ABSTRACT
Objective To study the effects of glycosaminoglycan extracted from Pinctada martensii on the proliferation, differentiation and mineralization of newborn rat calvarium osteoblast in vitro. Methods MTT, PNPP and ARS methods were used to measure the cell proliferation, activity of ALP and the function of mineral nodes formation of osteoblasts cultured in vitro. Results Glycosaminoglycan at concentration of 0.008～0.5g?L -1 inhibited mildly the proliferation of osteoblast cells, however, this range of concentrations of glycosaminoglycan markedly increased the ALP activity and stimulated mineral node bone formation in osteoblast. Conclusion Glycosaminoglycan extracted from the Pinctada martensii showed stimulating effects on the differentiation and mineralization of newborn rat calvarium osteoblast in vitro, but the stimulating effect on cells proliferation was not observed.
ABSTRACT
Objective: Diuresis and removing urinary calculus action of Jieshitong tablet were studied. Methods: Metabolic cage method of rats was adopted to be engaged in diuresis experiment; glycol and ammonium chloride had been given orally for 30 days to form concretion model so that a removing urinary calculus trial could be done. Results: High and low doses of Jeshitong tablet both had significant diuresis effect, total urinary output in 3h of drug group increased about 50% than that of control group. Concretio formative rates of Jeshitong tablet group significantly decresed; the stronger removing urinary calculus action, the higher Jieshitong concentraction was. Conclusion: Jieshitong tablet had diuresis and removing urinary calculus action.