1.
Indian J Exp Biol
; 1994 Sep; 32(9): 672-3
Article
in English
| IMSEAR
| ID: sea-59505
ABSTRACT
6-Amino penicillanic acid (6-APA) was condensed with D-alpha-phenyl glycine chloride in presence of E. coli NCIM 2563 to form ampicillin. At pH 5, 30% of 6-APA was converted to ampicillin during 1 hr incubation. When E. coli cells were immobilized in calcium alginate beads, the activity remained unaffected even after six batches of bioconversion. Inclusion of glutaraldehyde as multifunctional cross-linking agent, improved the stability of the biocatalyst beads.
Subject(s)
Alginates , Ampicillin/metabolism , Cross-Linking Reagents , Escherichia coli/metabolism , Glucuronic Acid , Hexuronic Acids , Microspheres
2.
Article
in English
| IMSEAR
| ID: sea-64129
3.
Article
in English
| IMSEAR
| ID: sea-64078