The significance of VEGF siRNA and bFGF siRNA in invasion and proliferation of pancreatic carcinoma cell / 中华外科杂志

<p><b>OBJECTIVE</b>To investigate the relationship between expression of angiogenic factors and invasion and proliferation of pancreatic cancer cell.</p><p><b>METHODS</b>The three pancreatic cancer cell lines of SW1990, Panc-1 and PCT-3 were divided into four groups respectively: control group, VEGF siRNA group, bFGF siRNA group and VEGF siRNA + bFGF siRNA group. The expression and the secretion of VEGF and bFGF in the three cell lines were inhabited by VEGF siRNA and bFGF siRNA. The proliferation and the invasion of the three cell lines were determined by CCK-8 and Boyden Chamber invasion tests.</p><p><b>RESULTS</b>Expressions of VEGF and bFGF in three cell lines were significantly inhibited by VEGF siRNA and bFGF siRNA. The proliferation was inhabited by VEGF siRNA and bFGF siRNA in SW1990 and Panc-1 (P < 0.05), while was not in PCT-3 (P > 0.05). The invasion was inhabited significantly by VEGF siRNA and bFGF siRNA in the three cell lines (P < 0.05). Combination of VEGF siRNA and bFGF siRNA resulted in more efficient influence in inhibition of invasion in Panc-1 and proliferation in PCT-3 and SW1990 than VEGF siRNA or bFGF siRNA individually.</p><p><b>CONCLUSION</b>The decreased expression of VEGF and bFGF can inhabited the ability of invasion and proliferation of pancreatic cancer cell.</p>

Expression of angiogenic factors in pancreatic carcinoma cell and their significance / 中华外科杂志

<p><b>OBJECTIVE</b>To investigate the relationship between expression of angiogenic factors and invasion and proliferation in pancreatic carcinoma cell.</p><p><b>METHODS</b>Expressions and secretions of angiogenic factors in pancreatic carcinoma cell lines were determined by RT-PCR and ELISA. Proliferation and invasion of pancreatic carcinoma cell lines were determined by CCK-8 and Boyden Chamber invasion tests.</p><p><b>RESULTS</b>Pancreatic carcinoma cell lines showed significantly different ability of invasion and proliferation. Intracellular VEGF expressions of SW1990, Capan-1, Aspc-1, MiaPaCa-2, Panc-1 and PCT-3 were (152.9 +/- 0.6), (224.1 +/- 60.3), (239.2 +/- 2.1), (19.3 +/- 0.7), (165.6 +/- 34.3), and (18.1 +/- 1.4) pg/(10(6)cell.24 h). Extracellular VEGF expressions of SW1990, Capan-1, Aspc-1, MiaPaCa-2, Panc-1 and PCT-3 were (1331.1 +/- 67.8), (3902.6 +/- 79.7), (2657.3 +/- 51.9), (1498.3 +/- 4.8), (4696.8 +/- 45.5), and (1200.5 +/- 42.2) pg/(10(6)cell.24 h). Intracellular bFGF expressions of SW1990, Capan-1, Aspc-1, MiaPaCa-2, Panc-1 and PCT-3 were (66.1 +/- 4.8), (206.8 +/- 99.5), (1532.0 +/- 54.6), (159.2 +/- 11.0), (1612.0 +/- 515.9) and (2781.2 +/- 479.0) pg/(10(6)cell.24 h). Extracellular bFGF expressions of SW1990, Capan-1, Aspc-1, MiaPaCa-2, Panc-1 and PCT-3 were (2.1 +/- 0.6), (10.3 +/- 1.5), (31.0 +/- 0.4), (4.3 +/- 1.2), (43.6 +/- 1.5) and (82.1 +/- 10.4) pg/(10(6)cell.24 h). Intracellular endostatin expressions of SW1990, Capan-1, Aspc-1, MiaPaCa-2, Panc-1 and PCT-3 were (0.2 +/- 0.0), (0.3 +/- 0.0), (4.7 +/- 0.1), (10.8 +/- 0.2), (31.9 +/- 11.7) and (5.4 +/- 0.1) ng/(10(6)cell.24 h). Extracellular endostatin expressions of SW1990, Capan-1, Aspc-1, MiaPaCa-2, Panc-1 and PCT-3 were (0.0 +/- 0.0), (1.6 +/- 0.0), (21.5 +/- 1.1), (40.8 +/- 0.4), (129.2 +/- 1.0) and (20.1 +/- 1.8) ng/(10(6)cell.24 h). Panc-1 enjoying stronger invasion and proliferation showed stronger expressions of VEGF, bFGF and endostatin. Intracellular expressions of bFGF was stronger than extracellular, extracellular expressions of VEGF and endostatin were stronger than intracellular.</p><p><b>CONCLUSION</b>Expressions of angiogenic factors regulated by cancer cell played an important role in progression of pancreatic carcinoma.</p>

The relationship between the changes of proangiogenic factors serum concentrations and progression of pancreatic carcinoma patients / 中华外科杂志

<p><b>OBJECTIVE</b>To investigate the relationship between VEGF, bFGF and IGF-1 serum concentration and progression of pancreatic carcinoma.</p><p><b>METHODS</b>Fifty-six patients with pancreatic carcinoma were divided into resectable group (n = 32) and unresectable group (n = 24). Another group was normal group (n = 20). The expression and significance of these proangiogenic factors were respectively analyzed in different groups.</p><p><b>RESULTS</b>For pancreatic carcinoma group, concentrations of VEGF and bFGF were significantly higher than these of normal group (P < 0.01). Serum VEGF was significantly correlated with the resection of pancreatic carcinoma (P < 0.05) while bFGF and IGF were not. According to univariate analysis, serum VEGF was correlated with tumor grade, nodal disease, vascular invasion, distant metastases and tumor stage. Serum bFGF was associated with tumor size and grade. Serum IGF-1 was correlated with vascular invasion.</p><p><b>CONCLUSIONS</b>Angiogenic factors play important roles in growth, invasion and metastasis. Detection of serum proangiogenic factors may have potential value in diagnosis and prognosis of pancreatic carcinoma.</p>

Relationship between changes of serum concentrations of antiangiogenic factors and disease progression in patients of pancreatic carcinoma / 中华外科杂志

<p><b>OBJECTIVE</b>To investigate the relationship between TSP-1, Angiostatin and Endostatin serum concentrations and progression of pancreatic adenocarcinoma.</p><p><b>METHODS</b>Fifty-six patients with suspected pancreatic cancer were enrolled in the study and divided into resectable group (n = 32) and unresectable group (n = 24) according to evaluation and staging with dual phase helical CT. Histopathologic examinations included postoperative final pathology and preoperative fine needle biopsies. Peripheral blood concentrations of antiangiogenic factors Angiostatin, Endostatin and TSP-1 were detected by using ELISA methods, selecting samples of health people as a control.</p><p><b>RESULTS</b>Serum concentrations of antiangiogenic factors in pancreatic cancer group were significantly higher than those in health group (P < 0.01). Serum concentrations of Endostatin, Angiostatin and TSP-1 were significantly increased in unresectable group, and highly expressed in patients whom tumor sizes were greater than 2 cm and tumor invaded peripancreatic great vessels (P < 0.05). After operation, serum concentrations of Endostatin, Angiostatin and TSP-1 significantly decreased (P < 0.05). There were no significant difference between I, II stage group and III, IV group.</p><p><b>CONCLUSIONS</b>Detection of serum concentrations of antiangiogenic factors may be used to evaluate the resectability of pancreatic cancer and may play important roles in growth, invasion and metastasis of pancreatic cancer.</p>

Diagnosis and management of isolated pancreatic tuberculosis: experience of 13 cases / 中国医学科学杂志(英文版)

<p><b>OBJECTIVE</b>To analyze the diagnosis and treatment of pancreatic tuberculosis.</p><p><b>METHODS</b>Retrospectively reviewed and summarized 13 pancreatic tuberculosis patients' clinical information, presentation, diagnostic methods, therapeutic approaches, and prognosis from 1958 to 2004 at Peking Union Medical College Hospital.</p><p><b>RESULTS</b>All cases presented a wide series of symptoms, including fever in 6 cases, upper abdominal tenderness in 13, epigastric mass in 4, obstructive jaundice in 3, night sweat in 4, weight loss in 7, hypersplenotrophy and hypersplenism in 1, and being complicated with tuberculosis of other organs in 3. One case was diagnosed by clinical symptoms and biopsy of lymph node, and only received anti-tubercular treatment Others were diagnosed by intra-operative biopsy and anti-tubercular treatment, and got well without recurrent tuberculosis in pancreas and other organs during 6 months to 2 years of follow-up. The non-operative case presented extrahepatic portal hypertension.</p><p><b>CONCLUSIONS</b>Pancreatic tuberculosis may be considered in the patients with fever, abdominal tenderness, weight loss, and imaging evidence of regional pancreatic lesion. Efficacy of anti-tubercular agents and laparotomy for pancreatic tuberculosis is evident.</p>