ABSTRACT
OBJECTIVE@#To observe the expression of Bcl-2 and Bax proteins in rat's myocardial cells after Macleaya cordata alkaloids poisoning, and to provide certain molecular biology references for the detection of Macleaya cordata alkaloids poisoning.@*METHODS@#Experimental model of Macleaya cordata alkaloids poisoning was established, the expression levels of Bcl-2 and Bax proteins in these cells were detected by immunohistochemistry, and the results were analyzed by computer image system.@*RESULTS@#The expression levels of Bcl-2 and Bax proteins in myocardial cells in poisoning groups were much greater than those in the control groups (P<0.05).@*CONCLUSION@#If the clinical symptoms may not be obvious, the detection of Bcl-2 and Bax proteins level by immunohistochemistry still could be ancillary method.
Subject(s)
Animals , Female , Male , Rats , Disease Models, Animal , Immunohistochemistry , Myocardium/pathology , Myocytes, Cardiac/pathology , Papaveraceae/chemistry , Papaverine/poisoning , Proto-Oncogene Proteins c-bcl-2/metabolism , Rats, Sprague-Dawley , Staining and Labeling , Time Factors , bcl-2-Associated X Protein/metabolismABSTRACT
OBJECTIVE@#To explore the expression and significance of monocyte chemoattractant protein-1 (MCP-1) in myocardium in sudden death caused by viral myocarditis (VMC).@*METHODS@#To investigate the expression of MCP-1 in VMC sudden death group and control group using improved immunohistochemical technique and to compare the difference of the expression of MCP-1 between two groups with statistical method.@*RESULTS@#In VMC sudden death group, MCP-1 was positively expressed in 17 of 20 cases. While only 4 of the 20 cases in the control group showed a mildly positive expression sparsely in myocardium, and the rest cases were completely negative. The rate of positive expression of MCP-1 in VMC group was obviously higher than that of the control group (P<0.01).@*CONCLUSION@#The expression of MCP-1 detected by immunohistochemistry provides an objective morphologic evidence for the diagnosis of VMC sudden death in forensic pathology.
Subject(s)
Adolescent , Adult , Aged , Child , Child, Preschool , Female , Humans , Male , Middle Aged , Young Adult , Autopsy , Case-Control Studies , Chemokine CCL2/metabolism , Death, Sudden, Cardiac , Forensic Pathology , Immunohistochemistry , Myocarditis/virology , Myocardium/pathology , Staining and LabelingABSTRACT
<p><b>OBJECTIVE</b>To observe the changes of serum free testosterone (FT) and testosterone secreting index (TSI) in ED patients, and to assess the contribution of these two indexes to the diagnosis of ED caused by endocrine factors.</p><p><b>METHODS</b>We studied 120 ED patients and 30 healthy men undergoing pre-marital medical check-up in Jiangsu Province Hospital of TCM by analyzing the scores on erectile function and desire domain in IIEF, testing the serum total testosterone, luteinizing hormone by chemiluminescent enzyme immunoassay (CLIA), measuring free testosterone by radioimmunoassay( RIA), and calculating TSI.</p><p><b>RESULTS</b>Of the 120 ED patients, 5% and 1538% were below the reference norm of TT and FT values respectively. TT, FT and TSI decreased with age, with statistical with FT and TSI, but not with TT. FT and TSI statistically declined with lower IIEF score on ED domain, but this was not the case with TT. There were no significant differences in TI, FT and TSI among different sexual desire groups the ED patients.</p><p><b>CONCLUSION</b>FT is much more valuable than TF in the diagnosis of ED with hypogonadism. Both FT and TSI are important parameters in assessing the severity of ED.</p>
Subject(s)
Adult , Aged , Humans , Male , Middle Aged , China , Erectile Dysfunction , Blood , Metabolism , Immunoenzyme Techniques , Methods , Luteinizing Hormone , Blood , Radioimmunoassay , Surveys and Questionnaires , Testosterone , Blood , Bodily SecretionsABSTRACT
OBJECTIVE@#To study the expression of GFAP and pathologic changes after rats brain concussion, so that to provide evidence on brain concussion for forensic identification.@*METHODS@#Forty-five SD rats were divided into 3, 6, 12, 24 h and 2, 4, 7, 10 d and normal control groups in terms of different wounding time after brain concussion model established, and the expression of GFAP after rats brain concussion were then observed by using SP immunohistochemical method.@*RESULTS@#In normal control brain, low-level GFAP expressions could be observed. After six hours' brain concussion, GFAP positive cells increased obviously. The trend reached to the peak at 7d, partly declined at 10d, then decreased gradually.@*CONCLUSION@#Brain concussion induced the expression of GFAP. The detection of GFAP could be useful for diagnosis of brain concussion on forensic pathology, and could be a reference index for timing of injury after brain concussion.