ABSTRACT
Background: Bone marrow mesenchymal stem cells [BM-MSCs] elicit neuroprotective effects, and their repair ability has been investigated in different experimental models. We aimed to investigate the effect of multiple i.p. BM-MSCs injections in the cuprizone model of multiple sclerosis in mice
Methods: Adult male C57BL/6 mice [n = 40] were fed a regular diet or a diet containing cuprizone [0.2% w/w] for six weeks. Bone marrow samples were taken from patients with spinal cord injury. BM-MSCs [2 * 10[6] in 1 milliliter medium] were administered intraperitoneally for two consecutive weeks at the end of the forth weeks of cuprizone administration. Animals [n = 12] were perfused with 10% paraformaldehyde at the end of sixth week. The brains were sectioned coronally in 6- 8-Mu m thickness [-2.3 to 1.8 mm from bregma]. The sections were stained by luxol fast blue-cresyl violet, and images were captured via a microscope. Demyelination ratio was estimated in corpus callosum in a blind manner. A quantitative real-time PCR was used to measure the myelin basic protein gene expression at sixth week
Results: Histologically, cuprizone induced demyelination in the corpus callosum. Demyelinated area was diminished in the corpus callosum of cell-administered group. Cuprizone could decrease myelin-binding protein mRNAs expression in corpus callosum, which was significantly recovered after BM-MSCs injections
Conclusion: Our data indicated a remyelination potency of multiple i.p. BM-MSCs in the cuprizone model of multiple sclerosis in mice