ABSTRACT
Solanum trilobatum Linn is a medicinal plants used in India from many centuries to cure various diseases. The purpose of the study is to evaluate the ameliorative potential of the ethanolic leaf extract and fractions of Solanum trilobatum [St] against alloxan induced diabetic rats. Solanum trilobatum leaf extract and fractions were administered orally at two different concentration [100-200mg/kg body weight] to alloxan induced diabetic rats. The antidiabetic efficacy was validated through various biochemical parameters, enzyme assays, renal markers and antioxidant properties were also determined. The phytochemical analyses of St leaves were done by estimating their Chlorophyll, carotenoids, total sugar, protein, amino acid and minerals contents. The results revealed that the leaf extracts and fractions of St were efficient anti hyperglycemic agents and posses potent nephro-protective activity. However, the extracts of St leaves at a concentration of 200mg/kg bw exhibit higher efficacy in all tested concentrations. From the result it can be concluded that the leaf extracts of St can be a potential candidate in treating the hyperglycemic conditions and justifies its use in ethano medicine and can be exploits in the management of diabetes
ABSTRACT
Monochoria vaginalis is an herbaceous medicinal plant used to treat, liver problems India. Acetaminophen is a commonly used analgesic and antipyretic agent which, at high doses, causes liver and kidney necrosis in man and animals. The aim of the present study is to evaluate phytoconstituents and investigate the nephroprotective and antioxidant activities of the ethanol extract of Monochoria vaginalis on acetaminophen induced toxicity in rats. Phytoconstituents like n-hexadecanoic acid, 3-methyl- acetate-1-butanol, 1,1,3-triethoxy- propane, Z,Z,Z-1,4,6,9 - nonadecatetraene, undecanoic acid, 3-trifluoroacetoxy penta decane and 4-ethyl-5-octyl-2,2-bis [trifluoromethyl] - cis-l,3-dioxalone were identified from ethanol extract of Monochoria vaginalis by using a gas chromatograph-mass spectrograph [GC MS]. Biochemical studies show that there is an increase in the levels of serum urea and creatinine along with an increase in the body weight and reduction in the levels of uric acid in acetaminophen induced groups. These values are retrieved significantly by treatment with Monochoria vaginalis extracts at two different doses. The antioxidant studies reveal that the levels of renal SOD, CAT, GSH and GPx in the APAP treated animals are increased significantly along with a reduced MDA content in ethanol extract of Monochoria vaginalis treated groups. Apart from these, histopathological changes also reveal the protective nature of the Monochoria vaginalis extract against acetaminophen induced necrotic damage of renal tissues. In conclusion, these data suggest that the ethanol extract of Monochoria vaginalis can prevent renal damage from APAP induced nephrotoxicity in rats and it is likely to be mediated through active phytoconstituents and its antioxidant activities