ABSTRACT
Proteomic characterization of plasma is critical for the development of novel pharmacodynamic bio-markers.However,the vast dynamic range renders the profiling of proteomes extremely challenging.Here,we synthesized zeolite NaY and developed a simple and rapid method to achieve comprehensive and deep profiling of the plasma proteome using the plasma protein corona formed on zeolite NaY.Specifically,zeolite NaY and plasma were co-incubated to form plasma protein corona on zeolite NaY(NaY-PPC),followed by conventional protein identification using liquid chromatography-tandem mass spectrometry.NaY was able to significantly enhance the detection of low-abundance plasma proteins,minimizing the"masking"effect caused by high-abundance proteins.The relative abundance of middle-and low-abundance proteins increased substantially from 2.54％to 54.41％,and the top 20 high-abundance proteins decreased from 83.63％to 25.77％.Notably,our method can quantify approxi-mately 4000 plasma proteins with sensitivity up to pg/mL,compared to only about 600 proteins iden-tified from untreated plasma samples.A pilot study based on plasma samples from 30 lung adenocarcinoma patients and 15 healthy subjects demonstrated that our method could successfully distinguish between healthy and disease states.In summary,this work provides an advantageous tool for the exploration of plasma proteomics and its translational applications.
ABSTRACT
@#Objective To explore the characteristics of clinical presentations，cerebrospinal fluid (CSF),magnetic resonance imaging (MRI) and prognosis of the anti-GABA-B receptor encephalitis.Methods The clinical data of 30 patients with anti-GABA-B receptor encephalitis were collected.To analysis the characteristics of clinical features，CSF,cranial MRI features and prognosis.Results Among the 30 patients,eighteen were male,and the mean age at presentation were 58 years (range:15~84 years).Seizure was the most common initial symptom.The major clinical features include seizure (26,86.67%),mental and behavioral disorders (26,86.67%),cognitive dysfunction (24,80.00%).CSF cytology test showed that the lymphocytic pleocytosis and the elevated white blood cell counts can be indicative of this disease.Magnetic resonance imaging showed abnormality in the medial temporal lobe and hippocampus in ten patients （35.71%).The disease is closely related to lung cancer (Especially small cell lung cancer,SCLC).During a median follow-up period of 11 months,thirteen patients died.Nine of the patients died with tumors.Conclusions The major clinical features of anti GABA-B encephalitis was seizures.Inflammatory response signals in the medial temporal lobe and hippocampus are image features of this disease.It is closely related to lung cancer (Especially small cell lung cancer,SCLC).Almost half of the patients had poor outcome.