Abietane diterpenoids and iridoids from Caryopteris mongolica / 中国天然药物

Six new abietane diterpenoids (1-6) and five undescribed iridoids (7-11) have been isolated from the aerial parts of Caryopteris mongolica. The intricate structural characterization of these compounds was meticulously undertaken using an array of advanced spectroscopic techniques. This process was further enhanced by the application of DP4+ probability analyses and electronic circular dichroism (ECD) calculations. Following isolation and structural elucidation, the cytotoxicity of these compounds was evaluated. Among them, compound 3 stood out, displaying significant cytotoxic activity against HeLa cells with an IC50 value of 7.83 ± 1.28 μmol·L-1. Additionally, compounds 1, 2, 4, 9, and 10 manifested moderate cytotoxic effects on specific cell lines, with IC50 values ranging from 11.7 to 20.9 μmol·L-1.

Chemical constituents from Imperata cylindrica / 中国中药杂志

Chemical investigation of Imperata cylindrica led to the isolation of thirteen compounds using various chromatographic techniques. The structure of these compounds were identified as: three phenylpropanoids, 1-(3,4,5-trimethoxyphenyl)-1,2,3-propanetriol ( 1 ), 1-O-p-coumaroylglycerol (2), 4-methoxy-5-methyl coumarin-7-O-beta-D-glucopyranoside (3); four organic acids, 4-hydroxybenzene carboxylic acid(4), 3,4-dihydroxybenzoic acid (5), vanillic acid (6), 3, 4-dihydroxybutyric acid (7); one phenolic compound, salicin (8); and five triterpenes, namely, arundoin (9), cylindrin (10), fernenol (11), simiarenol (12), glutinone (13) by their physicochemical properties and spectral data analysis. Among them, compounds 1-8 were isolated from the genus Imperata for the first time.

Chemical constituents of aerial parts of Gelsemium elegans / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents from the aerial parts of Gelsemium elegans.</p><p><b>METHOD</b>Compounds were isolated and purified by repeated column chromatography, as well as semiprep arative HPLC, and their structures were identified by physicochemical properties and spectroscopic methods, such as NMR and MS.</p><p><b>RESULT</b>Sixteen compounds were obtained and identified from G. elegans, including nine alkaloids: koumine (1), gelsenicine (2), 19-(Z)-akuammidine (3), gelsemoxonine(4), gelsemin (5), gelsevirine (6), humantenine (7), 11-methoxygelsemamide (8) and gelegamine D (9). Three megastigmane glycosides: (3R, 5S, 6S, 7E, 9R)-megastigman-7-ene-3, 5, 6, 9-tetrol-9-O-beta-D-glucopyranoside (10), (6R, 7E, 9R)-9-hydroxy-4, 7-megastigmadien-3-one-9-O-[alpha-L-arabinopyranosyl-(1 --> 6)-beta-D-glucopyranoside] (11) and (6S, 7E, 9R) -6, 9-dihydroxy-4, 7-megastigmadien-3-one-9-O-[alpha-L-arabinopyranosyl-(1 --> 6) -beta-D-glucopyranoside] (12). Two flavone C-glycosides: orientin (13) and isorientin (14); one iridoid glycoside, sweroside (15) and one fructoside, n-butyl-alpha-D-fructofuranoside (16).</p><p><b>CONCLUSION</b>Compounds 10-16 were isolated from the genus Gelsemium for the first time.</p>

Chemical constituents from Senecio nemorensis / 中国药科大学学报

To study the chemical constituents of Senecio nemorensis.,12 known compounds were isolated and identified,which were niacinamide(Ⅰ),vanillin(Ⅱ),syringic acid(Ⅲ),syringalddehyde(Ⅳ),3-acetyl-4-hydroxy-benzoic acid(Ⅴ),4,4-dimethyl-1,7-heptanedioic acid(Ⅵ),1H-indole-3-carboxaldehyde(Ⅶ),ethyl caffeate(Ⅷ),1-0-(E)-p-methoxycinnamoyl-β-D-glucopyranoside(Ⅸ),(6S,7E)-6-hydroxy-4,7-megastigmadien-3,9-dione(Ⅹ),Annuionone D(Ⅺ),and(1 'S,6'R)-abscisic acid(Ⅻ),respectively.

One new galloyl glycoside from fresh leaves of Psidium guajava L / 药学学报

To investigate the chemical constituents of Psidium Guajava L, the EtOH/H2O extract of the fresh leaves was subjected to various chromatography. Five constituents with galloyl moiety were isolated and elucidated as 1-O-(1, 2-propanediol)-6-O-galloyl-beta-D-glucopyranoside (1), gallic acid (2), ellagic acid (3), ellagic acid-4-O-beta-D-glucopyranoside (4) and quercetin-3-O-(6"-galloyl) beta-D-galactopyranoside (5) by spectroscopic methods, including 2D NMR and HR-ESI-MS spectrometry as well as by comparison with published data. Compounds 4 and 5 were obtained from P. guajava for the first time, and compound 1 is a new polyhydroxyl compound.

A new phenylpropanoid glycoside from Cirsium setosum / 药学学报

To study the chemical constituents of Cirsium setosum (Willd.) MB., 70% ethanol extract of the aerial parts was subjected to column chromatography. One new phenylpropanoid glycoside, sinapyl alcohol 9-O-(E)-p-coumaroyl-4-O-beta-D-glucopyanoside (1) was isolated, along with three known compounds: lycoperodine-1 (2), apigenin-7-O-(6"-(E)-p-coumaroyl)-beta-D-galactopyranoside (3) and quercetin (4). The structures were elucidated on the basis of spectral and chemical evidence. Compound 2 was obtained from Cirsium genus for the first time, compounds 3 and 4 were obtained from this plant for the first time.

Quality specification of Perillae Fructus / 中国中药杂志

To improve the quality control specification of Perillae Fructus, the identification methods and assay were developed. Rosmarinic acid, luteolin and apigenin in the sample were identified by TLC. The content of rosmarinic acid was determined by HPLC. The linear calibration curve of rosmarinic acid was obtained in the ranges of 19.4-194.2 g x L(-1) (R2 = 0.9999). The arerage coveriy (n=9) for the assay was 99.8% (RSD 3.6%). The established methods are accuracy, sensitivity and reproducible, and can be used for the quality control of Perillae Fructus.

Qualitative and quantitative methods for Alpiniae Katsumadai semen / 中国中药杂志

The qualitative and quantitative methods for the quality evaluation of Alpiniae Katsumadai Semen were established. Alpinetin, pinocembrin, cardamonin and alnustone in the sample were identified by TLC. The contents of them were determined by HPLC. The separation was performed on a Ultimate XB-C18 column (4.6 mm x 250 mm, 5 microm) at 30 degrees C using a gradient elution consisting of mobile phase A (water) and mobile phase B (MeOH). The detection wavelength was 300 nm. The TLC method was suitable for the identification of alpinetin, pinocembrin, cardamonin and alnustone. The linear ranges of the four reference compounds were 25.5-509 (r = 0.9999), 24.9-498 (r = 0.9999), 26.1-521 (r = 0.9999), 50.2-1003 ng (r = 0.9999), respectively. The average recoveries (n=9) of the four components were 97.95%, 97.36%, 97.50%, 98.02%, RSD < 1.9%. Nine samples were analyzed by the established methods. The results indicate that, the methods are simple, accurate, sensitive and reliable for quality evaluation of Alpiniae Katsumadai Semen.

Investigation on quality standard of Franchet groundcherry / 中国中药杂志

<p><b>OBJECTIVE</b>To develop identification and assay methods of Franchet groundcherry.</p><p><b>METHOD</b>TLC method was used to identify of physalin L in the sample using high performance silica gel G plate and a mixture of chloroform-acetone-methanol (25:1:1) as a developing solvent. In the chromatogram, physalin L showed a distinct fluorescence spot under UV 365 nm with good separation. In the HPLC method, luteoloside was separated on a Venusil XBP C18 (4.6 mm x 250 mm, 5 microm) column with acetonitrile-0. 2% phosphoric acid (20:80) as the mobile phase with flow rate of 1 mL x min(-1). The detection wavelength was set at 350 nm.</p><p><b>RESULT</b>For the HPLC quantitation method, the calibration curve of luteoloside displayed ideal linearity over the range of 0.50-249.40 mg x L(-1) with the regression equation of Y = 55,313X + 3.1641 (r = 1.000). The average recovery of luteoloside was 98.79% with a RSD of 1.1%. And the intra-day and inter-day precisions were less than 2%.</p><p><b>CONCLUSION</b>The TLC identification and HPLC determination were sensitive, reliable and repeatable and can be applied for the quality evaluation and assessment of Franchet Groundcherry Calyx.</p>

Alkaloids from Senecio scandens / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the alkaloids from Senecio scandens.</p><p><b>METHOD</b>Compounds were isolated with silica gel and Sephadex LH-20 column chromatography and their structures were determined by spectral analysis and chemical evidence. The hepatic cytotoxicity of isolated compounds was tested by MTT method in vitro.</p><p><b>RESULT</b>Six alkaloids were obtained and identified as adonifoline (1), 7-angeloylturneforcidine (2), hordenine (3), 1, 3, 6, 6-tetramethyl-5, 6, 7, 8-tetrahydro-isoquinolin-8-one (4), 4-(pyrrolidin-2-one) -phenyl acetic acid (5), (4-pyrrolidinophenyl) acetic acid (6).</p><p><b>CONCLUSION</b>Compound 6 is a new natural product, compounds 3, 4 were obtained from the genus Senecio for the first time, compounds 2, 5 were obtained from this plant for the first time. Compound 1 showed significant growth inhibitory effect against hepatocyte at 100 micromol x L(-1).</p>

Determination of kirenol in Herba Siegesbeckiae Preparata by high performance liquid chromatography / 中国中药杂志

<p><b>OBJECTIVE</b>To establish a high performance liquid chromatographic method for the determination of kirenol in Herba Siegesbeckiae Praeparata.</p><p><b>METHOD</b>The analysis was carried out on a Boston Crest ODS column (4.6 mm x 250 mm, 5 microm) with gradient elution using acetonitrile-water as mobile phases. The flow rate was 1.0 mL x min(-1) and the detection wavelength was at 215 nm.</p><p><b>RESULT</b>The calibration curve was linear over the range of 0.0202-20.02 microg for kirenol. The correlation coefficient of the calibration curve was 0.99975. The average recovery was 99.62% with relative standard derivation (RSD) of 2.0%.</p><p><b>CONCLUSION</b>The results showed that the method is simple, accurate and repeatable and it is suitable for the determination of kirenol in Herba Siegesbeckiae Praeparata.</p>

Triterpenoid constituents in fruits of Psidum guajava / 中国中药杂志

The chemical constituents from the fruit of Psidium guajava were investigated. Nine triterpenoids, ursolic acid (1), 1beta, 3beta-dihydroxyurs-12-en-28-oic acid (2), 2alpha,3beta-dihydroxyurs-12-en-28-oic acid (3), 3beta,19alpha-dihydroxyurs-12en-28-oic acid (4), 19a-hydroxylurs-12-en-28-oic acid-3-O-alpha-L-arabinopyranoside (5), 3beta, 23-dihydroxy urs-12-en-28-oic acid (6), 3beta, 19alpha, 23beta-tri-hydroxylurs-12-en-28-oic acid (7), 2alpha, 3beta,19alpha, 23beta-tetrahydroxyurs-12-en-28-oic acid (8), 3alpha,19alpha,23,24-tetrahydroxyurs -12-en-28-oic acid (9) were isolated by means of chromatography, and their structures were elucidated on the basis of MS, H NMR, 13C-NMR spectra. Compounds 2, 5-9 were isolated from this plant for the first time.

Non-alkaloid constituents of Gelsemium elegans / 中国中药杂志

<p><b>OBJECTIVE</b>To study the non-alkaloid chemical constituents of Gelsemium elegans.</p><p><b>METHOD</b>Compounds were isolated and purified by repeated column chromatography, and their structures were elucidated by spectroscopic methods.</p><p><b>RESULT</b>Ten compounds were isolated and their structures were identified as tamarixin (1), tamarixetin 3-O-beta-D-galactopyranoside (2), scopolin (3), scopoletin (4), uradine (5), caffeic acid (6), caffeic acid ethyl ester (7), ferulic acid ethyl ester (8), ethyl-alpha-D-fructofuranoside (9), and ethyl-beta-D-fructopyranoside (10).</p><p><b>CONCLUSION</b>Compounds 1-3,5-10 are firstly isolated from this plant and compounds 1, 2, and 5-10 are isolated from the genus Gelsemium for the first time.</p>

Determination of norisoboldine in Radix Lindera by RP-HPLC / 中国中药杂志

<p><b>OBJECTIVE</b>To develop a RP-HPLC method for quantitative determination of norisoboldine in Radix Linderae and to provide valuable data for quality control of Radix Linderae.</p><p><b>METHOD</b>The separation was performed on a Phenomenex Gemini C18 column (4.6 mm x 250 mm, 5 microm) at 25 degrees C using a gradient elution of mobile phase A (0.5% formic acid, adjusted pH 2.25 with triethylamine) and mobile phase B (acetonitrile). The detection wavelength was 280 nm.</p><p><b>RESULT</b>The calibration curve showed a good linearity (r = 0.999 9) within test ranges of 0.015-1.509 microg. The average recovery was 99.58% with RSD 1.4%.</p><p><b>CONCLUSION</b>The developed method is simple, accurate and reliable with good repeatability. It is suitable for quality evaluation of Radix Linderae.</p>

Fingerprinting Analysis of Radix Salviae Miltiorrhizae with Ultra-high Performance Liquid Chromatography / 广州中医药大学学报

Objective To establish an ultra-high performance liquid chromatographic fingerprinting analysis method for the hydrophilic component of salvianolic acids and the lipophilic component of tanshinones in Radix Salviae Miltiorrhizae(RSM),and to supply a rapid and scientific method for quality control of RSM.Methods The chromatographic conditions were as follows: Acquity C18 BEH(2.1mm?100 mm,1.7 ?m)chromatographic column at 30℃,gradient elution with acetonitrile(A) and 0.4% formic acid(B)(90%~50% A for 0~10 min,75% A for 15 min),the flow rate being 0.5 mL?min-1 and UV detection wavelength set at 280nm.Results The chromatograms of the hydrophilic component and the lipophilic component were obtained within 15min,peak volume being 85.There were 20 peaks separated completely and identified by using HPLC-diode array detection-electrospray ionization-mass spectrometry.Conclusion The developed method is proved practicable,rapid and reliable for quality control of RSM and its preparations.