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1.
Anatomy & Cell Biology ; : 242-249, 2019.
Article in English | WPRIM | ID: wpr-762240

ABSTRACT

The aim of this study was to identify the three-dimensional topography of the sphenoid door jamb (SDJ) in the lateral orbital wall and to propose navigational guidelines for safe deep lateral decompression using surgical landmarks. The 120 orbits and SDJs of 60 subjects were three-dimensionally reconstructed using Mimics software. The mean volumes of the orbit and SDJ were 24.3 mm³ and 2.0 mm³, respectively. The mean distances from the lateral orbital margin (LOM) to the anterior and posterior margins of the SDJ were 13.2 and 36.3 mm, respectively. The mean distances from the superior orbital fissure to the LOM and to the posterior margin of the SDJ were 40.2 mm and 4.6 mm, respectively. The mean distances from the inferior orbital fissure (IOF) to the anterior and posterior margins of the SDJ were 3.8 mm and 20.5 mm, respectively. In the superior approach of the orbit, it can be predicted that the area up to 3 cm posterior from the LOM is safe, while 1 cm posterior from the safe zone could be a dangerous zone. In the inferior approach of the orbit, the safe area will be about 1 cm posterior from the anterior tip of the IOF, and the area up to 1 cm posterior from the safe zone should be approached with extreme care.


Subject(s)
Decompression , Graves Disease , Orbit
2.
Clinical and Experimental Reproductive Medicine ; : 207-213, 2017.
Article in English | WPRIM | ID: wpr-226343

ABSTRACT

OBJECTIVE: This study investigated the prevalence of infections with human papillomavirus, Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma hominis, and Mycoplasma genitalium in the semen of Korean infertile couples and their associations with sperm quality. METHODS: Semen specimens were collected from 400 men who underwent a fertility evaluation. Infection with above five pathogens was assessed in each specimen. Sperm quality was compared in the pathogen-infected group and the non-infected group. RESULTS: The infection rates of human papillomavirus, C. trachomatis, U. urealyticum, M. hominis, and M. genitalium in the study subjects were 1.57%, 0.79%, 16.80%, 4.46%, and 1.31%, respectively. The rate of morphological normality in the U. urealyticum-infected group was significantly lower than in those not infected with U. urealyticum. In a subgroup analysis of normozoospermic samples, the semen volume and the total sperm count in the pathogen-infected group were significantly lower than in the non-infected group. CONCLUSION: Our results suggest that infection with U. urealyticum alone and any of the five sexually transmitted infections are likely to affect sperm morphology and semen volume, respectively.


Subject(s)
Humans , Male , Chlamydia trachomatis , Family Characteristics , Fertility , Mycoplasma genitalium , Mycoplasma hominis , Prevalence , Semen , Semen Analysis , Sexually Transmitted Diseases , Sperm Count , Spermatozoa , Ureaplasma urealyticum
3.
Nutrition Research and Practice ; : 43-50, 2017.
Article in English | WPRIM | ID: wpr-202899

ABSTRACT

BACKGROUND/OBJECTIVES: The present study investigated the hypothesis that a highly pure linear β-1,3-glucan produced by Agrobacterium sp. R259 enhances human natural killer (NK) cell activity and suppresses pro-inflammatory cytokines. SUBJECTS/METHODS: In an eight-week, double-blind, randomized, placebo-controlled clinical trial, 83 healthy adults with white blood cell counts of 4,000-8,000 cells/µL were participated and randomly assigned to take two capsules per day containing either 350 mg β-1,3-glucan or placebo. Six participants withdrew their study consent or were excluded due to NK cell activity levels outside the normal range. NK cell activity and serum levels of immunoglobulin G (IgG) and cytokines, such as interferon (IFN)-γ, interleukin (IL)-2, IL-4, IL-6, IL-10, IL-12 and tumor necrosis factor (TNF)-α were measured. RESULTS: NK cell activity and the serum levels of IL-10 were significantly higher from baseline to week 8 in the β-glucan group compared with the placebo group (P = 0.048, P = 0.029). Consumption of β-1,3-glucan also significantly increased NK cell activity compared with placebo after adjusting for smoking and stress status (P = 0.009). In particular, the effect of β-1,3-glucan on NK cell activity was greater in participants with severe stress than in those experiencing mild stress. However, the administration β-1,3-glucan did not significantly modulate the levels of IFN-γ, IL-2, IL-4, IL-6, IL-12, TNF-α and IgG compared with the placebo. CONCLUSION: The results showed that supplementation with bacterial β-1,3-glucan significantly increased NK cell activity without causing any adverse effects. Additionally, the beneficial effect of β-1,3-glucan on NK cell activity was greater in participants experiencing severe stress.


Subject(s)
Adult , Humans , Agrobacterium , Capsules , Cytokines , Glucans , Immunoglobulin G , Interferons , Interleukin-10 , Interleukin-12 , Interleukin-2 , Interleukin-4 , Interleukin-6 , Interleukins , Killer Cells, Natural , Leukocyte Count , Reference Values , Smoke , Smoking , Tumor Necrosis Factor-alpha
4.
Nutrition Research and Practice ; : 525-525, 2017.
Article in English | WPRIM | ID: wpr-55958

ABSTRACT

The paper by Lee YJ et al. [1] was printed with spelling errors.

5.
Immune Network ; : 161-166, 2015.
Article in English | WPRIM | ID: wpr-148260

ABSTRACT

Early growth response (Egr)-1 is a Cys2-His2-type zincfinger transcription factor. It has been shown to induce survival and proliferation of immature and mature B cells, respectively, but its role in the differentiation of B cells into plasma cells remains unclear. To examine the effects of Egr-1 deficiency on the activation of B cells, naive B cells from Egr1-/- mice and their wild-type (WT) littermates were activated to proliferate and differentiate, and then assayed by FACS. Proportions of cells undergoing proliferation and apoptosis did not differ between Egr1-/- and WT mice. However, Egr1-/- B cells gave rise to fewer plasma cells than WT B cells. Consistently, Egr1-/- mice produced significantly lower titer of antigen-specific IgG than their WT littermates upon immunization. Our results demonstrate that Egr-1 participates in the differentiation program of B cells into plasma cells, while it is dispensable for the proliferation and survival of mature B cells.


Subject(s)
Animals , Mice , Apoptosis , B-Lymphocytes , Immunization , Immunoglobulin G , Plasma Cells , Transcription Factors
6.
Nutrition Research and Practice ; : 185-191, 2013.
Article in English | WPRIM | ID: wpr-156145

ABSTRACT

In previous studies, we found that the consumption of legumes decreased bone turnover in ovariectomized rats. The purpose of the present study is to determine whether the protective effects on bone mineral density (BMD) and the microarchitecture of a diet containing legumes are comparable. In addition, we aim to determine their protective actions in bones by studying bone specific gene expression. Forty-two Sprague-Dawley rats are being divided into six groups during the 12 week study: 1) rats that underwent sham operations (Sham), 2) ovariectomized rats fed an AIN-93M diet (OVX), 3) ovariectomized rats fed an AIN-93M diet with soybeans (OVX-S), 4) ovariectomized rats fed an AIN-93M diet with mung beans (OVX-M), 5) ovariectomized rats fed an AIN-93M diet with cowpeas (OVX-C), and 6) ovariectomized rats fed an AIN-93M diet with azuki beans (OVX-A). Consumption of legumes significantly increased BMD of the spine and femur and bone volume of the femur compared to the OVX. Serum calcium and phosphate ratio, osteocalcin, expression of osteoprotegerin (OPG), and the receptor activator of nuclear factor kappaB ligand (RANKL) ratio increased significantly, while urinary excretion of calcium and deoxypyridinoline and expression of TNF-alpha and IL-6 were significantly reduced in OVX rats fed legumes, compared to OVX rats that were not fed legumes. This study demonstrates that consumption of legumes has a beneficial effect on bone through modulation of OPG and RANKL expression in ovariectomized rats and that legume consumption can help compensate for an estrogen-deficiency by preventing bone loss induced by ovarian hormone deficiency.


Subject(s)
Animals , Rats , Amino Acids , Bone Density , Calcium , Cytokines , Diet , Fabaceae , Femur , Gene Expression , Interleukin-6 , Osteocalcin , Osteoprotegerin , RANK Ligand , Rats, Sprague-Dawley , Salicylamides , Glycine max , Spine , Tumor Necrosis Factor-alpha
7.
Korean Journal of Physical Anthropology ; : 153-166, 2012.
Article in Korean | WPRIM | ID: wpr-59331

ABSTRACT

Interest in the correction of jaw deformities has increased due to the development of techniques and instruments of jaw operation. The accurate position of the mandibular foramen and lingula is important for the prevention of the complications and successful inferior alveolar nerve block. This study was conducted on 104 dried mandibles (65 males, 39 females) from Korean cadaver. The positions of the mandibular foramen and lingula were measured on the coronoid notch, occlusal plane, mandibular notch, gonion, koronoid and kondylion. The structures of the mandibular foramen and lingula were determined and the thickness of ramus and the angle of mandible were measured. Independent t-tests and paired t-tests were performed to determine the statistically significant difference. The results as follows; The line between coronoid notch and posterior border of ramus was located on the same level with the tip of lingula in 42.2% of the cases, and in between the mandibular foramen and the tip of lingula in 33.8% of the cases. The occlusal plane was located on the level between the mandibular foramen and the tip of lingula in 47.7% of cases, and on the same level with the tip of lingula in 35.6% of the cases. The line between lower end of the mandibular notch to inferior border of the mandible, in parallel to the posterior border of the mandibles, met the tip of lingula in 55.8% of the cases. The mandibular foramen was located posteriorly to the midpoint on the anteroposterior (AP) width of the ramus. It was located at 57.3% of AP width from the coronoid notch and 56.5% from the occlusal plane. The mandibular foramen and the tip of lingula were located superiorly to the midpoint on the vertical height of the ramus, on the 48.5% and 35.7% of vertical distance from the coronoid notch, respectively. The mandibular foramen was located at 40% of the length on the line from the gonion to the koronion. The results of this study will provide important morphometric information to prevent the complications in mandibular surgery and also provide the basis for the development of the new techniques.


Subject(s)
Humans , Male , Cadaver , Congenital Abnormalities , Dental Occlusion , Jaw , Mandible , Mandibular Nerve
8.
Korean Journal of Physical Anthropology ; : 167-175, 2012.
Article in Korean | WPRIM | ID: wpr-59330

ABSTRACT

Ischemic preconditioning (IP), short pre-treatment sublethal ischemia, induces a state of protection against subsequent prolonged ischemia-reperfusion. The purpose of this study was to investigate the expression of HO-1, HSP70, and iNOS proteins in the liver subjected to the courses of reperfusion after repetitive cycles of remote IP in the rat. Using thirty five week-old rats, the remote preconditioning was undertaken by vascular clamp occlusion of blood flow to one hindlimb, with 3 and 10 cycles of 5 minutes occlusion followed by 5 minutes reperfusion. The liver was removed 0, 3, 6, 24, and 72 hours of reperfusion after remote IP and assayed by immunohistochemical staining and Western blotting analyses for anti-HO-1, anti-HSP70, and anti-iNOS antibodies. The expression of HO-1 in rat liver increased at 72 hours of reperfusion groups after 3 and 10 cycles of remote IP, compared with normal control groups. The expression of HSP70 in rat liver increased at 6 hours of reperfusion groups after 3 cycles of remote IP, compared with normal control groups. The expression of HSP70 in rat liver increased at 0 hour of reperfusion groups after 10 cycles of remote IP, compared with normal control groups and decreased at 24 and 72 hours of reperfusion groups. The expression of iNOS in rat liver increased at 24 hours of reperfusion groups, but decreased at 72 hours of reperfusion groups after 3 and 10 cycles of remote IP, compared with normal control groups. In summary, these results showed that at early phase of reperfusion after remote IP, HSP70 expression was increased in rat liver. However, at 72 hrs of reperfusion after remote IP, HO-1 expression was increased and iNOS expression was decreased in rat liver.


Subject(s)
Animals , Rats , Blotting, Western , Hindlimb , Ischemia , Ischemic Preconditioning , Liver , Proteins , Reperfusion
9.
Korean Journal of Physical Anthropology ; : 1-10, 2012.
Article in Korean | WPRIM | ID: wpr-144002

ABSTRACT

This study was undertaken to investigate the morphological changes of scalp hair according to weathering after burial and to offer basic data for forensic science. To examine the effect of weathering degree, the 51 human scalp hairs buried after death were taken from deceased individuals in tombs in Korea. The morphological changes in weathering hair shafts of the scalp were investigated using the transmission electron microscopes (TEM). There were little morphological differences of weathering depending on the burial period. The weathering changes includes detachment of cuticle layer, aggregation of macrofibril and hollow formation in the cortex according to the burial period. These results could provide basic data to the field of forensic medicine.


Subject(s)
Humans , Burial , Electrons , Forensic Medicine , Forensic Sciences , Hair , Korea , Scalp , Weather
10.
Korean Journal of Physical Anthropology ; : 1-10, 2012.
Article in Korean | WPRIM | ID: wpr-143995

ABSTRACT

This study was undertaken to investigate the morphological changes of scalp hair according to weathering after burial and to offer basic data for forensic science. To examine the effect of weathering degree, the 51 human scalp hairs buried after death were taken from deceased individuals in tombs in Korea. The morphological changes in weathering hair shafts of the scalp were investigated using the transmission electron microscopes (TEM). There were little morphological differences of weathering depending on the burial period. The weathering changes includes detachment of cuticle layer, aggregation of macrofibril and hollow formation in the cortex according to the burial period. These results could provide basic data to the field of forensic medicine.


Subject(s)
Humans , Burial , Electrons , Forensic Medicine , Forensic Sciences , Hair , Korea , Scalp , Weather
11.
Korean Journal of Physical Anthropology ; : 67-75, 2010.
Article in Korean | WPRIM | ID: wpr-6956

ABSTRACT

In recent times the modification and injury of foot are shown due to various activity. It is essential to have detailed knowledges about the anatomical structure of medial longitudinal arch of foot for doing orthodigita or making shoes to prevent variation of medial longitudinal arch of foot. This study aimed to measure the constitution and to suggest the index of medial longitudinal arch of foot. Fifty four feet (30 M/24 F) obtained from the collection of the Department of Anatomy and Cell Biology of College of Medicine, Hanyang University were studied. We measured the length of foot, the height of foot, and the first ray angle. Also, we found the index of medial longitudinal arch and compared this results with various races anthropologically. Each items was analyzed using SPSS win 13.0. The length of foot was 211.12+/-13.02 mm and the height of foot was 39.03+/-6.27 mm. There was statistically significant difference between the values of male and female and among the age groups (p>0.001). The first ray angle was 24.98+/-2.16 degrees. There was statistically significant difference between the values of male and female (p>0.001). The medial longitudinal arch index was 18.43+/-2.33. The date shows clear distinction between the value of index in this study and the American's value of index as a result of comparing races. The results of this study would be useful to clarify the characteristics of the medial longitudinal arch.


Subject(s)
Female , Humans , Male , Constitution and Bylaws , Racial Groups , Foot , Shoes
12.
Korean Journal of Physical Anthropology ; : 77-86, 2010.
Article in Korean | WPRIM | ID: wpr-6955

ABSTRACT

The morphometric data which reflect the quality and quantity of the mandible are very important to the surgeon for the mandible. It is very useful clinically to predict the status of the mandible indirectly by other medical examinations. This study was undertaken to clarify the correlations of the mandible and dentition to predict the thickness and bone mineral density of the mandible. Sixty-one mandibles (32 M/29 F, mean age: 66.72 years) obtained from the collection of the Department of Anatomy and Cell Biology of Hanyang Medical College were analyzed. The bone mineral density was measured by dual energy X-ray absorptiometry. In the mandible, bone mineral densities at the mandiblular angle, between molars and premolars, around incisors and the thickness, and the length at the sections through the 1st and the 2nd molars were measured. The data were analysed with SPSS 12.0 program (One-Way ANOVA) according to age, gender and dentition, to verify the statistical significance and the correlation between the thickness and bone mineral densities. The obtained results were as follows. 1. The bone mineral density in the mandibular areas were variable, but statistically insignificant except incisor area. The bone density of the mandible was highly correlated with the thickness of cortical bones and the highest correlation coefficient was shown in sum of the thickness of buccal and lingual cortical bone (correlation coefficient, r=0.622) 2. The thickness of cortical bones at the sections through the molars in the man were greater than those in the woman. The correlation coefficient between the thickness and bone mineral density at the molars were greater in the woman. 3. The bone mineral density of whole mandible, the thickness of lingual and basal cortical bones and the height of alveolar ridge were significantly higher in the specimens with both of the 1st and 2nd mandibular molar teeth. The results of this study represents the significant differences of bone mineral density in the mandible according to gender and dentition and also the significant correlation between the bone mineral density and the thickness of cortical bone.


Subject(s)
Female , Humans , Absorptiometry, Photon , Alveolar Process , Bicuspid , Bone Density , Dentition , Incisor , Mandible , Molar
13.
Korean Journal of Physical Anthropology ; : 199-206, 2010.
Article in Korean | WPRIM | ID: wpr-37919

ABSTRACT

Regarding to preserve the cadaver, formaldehyde has been used as a major preservative. However, the usage of formaldehyde has been considered by its harmful effects such as the disturbing ordor, toxicities and limitations to use. Therefore we studied the effect of decomposition-inhibition which is a natural product, grapefruit seed extract (GSE). Concerning the preservative activity, we sacrificed 8 week old male SD rat and collected liver. Using liver tissues, we treated GSE as a time dependant manner under 37degrees, 80+/-5% humidity conditions. To confirm GSE effects, we applied light and electron microscopic analysis. In results, we observed GSE attenuated the morphological changes and putrefaction of liver tissues more than 3 days. Herein, we introduced the potential substitute of formaldehyde to preserve the cadaver as well as animal tissues.


Subject(s)
Animals , Humans , Male , Rats , Cadaver , Citrus paradisi , Electrons , Formaldehyde , Humidity , Light , Liver , Seeds
14.
Korean Journal of Physical Anthropology ; : 141-153, 2010.
Article in Korean | WPRIM | ID: wpr-20905

ABSTRACT

As the interest in health is increasing and the population enjoyed the leisure sports is steadily increasing, the stress fracture, fracture or variant of metatarsal bone of foot has been shown frequently. The mistaken estimation about the length and rank of metatarsal bones during the osteotomy of the metatarsal bones of foot can be complicated. It is essential to have detailed knowledges about the anatomical structure of surgical region. This study aimed to investigate the metatarsal bones of foot and to develop a regression equation that can predict the length of metatarsal bones during the osteotomy. The subject of this study is fifty four feet (30M/224F). We measured the whole length and the article length of metatarsal bone. Also, we measured the whole width and the article width in the head, body, base of the metatarsal bone. The data was analyzed using SPSS win 13.0. The regression equation models of length of the metatarsal bones were developed by multiple regression analysis. The regression equation predicted first metatarsal length was second metatarsal articular length x0.770+7.780, second metatarsal length was third metatarsal length x0.976+6.050, third metatarsal length was fourth metatarsal length x1.000+0.922, fourth metatarsal length was third metatarsal length x0.917+4.167, fifth metatarsal length was fourth metatarsal length x0.901+7.972. The results of this study would be useful to clarify the characteristics of the metatarsal bone of the foot, to develop a regression equation for prediction of the length of the metatarsal bone.


Subject(s)
Foot , Fractures, Stress , Head , Leisure Activities , Metatarsal Bones , Osteotomy , Sports
15.
Korean Journal of Physical Anthropology ; : 155-167, 2010.
Article in Korean | WPRIM | ID: wpr-20904

ABSTRACT

Generally, osteoporosis is caused by the age-related physiologic bone loss. In cases of severe osteoporosis, the reduced bone mass and increaed skeletal fragility make the risk of fracture high. Recently, the relationship between the mandible and the condition of the skeletal bone, the molphometric data about the quality and quantity of the mandible are very important in the diagnosis and treatment of osteoporosis. This study was undertaken to clarify the correlations of the mineral density between mandible and other bones, to predict the bone mineral density (BMD) of the mandible. Cervical (2nd~5th) and lumbar (1st~4th) vertebrae, the bones of forearm and proximal femurs, mandibles from 42-embalmed cadavers (22/M, 20/F, mean age: 66.95 years) were examined. The BMD was measured by dual energy X-ray absorptiometry. The data were analysed with SPSS 12.0 program according to age, gender, to verify the statistical significance. 1. The BMD in the mandibular areas were variable. The BMD in the angle of the mandible (mean 0.40 g/cm2) was similar with that of ultra-distal part of the forearm (mean 0.34 g/cm2). 2. The BMD in the mandible was highly correlated with that of around the forearm. The correlation coefficient between density in the angle, the part between 1st and 2nd molar, the part between 1st molar and 2nd premolar and incisor portion of the mandible and density in the 1/3-distal part of the forearm was high (r=0.742, 0.697, 0.618, 0.652). The correlation coefficient between density in the part between 1st and 2nd premolar and density in the total femur was high (r=0.542). 3. The BMD in the mandible was correlated with that of around the femur neck (r=0.607). 4. The correlation coefficient between the BMD in the 1st and 2nd molar of the mandible and the BMD in the 5th cervical vertebra was high (r=0.543). And also, the correlation coefficient between the BMD in the incisor area of the mandible and the BMD in the 3rd lumbar vertebra was high (r=0.561). 5. Bony status of the mandible was classified into normal, osteopenia, osteoporosis by BMD of WHO criteria. The data represented that BMD of the mandible was decreased as BMD of the skeletal bones decreased. It is concluded that it could be possible to predict BMD of the mandible by the measurement of BMD in the forearm.


Subject(s)
Absorptiometry, Photon , Bicuspid , Bone Density , Bone Diseases, Metabolic , Cadaver , Femur , Femur Neck , Forearm , Incisor , Mandible , Molar , Osteoporosis , Spine
16.
Korean Journal of Ophthalmology ; : 183-187, 2009.
Article in English | WPRIM | ID: wpr-210147

ABSTRACT

PURPOSE: To identify the anatomy of the levator aponeurosis (LA) and Whitnall's ligament (WL) in Korean subjects using cadavers. METHODS: Orbital exenteration was performed in ten cadavers (20 eyeballs) that had no history of trauma near the eyeball. We observed characteristics of WL (tension, density, and shape) and the relationship between the superior rectus muscle (SR) and the levator palpebrae superioris. We measured the distance from both the eyelid margin and the upper border of the tarsal plate to the insertion of the LA medially, centrally, and laterally. RESULTS: The WLs we observed showed several shapes. In 12 eyes, we saw clear, white fibrotic bands, while in four others, we found thin, less taut bands. In four eyes, we were unable to identify the precise shape of the band. The insertions of the LA showed nasal dehiscence in 13 eyes and parallel attachment in seven eyes. The distances from the eyelid margin to the insertion of the LA medially, centrally, and laterally were 8.31 mm, 5.57 mm, and 5.15 mm, respectively. The distances from the upper border of the tarsal plate to the insertion of the LA medially, centrally, and laterally were 2.75 mm, 4.82 mm, and 4.29 mm, respectively. CONCLUSIONS: This study examined the anatomy of WL and the LA in Korean subjects and may be helpful as a reference in levator muscle surgery.


Subject(s)
Adult , Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Cadaver , Eyelids/anatomy & histology , Ligaments/anatomy & histology , Muscle, Skeletal/anatomy & histology , Oculomotor Muscles/anatomy & histology , Tendons/anatomy & histology
17.
Korean Journal of Physical Anthropology ; : 107-115, 2009.
Article in English | WPRIM | ID: wpr-103089

ABSTRACT

Identification of an individual is the mainstay of forensic investigation. In the present study, criteria for sex determination were established using 111 adult Korean sternums (63 males and 48 females). They were analyzed using identification points (IPs) and limit points (LPs), as reported previously. Only three of the ten parameters measured-sternal body length, sternal length (i.e., manubrium+body), and sternal area-were useful for sex determination, with sternal length being the most useful. The IP of sternal length was 147 and 130 mm for males and females, respectively. Using this parameter, 53.9% of males and 37.5% of females could be sexed with 100% accuracy. The LP was 141 mm for both males and females; sex could be discriminated accurately using this parameter in 85.7% of males and 85.4% of females


Subject(s)
Adult , Female , Humans , Male , Sternum
18.
Korean Journal of Physical Anthropology ; : 139-151, 2009.
Article in Korean | WPRIM | ID: wpr-103086

ABSTRACT

A balance between production and degradation of reactive oxygen species has an important role in the cardiovascular homeostasis, and is known to contribute to hypertension. Under oxidative stress, an upregulation of inducible NOS (iNOS) induces ischemic-reperfusion injury, and is involved in the pathophysiology of the hypertension. Ischemic-reperfusion injury of the skeletal muscle results from reactive oxygen species, and overexpression of iNOS in the skeletal muscle increases the ischemic injury. Superoxide dismutase (SOD), antioxidant, is a major enzyme for degradation of reactive oxygen species (ROS). The purpose of this study was to observe the effect ischemic preconditioning (IP) of the lower limb on the expression of iNOS, CuZnSOD and MnSOD in the white and red muscle of the spontaneously hypertensive rat (SHR). Nine weeks old male normotensive rat (Wistar-Kyoto rat, WKY) and SHR were divided into control and IP groups. The IP group was further divided into 3 (3IP) and 10 (10IP) times of IP. Left common iliac artery was occluded 3 and 10 times for 5 min of ischemia-5 min of reperfusion using rodent vascular clamp. The animals were sacrificed at 0, 0.5, 1, and 3 hours after reperfusion and the Tibialis anterior and Soleus were removed. The expressions of iNOS, CuZnSOD and MnSOD in the skeletal muscle were examined with immunohistochemical methods and Western blot analysis. iNOS was expressed in Tibialis anterior, but in Soleus after IP. The expression of iNOS was increased in both WKY and SHR, it was higher in SHR than WKY. CuZnSOD and MnSOD were expressed in Tibialis anterior and Soleus, higher in Soleus, after IP. The expression of CuZnSOD and MnSOD were increased in both WKY and SHR, higher in WKY than SHR. It is consequently suggested that hypertensive individual and white muscle are more sensitive to ischemic injury of the skeletal muscle as considering their high expression of iNOS and low expression of SODs.


Subject(s)
Animals , Humans , Male , Rats , Blotting, Western , White People , Homeostasis , Hypertension , Iliac Artery , Ischemic Preconditioning , Lower Extremity , Muscle, Skeletal , Muscles , Oxidative Stress , Rats, Inbred SHR , Reactive Oxygen Species , Reperfusion , Rodentia , Superoxide Dismutase , Superoxides , Up-Regulation
19.
Korean Journal of Physical Anthropology ; : 153-162, 2009.
Article in Korean | WPRIM | ID: wpr-103085

ABSTRACT

Akt, a key protein of cell survival, can promote cell growth and survival by activations of various cellular protective factors. Ischemic preconditioning (IP) has been known to reduce ischemic injury through upregulation of phosphorylation of Akt (p-Akt). CuZn-superoxide dismutase (SOD-1), an antioxidant enzyme, scavenges reactive oxygen species and protects cell from oxidative stress by increasing the activaiton of Akt. The present study was performed to examine the effects of IP on the expression of p-Akt and SOD-1 in the ischemicreperfused rat skeletal muscles. Thirty weeks old male SD rats were divided into 4 groups, such as controls, IP, 4 hour ischemia and 4 hour ischemia with IP. For IP, commom iliac artery was occluded three times for 5 min ischemia followed by 5 min reperfusion using rodent vascular clamps. Ischemia was induced by occlusion on the same artery for 4 hours. The Tibialis anterior and Soleus were removed at 0, 1, 3, and 24 hours of reperfusion. The expressions of p-Akt (Ser 473) and SOD-1 were examined with immunohistochemistry and Western blot analysis.In the IP group, the p-Akt and SOD-1 were increased, compared to the control group. In the ischemia group, the p- Akt and SOD-1 were decreased, compared to the control group, and were more abundant when reperfusion time were increased. IP increased the p-Akt and SOD-1 after 4 hour ischemia, and the p-Akt and SOD-1 were higher in Soleus compared to Tibialis anterior. These findings suggest that IP increases p-Akt and expression of SOD-1 in the ischemic-reperfused rat skeletal muscles, and that upregulations of p-Akt and SOD-1 induced by IP were higher in the red muscle fiber, Soleus, than the white muscle fiber, Tibialis anterior.


Subject(s)
Animals , Humans , Male , Rats , Arteries , Blotting, Western , Cell Survival , Iliac Artery , Immunohistochemistry , Ischemia , Ischemic Preconditioning , Muscle Fibers, Fast-Twitch , Muscle Fibers, Slow-Twitch , Muscle, Skeletal , Oxidative Stress , Phosphorylation , Reactive Oxygen Species , Reperfusion , Rodentia , Up-Regulation
20.
Korean Journal of Physical Anthropology ; : 1-11, 2008.
Article in Korean | WPRIM | ID: wpr-200842

ABSTRACT

The maxillary sinus is an anthropological landmark because of difference of volume of maxillary sinus among persons of different ethnic origin. This study was undertaken to provide morphological characteristics of Korean's maxillary sinus. The size and volume of maxillary sinus were measured in 44 Korean skulls 23 males and 21 females from cadaver. The shapes of the maxillary sinus were classified according to Anagnostopoulou et al. (1991) and the sizes of the medial antral wall area of the maxillary sinus were classified according to Fernandes (2004b). The mean volume of maxillary sinus was 12.83+/-5.41 cm3, which was significantly different between males and females. Bilateral symmetry of the volume of the maxillary sinus was observed in the 70% of the specimens. The width of maxillary sinus was 26.59+/-4.96 mm. The height of maxillary sinus was 26.65+/-6.16 mm. The anteroposterior length was 32.84+/-4.96 mm. The anteroposterior length measured on C-Arm radiography was 36.99+/-4.72 mm. The shape of maxillary sinus was semi-ellipsoid and the majority (50%) of the shape of medial antral wall area of maxillary sinus was the type of small. The results of this study could be useful to clarify the anthropological characteristics of the maxillary sinus in Korean, and to provide an index of ethnic classification for the medial antral wall area.


Subject(s)
Female , Humans , Male , Cadaver , Carbamates , Maxillary Sinus , Organometallic Compounds , Skull
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