Efficiency of castor oil as a storage medium for avulsed teeth in maintaining the viability of periodontal ligament cells

Statement of the Problem: Researchers always seek a new storage medium for avulsed teeth. Castor oil is a vegetable oil with several advantages such as antimicrobial and antioxidant properties, low toxicity, and glutathione preservation capability, low cost, and high availability

Purpose: The purpose of this study was to evaluate and compare the capacity of castor oil as a new storage medium in preserving the viability of periodontal ligament [PDL] cells compared to Hank's balanced salt solution [HBSS] and milk

Materials and Method: Forty freshly extracted human teeth were divided into 3 experimental and 2 control groups. The experimental teeth were stored dry for 30 min and then immersed for 45 min in one of the following media; castor oil, HBSS, and milk. The positive and negative control groups were exposed to 0 min and 2 h of dry time respectively with no immersion in any storage medium. The teeth were then treated with dispase grade II and collagenase and the number of viable PDL cells were counted. Data were analyzed using Kruskal- Wallis test

Results: The percentage of viable cells treated with castor oil, HBSS and milk counted immediately after removal from these media were 46.93, 51.02 and 55.10 % respectively. The statistical analysis revealed that the value for castor oil was significantly lower than HBSS and milk [p> 0.05]

Conclusion: Within the parameters of this study, it appears that castor oil cannot be served as an ideal medium for storage of avulsed tooth. More investigations under in vivo conditions are required to justify the results of this study

Endometrial mesenchymal stem stromal cells in mature and immature sheep: an in vitro study

Background: Endometrial mesenchymal stem stromal cells [EnMSCs] are critical for uterine function, repair, and regeneration

Objective: This study introduced isolation technique of EnMSCs and compared the characteristics of EnMSCs in mature and immature ewes

Materials and Methods: Endometrial tissue samples from the uterus of 10 ewes were collected from the slaughterhouse. Endometrial cells were isolated from tissue using cold incubation and then chopping and treating was performed with collagenase type I. Isolated cells were cultured in cell culture medium and then attached cells to flasks were harvested as EnMSCs and subcultured. To enumerate the cells, the population doubling time [PDT] was determined and 2.2×104 cells in passage 4 were seeded into 24-well culture plates to compare the growth curves of isolated cells. Reverse transcription polymerase chain reaction [RT-PCR] was performed for detection of CD34 and CD73 markers. The osteogenic and adipogenic potential of isolated cells were determined using differentiation tests

Results: EnMSCs adhered to the flasks and displayed spindle-shape. Based on findings of the cell count and the growth curves, the EnMSCs growth was significantly more prominent in immature ewes in comparison to mature sheep. The PDT of EnMSCs in immature ewes was about 21 hr whereas this time period was two times higher [45 hr] in mature sheep. RT-PCR analyses of EnMSCs were positive for CD73 and negative for CD34. EnMSCs were differentiated into osteoblasts and adipocytes

Conclusion: Based on mesenchymal stem cells characters confirmed in EnMSCs, they can be a candidate for cell therapy and regenerative medicine

healing effect of bone marrow-derived stem cells in acute radiation syndrome

Objectives: To determine the effect of bone marrow-derived mesenchymal stem cells [BMSCs] on regeneration of bone marrow and intestinal tissue and survival rate in experimental mice with acute radiation syndrome [ARS]

Methods: Forty mice were randomly divided into two equal groups of A receiving no BMSC transplantation and B receiving BMSCs. BMSCs were isolated from the bone marrow and cultured in DMEM media. Both groups were irradiated with 10 Gy [dose rate 0.28 Gy/ min] 60CO during 35 minutes with a field size of 35×35 for all the body area. Twenty-four hours after gamma irradiation, 150×103 cells of passage 5 in 150 microl medium were injected intravenously into the tail. Animals were euthanized one and two weeks after cell transplantation. They were evaluated histologically for any changes in bone marrow and intestinal tissues. The survival rate in mice were also determined

Results: A significant increase for bone marrow cell count and survival rate were observed in group B in comparison to group A. Histological findings denoted to a healing in sample tissues

Conclusion: BMSCs could significantly reduce the side effects of ARS and increase the survival rate and healing in injured tissue. As such their transplantation may open a window in treatment of patients with ARS

Induction of Asherman's syndrome in rabbit

Background: Uterine synechiae or Asherman's syndrome is a condition that can cause infertility. The present experimental study was designed to establish the rabbit as an animal model for human Asherman's syndrome using the endometrial curettage

Methods: In an experimental study, female adult rabbits [n=18] were randomly divided into intact and ovariectomized groups. One third of caudal part of both uteri was submitted to traumatic endometrial curettage. One group was simultaneously ovariectomized. The intact rabbits were artificially induced ovulation during 10 days after surgery. One third of cranial part of both uteri was selected as the control. Synechiae occurring, luminal area/total area [LA/TA], endometrial area/total area [EA/TA], myometrial and perimetrial area/total area [MPA/TA], endometrial area/uterine wall area [EA/UWA], and myometrial and perimetrial area/uterine wall area [MPA/UWA] ratios of both uteri in six subdivided groups [n=6] were analysed in curetted and intact control parts. On days 15, 30 and 45 following surgery by two-way ANOVA and LSD test [p<0.05]

Results: Histopathologic findings showed significant epithelial damage together with significant inflammatory reaction in the intact curettage group. The LA/TA ratios of the intact curettage group on days 15 and 45 were more than the intact control group on day 15. The EA/TA ratio of the intact curettage group on day 30 was less than the intact control group on day 30

Conclusion: Uterine fibrosis was observed in intact curettage group, and this modified animal model showed a pathogenesis condition similar to intrauterine adhesions observed in human

[ effect of tarragon hydroalcoholic extract on the rate of blood cations and infants sexual ratio in adult mice]

Background and Objectives: tarragon is a plant of Asteraceae Family that has any medical and edible uses. This study aimed to investigate effects of Tarragon hydro-alcoholic extract on serum cations and sexual ratio of mice infants is performed

Materials and Methods: in this experimental study 100 adult male mice and 100 adult female mice divided in 4 groups of males and females for mating to determine infants' sex and to measure males and females cations were used. Each of them were divided into 5 categories of 10 including control, sham, experiment 1, 2 and 3 receiving Tarragon hydro-alcoholic extract at 500,1000 and 1500 mg/kg for 35 days. After phlebotomizing mice' hearts, serum rates of Mg[2+], Ca[2+], K[+]and Na[+] was measured and other females groups with his teammates of male mice as intergroup mated, after the end of period, sex ratio of males and females were measured. The results obtained by using ANOVA and Duncan tests and SPSS 18 software was analyzed

Results: the results showed that tarragon extract in experimental groups caused the significant increase of serum concentrations Mg[2+], Ca[2+] ions in male and female mice and increase females infants' percentage compared to male infants than in control group. Limitations including lack of adequate studies on Tarragon effect on the infants' sex ratio and the pituitary-gonad axis function can be mentioned

Conclusion: the results showed that tarragon extract by having Ca[2+], Mg[2+]and increasing serum ions mentioned and possibly by changing vagina environment pH caused to increase the gynogenesis percentage, so recommended that by supplementary research on tarragon effect on infants' sex in human samples also be considered

Growth Kinetics, characterization, and plasticity of human menstrual blood stem cells

One of the readily available sources of mesenchymal stem cells [MSCs] is menstrual blood-derived stem cells [Men-SCs], which exhibit characteristics similar to other types of MSCs. This study was performed to determine the growth kinetics, plasticity, and characterization of Men-SCs in women. During spring 2014 in the southern Iranian city of Shiraz, menstrual blood [5 mL] was obtained from 10 women on their third day of menstruation in 2 age groups of 30 to 40 and 40 to 50 years old. Ficoll was used to separate the mononuclear cell fraction. After the Men-SCs were cultured, they were subcultured up to passage 4. Growth behavior and population doubling time were evaluated by seeding 5×10[4] cells into 12- and 24-well culture plates, and the colonies were enumerated. The expression of CD44, CD90, and CD34 was evaluated. The osteogenic potential was assessed by alizarin red staining. The Men-SCs were shown to be plastic adherent and spindle-shaped. Regarding the growth curves in the 12-24-well culture plates, it was demonstrated that in the women aged between 30 and 40 years, population doubling time was 55.5 and 62 hours, respectively, while these values in the women aged between 40 and 50 years were 70.4 and 72.4 hours, correspondingly. Positive expression of CD44 and CD90 and negative expression of CD34 were noted. In the osteogenic differentiation medium, the cells differentiated toward osteoblasts. As human Men-SCs are easily collectable without any invasive procedure and are a safe and rapid source of MSCs, they can be a good candidate for stem cell banking and cell transplantation in women

Isolation, Characterization and Growth Kinetic Comparison of Bone Marrow and Adipose Tissue Mesenchymal Stem Cells of Guinea Pig

BACKGROUND: Mesenchymal stem cells (MSCs) from different sources have different characteristics. Moreover, MSCs are not isolated and characterized in Guinea pig for animal model of cell therapy. AIM OF THE WORK: was the isolating of bone marrow MSCs (BM-MSCs) and adipose tissue MSCs (AT-MSCs) from Guinea pig and assessing their characteristics. MATERIAL AND METHODS: In this study, bone marrow and adipose tissue were collected from three Guinea pigs and cultured and expanded through eight passages. BM-MSCs and AT-MSCs at passages 2, 5 and 8 were seeded in 24-well plates in triplicate. Cells were counted from each well 1~7 days after seeding to determine population doubling time (PDT) and cell growth curves. Cells of passage 3 were cultured in osteogenic and adipogenic differentiation media. RESULTS: BM-MSCs and AT-MSCs attached to the culture flask and displayed spindle-shaped morphology. Proliferation rate of AT-MSCs in the analyzed passages was more than BM-MSCs. The increase in the PDT of MSCs occurs with the increase in the number of passages. Moreover, after culture of BM-MSCs and AT-MSCs in differentiation media, the cells differentiated toward osteoblasts and adipocytes as verified by Alizarin Red staining and Oil Red O staining, respectively. CONCLUSION: BM-MSCs and AT-MSCs of Guinea pig could be valuable source of multipotent stem cells for use in experimental and preclinical studies in animal models.

effect of blood loss in the presence and absence of severe soft tissue injury on hemodynamic and metabolic parameters; an experimental study

The effect of severe soft tissue injury on the severity of hemorrhagic shock is still unknown. Therefore, the present study was aimed to determine hemodynamic and metabolic changes in traumatic/hemorrhagic shock in an animal model. Forty male rats were randomly divided into 4 equal groups including sham, hemorrhagic shock, soft tissue injury, and hemorrhagic shock + soft tissue injury groups. The changes in blood pressure, central venous pressure [CVP] level, acidity [pH], and base excess were dynamically monitored and com-paredsented. Mean arterial blood pressure decreased significantly in hemorrhagic shock [df: 12; F=10.9; p<0.001] and severe soft tissue injury + hemorrhagic shock [df: 12; F=11.7; p<0.001] groups 15 minutes and 5 minutes after injury, respectively. A similar trend was observed in CVP in severe soft tissue injury + hemorrhagic shock group [df: 12; F=8.9; p<0.001]. After 40 minutes, pH was significantly lower in hemorrhagic shock [df: 12; F=6.8; p=0.009] and severe soft tissue injury + hemorrhagic shock [df: 12; F=7.9; p=0.003] groups. Base excess changes during follow ups have a similar trend. [df: 12; F=11.3; p<0.001]. The results of this study have shown that the effect of hemorrhage on the decrease of mean arterial blood pressure, CVP, pH, and base excess is the same in the presence or absence of soft tissue injury

[The effect of palm pollen extract on sexual hormones and the numbers of spermatozoa dynastic cell in adult male mice]

Background and Objectives: infertility in men is mainly caused by as low or abnormal spermatogenesis and hormonal problems. Because of high costs and potential side effect of routine therapeutic modalities. It seems that use of herbal medicine have fewer side effects; the aim of the study is to examine the effect of palm pollen extract on serum levels of hormones and spermatozoa dynastic cells in adult male mice

Materials and Methods: this experimental study was conducted on 40 adult male rats. They were divided into 5 groups 8, in each group that included controls [no treatment], only saline treated and three experimental groups that received aqueous extract of date palm pollen in three different dosages, doses 100, 200 and 400 mg / kg respectively. All prescriptions were done intraperitoneally for 35 days. At the end of experiment animals were phlebotomized from the heart and by separation of serum samples, amounts of testosterone, estrogen, progesterone were counted, and by separation of testicle, the numbers of sperrnatogonia, sperrnatocytes and spermatid were counted and the results were analyzed using unilateral ANOV A and Duncan methods

Results: the results showed that aqueous extracts of palm pollen dose-dependently caused significant increasing the levels of estrogen, progesterone and testosterone and increasing the numbers of spermatocytes and spennatid

Conclusions: the presence of compounds such as cadmium, zinc, steroids, flavonoids, saponins, lipids, and by substances that inhibiting the enzyme 5- alpha redoctase could increase testosterone and sexual dynastic cells

prevalence of dyspepsia symptoms and its correlation with the quality of life among Qashqai Turkish migrating nomads in Fars Province, Southern Iran

To determine the prevalence of dyspepsia and its correlation with quality of life in Fars Qashqai Turkish migrating nomads from Southern Iran. During 2010 we enrolled 397 Qashqai migrating nomads from Southern Iran who were 25 years of age or older. Participants completed a questionnaire that consisted of demographic factors, lifestyle data, gastrointestinal symptoms, and the Short-Form 36 Health Survey [SF-36] questionnaire. There was a 48% prevalence of dyspepsia symptoms among participants. The prevalence was higher among females, those less than 35 years of age, married participants, and those with a low body mass index [BMI]. The correlation between dyspepsia and quality of life was significant. Dyspeptic patients were classified into ulcer-like [27.9%], dysmotility-like [26.2%], and unspecified [45.9%] groups. A significant correlation existed between dyspepsia symptoms and consumption of dairy products, drinking water and tea before and after meals, smoking, dysphagia, reflux, heartburn, and use of non-steroid anti-inflammatory drugs and acetaminophen. The high prevalence of dyspepsia in Qashqai nomads necessitates educational health programs for the migrating tribes in order to decrease prevalence of this disease

Induction of Spermatogenesis by Bone Marrow-derived Mesenchymal Stem Cells in Busulfan-induced Azoospermia in Hamster

BACKGROUND: Bone marrow-derived mesenchymal stem cells (BM-MSCs) have potential of differentiation and they secrete anti-inflammatory cytokines and growth factors which make them appropriate for cell therapy. AIM OF THE WORK: Were to evaluate the healing effect of BM-MSCs transplantation on germinal cells of busulfan-induced azoospermic hamsters. MATERIAL AND METHODS: In the present experimental case control study, BM-MSCs were isolated from bone marrow of donor albino hamsters. Five mature male recipient hamsters received two doses of 10 mg/kg of busulfan with 21 days interval to stop endogenous spermatogenesis. After induction of azoospermia, right testis of hamsters was injected with 106 BM-MSCs via efferent duct and the left one remained as azoospermia control testis. Five normal mature hamsters were selected as normal intact control. After 35 days, testes and epididymis of three groups were removed for histological evaluation. RESULTS: Histomorphological analyses of BM-MSCs treated testes and epididymis showed the epithelial tissue of seminiferous tubules had normal morphology and spermatozoa were present in epididymis tubes. Spermatogenesis was observed in most cell-treated seminiferous tubules. The untreated seminiferous tubules were empty. CONCLUSION: Transplanted BM-MSCs could successfully induce spermatogenesis in seminiferous tubules of azoospermic hamster. Therefore, BM-MSCs can be an attractive candidate in cell transplantation of azoospermia.

In vitro and in vivo potential of RH strain of toxoplasma gondii [type I] in tissue cyst forming

Based on recent studies, there are controversial reports on the capacity of tissue cyst forming of Toxoplasma gondii RH strain. In this study, the capacity was evaluated by in vivo and in vitro experiments. RH strain was subcutaneously inoculated to ten Wistar rats. After one month, their blood, brain, tongue and diaphragm were collected and evaluated by MAT, PCR, pathological and bioassay methods. The parasite was cultivated in the cell monolayer. To change to bradyzoite, the media pH was altered to 6.8. Biological aspect of the bradyzoites was evaluated by incubation in acidic pepsin and it's inoculation in ten BALB/c mice. All rats showed antibodies to Toxoplasma at titers >/= 1:320 but no DNA and tissue cyst were detected in the tissues. Following intraperitoneal inoculation of rats' brain homogenate into BALB/c mice, no infection was established in none of the animals. During presence of cell culture, in acid media for a 3-5 days period, cyst-like structures were noticed when they were stained with PAS. The visible bradyzoites in the cysts that were incubated in acid pepsin medium were not able to kill any mice. This study confirmed that Iranian RH strain has lost the potential of tissue cyst forming in rats and bradyzoites cultivated in cell culture lost their resistance to acidic condition, so this strain can be a candidate for future vaccine researches

healing effect of teucrium polium in acetic acid-induced ulcerative colitis in the dog as an animal model

Inflammatory bowel diseases [IBD], which include ulcer-ative colitis [UC] and Crohn's disease [CD], are debilitating and chronic disorders with unpredictable courses and complicated treatment measures. Therefore, an efficient treatment protocol seems necessary as therapeutic prophylaxis for these disorders. This study aims to determine the healing effect of Teucrium polium [T. polium] in acetic acid-induced UC in an experimental dog model. From September to December 2010, eight male [20-25 kg] crossbred dogs were used for induction of UC by 6% acetic acid, tran-srectally. After one week, three biopsies [10, 20 and 30 cm proximal to the anal verge] were taken from the colon of each animal for histological studies. In the presence of UC, 400 mg/kg/day of T. polium extract was administered orally and transrectally [via enema] for 30 days in six of the dogs. The remaining two dogs were used as controls and did not receive T. polium. Multiple biopsies were taken 7, 14, and 30 days after discontinuation of T. polium in the same manner as before treatment. After administration of acetic acid, we noted the presence of multiple ulcers, diffuse inflammation, PMN infiltration in the lamina propria, glandular destruction and goblet cell depletion. Treatment with T. polium restored the colonic architecture with an increased number of healthy cells and a reduction in inflammatory cells. Damage of the surface epithelial cells and mucosal layer of the lumen were reversed, which lead to faster ulcer healing. T. polium may be a treatment choice for UC and can broaden the current therapy options for UC

Migraine risk factors in Qashqai migrating nomads: an adjusted multiple correspondence analysis approach

To determine the migraine risk factors in Qashqai migrating nomads using an adjusted multiple correspondence analysis approach. In summer 2006, 750 Qashqai migrating nomads aged 25 years or older in Fars Province, Southern Iran were enrolled using a multiple-stage cluster random sampling. A questionnaire consisting of demographic characteristics, lifestyle and migraine diagnosis was used for each subject. The association of each risk factor was assessed using an adjusted multiple correspondence analysis. The prevalence of migraine was 18% in males and 28% in females. It was higher in overweight and obese subjects, those who consumed roasted foods, widows, female gender and in the age group of 35-50 years old. Multiple correspondence analyses were suitable choices to determine detailed results of complex categorical data in migraine risk factors

Graft survival rate in pediatric renal transplantation: a single center experience

Renal transplantation is the best option for treatment of the end-stage renal diseases and has more advantages than dialysis. The objective of this study was to determine the ten-year graft survival rate of renal transplantation and its contributing factors among a group of Iranian patients aged 18 years or younger. In a retrospective cohort we aimed to study the ten-year graft survival rate of renal transplantation and its contributing factors among pediatric patients who have been transplanted from March 1999 to March 2009 in Shiraz Namazi Hospital Transplantation Center, southern Iran. Kaplan-Meier method was used to determine the survival rate, log rank test to compare survival curves, and Cox regression model to determine hazard ratios and to model variables affecting survival. The 1, 3, 5, 7 and 10 year-graft survival rates were 96.8, 88.8, 82.8, 78.1, 78.1 percent, respectively. Cox regression model revealed that the duration of hospitalization was one of the important factors in graft survival rate in renal transplantation. In this study, the 10 year-graft survival rate for pediatric renal transplantation was 78.1%. Graft survival was significantly related to hospital stay after the operation. Donor source, cold ischemia time, immunosuppressive drugs regimen, time to diuresis, and cause of ESRD did not influence the survival rate.

Incidence of breast cancer in Fars province, southern Iran: a hospital-based study

Breast cancer is still considered as one of the most common female cancers worldwide regardless of the countries' level of development. This study determines the incidence of breast cancer in Fars Province, Southern Iran. This study used patients' records from Shiraz University of Medical Sciences Cancer Registry Centre, which is a Hospital- Based Registry of Nemazee Hospital. Data were recorded based on International Classification of Diseases for Oncology [ICD-O] and compromised all invasive cancers in ICD-10 categories of C- 00 to C-80. The findings were shown as the number of cases by site [ICD-10] and gender, with crude incidence [CRs], agespecific incidence and age-standardized incidence rates [ASRs] per 100,000 persons per year, performed by direct method using the world standard population. The age group of 40-49 years had the highest rate of breast cancer and naturally most cases were post-menopause ones. Most cases were diagnosed in moderate differentiated state with an increasing trend. Early diagnosis of in situ neoplasms has not increased over time in comprised with malignant cases. The number of diagnosed cases has sharply increased after year 2004 especially during post-menopause period. As the number of diagnosed cases has increased during postmenopausal period, screening and health programs seem necessary for menopause women

Correlation of quality of life with gastroesophageal reflux disease amongst Qashqai nomads in Iran

Gastoesophageal reflux disease [GERD] is one of the most common gastrointestinal diseases encountered in today's clinic practice. GERD symptoms are troublesome and disruptive to the physical, social and emotional well-being of many patients. This is a cross-sectional study performed on Qashqai nomads or Fars Province in southern Ian to determine the impact of GERD on quality of life. We randomly selected 748 subjects over the age of25 years. Subjects completed two questionnaires conducted by interviews. The first one consisted of questions on gastroesophageal reflux symptoms. The second questionnaire was the Short Form Health Survey [SF-36], a generic health-related quality of life instrument that consists of 36 items divided into eight dimensions. It has a 0-to 100 point scale where higher scores show better functioning and well-being. Of 748 Qashqai migrating nomads who participated in the study, 717 subjects [mena age: 43 +/- 14.2 years] completed the GERD questionnaire and only 372 subjects completed the SF-36 questionnaire due to their busy lifestyles, and GERD was reported in 106 subjects [28.5%]. For all dimensions of SF-36, the mean score was consistently lower in patients with GERD was reported in 106 subjects [28.5%]. For all dimensions of SF-36, the mean score was consistently lower in patients with GERD compared to non-GERD subjects [P<0.001]. The dimension most frequently impaired was role-physical [40.9 vs. 77.3] followed by role-emotional [44.7 vs. 77.5], physical functioning [66.9 vs. 84.6], and general health [46.8 vs. 63.8]. An association existed between impairment in quality of life and frequency [P<0.05], but not severity, of GERD symptoms

Leishmania major in Tatera indica in Estahban, Southern Iran: microscopy, culture, isoenzyme and PCR

This study identifies the infected rodent hosts to Leishmania major in Estahban town, southern Iran during 2004-2005. The rodents were caught alive from April 2004 to April 2005 in Estahban town, south of Iran and examined for any skin lesion. An impression was provided from the tissues of feet, tail, ears and any patent lesion, stained with Giemsa and studied microscopically for the presence of amastigotes. All samplings were cultured at 25°C in rabbit blood agar and considered negative if no promastigotes were visible during a two months period. The parasites from any positive culture were cryopreserved in liquid nitrogen pending their identification in PCR and isoenzyme electrophoresis. The femoral bones were histologically and ultrastrucrurally studied. Among 13 captured rodents, 8 were Tatera indica [5 male and 3 female Indian gerbils] and 5 were Rattus rattus [3 males and 2 females]. Just one female T. indica was smear-positive for amastigotes in Mohmmad Abad village. This rodent was also found culture positive for leishmanial infection which was confirmed by PCR and enzyme electrophoresis. At histological and ultrastructural levels, many clusters of amastigotes were noticed in the foamy macrophages of the femoral bone bone marrow. T. indica was found for the first time in the area and can be one of the rodents to be a potential reservoir host of L. major. It was also shown that femoral bone marrow was the tissue of choice to confirm the presence of macrophages containing the amastigote form of the parasite