ABSTRACT
This is the first report of the ultrastructure of the stigma and style during and after anthesis in Helianthus annuus L. using light and transmission electron microscopy. The stigma is bifid with unicellular papillae. There is no secretion of lipids, carbohydrates or proteins at anthesis. The style is semisolid in the upper portion, closer to the stigma, and becomes solid below. Ultrastructural changes on cells of the stigma and the style are described. The transmitting tissue of the ovule is first evident 40 minutes after pollination and persists during the first stages of embryogenesis. Only one pollen tube per micropyle was observed growing through this tissue.
Subject(s)
Flowers/ultrastructure , Helianthus/cytology , Helianthus/embryology , Microscopy, Electron, Transmission , Seeds/cytologyABSTRACT
Eleven Oxalis L. species from the province of Buenos Aires (Argentina) were investigated with scanning and transmission electron microscopes. We identified four different types and two subtypes of orbicules. We conclude that the close morphological similarity between these species is also reflected in their orbicules, and we suggest that the orbicules morphology may be a useful character in systematic studies.
Subject(s)
Magnoliopsida/anatomy & histology , Magnoliopsida/classification , Magnoliopsida/ultrastructure , Microscopy, Electron, Scanning , Microscopy, Electron, Transmission , Argentina , Species SpecificityABSTRACT
Pollen morphology and the degree of pollen variability within nine species and two varieties of Oxalis species from Buenos Aires Province (Argentina) were studied using light microscopy (LM), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Oxalis pollen grain is generally 3-colpate and the shape is prolate spheroidal, oblate spheroidal or spheroidal. The exine is microreticulate. The brochi are circular to polygonal; brochi sizes decrease near the colpi. The colpus membrane observed in unacetolysed material, is crustate or granular. The colpus length varies within a species; the largest grains are produced in stamens with long filaments and the smallest ones in stamens with short filaments. Four pollen types are distinguished by means of LM, SEM and TEM. They are recognized mostly on the basis of their colpus membrane structure. In conclusion, the morphological similarity among Oxalis species is also reflected in their pollen morphology.