) than U(VI) in aqueous medium. This current research represents the development of advanced materials for environmental and analytical applications, specifically focusing on the precise detection and quantification of radioactive elements.

Waste derived approach towards wealthy fluorescent N-doped graphene quantum dots for cell imaging and H2O2 sensing applications.

Herein, we report the synthesis of a highly fluorescent nitrogen doped graphene quantum dots (N-GQDs) from waste precursors such as melamine sponge and arjuna bark via a microwave treatment and its functional and morphological characterization using various spectroscopy techniques such as optical, FTIR, XPS and TEM. The as-prepared aqueous N-GQD (dia. 2-3 nm) was used for the bio-imaging application using breast carcinoma cell line (MDA-MB-231) as a model, and the locations of all cells in the cytoplasm as well as nuclei were observed to stain brightly in blue fluorescent color successfully. In addition to that, the aqueous N-GQD showed fluorescence quenching behavior in the presence of hydrogen peroxide, which was exploited to sense H2O2, a probable toxin generated in the diseased cells. Importantly, the cell cytotoxicity was measured and found to be non-toxic (70% survival) to the MDA-MB-231 cells even at very high concentration (∼1.8 mg/ml) of the synthesized N-GQD. This study revealing excellent biocompatibility and imaging of the model cancer cells, and sensing of H2O2 by fluorescent quenching, indicates potential in-vivo cell culture applications of the prepared fluorescent N-GQD.

Development of an Efficient Immunosensing Platform by Exploring Single-Walled Carbon Nanohorns (SWCNHs) and Nitrogen Doped Graphene Quantum Dot (N-GQD) Nanocomposite for Early Detection of Cancer Biomarker.

In this work, a novel electrochemical immunosensor based on nitrogen doped graphene quantum dot (N-GQD) and single-walled carbon nanohorns (SWCNHs) was developed for the detection of α-fetoprotein (AFP), a cancer biomarker. Thus, to fabricate the platform of the immunosensor, nanocomposite architecture was developed by decorating N-GQD on the surface of the SWCNHs. The resulting hybrid architecture (N-GQD@SWCNHs) functioned as an exceptional base for the immobilization of antibody (Anti-AFP) through carbodiimide reaction with good stability and bioactivity. The immunosensor was prepared by evenly distributing the bioconjugates (N-GQD@SWCNHs/Anti-AFP) dispersion on the surface of the glassy carbon electrode, and subsequently blocking the remaining active sites by bovine serum albumin to prevent the nonspecific adsorption. Cyclic voltammetry and electrochemical impedance spectroscopy technique was employed to investigate the assembly process of the immunosensor. Under optimal conditions, the immunosensor exhibited a broad dynamic range in between 0.001 ng/mL to 200 ng/mL and a low detection limit of 0.25 pg/mL. Furthermore, the sensor showed high selectivity, desirable stability, and reproducibility. Measurements of AFP in human serum gave outstanding recovery within 99.2% and 102.1%. Thus, this investigation and the amplification strategy exhibited a potential role of the developed nanocomposite based sensor for early clinical screening of cancer biomarkers.