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<p><b>OBJECTIVE</b>To explore the anti-platelet activation effect and partial mechanisms of Taohong Siwu decoction (TSD).</p><p><b>METHOD</b>The effect to venous thrombosis model and pulmonary thromboembolism model induced by vein injecting ADP and Adr was observed. The platelet adhesion rate was analyzed by using spinning glass bottle, and the platelet aggregation rate induced by ADP, Adr was analyzed by using turbidimetry. The acute blood stasis rat model was established to analyze the content of plasm TXB2 and PGI2 by RIA, and the content of VWF, GMP-14 by ELISA.</p><p><b>RESULT</b>TSD could effectively reduce platelet the adhesion rate of normal rat, inhibit the platelet aggregation of normal rat induced by ADP, Adr. It significantly reduced the plasma TXB2 VWF, and GMP-140 level of blood stasis rats. It also had significant tendency to increase 6-keto-PGF1alpha level.</p><p><b>CONCLUSION</b>TSD possessed obvious activity of inhibiting platelet activation. The mechanism related with the restraining of platelet adhesion, platelet aggregation and platelet releasion.</p>
Subject(s)
Animals , Female , Male , Rats , Drugs, Chinese Herbal , Pharmacology , Medicine, Chinese Traditional , Platelet Activation , Platelet AggregationABSTRACT
<p><b>OBJECTIVE</b>To observe the protective effect of serum with Tongqiao Huoxue decoction (TQHXD) on PC12 cells damaged by glutamate(Glu) and provide clinical proof of the formulae.</p><p><b>METHOD</b>Sprague Dawley rats underwent intragastric administration of Tongqiao Huoxue decoction twice a day for five days, the administration dose for rats was 4 g x kg(-1). Thus the serum containing TQHXD was prepared. The model of neurocytes damaged by Glu had been established by adding 12.5 mmol x L(-1) Glu to culture medium of PC12 cells. Photic microscope had been used to observe the morphologic changes of cells, the proliferation and activity of PC12 cells had been detected by MTT. Cell membrane permeability had been investigated by the methods of the coloration of trypan blue and AO/EB, the activities of LDH and the contents of NO in the supernatant of PC12 cells had been detected after 5%, 10%, 20% TQHXD being added to the culture medium of PC12 cells damaged by Glu.</p><p><b>RESULT</b>The morphologic changes in cells were observed under the inverted microscope. Normal PC12 cells in control group were full and bright, but the cells exposed to Glu were shrank; moreover, some cells were disrupted, configuration of the cells in the TQHXD group was closed to that of normal group. Compared with model group, the ratio of living cells in the group being treated by TQHXD after trypan blue staining had a significant increase. After AO/EB staining, cells in TQHXD groups were less being stained by EB. The absorbance (A) values of TQHXD group increased largely. LDH and NO in cell culture supernatant had decreased, it had shown the marked difference.</p><p><b>CONCLUSION</b>TQHXD showed an apparent protective effect on neurocytes damaged by Glu on proliferation activity and membrane permeability.</p>
Subject(s)
Animals , Male , Rats , Cells, Cultured , Drugs, Chinese Herbal , Pharmacology , Glutamic Acid , Toxicity , Neurons , Neuroprotective Agents , Pharmacology , Neurotoxins , Toxicity , PC12 Cells , Rats, Sprague-Dawley , Serum , ChemistryABSTRACT
G-protein coupled receptors(GPCRs)belong to the biggest subfamily of transmembran receptors.Recently,more and more research has been suggested that the dimerization of GPCRs may regulate the physiological and pharmacological activity.With the development of biochemistry technology and molecular biology,a great progress has been achieved in the field of the dimerization of GPCRs.This article will generally demonstrate that the vital roles of the homodimerization and heterdimerization.
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AIM: To investigate the protective effects and mechanisms of Tongqiao Huoxue Decoction on the rats of cerebral ischemia. METHODS: Cerebral ischemia madel was established on the basis of cerebral thrombus and at the same time ligaturing bilateral carotid arteries.These models were used to observe the protective effects of Tongqiao Huoxue Decoction on contents of CGRP、ET、IL-1?,TNF? in rats′ cerebral ischemica. RESULTS: The results showed that Tongqiao Huoxue Decoction decreased the contents of IL-1?、TNF-?、 ET,and increased the contents of CGRP. CONCLUSION: Tongqiao Huoxue Decoction had effects on anti-cerebral ischemia.The protective effects of Tongqiao Huoxue Decoction are related to decreasing the contents of IL-1?,TNF-?,ET,and increasing the contents of CGRP.
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AIM:To study the isolation and identification of curdione from Zedoary oil. METHODS: Silica column was adopted to isolate constituents from Zedoary oil;IR,GC/MS and NMR methods were used to identify its structure. RESULTS: Three constituents were isolated from Zedoary oil,including crystal C_1,C_2 and C_3.Crystal C_1 was identified to be curdione and curdione content in Zedoary oil was 11.31%. CONCLUSION: This method is simple and convenient and it can be used to isolate more quantity of curdione for further pharmaceutical study.
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The anti-inflammatory of waterand fat-soluble alkoloids of Leon-tice Kiagnanensis (WSALK, FSALK ) were described after sc of WSALK (150, 300 mg/kg ) and FSALK (13, 26mg/kg ) . The increased capillary permeability in abdominal cavity and skin of mice induced respectively by 0.7% HAc and histamin were inhibited abviously. WSALK (75, 150, 300mg/kg, ip ) and FSALK (6.5, 13, 26mg/Kg, ip ) markedly inhibited the ear inflammation induced by xylene in mice, carrageenin-iaduces swelling of the ankle in normal on adrenalecto-mized rats, and carrageenin-induced pleurisy in rats. Both compound also inhibited the granuloma induced by cotton pellet after sc of WSALK 300, 600 mg/kg and FSALK 13, 26 mg/kg qd ? 7d.Both compound decreased the content of PGE in exudate formed after injecting carrageenin into the hind paw of rats. These results suggest that both compounds passesses the anti-inflammatory activity and their actions might be relatied to their inhibiting the synthesis or relase of pro-staglandin E. WSALK TI 9.35, FSALK TI 4.50.
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The effects of TSCP and TSCC on aggregation and adhesion of blood platelets were measured by turbidimetric method and salzman method respectively. TSCP and TSCC strongly increase mouse and rat platelet aggregation and platelet adhesion. These effects are important for blood coagulation. cAMP and TXB2 levels in plasma and platelet were determind by RIA TSCP significantly increased the TXB2 level in plasma and platelet. This effect may be the main mechanism of increasing platelet aggregation by TSCP.