ABSTRACT
<p><b>OBJECTIVE</b>To investigate the anti-tumor effect of monocytes/macrophages against Tca8113 cells and the secretion of vascular endothelial growth factor (VEGF) in acid microenvironment in vitro.</p><p><b>METHODS</b>Peripheral blood mononuclear cells were extracted from healthy person's blood and cultivated to transform into monocytes/macrophages. The monocytes/macrophages were cultured with Tca8113 in acid microenvironment (pH6.6 and pH6.8) and in normal microenvironment (pH7.2). The anti-tumor effect of monocytes/macrophages against Tca8113 cells were examined by MTT assay. The expression of VEGF was detected by enzyme-link immunoassay (ELISA).</p><p><b>RESULTS</b>The anti-tumor effect of monocytes/macrophages against Tca8113 cells in acid environment was lower than in normal environment (P < 0.05). VEGF excreted by mononuclear/macrophage was significantly higher in acid microenvironment than in normal microenvironment.</p><p><b>CONCLUSION</b>Due to acid microenvironment inside tumor, the anti-tumor effect of monocytes/macrophages against tumor cell was decreased, but the secretion of VEGF was gradually increased. However, the function of monocytes/macrophages on anti-tumor need more research.</p>
Subject(s)
Humans , Cell Line, Tumor , Coculture Techniques , Culture Media , Chemistry , Hydrogen-Ion Concentration , Macrophages , Metabolism , Monocytes , Metabolism , Vascular Endothelial Growth Factor A , MetabolismABSTRACT
A 10-month-old boy suffering from a rare multiple-deformities with congenital cleft lip, ala nasi cleft, face horizontal cleft, alveolar cleft, accessory ear and inguinal hernia. All of the above-mentioned multiple-deformities have been cured by surgical operations, during which the bleeding was prevented strictly. According to the principle of asepsis, the hernial sac was ligatured firstly, the accessory ears were cut off, the ala nasi cleft, cleft lip and facial transversal cleft were repaired in turn. After the operation, the wound were completely healed up in time and the deformities were corrected.