ABSTRACT
ObjectiveTo investigate the effect of Guiqi Baizhu prescription （GQBZ） combined with oxaliplatin on the expression of epidermal growth factor receptor （EGFR） and vascular endothelial growth factor receptor-2 （VEGFR2） and angiogenesis in gastric cancer-bearing mice. MethodThe tumor-bearing model of gastric cancer was induced in Kunming mice. The mice were randomly divided into blank group， model group， oxaliplatin group （10 mg·kg-1）， and high- （17.68 g·kg-1）， medium- （8.84 g·kg-1）， and low-dose （4.42 g·kg-1） combination groups （GQBZ combined with oxaliplatin）. After the last administration， the transplanted tumor was collected and the tumor inhibition rate was calculated. Hematoxylin-eosin （HE） staining was used to observe the morphological changes of tumor tissues. Enzyme-linked immunosorbent assay （ELISA） was used to detect the serum content of epidermal growth factor （EGF）， interleukin-8 （IL-8）， and vascular endothelial growth factor （VEGF）. Western blot and immunohistochemistry （IHC） were used to detect the expression of EGFR， phosphorylated EGFR （p-EGFR）， VEGFR2， phosphorylated VEGFR2 （p-VEGFR2）， and platelet-endothelial cell adhesion molecule （CD31）. Real-time fluorescence-based quantitative polymerase chain reaction （Real-time PCR） was used to detect the mRNA expression of EGFR and VEGFR2. ResultThe tumor weight in the drug intervention groups was significantly lower than that in the model group （P<0.01）. Compared with the oxaliplatin group， the high- and medium-dose combination groups showed reduced tumor weight （P<0.05， P<0.01）. The tumor cells in the model groups were high in cell density and regular in shape， and no clear tissue necrosis was seen. The tumor cell density in the drug intervention groups was reduced， and clear tissue necrosis and large-scale inflammatory cells were visible. Compared with the blank group， the model group and the drug intervention groups showed increased serum levels of EGF， VEGF， and IL-8 （P<0.05， P<0.01）. Compared with the model group， the drug intervention groups showed decreased serum levels of EGF， VEGF， and IL-8 （P<0.01）， reduced protein expression of EGFR， p-EGFR， VEGFR2， p-VEGFR2， and CD31， and declining mRNA expression of EGFR and VEGFR （P<0.01）. Compared with the oxaliplatin group， the high- and medium-dose combination groups showed decreased serum levels of EGF， VEGF， and IL-8 （P<0.05， P<0.01）， reduced protein expression of EGFR， p-EGFR， VEGFR2， p-VEGFR2， and CD31， and dwindled mRNA expression of EGFR and VEGFR2 （P<0.05， P<0.01）. The low-dose combination group showed decreased serum levels of EGF， VEGF， and IL-8， reduced protein expression of EGFR， p-EGFR， VEGFR2， p-VEGFR2， and CD31， and dwindled mRNA expression of EGFR and VEGFR2， but the difference was not statistically significant. ConclusionGQBZ combined with oxaliplatin can inhibit the growth and angiogenesis of tumor tissues in gastric cancer-bearing mice by affecting the expression of EGFR and VEGFR2.
ABSTRACT
Kaposi's sarcoma, non-hodgkin's lymphoma and invasive cervical was named AIDS-related cancer. This article introduces the appeal tumor research progress of modern medicine, and raises that antivirus, immune reconstitution and antitumor are the key factors in the treatment of AIDS-related cancer. Traditional Chinese medicine may play a role in the following aspect: relieve antiviral drug adverse reaction, have some antiviral effect,adjust the immune function,enhance efficacy and decrease toxicity of radiotherapy. According to the patients' immune function and the treatment phase, etc. It will contribute to improve the patients'lives and prolong the survival period to establish reasonable and comprehensive treatment strategies and to combine holistic therapy with individual therapy.
Subject(s)
Humans , Acquired Immunodeficiency Syndrome , Antineoplastic Agents , Medicine, Chinese Traditional , Methods , Neoplasms , Drug Therapy , Allergy and Immunology , TherapeuticsABSTRACT
<p><b>OBJECTIVE</b>To evaluate the application of multi- slice spiral computed tomography (MSCT) in the preoperative staging of gastric carcinoma.</p><p><b>METHODS</b>Eighty- nine cases of gastric carcinoma confirmed by endoscopic biopsy (including 49 men and 40 women) were collected between June 2004 and March 2006. MSCT was performed one week before operation and all the images were read by two radiologists. The results were compared with the postoperative pathologic findings.</p><p><b>RESULTS</b>In general, the accuracy of MSCT in determining the T stage of gastric carcinoma was 68.7% based on axial and MPR images. The accuracy of diagnosis for T (1) was 90.0% (9/10), for T(2) was 82.8% (24/29), for T(3) was 69.6% (16/23) and for T(4) was 52.4% (11/21) respectively. The accuracy for MSCT in determining the N stage of gastric carcinoma was 51.8% in general. The accuracy of diagnosis for N(0) was 44.0% (11/25), for N(1) was 72.0% (18/25), for N(2) was 46.4% (13/28) and for N(3) was 40.0% (2/5) respectively. The accuracy of diagnosis for distant metastasis (M(1)) was 90% (9/10).</p><p><b>CONCLUSIONS</b>The accuracy of MSCT in preoperative staging of gastric carcinoma is relatively high. It is easily accepted by the patients as a practical and noninvasive technique of gastric carcinoma staging.</p>
Subject(s)
Adult , Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Neoplasm Staging , Methods , Stomach Neoplasms , Diagnostic Imaging , Pathology , Tomography, Spiral ComputedABSTRACT
Objective To diagnose the intrauterine occupational disease by vaginal ultrasonography or hysteroscopy, and to evaluate the diagnostic values of these two methods. Methods One hundred and fifty patients were detected by vaginal ultrasonography and hysteroscopy from Jan 2004 to Dec 2004, and a total of 71 cases were confirmed intrauterine occupational disease. Tissue hysteroscopy or bioposy were performed during the hysterosocopy in order to analyse the tissue pathology. Results In these 71 patients, 47 were no less than 46 years old. Most of them had abnormal uterine bleeding.Thirty-three cases (46.48%) were suggested by vaginal ultrasonography, and 49 (69.01%) by hysteroscopy. Thirty-eight cases (53.52%) were in line with the pathological result.There were significant differences between vaginal ulterasonography and hysteroscopy in diagnosing the intrauterine occupational disease (P