Assessment of multiple drug resistance and sensitivity to helicobacter pylori in patients with peptic ulcer disease

Helicobacter pylori [H.pylori] is implicated in acute superficial gastritis, chronic atrophic gastritis, gastric carcinoma and MALT [Mucosa Associated Lymphoid Tissue] associated lymphoma. Prevalence of H. pylori antibiotic resistance is increasing and affect the efficacy of treatment. Our aim was to improve infected patients with new generations of antibiotics. 88 peptic ulcer patients recruited from gastroenerology and endoscopy unit at Suez Canal University, 5 gastric biopsies were taken from each and were examined for H. pylori by histopathology, rapid urease test and culture. Antibiotic susceptibility test was performed to: metronidazole, clarithromycin, amoxicillin, amoxicillin calvulanic acid, levofloxacin, tetracycline, ciprofloxacin, ofloxacin, ceftriaxone, cefoperazone and ceftazidime. Night-two% were positive for H. pylori, while 20% of the studied population were positively cultured .The results of antibiotic sensitivity were:100% of patients were sensitive to clindamycin, 87.5% sensitive to erythromycin, 68.8% sensitive to amoxicillin and levofloxacin, 50% sensitive to azithromycin and tetracycline, 25% sensitive to clarithromycin and 18.8% sensitive to amoxicillin and cavulanic acid. H. pylori resistance towards different antibiotics has increased. new promising results regarding clindamycin and erythromycin

Impact of Interleukin-28B gene polymorphism [rs12979860] on Egyptian patients infected with hepatitis C virus genotype-4

Single nucleotide polymorphisms [SNPs] in the Interleukin [IL]-28B gene, namely rs12979860, could predict response to pegylated interferon-alpha-ribavirin [PR] therapy in hepatitis C virus genotype 1 [HCV-1]-infected patients. A similar role was investigated in a case-control study conducted on 93 Egyptian patients chronically infected with HCV-4 in comparison to 22 individuals with spontaneous HCV clearance and 70 healthy volunteers. The homozygous C allele genotype [CC] was associated with sustained viral response [SVR] to therapy compared with the homozygous T allele genotype [TT] and the heterozygous genotype [CT]. In the SVR group, the response rate was statistically significantly higher in CC genotypes [58.6%] compared with CT/TT [20.3%]. There was no correlation between SVR patients' genotypes and early response to therapy or HCV baseline viral load. Our findings describe how IL-28B SNP genotyping may guide appropriate selection of HCV-4-infected patients for PR therapy. We underscore IL28B genotyping as a tool that might increase PR cost-benefit in Egypt

Serum level of beta- endorphin and some lipid parameters in patients with peptic ulcer

Endogenous opioid system like beta-endorphin have a functional role in modulating pain perception. This work was conducted to study the serum level of beta-endorphin and some lipid parameters in patients with peptic ulcer in relation to the site of ulcer, severity and duration of symptoms. The study included 48 patients with peptic ulcer and 15 healthy control. The serum level of beta-endorphin were significantly higher in patients with peptic ulcer than the control [0.475 +/- 0.058 Vs 6.18+1.004 ng/ml] [P<0.001] and the level were elevated in asymptomatic more than symptomatic patients[12.014+1.517 Vs 1.652 +/- 0.23 ng/ml] [P<0.001], acute more than chronic [7.459+1.4 Vs 3.621+0.81] with no difference between gastric and duodenal ulcer [5.284 +/- 1.11 Vs 7.076 +/- 1.67]. The lowest elevation were present in chronic symptomatic patients. Serum total, LDL cholesterol and triglyceride levels were elevated in patients with chronic peptic ulcer more than acute [P<0.001] with no differences between gastric and duodenal ulcer or between symptomatic and asymptomatic patients. Serum HDL-cholesterol levels were elevated in peptic ulcer patients who are acute more than chronic with no differences between gastric and duodenal ulcer or between symptomatic and asymptomatic patients

Metabolic disturbances accompanying hypervitaminosis D

75 Adult male albino rats were divided into 5 equal groups [15 each] and administered vitamin D[3] in different dose levels [500;1000; 20,000; 50,000 and 100,000 IU/kg b.w. daily] for four weeks. Fasting blood samples were collected before vitamin administration and 1, 2, 3 and 4 weeks post-treatment. Total lipids, cholesterol, triglycerides, phospholipids, total protein, albumin and alkaline phosphatase were estimated. The results revealed that vitamin D[3]administration caused a characterestic elevation in lipid parameters and hypoalbuminemia which were more apparent when high doses of the vitamin were given. Hypervitaminosis D[3]also caused a significant increase in serum alkaline phosphatase activity