ABSTRACT
Objective: Oral lichen planus [OLP] is a chronic inflammatory disease. Immunological factor may act as etiological factor. The cellular immune cells such as T cells are important in pathogenesis. Interferon gamma [IFN-Gamma] and interleukin 4 [IL-4] are secreted by T-helper 1 [Th1] and Th2, respectively. The aim of this study was to investigate the correlation between salivary levels of IFN-Gamma and IL-4 with OLP
Materials and Methods: This case control study included sixty three Iranian OLP patients who were selected from the Department of Oral Medicine of Ahvaz Jundishapur University of Medical Sciences from January to July 2013. An equal number of healthy volunteers were also selected as a control group. The OLP patients were then divided into two following sub-groups: reticular [n=30] and erythematous/ulcerative [n=33]. All patients had no systemic disease and received no medication. IFN-Gamma and IL-4 levels in whole unstimulated saliva [WUS] were measured using the enzyme-linked immunosorbent assay [ELISA] test. Data analysis was done using t test, ANOVA, least significant difference [LSD] test, and the Kruskal-Wallis test
Results: Reticular OLP patients showed higher salivary IFN-Gamma [7.74 +/- 0.09 pg/ml] and IL-4 [3.876 +/- 0.05 pg/ml] levels compared with the control group, indicating that difference was significant. Salivary IFN-Gamma/IL-4 ratio significantly increased compared with control group [P=0.042]. Salivary IFN-Gamma and IL-4 levels between sub-groups [reticular and erythematous/ulcerative] were not significantly different [2.6 +/- 0.06 and 2.3 +/- 0.05, respectively, P<0.05]
Conclusion: Salivary IFN-Gamma and IL-4 levels were increased in OLP patients. An increase of salivary IFN-Gamma/IL-4 ratio in OLP patients showed that Th1 might have a dominant role in the OLP pathogenesis
ABSTRACT
Objective: Chronic periodontitis is the most common form of periodontal disease. Changes in biomarkers seem to be associated with the disease progression. Procalcitonin [PCT] is one of these biomarkers that are altered during infection. This study was established to investigate the relationship between periodontitis as an infectious disease and salivary PCT
Materials and Methods: This case-control study was performed on 30 patients with generalized chronic periodontitis and 30 health individuals as control group who were referred to Dental School, Jundishapur University of Ahvaz, Ahvaz, Iran at Feb to Apr 2014. The saliva samples were collected and analyzed by the enzyme-linked immunosorbent assay [ELISA] method. Data analysis was performed using t test with the SPSS [SPSS Inc., Chicago, IL, USA] version 13
Results: In both groups, age and sex distribution values were not significantly different. The concentrations of salivary PCT in controls and patients ranged from 0.081 pg/ mL to 0.109pg/mL and from 0.078pg/mL to 0.114pg/mL, respectively. The statistically significant differences between the two groups were not observed [P=0.17]
Conclusion: It seems that salivary PCT concentration is not affected by disease progression. Therefore, PCT is not a valuable marker for the existence of periodontal disease
ABSTRACT
Periodontal disease is a chronic bacterial infection that affects the tissues supporting the teeth. Our effort in improving periodontal disease is to reduce destructive mediators. One of the Biomarkers is osteoprotegrin [OPG]. OPG is a protein which prevents osteoclast activity. The aim of this study was evaluationof osteoprotegrin [OPG] concentration in saliva of patients with generalized moderate chronic periodontitis and comparing it with that of healthy individuals. This study was carried out on 30 patients [15 patients with chronic periodontitis and 15 patients as the control group] referred to Ahvaz Jundishapour dental school. Saliva samples were collected and examined by ELISA. The data were analyzed by t-test. Mean OPG concentration in patients and control group were 117.63 +/- 84.13 mg/dl and 210.81 +/- 170.69 mg/dl respectively. The difference was not significant. It appears that OPG concentration in saliva cannot be used as a marker for the assessment of periodontal disease
Subject(s)
Humans , Chronic Periodontitis , Saliva , Enzyme-Linked Immunosorbent AssayABSTRACT
PURPOSE: The effects of magnetostrictive and piezoelectric devices on tooth surfaces seem to differ with regard to the root surface roughness they produce. This study aimed to compare the results of scaling using magnetostrictive and piezoelectric devices on extracted teeth. METHODS: Forty-four human extracted teeth were assigned to four study groups (n=11). In two groups (C100 and C200), the teeth were scaled using a magnetostrictive device and two different lateral forces: 100 g and 200 g, respectively. In the other two groups (P100 and P200), the teeth were scaled with a piezoelectric device with 100 g and 200 g of lateral force, respectively. he teeth were scaled and the data on the duration of scaling and the amount of surface were collected and analyzed using the t-test. RESULTS: The mean time needed for instrumentation for the piezoelectric and magnetostrictive devices was 50:54 and 41:10, respectively, but their difference was not statistically significant (P=0.171). For root surface roughness, we only found a statistically significantly poorer result for the C200 group in comparison to the P200 group (P=0.033). CONCLUSIONS: This study revealed that applying a piezoelectric scaler with 200 g of lateral force leaves smoother surfaces than a magnetostrictive device with the same lateral force.