CCR3 antagonist impairs estradiol-induced eosinophil migration to the uterus in ovariectomized mice

Eosinophils are abundant in the reproductive tract, contributing to the remodeling and successful implantation of the embryo. However, the mechanisms by which eosinophils migrate into the uterus and their relationship to edema are still not entirely clear, since there are a variety of chemotactic factors that can cause migration of these cells. Therefore, to evaluate the role of CCR3 in eosinophil migration, ovariectomized C57BL/6 mice were treated with CCR3 antagonist SB 328437 and 17β-estradiol. The hypothesis that the CCR3 receptor plays an important role in eosinophil migration to the mouse uterus was confirmed, because we observed reduction in eosinophil peroxidase activity in these antagonist-treated uteruses. The antagonist also influenced uterine hypertrophy, inhibiting edema formation. Finally, histological analysis of the orcein-stained uteruses showed that the antagonist reduced eosinophil migration together with edema. These data showed that the CCR3 receptor is an important target for studies that seek to clarify the functions of these cells in uterine physiology.

Análisis del gasto de bolsillo ante la enfermedad de personas de 60 y más años según su condición de pobreza en Paraguay

Introducción: Reducir la pobreza y lograr cobertura universal de salud con protección financiera para las personas de todas las edades son parte de los Objetivos de Desarrollo Sostenible. Objetivo: Determinar el gasto de bolsillo de salud (GBS) ante la enfermedad de personas de 60 y más años (≥ 60 años) y su incidencia económica en los hogares según su condición de pobreza en Paraguay. Material y Método: Se analizó la Encuesta Permanente de Hogares del 2014, considerando la definición oficial de pobreza y la referencia de enfermedad o accidente en un trimestre. Se calculó el promedio de GBS por hogar, la incidencia sobre el ingreso monetario trimestral (IM) y sobre los subsidios estatales por pensión alimentaria para adultos mayores (IPa). Resultados: De los 1.621.525 hogares del país 435.448 tenía al menos 1 persona ≥ 60 años, de los cuales 188.715 tenía al menos 1 de ellos enfermo o accidentado y 144.554 reportó GBS. Entre los hogares no pobres: 11,6% (IC 10,5 a 12,9) tuvo al menos 1 enfermo ≥ 60 años, de los cuales 75,8% (IC 71,2 a 79,8) tuvo GBS, siendo en promedio 547.931 Gs. (DE 58.251). Los medicamentos fueron 53,3% (IC 45,2 a 61,5) del total. La IM fue 5,7% (IC 4,6 a 6,9). Entre los hogares pobres: 11,6% (IC 9,6 a 14,0) tuvo al menos 1 enfermo ≥ 60 años, de los cuales 79,5% (IC 70,7 a 86,2) tuvo GBS, siendo en promedio 341.542 Gs. (DE 49.101). Los medicamentos fueron 71,3% (IC 61,9 a 80,7) del total. La IM fue 11,5% (IC 7,0 a 16,0), la IPa fue de 16,9% (IC 4,4 a 29,4). El GBS fue el equivalente a 19 días de alimentación en promedio (DE 3,71). Conclusión: Paraguay requiere de mayor protección financiera para adultos mayores mediante sinergias de las políticas de reducción de pobreza y de salud.

Introduction: Reducing poverty and achieving universal health coverage for people of all ages are part of the Sustainable Development Goal. Objective: determine the Out-of-pocket Health Expenditure (GBS) for people 60 and older (≥ 60 years) who are sick and its economic impact on households according to their poverty status in Paraguay. Material and Method: the 2014 Permanent Household Survey was analyzed, considering the official definition of poverty and the reference of illness or accident in a quarter. The average of GBS per household, the impact on quarterly monetary risk (IM) and on state subsidies for food pensions for the elderly (IPa). Results: Of 1,621,525 homes in the country 435,448 had at least 1 person 60 years, of which 188,715 had at least 1 of them ill or injured and 144,554 reported GBS. Among the non-poor households: 11.6% (IC 10.5 to 12.9) had at least 1 ill person ≥ 60 years, of which 75.8% (IC 71.2 to 79.8) had GBS, being on average 547,931 Gs. (DE 58,251). The medications were 53.3% (IC 45.2 to 61.5) from total. There was an IM of 5,7% (IC 4.6 to 6.9). Among the poor households: 11.6% (IC 9.6 to 14.0) had at least 1 ill person ≥ 60 years, of which 79.5% (IC 70.7 to 86,2) had GBS, being on average 341,542 Gs. (DE 40,101). The medications were 71.3 % (IC 61.9 to 80.7) from total. There was an IM of 11.5% (IC 7.0 to 16.0). There was an IPa of 16.9% (IC 4.4 to 29.4). The GBS was the equivalent to 19 days of feeding on average (DE 3.71). Conclusion: Paraguay requires more financial protection for older adults through synergies of poverty reduction and health policies.

Agonistic activity of tamoxifen, a selective estrogen-receptor modulator (SERM), on arthritic ovariectomized mice

Arthritis is positively associated with the decline of sex hormones, especially estrogen. Tamoxifen (TMX) is a selective estrogen receptor modulator, possessing agonist or antagonistic activity in different tissues. Thus, the objective of this study was to investigate the effect of TMX on the zymosan-induced arthritis model. Female Swiss normal and ovariectomized (OVX) mice were divided into groups and treated for five days with TMX (0.3, 0.9 or 2.7 mg/kg) or 17-β-estradiol (E2, 50 µg/kg). On the fifth day, arthritis was induced and 4 h later, leukocyte migration into joint cavities was evaluated. The neutrophil migration in OVX animals, but not in normal mice, treated with TMX (all tested doses) was significantly decreased compared with mice that received the vehicle (P≤0.05). Similarly, this effect was also demonstrated in the E2-treated group. Therefore, the present study demonstrates that TMX presented agonist effects in inhibiting neutrophil migration and preventing arthritis progression in OVX mice.

Physiological response of invasive mussel Limnoperna fortunei (Dunker, 1857) (Bivalvia: Mytilidae) submitted to transport and experimental conditions / Resposta fisiológica do mexilhão invasor Limnoperna fortunei (Dunker, 1857) (Bilvalvia: Mytilidae) submetido ao transporte e condições experimentais

Abstract Successful animal rearing under laboratory conditions for commercial processes or laboratory experiments is a complex chain that includes several stressors (e.g., sampling and transport) and incurs, as a consequence, the reduction of natural animal conditions, economic losses and inconsistent and unreliable biological results. Since the invasion of the bivalve Limnoperna fortunei (Dunker, 1857) in South America, several studies have been performed to help control and manage this fouling pest in industrial plants that use raw water. Relatively little attention has been given to the laboratory rearing procedure of L. fortunei, its condition when exposed to a stressor or its acclimation into laboratory conditions. Considering this issue, the aims of this study are to (i) investigate L. fortunei physiological responses when submitted to the depuration process and subsequent air transport (without water/dry condition) at two temperatures, based on glycogen concentrations, and (ii) monitor the glycogen concentrations in different groups when maintained for 28 days under laboratory conditions. Based on the obtained results, depuration did not affect either of the groups when they were submitted to approximately eight hours of transport. The variation in glycogen concentration among the specimens that were obtained from the field under depurated and non-depurated conditions was significant only in the first week of laboratory growth for the non-depurated group and in the second week for the depurated group. In addition, the tested temperature did not affect either of the groups that were submitted to transport. The glycogen concentrations were similar to those of the specimens that were obtained from the field in third week, which suggests that the specimens acclimated to laboratory conditions during this period of time. Thus, the results indicate that the air transport and acclimation time can be successfully incorporated into experimental studies of L. fortunei. Finally, the tolerance of L. fortunei specimens to the stressor tested herein can help us understand the invasive capacity of this mussel during the establishment process.

Resumo A criação bem sucedida de animais em condições de laboratório para processos comerciais ou experimentais é uma cadeia complexa que inclui vários fatores de estresse (ex. coleta e transporte) que tem como consequência a redução das condições naturais do animal, prejuízos econômicos e resultados biológicos inconsistentes. Desde a invasão do bivalve Limnoperna fortunei (Dunker, 1857) na América do Sul, vários estudos têm sido realizados para ajudar no controle e gestão dessa praga em plantas industriais que utilizam água. Relativamente pouca atenção tem sido dada ao processo de criação de L. fortunei em laboratório, sua condição quando exposta ao estresse e sua aclimatação a condições de laboratório. Considerando estes aspectos, os objetivos deste estudo foram: (i) investigar as respostas fisiológicas de L. fortunei submetidos ao processo de depuração e subsequente transporte (sem água/condição seca) em duas temperaturas, analisando as diferentes concentrações de glicogênio e (ii) monitorar as concentrações de glicogênio nos diferentes grupos, quando mantidos por 28 dias em condições de laboratório. Com base nos resultados obtidos, a depuração não afetou nenhum grupo quando eles foram submetidos a oito horas de transporte. A variação da concentração de glicogênio entre os espécimes do campo quando depurados e não depurados, foi significativa apenas em relação à primeira semana em laboratório para o grupo não depurado e à segunda semana para o grupo depurado. Além disto, a temperatura testada não afetou os grupos submetidos ao transporte. As concentrações de glicogénio foram semelhantes as dos espécimes do campo a partir da terceira semana, o que sugere que os espécimes estão aclimatados às condições de laboratoriais neste período de tempo. Assim, os resultados indicam que o transporte ao ar e o tempo de aclimatação podem ser incorporados com sucesso aos estudos experimentais com L. fortunei. Finalmente, o conhecimento sobre a tolerância de L. fortunei ao estresse pode ajudar a entender a capacidade invasiva deste durante o processo de estabelecimento.

Atividades antioxidante e antimicrobiana de Ziziphus joazeiro mart. (Rhamnaceae): avaliação comparativa entre cascas e folhas / Antioxidant and antimicrobial activities of Ziziphus joazeiro mart. (Rhamnaceae): comparison between bark and leaves

Os objetivos deste estudo foram determinar as atividades antioxidante e antimicrobiana de extratos de cascas e folhas de uma notável árvore da Caatinga (semi-árido brasileiro), usado em remédios populares, Ziziphus joazeiro Mart. A atividade antioxidante foi avaliada através do método de captação pelo DPPH, enquanto a atividade antimicrobiana foi avaliada pelo método de difusão em ágar seguido pela determinação da concentração inibitória mínima (CMI). Os resultados mostraram que ambos os extratos de Z. joazeiro possuem atividade antioxidante com EC50 de 461,88 e 1.743,05mg/mL para as folhas e casca, respectivamente. As amostras foram ativas contra 70% das bactérias testadas. O extrato de folhas mostrou CMI entre 0,25-0,5 mg/mL contra Micrococcus luteus e entre 0,125-0,250 mg/mL contra Mycobacterium smegmatis, enquanto o extrato da casca apresentou CMI entre 0,5-1,0 mg /ml M. smegmatis.

The objectives of this study were to determine the antioxidant and antimicrobial activities of ethanolic extracts of the bark and leaves of a notable tree of the caatinga (Brazilian semiarid scrubland) used in many popular remedies, Ziziphus joazeiro Mart. The antioxidant activity was assessed by the DPPH free-radical scavenging assay, while the antimicrobial activity, against a range of Gram positive and negative bacteria and a yeast, was determined by the agar disk diffusion method, followed by an assay of the minimum inhibitory concentration (MIC). The results showed that both extracts of Z. joazeiro have antioxidant activity, the EC50 of leaf extract being 461.88 and that of bark 1743.05 mg/mL, with respect to DPPH absorbance in a standard assay. Samples were active against 70% of the bacterial species tested. The leaf extract showed MICs of 0.25-0.5 mg/mL against Micrococcus luteus and 0.125-0.250 mg/mL against Mycobacterium smegmatis, while the bark extract showed a MIC of 0.5-1.0 mg/mL against M. smegmatis.

Ensaio para detectar bergapteno na casca e no caule de Brosimum gaudichaudii Trec através da produção de melanina em actinomicetos / A new assay for detection of furocoumarins in Brosimum gaudichaudii Trec by melanin production in actinomycetes

Plant extracts containing furocoumarins compounds were identified by using melanin producer strains of Nocardiopis sp (DAUFPE-361) and non producer strains of Streptomyces sp (DAUFPE-87). Ethanol, methanol and acetone extracted compounds from conduru (Brosimum gaudichaudii) bark and stem were evaporated and added to tubes containing ISP7 medium (tyrosine agar). This medium was inoculated with spore suspension (10(6)-10(7) CFU) prepared from the different actinomycetes strains. The ethanol fraction (pH 9.0) from both bark and stem induced pigmentation in actinomycetes melanin non producer strains and increased the pigmentation in producer strains indicating presence of furocoumarins in the correspondent plant extracts.