ABSTRACT
Objective To observe the influence of sowing time and planting density on seed germination percentage and growth of Desmodium styracifolium(Osb.) Merr..Methods After quality inspection of the seed,the seeds planting test for Desmodium styracifolium(Osb.) Merr.was carried out from March to October,and the influences of sowing time and planting density on the seed germination percentage and growth were observed.Results On the planting base of Pingyuan county in Guangdong province,the seeds of Desmodium styracifolium(Osb.) Merr.planted from March to October had a high germination percentage and the seedling grew well.With the increase of planting density,the seed germination percentage decreased,but was up to 80% when the planting density was 0.5～1.0 g /m2.Conclusion The optimum planting time for Desmodium styracifolium(Osb.) Merr.is in autumn and the optimum planting density is 0.5～1.0 g /m2.
ABSTRACT
[Objective] To analyze the volatile constituents of Desmodium styracifolium (Osbeck) Merr. . [Methods] Volatile constituents of Desmodium styracifolium (Osbeck) Merr. were extracted by steam distillation and identified by gas chromatography-mass spectrometry (GC-MS) . [ Results ] Twenty-one kinds of components were separated and identified. [Conclusion] The method is reliable, stable and repeatable and can be used for the analysis of volatile constituents of Desmodium styracifolium (Osbeck) Merr. .
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Objective To establish the HPLC determination method of chlorogenic acid for developing the quality standard of Yinqiao Jiedu Oral Liquid.Methods HPLC method was performed on an E.Merck LiChrospher 100RP-18 column(250 mm? 4 mm,5 ? m).The mobile phase consisted of acetonitrle and o.4 % phosphoric acid(10:100).The flow rate was 1.0 mL/min,column temperature was 30 ℃,and the detection wavelength was 327 nm.Results Chlorogenic acid was linear at the range of 0.12～ 0.63 ? g(r=0.999 6),the average recovery was 96.68 %,and RSD was 1.56 % .Conclusion This method is accurate,reliable,reproducible,and can provide evidence for the quality control of Yinqiao Jiedu Oral Liquid.
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Objective To study the different extraction condition on naringin content in Drynaria extract.Methods The Naringin content in Drynaria extract was determined by HPLC.The e ffect of extraction solutions,drying temperature(60℃,100℃and 120℃)and drying time (0,0.5,1.0,1.5,2.0,5.0,10.0,25.0,50.0hours respectively)on naringin content in Drynaria extract was investigated.Results The optimal extraction solution for Drynaria extract was water,and the loss of naringin enhan ced with the increase of drying temperature and the prolongation of dryin g time.Con-clusion The result can provide references fo r the extraction technology,preparation selection and quality control of the compound including Rhizoma Drynariae.