ABSTRACT
Objective:To seed for stable time window of the integrated disease-syndrome animal model based on the counterevidence from Chinese medicinal prescriptions， and to verify syndrome stability and reliability. Method:A model of depression was established by exposing rats to chronic unpredictable mild stress （CUMS）， followed by body weight measurement， sugar water test， behavioral test， and brain 5-hydroxytryptamine（5-HT） detection. The identification of liver depression and spleen deficiency syndrome was conducted after the equivalent transformation of human clinical symptoms into macroscopic representations of rats. Based on the dynamically collected macroscopic representation scale， Xiaoyaosan was used to reversely verify the stability and reliability of the integrated disease-syndrome animal model of depression due to liver depression and spleen deficiency. Result:The sugar water consumption and the number of crossings and the total movement distance in the open field test of 16-week-old rats in the CUMS （eight weeks of CUMS） group were significantly lower than those in the normal group （<italic>P</italic><0.05）. According to the immunohistochemical results， the 5-HT content in hippocampal area CA2 of rats in the CUMS group was also significantly lowered as compared with that in the normal group（<italic>P</italic><0.05），which indicated that depression was successfully modeled. The liver depression and spleen deficiency syndrome was present in 14-week-old rats （six weeks after CUMS）of the CUMS group， and the number of rats experiencing the liver depression and spleen deficiency syndrome reached the peak in the 16th week （eight weeks after CUMS），accounting for 70% of the total number. Thereafter， the number decreased gradually. The syndrome scores of the 14-， 16-， 18-， 20-， and 22-week-old rats in the Xiaoyaosan group were reduced by 66.6%， 70.7%， 54.8%， 50.4%， and 44.8%， which were graded as effective， marked effective， effective， effective， and effective， respectively. Conclusion:The age of 14-16 weeks（six to eight weeks after CUMS） is considered the stable and reliable time window for depression due to liver depression and spleen deficiency.
ABSTRACT
Objective To explore the preliminary experience of a one-stage hybrid operating room (OR) in cerebrovascular surgery.Methods A total of 23 patients [9 male,mean age:(40.0±11.2) years] underwent one-stage hybrid approach in a hybrid OR from September 2012 to December 2013,were chosen in our study.Craniotomy and percutaneous intervention of these patients were performed in a single session.Their clinical data were retrospectively analyzed.Results Thirty-one times of digital subtraction angiography (DSA) was performed in all patients,and 15 patients were diagnosed as having intracranial aneurysms and 8 arteriovenous malformations (AVMs).In one patient,a reposition of the clip was needed due to neck remnant after clipping.Residual nidus resection was done in 2 patients with AVMs.Temporary balloon occlusion happened in 5 patients,parent artery occlusion in 3,and 8 accepted emergency surgery under DSA confirming cerebrovascular diseases and removing hematoma.No procedural complications was observed.Conclusion A combined endovascular and surgical approach conducted in a one-stage hybrid OR provides a new strategy for the treatment of complex and emergency cerebrovascular diseases.