Effects of sports training associated with nutritional intervention on athletes' post-competition recovery / Efeitos do treinamento esportivo associado à intervenção nutricional sobre a recuperação dos atletas pós-competição / Efectos del entrenamiento deportivo asociado a una intervención nutricional en la recuperación post competición de los deportistas

ABSTRACT Introduction: The athletes' physiological capacity and psychological level are raised to the limit during the competition, which is necessary for adequate recovery after the dispute. Objective: Study the effect of sports training associated with nutritional intervention on athletes' post-competition recovery. Methods: In the first two weeks of the experiment, training was done in slow aerobic exercise, aerobics, and running. Strength training and a small amount of anaerobic training were added to the training with the gradual practice of the experiment. Results: The proportion of breakfast, lunch, and dinner was 25.33%, 42.55%, and 32.12%, respectively; protein intake decreased from 34.23% to 20.13%, and the proportion of fat intake decreased from 15. 56% to 12.3%, and IgG content changed from 7.654 ± 0.283 (mmol/L) at the end to 8.586 ± 0.651 (mmol/L); IgM content changed from 3.382 ± 0.875 (g/L) at the end to 3.169 ± 1.002 (g/L). Conclusion: Athletes can effectively, through sports training and nutritional intervention, adjust body changes caused by competition while maintaining a good exercise level. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução: A capacidade fisiológica e o nível psicológico dos atletas são elevados ao limite durante a competição, sendo necessária uma recuperação adequada após a disputa. Objetivo: Estudar o efeito do treinamento esportivo associado à intervenção nutricional para a recuperação dos atletas após a competição. Métodos: Nas duas primeiras semanas da experiência, realizou-se o treinamento na forma de exercício aeróbico lento, aeróbica e corrida. O treinamento de força e uma pequena quantidade de treinamento anaeróbico foram adicionados ao treinamento com a prática gradual do experimento. Resultados: A proporção de café da manhã, almoço e jantar foi de 25,33%, 42,55% e 32,12% respectivamente, a proporção de ingestão de proteínas diminuiu de 34,23% para 20,13%, a proporção de ingestão de gordura diminuiu de 15,56% para 12,3%, e o conteúdo de IgG mudou de 7,654 ± 0,283 (mmol/L) no final para 8,586 ± 0,651 (mmol/L); o conteúdo de IgM mudou de 3,382 ± 0,875 (g/L) no final para 3,169 ± 1,002 (g/L). Conclusão: Os atletas podem de maneira eficaz, através de treinamento esportivo e intervenção nutricional, ajustar as alterações corporais ocasionadas pela competição, mantendo um bom nível de exercício. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción: La capacidad fisiológica y el nivel psicológico de los atletas se elevan al límite durante la competición, siendo necesaria una adecuada recuperación tras la disputa. Objetivo: Estudiar el efecto del entrenamiento deportivo asociado a la intervención nutricional para la recuperación de los deportistas tras la competición. Métodos: En las dos primeras semanas del experimento, el entrenamiento se realizó en forma de ejercicio aeróbico lento, aeróbic y carrera. El entrenamiento de fuerza y una pequeña cantidad de entrenamiento anaeróbico se añadieron al entrenamiento con la práctica gradual del experimento. Resultados: La proporción de desayuno, comida y cena fue del 25,33%, 42,55% y 32,12% respectivamente, la proporción de ingesta de proteínas descendió del 34,23% al 20,13%, la proporción de ingesta de grasas descendió del 15. 56% a 12,3%, y el contenido de IgG pasó de 7,654 ± 0,283 (mmol/L) al final a 8,586 ± 0,651 (mmol/L); el contenido de IgM pasó de 3,382 ± 0,875 (g/L) al final a 3,169 ± 1,002 (g/L). Conclusión: Los atletas pueden ajustar eficazmente, mediante el entrenamiento deportivo y la intervención nutricional, los cambios corporales provocados por la competición, manteniendo un buen nivel de ejercicio. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Analysis of the effects of high-intensity strength training on musculature and metabolism in college students / Análise dos efeitos do treinamento de força de alta intensidade sobre a musculatura e o metabolismo de estudantes universitários / Análisis de los efectos del entrenamiento de fuerza de alta intensidad sobre la musculatura y el metabolismo en estudiantes universitarios

ABSTRACT Introduction: Recent studies shows that college students have decreased physical fitness and increased obesity rates, and the rate of obesity will continue to increase over time. Objective: Study the effect of high-intensity strength training on college students' muscle optimization and metabolic recovery. Methods: This paper adopts the experimental control method. An experimental group performed high-intensity strength training and completed the training with the help of the existing equipment in our school's gym. The control group used normal aerobic training to complete the running training and other movements in athletics. The experimental and control groups were trained three times a week for one hour at a time. Results: In the experimental group, left arm elbow joint extension was optimized to (18.405 ± 2.8878) kg after the experiment, P<0.05; right leg knee joint extension was optimized to (38.754 ± 6.6556) kg after the experiment, P<0.01; serum total cholesterol was optimized to (3.682 ± 0.2643) mmol/L after the experiment, P<0.01. Conclusion: High-intensity strength training, when compared with ordinary aerobic training, has a better optimizing effect on college students' indicators and higher exercise efficiency. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução: Segundo consta nas pesquisas recentes, os estudantes universitários têm diminuído a aptidão física e aumentado a taxa de obesidade, e o índice de obesidade permanecerá aumentando com o tempo. Objetivo: Estudar o efeito do treinamento de força de alta intensidade sobre a otimização muscular e a recuperação metabólica dos estudantes universitários. Métodos: Este trabalho adota o método experimental de controle. Um grupo experimental realizou o treinamento de força de alta intensidade e completou o processo de treinamento com a ajuda do equipamento existente na academia da nossa escola. O grupo de controle usou o treinamento aeróbico normal para completar o treino de corrida e outros movimentos no atletismo. O grupo experimental e o grupo de controle foram treinados três vezes por semana durante uma hora de cada vez. Resultados: No grupo experimental, a extensão da articulação do cotovelo do braço esquerdo foi otimizada para (18,405 ± 2,8878) kg após o experimento, P<0,05; a extensão da articulação do joelho da perna direita foi otimizada para (38,754 ± 6,6556) kg após o experimento, P<0,01; o colesterol total sérico foi otimizado para (3,682 ± 0,2643) mmol/L após o experimento, P<0,01. Conclusão: O treinamento de força de alta intensidade, quando comparado com o treinamento aeróbico comum, tem um melhor efeito de otimização nos indicadores dos estudantes universitários e uma maior eficiência no exercício. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción: Según las investigaciones recientes, los estudiantes universitarios presentan una disminución de la forma física y un aumento de la tasa de obesidad, y la tasa de obesidad seguirá aumentando con el tiempo. Objetivo: Estudiar el efecto del entrenamiento de fuerza de alta intensidad sobre la optimización muscular y la recuperación metabólica en estudiantes universitarios. Métodos: Este trabajo adopta el método de control experimental. Un grupo experimental realizó el entrenamiento de fuerza de alta intensidad y completó el proceso de entrenamiento con la ayuda del equipamiento existente en el gimnasio de nuestro colegio. El grupo de control utilizó el entrenamiento aeróbico normal para completar el entrenamiento de carrera y otros movimientos en atletismo. El grupo experimental y el grupo de control se entrenaron tres veces por semana durante una hora cada vez. Resultados: En el grupo experimental, la extensión de la articulación del codo del brazo izquierdo se optimizó a (18,405 ± 2,8878) kg después del experimento, P<0,05; la extensión de la articulación de la rodilla de la pierna derecha se optimizó a (38,754 ± 6,6556) kg después del experimento, P<0,01; el colesterol total sérico se optimizó a (3,682 ± 0,2643) mmol/L después del experimento, P<0,01. Conclusión: El entrenamiento de fuerza de alta intensidad, en comparación con el entrenamiento aeróbico ordinario, tiene un mejor efecto optimizador sobre los indicadores de los estudiantes universitarios y una mayor eficacia del ejercicio. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Chlamydia trachomatis pORF5 protein inhibits apoptosis through activating PI3K-Akt pathway / 中国免疫学杂志

Objective:To study the relationship between apoptosis and the pORF5 protein of Chlamydia trachomatis,and further to explore its molecular mechanisms,which could lay a foundation for chlamydial pathogenic mechanisms.Methods:pGEX-6p/pORF5 recombinant expression vector was transformed to XL1-blue E.Coli to express GST-pORF5 fusion protein,and GST-pORF5 fusion protein was purified with Glutathione Sepharose 4B Beads,and cleaved to get pORF5 protein without GST tag by PreScission protease.The pORF5 protein was used to stimulate HeLa cells at different concentrations,then Western blot was used to evaluate the ex-pression of Bax,Bcl-2 and phosphorylation of PI3K/Akt at different time points,Hoechst staining and Flow cytometry were applied to measure the apoptosis of HeLa cells.Before treated with pORF5 protein for 24 h,HeLa cells were pretreated with PI3K inhibitor LY294002 for 1 h,the expression of Bax,Bcl-2 and the phosphorylation of Akt were evaluated by Western blot,apoptosis rates were also determined.Results:The pORF5 protein changed the expression of Bax and Bcl-2 in dose-and time-dependent manners,pORF5 increased the expression of Bcl-2 and decreased the expression of Bax at the concentration of 10 μg/ml,and there was obvious change at concentration of 15 μg/ml for 24 h.The apoptosis rates of pORF5 treated group were reduced by 27.3% and 8.4% respectively when compared with TNF-α treated group(P<0.01) and untreated group (P<0.05).Akt was phosphorylated after stimulated with pORF5 protein for 15 min,and reached its peak at 30 min.PI3K/Akt inhibitor led to the decrease of the expression of Bcl-2 and phosphorylation of Akt and increase of the expression of Bax,furthermore,PI3K/Akt inhibitor reversed pORF5-mediated anti-apoptosis, the apoptosis rate in LY294002 treated group was increased by 13.0%,when compared with the control group(P<0.01).Conclusion:pORF5 protein could inhibit apoptosis through activating PI3K/Akt signal pathway by induction of Bcl-2 and suppression of Bax.

New Parametric Imaging Method with Fluorescein Angiograms for Detecting Areas of Capillary Nonperfusion / 대한의료정보학회지

OBJECTIVES: Fluorescein angiography (FAG) is currently the most useful diagnostic modality for examining retinal circulation, and it is frequently used for the evaluation of patients with diabetic retinopathy, occlusive diseases, such as retinal venous and arterial occlusions, and wet macular degeneration. This paper presents a method for objectively evaluating retinal circulation by quantifying circulation-related parameters. METHODS: This method allows the semiautomatic preprocessing and registering of FAG images. The arterial input function is estimated from the registered set of FAG images using gamma-variate fitting. Then, the parameters can be computed by deconvolution on the basis of truncated singular value decomposition, and they can finally be presented as parametric color images in a combination of three colors, red, green, and blue. RESULTS: After the estimation of arterial input function, the parameters of relative blood flow and mean transit time were computed using deconvolution analysis based on truncated singular value decomposition. CONCLUSIONS: The parametric color image is helpful to interpret the status of retinal blood circulation and provides quantitative data on retina ischemia without interobserver variability. This system easily provides the status of retinal blood circulation both qualitatively and quantitatively. It also helps to standardize FAG interpretation and may contribute to network-based telemedicine systems in the future.

A Study of Joint Space Narrowing and Erosion in Rheumatoid Arthritis / 대한의료정보학회지

OBJECTIVE: This study was conducted to measure radiographic joint space width and to estimate erosion in the hands of patients with rheumatoid arthritis. It showed that joint space width, homogeneity, and invariant moments are parameters to discriminate between the normal and the rheumatoid joint. METHODS: In order to measure the joint space width and to estimate erosion in the finger joint, 32 radiographic images were used - 16 images for training and 16 images for testing. The joint space width was measured in order to quantify the joint space narrowing. Also, homogeneity and invariant moments was computed in order to quantify erosion. Finally, artificial neural networks were constructed and tested as a classifier distinguishing between the normal and the rheumatoid joint. RESULTS: The joint space width of normal was 1.04+/-0.15 mm and the width of patients with rheumatoid arthritis was 0.94+/-0.15 mm. The Homogeneity of normal was 16568.83+/-2669.83 and invariant moments were 6843.45+/-2937.55. They were statistically difference (p<.05). Using these characteristics, artificial neural networks showed that they discriminate between normal and rheumatoid arthritis (AUC=0.91). CONCLUSION: Measuring joint space width, estimating homogeneity, and invariant moments provide the capability to distinguish between a normal joint and a rheumatoid joint.