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1.
Ciênc. rural ; 45(9): 1681-1687, set. 2015. tab
Article in Portuguese | LILACS | ID: lil-756437

ABSTRACT

A produção de cenoura em grande escala na região de Rio Paranaíba coloca essa região em posição de destaque no cenário nacional. No entanto, é relatado que ocorre significativa quantidade de sobras após a colheita. O aproveitamento do material descartado na cadeia de alimentos pode se concretizar pelo processamento mínimo, como uma alternativa de agregação de valor. A etapa de sanitização é de extrema importância durante a produção de vegetais minimamente processados. Nesse contexto, objetivou-se estudar o efeito antimicrobiano de nanopartículas de prata na sanitização de cenoura minimamente processada, obtida a partir do aproveitamento das sobras de cenouras da colheita da cidade de Rio Paranaíba, bem como o estudo da termodinâmica de adesão de diferentes estirpes bacterianas na superfície da cenoura sanitizada. Observou-se que as nanopartículas de prata (6mg L-1)apresentaram bons resultados, quando comparadas aos sanitizantes hipoclorito de sódio (100mg L-1) e dicloroisocianurato de sódio (150mg L-1), na descontaminação da cenoura minimamente processada sobre microrganismos mesófilos aeróbios; Pseudomonasspp.; bactérias láticas; e coliformes a 35ºC. Verificou-se também que a superfície da cenoura apresenta características hidrofílicas que podem dificultar a adesão bacteriana. Esse fato foi confirmado na avaliação de termodinâmica de adesão, que foi desfavorável paraStaphylococcus aureus, Escherichia colie Listeria innocua, sendo mais desfavorável para as interações envolvendo as superfícies de cenoura sanitizadas com nanopartículas de prata.

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The carrot production on a large scale in the region of Rio Paranaiba places the region in a prominent position on the national scene. However, it is reported that a significant amount of scraps occurs after harvest. The use of discarded material in the food chain can be realized by minimal processing, as an alternative of adding value. A sanitization step is very important during the production of minimally processed vegetables. In this context, this research aimed to study the antimicrobial effect of silver nanoparticles in the sanitization of minimal processing carrot from discarded carrot harvested at Rio Paranaiba city, as well as study the thermodynamics adhesion for different bacterial strains in sanitized carrot surface. It was observed that silver nanoparticles (6mg L-1) showed good results when was compared to sodium hypochlorite (100mg L-1) and sodium dichloroisocyanurate (150mg L-1) in reducing populations of aerobic mesophilic microorganism;Pseudomonasspp. lactic acid bacteria and coliforms at 35ºC present in carrot. It was also found that the surface of carrot has hydrophilic characteristics which can prevent bacterial adhesion. This fact was confirmed that thermodynamic evaluation of adhesion that was unfavorable for Staphylococcus aureus, Escherichia coliand Listeria innocua, and more thermodynamically unfavorable for interactions involving carrot surfaces sanitized with silver nanoparticles.

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2.
Ciênc. rural ; 44(11): 2090-2094, 11/2014. tab
Article in Portuguese | LILACS | ID: lil-728726

ABSTRACT

A indústria de leite e derivados no Brasil lança todos os anos diversos novos produtos no mercado, incluindo produtos de alto valor nutricional e tecnologia de fabricação de baixo custo. Focando neste tema, foi avaliado o efeito do envase a quente de uma bebida a base de soro de leite tratada termicamente após fermentação, e sua conservação à temperatura ambiente, por 84 dias. Durante o processamento, utilizou-se um tratamento térmico brando, aliado ao baixo pH, para que a bebida pudesse ser armazenada a temperatura ambiente. Garrafas PET de 250 mL foram utilizadas para o envase a quente do produto. Para avaliar sua estabilidade durante o armazenamento, foram realizadas periodicamente análises físico-químicas, microbiológicas e análise sensorial. Os resultados obtidos foram analisados por análise de variância e teste de Tukey para comparação de médias. Análises físico-químicas e microbiológicas indicaram que a bebida manteve-se estável; entretanto, o sabor foi alterado ao longo do armazenamento. O baixo custo do processamento e embalagem são benefícios do produto avaliado, podendo-se concluir que a bebida é uma boa forma para a utilização do soro de leite. Uma grande vantagem deste produto é o seu armazenamento à temperatura ambiente, dispensando o uso da refrigeração durante o transporte e acondicionamento em supermercados.


The Brazilian dairy industry launches several new products on the market every year, including products with high nutritional value and low-cost manufacturing technology. Focusing on this issue, a cheese whey beverage thermally treated after fermentation was hot-filled and evaluated for 84 days. During the beverage manufacturing, we applied a mild heat treatment in combination with low pH aiming to store the beverage at room temperature. PET bottles were used to hot filling of the product. To assess the product stability during the storage period, we performed, periodically, physico-chemical, microbiological and sensory analysis. The obtained results were analyzed by the Analysis of Variance and Tukey's test. Physical-chemical and microbiological analysis indicated that the beverage remained stable; however, the flavor was altered during the storage period. Since hot-filling of dairy beverages in PET bottles is a low cost-effective procedure easily deployed in dairy plants, this process allows the productive use of cheese whey. A remarkable advantage of such product is its possible storage at room temperature, reducing distribution and sales expenditures with cooling.

3.
Biosci. j. (Online) ; 29(2): 506-515, mar./apr. 2013. tab, ilus
Article in Portuguese | LILACS | ID: biblio-914421

ABSTRACT

Sanitizantes químicos são utilizados na higienização de superfícies que entram em contato com os alimentos, de forma a eliminar patógenos e reduzir deterioradores assegurando a inocuidade dos alimentos para os consumidores. Atualmente, tem-se o interesse no desenvolvimento de novos antimicrobianos, em razão do aumento no número de bactérias resistentes. Neste contexto, o objetivo deste trabalho foi otimizar a produção de nanopartículas de prata a partir de uma nova síntese, bem como avaliar sua capacidade antimicrobiana por diferentes metodologias. Observou-se que o rendimento na produção das nanopartículas de prata aumentou à medida que a concentração do surfactante usado na dispersão foi também aumentada, isto pode ser explicado pelo fato da reação de formação das nanopartículas se caracterizar como uma catálise micelar. Pelos resultados obtidos no teste de adesão bacteriana, observouse que a adesão de Staphylococcus aureus e Listeria innocua foi menor nas superfícies condicionadas com as nanopartículas de prata. As nanopartículas de prata também foram capazes de reduzir o número de bactérias aderidas em diferentes superfícies de aço inoxidável, superfícies estas, comumente utilizadas nas indústrias de alimentos. Portanto, as nanopartículas de prata obtidas por uma nova síntese apresentam-se eficientes como agentes antimicrobianos, com potencial uso como sanitizantes, na indústria de alimentos.


Sanitizers are used to clean surfaces that come in contact with food in order to eliminate pathogens and reduce spoilage ensuring food safety for consumers. Actually, there is interest in developing new antimicrobial agents, due to the increase in the number of resistant bacteria. In this context, the work aimed to optimize the production of silver nanoparticles obtained by a new synthesis, and assess their antimicrobial ability. We observed that the yield in production of the silver nanoparticles increases as is also increased the concentration of surfactant used in the dispersion, since the reaction produced is characterized in a micellar catalyst. Results obtained during testing of bacterial adhesion showed us that the adherence of S. aureus and L. innocua was lower when the surfaces were covered with the silver nanoparticles. The nanoparticles were also able to reduce the number of attached bacteria on surfaces of stainless steel. Therefore, the silver nanoparticles obtained by a new synthesis have to be effective antimicrobial agents with the potential use as sanitizing in the food industry.


Subject(s)
Silver , Food Contamination , Nanoparticles , Anti-Infective Agents
4.
Braz. j. microbiol ; 43(4): 1261-1268, Oct.-Dec. 2012. tab
Article in English | LILACS | ID: lil-665807

ABSTRACT

The adhesion of the solids presents in food can difficult the process of surface cleaning and promotes the bacterial adhesion process and can trigger health problems. In our study, we used UHT whole milk, chocolate based milk and infant formula to evaluate the adhesion of Enterobacter sakazakii on stainless steel coupons, and we determine the work of adhesion by measuring the contact angle as well as measured the interfacial tension of the samples. Inaddition we evaluated the hydrophobicity of stainless steel after pre-conditioning with milk samples mentioned. E. sakazakii was able to adhere to stainless steel in large numbers in the presence of dairy products. The chocolate based milk obtained the lower contact angle with stainless steel surface, higher interfacial tension and consequently higher adhesion work. It was verified a tendency of decreasing the interfacial tension as a function of the increasing of protein content. The pre-conditioning of the stainless steel coupons with milk samples changed the hydrophobic characteristics of the surfaces and became them hydrophilic. Therefore, variations in the composition of the milk products affect parameters important that can influence the procedure of hygiene in surface used in food industry.


Subject(s)
Humans , Bacterial Adhesion , Cronobacter sakazakii/isolation & purification , Enterobacteriaceae Infections , Hydrophobic and Hydrophilic Interactions , Dairy Products/analysis , Food Samples , Methods , Milk
5.
Braz. j. microbiol ; 41(4): 984-992, Oct.-Dec. 2010. graf, tab
Article in English | LILACS | ID: lil-595739

ABSTRACT

The interaction between the surface of stainless steel and Bacillus cereus was studied in terms of the characteristics of interfacial interaction determined from the measurement of the contact angle of the surface of B. cereus and stainless steel in the presence or absence of B. cereus adherence. The microtopographies and the roughness of the surface of stainless steel and stainless steel adhered by B. cereus were evaluated with the help of atomic force microscopy and perfilometry. The strain of B. cereus studied was considered hydrophilic, whereas the stainless steel was considered hydrophobic. The adhesion was not thermodynamically favorable (ΔGadhesion > 0) between the stainless steel and the strain of B. cereus studied. Thus, the interaction between them was not favored by the thermodynamic aspect of adhesion. There was no difference (p > 0.05) in the roughness of the surfaces of stainless steel adhered by B. cereus when analyzed by atomic force microscope and perfilometry.

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