ABSTRACT
Objective To explore the clinical value of indocyanine green (ICG) fluorescence navigation combined with carbon nanoparticles (CNP) in sentinel lymph node biopsy (SLNB) for patients with early breast cancer. Methods A total of 294 early breast cancer patients with axillary node negative in Department of Breast Surgery, Foshan Hospital Affiliated to Sun Yat-Sen University from June 2013 to April 2016 were retrospectively analyzed. Of the patients, 149 cases underwent SLNB with ICG combined with CNP (combination group), while 145 cases underwent SLNB with methylene blue alone (MB group). If the intraoperative pathology results of sentinel lymph nodes (SLNs) were negative, axillary lymph node dissection (ALND) was avoided. The SLNs detection rate, detection number, metastatic SLNs detection rate in SLNB were compared between two groups. The influence of age and body mass index (BMI) on SLNs detection rate was also analyzed. Results In the combination group, subcutaneous lymphatic channels were successfully visualized in 145 patients, and the detection rate was 97.3％(145/149). The fluorescence of SLNs was successfully detected in 143 patients, and the detection rate was 95.9％(143/149). The detection rate of SLNs was higher in the combination group than that of methylene dye alone group (97.9％vs. 91.0％,χ2=6.902,P<0.05). The average number of detected SLNs was higher in the combination group than that of methylene dye alone group (4.5±1.6 vs. 3.2±1.5,t=4.476,P<0.05). Fifty-eight metastatic SLNs were found in 715 SLNs in the combination group (8.1％), and 26 in 544 SLNs in MB group (4.7％). The detection rate was significantly higher in the combination group than that of methylene dye alone group (χ2=13.714,P<0.01). Age and BMI showed no influence on the detection rate and accuracy of SLNB in two groups (P>0.05). Conclusion The combined tracing of ICG fluorescence and carbon nanoparticles for SLNB has showed a better stability and operability in patients with early breast cancer, which is recommended to be a new SLNB method.
ABSTRACT
Objective To study the epidemiological significance of community-structural difference regarding both small mammal and flea communities on Rattus ftavipectus in Dehong and Baoshan areas,Yunnan province,during 1982 to 1996.Methods Methodologies as cluster analysis,communities dominated constitution and species diversity were used for data analysis.Results 75 490 small mammals of 27 species could be divided into three types:Ⅰ.Ruili habitat-communities dominated bv R.flavipectus (54.41%)and Suncus murinus(33.37%): Ⅱ.Longchuan and Yingiiang habitat-communities dominated by R.flavipectus(62.99%),S.murinus(23.25%)and Mus musculus(10.06%);Ⅲ.Baoshan habitat-community dominated by R.flavipectus(48.07%),S.murinus(19.56%)and Crocidura attenuats(14.37%).The captured 61 122 fleas of 11 species on R.flavipectus could be divided into three types: i.Ruili habitat-communities dominated by Xenopsylla cheopis(83.51%)and Lentistivslius ferinus(13.86%);ii.Longchuan and Yingjiang habitats X.cheopis(74.42%)and L.segnis(22.94%); iii.Baoshan habitat-communities dominated by L.segnis(70.62%)and X.cheopis(22.70%).There had been 1471 strains of Y.pestis isolated from the hosts of 7 species and vectors of 5 species in Dehong areas for the ten year period.36 strains of Y.pestis were isolated from host of one species and vectors of 2 species in Baoshan area for ten years.The constitution ratio of R.flavipectus and X.cheopis was higher in Dehong area with average as 58.70%and 78.97%respectively.However.the diversities of species among host and vector communities were low,with average as 1.010 and 0.625.On the contrary,the constitution ratios of R.flavipectus and X.cheopis were lower in Baoshan area.with an average as 48.07%and 22.70%respectively but the diversities of species among host and vector community were higher with the averages as 1.471 and 0.829 respectively.The main dominative flea species from 'group ii'to 'group iii'in the flea community had changed from X.cheopis to L.segnis.Conclusion The species diversity index of host and vector community was higher in Baoshan area,while the constitution ratio of main host and vector community was lower.This findings seemed to be the important factor of the decrease of plague prevalence in Baoshan area.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the selective killing of colorectal tumor cells by lentivirus-mediated double suicide gene under the regulation of KDR promoter.</p><p><b>METHODS</b>293T packaging cells were transfected with the plasmid FGW-KDRP-CD/TK to obtain the infectious viruses. KDR-expressing LoVo cells and LS174T cells that did not produce KDR were transfected with the recombinant virus, and the transfection efficiency was evaluated by the fluorecence microscope. RT-PCR was employed to examine the expression of CDglyTK. After treatment of the cells with 5-FC and GCV, the killing effects on the two cell lines were evaluated.</p><p><b>RESULTS</b>The recombinant construct showed similar infection rate of the two cell lines. RT-PCR demonstrated that CDglyTK gene was expressed only in LoVo cells infected with FGW-KDRP-CD/TK but not in LS147T cells, and the sensitivity of the two cell lines to the prodrugs was significantly different (P<0.001). The killing effect of the double suicide gene was much stronger than that of single suicide gene administered (P<0.001).</p><p><b>CONCLUSION</b>The double suicide gene driven by KDR promoter has specific killing effect on the KDR-expressing colorectal tumor cells.</p>