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Objective To investigate the imaging performance and feasibility of 99Tcm labeled scFv against VCAM-1(99Tcm-scFv-VCAM-1) on atherosclerosis model rabbits.Methods HYNIC was used as a chelator for 99Tcm labeling.The labeling efficiency and radiochemical purity of 99Tcm-scFv-VCAM-1 were measured by instant thin layer chromatography after PD-10 purification.New Zealand white rabbits were employed for establishing atherosclerotic animal models by endothelia immunity injury and high fat diet, and plaques at aorta lesions were examined by HE staining.Model rabbits were sacrificed after administration of 99Tcm-scFv-VCAM-1 at 1 or 2 h respectively, and tissue samples were measured with gamma counter and weighted to obtain in vivo biodistribution data.Planar imaging was performed 1 and 2 h after the injection of 99Tcm-scFv-VCAM-1 to investigate radioactivity of abdominal aorta.After imaging study, atherosclerosis plaque and VCAM-1 expression at aortas were confirmed by the immunohistochemistry (IHC) study.Two-sample t test was used to analyze data.Results 99Tcm-scFv-VCAM-1 was successfully synthesized.Its labeling efficiency was 75%-83%, radiochemistry purity was (98.54±1.03)% and specific activity was 216 MBq/nmol.Atherosclerosis plaque was confirmed at the aortas of experimental rabbits by HE staining, while no plaque was observed in controls.Biodistribution data indicated that the tracer was cleared mainly through the kidneys.Planar imaging showed that the tracer uptake in abdominal aorta of model rabbits was higher than that of control rabbits, the T/B ratios at 2 h of the model group and control group were statistically different (3.68±0.73 vs 2.42±0.39;t=2.950, P<0.05;n=5).Atherosclerosis plaque and high level of VCAM-1 expression were observed at aortas of model rabbits by IHC study.Conclusions It is feasible and effective to detect vulnerable plaques using 99Tcm-scFv-VCAM-1.It may provide a promising way for early diagnosis and accurate evaluation of atherosclerosis.
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Objective@#To evaluate the daily airborne pollen concentrations and visiting rate of patients with allergic rhinitis (AR) and their correlation during 2012-2014 in Beijing.@*Methods@#Daily airborne pollen concentrations (55 998 numbers in total and 549 numbers in average) and its constitution from April to September each year (2012 to 2014) were compared. The number of patients with AR (44 203 in total) who visited the outpatient department of Otorhinolaryngology Head and Neck Surgery, Peking University Third Hospital between January 2012 and December 2014 was analyzed by month. Using SPSS 22.0 software, Kruskal-Wallis test was done for the comparison of visiting rate of patients with AR and airborne pollen concentrations. Correlation analysis between them was made as well.@*Results@#χ2 value of airborne pollen concentrations between different months in 2012 to 2014 was 110.7, 108.4 and 121.4, respectively; all P<0.01. The airborne pollen concentrations had two peaks per year, respectively: April to May, August to September. χ2 value of visiting rate of patients with AR between different months in 2012 to 2014 was 175.0, 185.1 and 134.5, respectively; all P<0.01. Visiting rate of patients with AR showed two scattering peaks each year, respectively: April to May, August to September. The highest pollen concentration of spring (April to May) was in early and middle April. Tree pollen was the major portion in spring, which were poplar pollen, pine tree pollen, ash tree pollen, cypress tree pollen and birch trees pollen. The highest pollen concentration of autumn (August to September) was in late August and early September. Weed pollen was the major portion in summer and autumn, which were artemisia pollen, chenopodiaceae pollen and humulus japonicas pollen. The visiting rate of patients with AR showed significant correlation with airborne pollen concentrations (r value was 0.537, 0.484 and 0.566, respectively; all P<0.01).@*Conclusion@#The visiting rate of patients with AR showed positive correlation with airborne pollen concentrations in recent three years.
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Objective To study whether the usage of antibiotics and white -blood -cell counts affect the effects of 2~12-year-old children with acute otitis media (AOM ) .Methods A total of 126 children (2~12 years old)with AOM ,from Jan 2013 to Dec 2013 ,were retrospectively analyzed .Usage of antibiotics and white -blood-cell count were recorded at first ,and the relationships between two factors and outcomes of children with AOM were studyed in one week ,one month and three months after treatment ,respectively .Results White blood cell counts were higher than normal in 59 .52% (75/126) 2~12 -year -old children with AOM ,and antibiotics were used in 73 .81% (93 /126) .Ninty three cases were followed -up until 3 month or more .Antibiotics were used in 74 cases ,and watchful waiting were used in the other 19 patients .The effective rates of two groups were 59 .45% and 52 .63% ,75 .68% and 84 .21% ,85 .14% and 78 .95% in one week ,one month and 3 months after treatment ,re‐spectively .There were no statistically significant differences(χ2 values were 0 .295 ,0 .903 ,0 .799 ,P>0 .05) .But the effective rates were signifantly higher in 1 and 3 months than that of in one week (P0 .05) between one and three months .Whether white-blood-cell count higher than normal or not ,the difference in efficacy was not statistically significant in 1 week ,1 month and 3 months after treatment (P >0 .05) .Conclusion In 2~12-year-old children with acute otitis media (AOM ) ,the final outcomes were inde‐pendent of the usage of antibiotics and white -blood-cell count .
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Infantile hemangiomas are the most common benign vascular tumors,accounting for 10％ of the incidence of benign tumors.Most hemangiomas may involute spontaneously,without any medical intervention.But partly because of the special parts of its growth,such as pediatric airway (subglottic),potentially it has fatality.So it is essential to give prompt diagnosis and appropriate treatment.Although there are multiple literatures have reported about the management of the subglottic hemangioma,there is no unified conclusion.This article summarizes a variety of methods on the diagnosis and treatment of infantile subglottic hemangioma.And the advantages and disadvantages of various treatments with the cases and the literature were assessed,so as to provide individualized treatment options for infantile.
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Objective To explore the effect of therapeutic touch(massage)on the neuTobehavior of preterm infants.Methods Sixty preterm infants( gestafional age in 31-36 weeks),through natural delivery or caesarean section were randomly assigned to therapeutic touch group(n=30)and control group(n=30).Both groups re-ceived routine pharmacothempy and nursing care and followed up for half a year from discharge.Touch group re-ceived additional whole-body massage.All subjects were evaluated with neonatal behavioral neurological assessment (NBNA)in one month after birth-and mental development index(MDI), psyehomotor development index(PDI)in 3 months and 6 months after birth.Results The NBNA scores(P<0.01),as well as MDI and PDI(P<0.05)were significantly higher in therapeutic touch group than the control.Conclusion Therapeutic touch intervention im-proves nervous system of preterm infants.
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BACKGROUND:There are neuronal precursors in subventricular zone (SVZ) of adult rat. The precursors migrate to olfactory bulb along the rostral migratory stream (RMS) and differentiate into local interneurons of olfactory bulb. But whether cutting the RMS would affect the migration of subventricular precursors is unknown.OBJECTIVE: To investigate the effects of cutting the olfactory peduncle on migration of subventricular zone cells in adult rat.DESIGN: Randomized controlled experiment.SETTING: College of Life Sciences, Univesity of Science and Technology of China; Staff Room of Anatomy, Xuzhou Medical College MATERIALS: The experiment was conducted in the Animal Experimental Center , Xuzhou Medical College from September 1997 to January 1999.Totally 25 male adult SD rats were recruited and randomly divided into olfactory peduncle-cutting 30 days group (n=10) and olfactory peduncle-cutting 60 days group(n=15, in which 5 rats were used for immonohisto chemistry).METHODS: 30 days and 60 days after being cutting olfactory peduncle,the rats were put to death by perfusion for fixation. Consecutive paraffin sections of brain were made. Cellular density and cross section area of RMS were measured. Quantitative analysis of cells in the RMS was performed with immunohistochemical method.MAIN OUTCOME MEASURES: The cellular density and cross section area of RMS, the expression of polysialylated neuronal cell adhesion molecule (PSA-NCAM)RESULTS: Totally 25 SD rats were recruited. Died for operation and been failure for cutting the olfactory peduncle, so altogether 13 rats used for analysis, 5 rats in 30days group and 8 rats in 60 days group (among that, 3 used for immunohistochemistry). ① Morphologyand quantitative change of RMS after cutting olfactory peduncle: The Nissl stained RMS cells were distinguished from adjacent tissue by the characterization of darken stained and dense distribution. There is little condensed nuclear or fragment nuclear. Cellular density at the operation side in the olfactory peduncle -cutting 30 days group and olfactory peduncle -cutting 60 days group was higher than that in the control side (P ＜ 0.01). Relative cellular density of RMS at surgical plane of operation side in the olfactory peduncle -cutting 60 days group was higher than that in the olfactory peduncle -cutting 30 days group(P ＜ 0.01 ). The cross section area of operation side in the two groups was both larger than that of the control side (P ＜ 0.01). There was no difference of cross section area between olfactory peduncle -cutting 60 days group and olfactory peduncle -cutting 30days group (P ＞ 0.05). ②Immunohistochemical reaction of RMS after cutting alfactory peduncle operation: 60 days after cutting olfactory peduncle, the accumulated cells in RMS were positive to polysialylated neuronal cell adhesion molecule.CONCLUSION: Cutting the olfactory peduncle results in the accumulation of neuronal precursors of RMS and increases the differentiation of migrating neuronal precursors.
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BACKGROUND: It is indicated in research that mildly modified low density lipoprotein (mm-LDL) is related to atherogenesis and it not only stores LDL and provides very strong biological activity, but also expresses many kinds of bioactive substances, like macrophage colony stimulating factor (MCSF) induced in cell culture and in animal body.OBJECTIVE: Differential display-PCR (DD-PCR) technique is used to study the genetic expressed difference in mm-LDL inducing vascular endothelial cells so as to lay the foundation for further explanation of the relationship between mm-LDL and arteriosclerosis.DESIGN: Repeated measurement was designed.SETTING: Taishan Medical College.MATERIALS: The experiment was performed in Basic Institute of Taishan Medical College from July 2003 to July 2004. Medium of human umbilical vein endothelial cells (HUVECs) was M199. Culture was done at 37 ℃, in 50 mL/L CO2. When cells grew to the fusion state, mm-LDL was added in the medium to the terminal concentration of 400 mg/L and then,induction was followed in 30 hours.METHODS: DD- reverse transcription (RT)-PCR technique was used to analyze genetic expression difference of human vascular endothelial cells induced with mm-LDL and reverse Northern analysis was performed to testify DD genetic fragments.mRNA in liver of mice.RESULTS: Human vascular endothelial cells induced with inm-LDL displayed some up and down-regulated genetic fragments. Up-regulated genes included thymosin 34, FGFRI protooncogene-chaperone protein, FK506 binding protein, rTSβ protein and intercellular adhesion molecule-1 (Ⅰ-CAM-1). Down-regulated genes included Apo bec-1 binding protein-l, cytochromeB561 and ERP72.CONCLUSION: DDRT-PCR testifies that mm-LDL induces changes of some genetic expression of human umbilicus vein endothelial cells in vitro and pathological changes of mm-LDL vascular endothelial cells, terminally results in atherogenesis.
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Objective To observe the changes in protein expression of NMDA receptor subunit NR1 in rat hippocampal subfields following transient forebrain ischemia. Methods immunohistochemistry and computer-assisted image analysis techniques were used. Results Early stage after reperfusion,NR1 protein expression levels were lower than that of sham control in all hippocampal subfields(P
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Objective The cells of subventricular zone in lateral wall of lateral ventricle of adult rats were localized. Method Immunohistochemistry. Results Polysialylated neuronal cell adhesion molecule and phosphotyrosine immunoreactive cells were found in subventricular zone, and they have same distributions, however, no any parvalbulin immunoreactive cells were found in subventricular zone. Conclusion neuronal precursor cells in subventricular and expression of phosphotyrosine are associated with proliferation of neuronal precursor cells in subventricular zone.
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In order to investigate the chemical and morphornetrical properties of the neuronal precursor cells derived from thesubventricular zone(SVZ) of the postnatal rat forebrain in vitro. The cell-type specific antibodies were used for the immunocy-tochemical staining ,and the morphometric parameters which were the mean soma diameter and the ellipticity index (i. e. , thesmallest soma diameter divided by the largest soma diameter) of every SVZ-derived cell were measured for identifying the pheno-types of the SVZ cells in vitro. The experiment animals were SD rats (weights: 100～ 150 g), the SVZ cells derived from thepostnatal rats were cultured on poly-D-lysine-coated 24-well glass chamber slides in the Neurobasal Medium supplemented withB27 in 5% CO2 at 37 C. The following results were obtained.. At 1 day in vitro, almost all SVZ cells (〉90%) from the postna-tal rat forebrain expressed Tujl, an antibody that recognizes neuron-specific tubulin. Likewise, the preponderance of the SVZcells expressed the polysialylated neural cell adhesion molecule (PSA-N-CAM) ; The majority of the SVZ Tujl-positive cells cul-tured were the cells that had oval-shaped bodies with two short, unbranched processes protruded from every two poles, theirmean soma diameter were 8.42±1.03μm and their ellipticity index were 0.57±0.12. Meanwhile, there were approximately20% of the SVZ cells in culture that were sphere-shaped cells with mean soma diameter 7.20±l.04 μm , and it might be observed that these cells connected with one another. As the time in culture went on, these sphere-shaped SVZ-derived cells alsotransformed to oval-shaped ones as described above, but it could be observed that the cells were still connected in the processesof them. By 3 and 5 days in culture, the SVZ cells had larger cell somas (average diameter 9. 07±1.07 μm), and often consider-ably longer processes but still with few branches. Immunocytochemical staining revealed that the majority of the SVZ cells in cul-ture remained Tujl-positive, PSA-N-CAM-positive. By 7 days in culture, the Tujl-positive cells in culture showed remarkablemorphological changes, and possessed typical neuronal phenotypes, which had more larger cell somas (average diameter 12.8 ±1.13 μm), and had more longer, branched processes. Our results showed that the SVZ in the postnatal SD rats contained theneuronal precursor cells which were PSA-N-CAM-positive and could differentiate into new neurons in vitro.
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The projections of diencephalon onto the rostral part of the mesencephalic periaqueductal gray matter (PAG) were investigated by using the method of retrograde transport of HRP in the rat. The results have led to following conclusions:1. The diencephalic afferents to the rostral part of PAG originate mainly from medial preoptic area, anterior hypothalamic area, dorsomedial and ventromedial hypothalamic nuclei, dorsal premamillary nucleus, lateral hypothalamic region, Forel's field and zona incerta ipsilaterally, and a few of them originate contralaterally.2. The anterior hypothalamic area, ventromedial hypothalamic nucleus, dorsal premamillary nucleus and zona incerta project more to the ipsilateral nucleus: lateralis of PAG than to the nucleus medialis and contralateral nucleus lateralis of PAG.3. In the anterior hypothalamic area, a large number of neurons projecting to PAG was located in the lateral anterior nucleus.4. In the lateral hypothalamic region, the lateral preoptic area and anterior division project to both nuclei lateralis and medialis, while its tuberal and mamillary divisions probably project only to nucleus lateralis.