ABSTRACT
@#Abstract：Objective To predict the structure and function of sterol O⁃acyltransferase 1（SOAT1）related to hepatocellular carcinoma（HCC）by using bioinformatics tools，in order to understand its mechanism as the marker and therapeutic target of S⁃Ⅲ subtype. Methods The structure，function and protein interaction of SOAT1 were predicted and analyzed by using databases or softwares such as NCBI，STRING，Protscale，SignalP，TMHMM，PSORT，SOPMA，SWISS ⁃ MODEL， NetNGlyc，NetOGlyc，Netphos and ProtParam. Results The protein encoded by SOAT1 was a hydrophobic protein with good stability，which was a nonclassical pathway protein with 8 transmembrane regions，mainly distributed among the cell membrane. SOAT1 was expressed in many tissues，while most of them in the adrenal gland，which showed multiple phosphorylation sites and was mainly involved in the synthesis and catabolism of cholesterol. Conclusion Bioinformatics analysis of structure and function of SOAT1 showed that SOAT1 lipid synthesis and catabolism pathways played an important role，and lipid expression was closely related to the development of cancer，indicating that the treatment of HCC may be achieved by regulating the expression of SOAT1 gene.
ABSTRACT
A rapid, sensitive and specific reversed-phase high-performance liquid chromatographic method was developed for the determination of 3-n-butylphthalide, a drug currently being developed for treatment of stroke, in mice tissue. Ultraviolet detection were monitored at 228 nm for quantification of 3-nbutylphthalide. Ibuprofen was used as internal standard. The peak area ratio vs concentration in tissue was linear over the range of 25.625–1025.000 ng/mL and the limit of quantification was 25.625 ng/mL. The method was successfully applied to pharmacokinetic investigation in mice. The mean distribution half-life was 5.471 ± 4.736 min and elimination half-life was 58.459 ± 34.370 min. Finally, a preclinical biodistribution research of 3-n-butylphthalide in mice following intravenous administration had been studied. Brain targeting of 3-n-butylphthalide was strong and metabolism in the liver and blood was rapid.
ABSTRACT
T cell can only recognize specific antigenic peptide-MHC complex on antigen-presenting cell. This is MHC restriction in antigen recognition of T cell. This phenomenon was discovered by an Austrian scientist Peter. C. Doherty and a Swedish scientist Rolf. M. Zinkernagel by chance. Then, in order to explain the phenomenon, they proposed two hypotheses: dual receptor and modified self. In the during following 20 years numbers of scientists spent great amount of time in the study of the phenomenon. The process of cell-mediated immune response becomes clear, which greatly promotes the advancing of immunology and many related disciplines.