Your browser doesn't support javascript.
loading
تبين: 20 | 50 | 100
النتائج 1 - 4 de 4
المحددات
إضافة المرشحات








نوع الدراسة
النطاق السنوي
1.
Mem. Inst. Oswaldo Cruz ; 111(9): 570-576, Sept. 2016. tab, graf
مقالة ي الانجليزية | LILACS | ID: lil-794731

الملخص

Abstract Plasmodium falciparum and Plasmodium vivax have evolved with host switches between non-human primates (NHPs) and humans. Studies on the infection dynamics of Plasmodium species in NHPs will improve our understanding of the evolution of these parasites; however, such studies are hampered by the difficulty of handling animals in the field. The aim of this study was to detect genomic DNA of Plasmodium species from the faeces of New World monkeys. Faecal samples from 23 Alouatta clamitans from the Centre for Biological Research of Indaial (Santa Catarina, Brazil) were collected. Extracted DNA from faecal samples was used for molecular diagnosis of malaria by nested polymerase chain reaction. One natural infection with Plasmodium simium was identified by amplification of DNA extracted from the faeces of A. clamitans. Extracted DNA from a captive NHP was also used for parasite genotyping. The detection limit of the technique was evaluated in vitro using an artificial mixture of cultured P. falciparum in NHP faeces and determined to be 6.5 parasites/µL. Faecal samples of New World primates can be used to detect malaria infections in field surveys and also to monitor the genetic variability of parasites and dynamics of infection.


الموضوعات
Animals , Alouatta/parasitology , DNA, Protozoan/genetics , Malaria/veterinary , Monkey Diseases/parasitology , Plasmodium/isolation & purification , Brazil , Feces , Genotype , Malaria/parasitology , Plasmodium/classification
2.
Mem. Inst. Oswaldo Cruz ; 109(5): 608-617, 19/08/2014. tab, graf
مقالة ي الانجليزية | LILACS | ID: lil-720427

الملخص

Plasmodium vivax infects human erythrocytes through a major pathway that requires interaction between an apical parasite protein, the Duffy binding protein (PvDBP) and its receptor on reticulocytes, the Duffy antigen/receptor for chemokines (DARC). The importance of the interaction between PvDBP (region II, DBPII) and DARC to P. vivax infection has motivated our malaria research group at Oswaldo Cruz Foundation (state of Minas Gerais, Brazil) to conduct a number of immunoepidemiological studies to characterise the naturally acquired immunity to PvDBP in populations living in the Amazon rainforest. In this review, we provide an update on the immunology and molecular epidemiology of PvDBP in the Brazilian Amazon - an area of markedly unstable malaria transmission - and compare it with data from other parts of Latin America, as well as Asia and Oceania.


الموضوعات
Humans , Antigens, Protozoan/immunology , Malaria Vaccines/immunology , Malaria, Vivax/prevention & control , Plasmodium vivax/immunology , Protozoan Proteins/immunology , Receptors, Cell Surface/immunology , Antibodies, Protozoan/blood , Antigens, Protozoan/chemistry , Brazil , Enzyme-Linked Immunosorbent Assay , Geography, Medical , Protozoan Proteins/chemistry , Receptors, Cell Surface/chemistry
3.
Mem. Inst. Oswaldo Cruz ; 109(5): 641-653, 19/08/2014. tab, graf
مقالة ي الانجليزية | LILACS | ID: lil-720431

الملخص

Blood infection by the simian parasite, Plasmodium simium, was identified in captive (n = 45, 4.4%) and in wild Alouatta clamitans monkeys (n = 20, 35%) from the Atlantic Forest of southern Brazil. A single malaria infection was symptomatic and the monkey presented clinical and haematological alterations. A high frequency of Plasmodium vivax-specific antibodies was detected among these monkeys, with 87% of the monkeys testing positive against P. vivax antigens. These findings highlight the possibility of malaria as a zoonosis in the remaining Atlantic Forest and its impact on the epidemiology of the disease.


الموضوعات
Animals , Alouatta/parasitology , Malaria/veterinary , Monkey Diseases/epidemiology , Plasmodium/classification , Antibodies, Protozoan/blood , Brazil/epidemiology , Forests , Malaria/epidemiology , Malaria/parasitology , Monkey Diseases/parasitology , Polymerase Chain Reaction
4.
Semina ciênc. agrar ; 28(4): 709-726, out.-dez. 2007. ilus
مقالة ي البرتغالية | LILACS | ID: lil-518358

الملخص

A vacinação com DNA é uma das mais promissoras técnicas de imunização contra uma variedade de patógenos e tumores, para os quais os métodos convencionais não tem sido eficientes. Vacinas de DNA são capazes de induzir resposta imune humoral e celular, tanto para resposta de linfócitos CD4 + quanto CD8 +, sem a necessidade de microrganismos vivos. Apesar do grande potencial de induzir imunidade protetora, a vacina de DNA nem sempre apresenta bons resultados. A imunidade depende de vários fatores como a seleção do gene alvo, construção do vetor de expressão, freqüência e via de administração da vacina, quantidade de DNA, localização do antígeno codificado pelo plasmídio e idade, saúde e espécies de animais vacinados. Esta revisão relata o desenvolvimento de algumas vacinas de DNA para doenças de interesse na medicina veterinária e humana.


The vaccination with DNA is one of the most promising immunization techniques against a pathogensvariety and tumors, for which the conventional methods have not been efficient. DNA vaccines arecapable to induce immune humoral and cellular response, directed to lymphocytes CD4+ and CD8+,without the necessity of live microorganisms. In spite of the great potential of inducing protectiveimmunity, the DNA vaccine not always has success. The immunity depends on several factors such asthe selection of the target gene, construction of the expression vector, frequency and via of administrationof the vaccine, amount of DNA, location of the antigen codified by the plasmid and age, health andspecies of vaccinated animals. This revision shows the development of some vaccines of DNA fordiseases of interest in the veterinary and human medicine.


الموضوعات
Anaplasma marginale , Cattle , Vaccination
اختيار الاستشهادات
تفاصيل البحث