ABSTRACT
Objective:To preliminarily interpret the compatibility of Ginseng Radix et Rhizoma Rubra and Rhodiolae Crenulate Radix et Rhizoma in chemical and pharmacodynamic levels，and provide theoretical basis for its clinical application.Method:Rapid resolution liquid chromatography coupled with quadrupole-time-of-flight tandem mass spectrometry（RRLC-Q-TOF-MS） was applied to identify and analyze the changes in chemical components of the Ginseng Radix et Rhizoma Rubra and Rhodiolae Crenulate Radix et Rhizoma before and after compatibility. The anti-fatigue activity before and after compatibility of Ginseng Radix et Rhizoma Rubra and Rhodiolae Crenulate Radix et Rhizoma was detected by weight-loading swimming experiment and determination of levels of serum urea，blood lactic acid and hepatic glycogen.Result:A total of 51 compounds were identified in mixture decoction of Ginseng Radix et Rhizoma Rubra and Rhodiolae Crenulate Radix et Rhizoma. Malonyl ginsenoside mRg1，mRb1，mRb2，mRb3 and mRd contents were significantly decreased，while ginsenoside Rb1，Rb2，Rb3，Rd，F2 and Rg3 contents were significantly increased in the compatibility mixture. According to pharmacodynamics study，as compared with those in the blank control group，swimming time of mice was significantly prolonged in all other groups （P<0.01），serum urea nitrogen（P<0.05，P<0.01） and lactic acid（P<0.05，P<0.001） levels of mice in the combined decoction and the single decoction groups were significantly lowered，while liver glycogen levels were significantly elevated（P<0.05，P<0.01）. The anti-fatigue ability of the combined decoction of Ginseng Radix et Rhizoma Rubra and Rhodiolae Crenulate Radix et Rhizoma was higher than that of the single decoctions.Conclusion:In this article, the effect enhancing mechanism of compatibility of Ginseng Radix et Rhizoma Rubra with Rhodiolae Crenulate Radix et Rhizoma was revealed based on the chemical changes, providing theoretical reference for the clinical application and development of products.
ABSTRACT
The use of animal medicine has a long history in China, it has the characteristics of high curative effect,strong activity, wide application and great potential. However,the circulation of animal medicine in current market mixed counterfeit variety and complex. Molecular identification technology of DNA barcoding is an emerging molecular biotechnology in recent years, it is a powerful supplement to traditional identification methods. This method can well identify animal species at the molecular level and has high accuracy, it can identify animal medicines quickly and monitor the medicine market effectively. This article summarizes the research process of molecular identification of DNA barcoding, the application of DNA barcoding in medicinal animals identification in recent years, and the limitations of DNA barcoding technology.