Effects of light intensity on photosynthetic characteristics and seedling growth of Prunella vulgaris / 中国中药杂志

<p><b>OBJECTIVE</b>To study the effect of light intensity on photosynthetic characteristics and seedling growth of Prunella vulgaris under different light intensity.</p><p><b>METHOD</b>The seedling of P. vulgaris was shaded with shading net, and relative parameters were measured. The data was statistically processed.</p><p><b>RESULT</b>With the reducing light intensity, the young plant stems became slender, the leaf long increased. The plant height and net growth under 42% full light condition were significantly higher than those under the other light intensity, chlorophy II and carotenoid contents increased, as well as Chl a/b decreased under low light. Photosynthetic indicators of P(n), T(r) decreased under low light, but CO2 concentration increased, and G(s) content decreases and then increased under 10% full light intensity.</p><p><b>CONCLUSION</b>P. vulgaris manifested the different plasticity and adaptability under different light intensities. The 42% light intensity was the best and the proper shading would promote the growth of seedlings.</p>

Analysis of genetic differentiation and phylogenetic relationship between Whitmania pigra and Hirudo nipponia based on ITS sequence / 中国中药杂志

<p><b>OBJECTIVE</b>To evaluate the genetic differentiation and phylogenetic relationship between Whitmania pigra Whitman and Hirudo nipponia Whitman.</p><p><b>METHOD</b>By the sequences of the rDNA internal transcribed spacer (ITS) the molecular phylogenetic tree was constructed by MP method using software MEGA 4.0.</p><p><b>RESULT</b>The average length of ITS was 857.2-861.2 bp. The A, T, G and C contents in this fragment were 25.12%, 28.28%, 17.34%, 29.29%, respectively. The GC content was higher than the AT content. Little sequence variation was observed in ITS gene fragments with in species, and transition in only 45 loci was revealed in 14 populations. 14 W. pigra and H. nipponia populations were clustered into 2 groups by MP phylogenetic tree.</p><p><b>CONCLUSION</b>The results also showed that the intraspecific variation was dominated in variation types of W. pigra and H. nipponia. The classification result of W. pigra and H. nipponia and its adulterants based on DNA sequences are not totally consistent with those based on classification. It showed that a little of mutation of base in ITS sequences had occurred in the process of evolution, such as transition cites, transvertion cites among base or base gap.</p>

Comparation study on quality of different population of wild and breeding Whitmania pigra / 中国中药杂志

<p><b>OBJECTIVE</b>To examine the quality of Whitmania pigra from the different populations to provide the basis for new species selection.</p><p><b>METHOD</b>The contents of the moisture, alcohol extractive, total ash, acid-insoluble ash, and the activity of antiplatelet aggregation enzyme of wild and the breeding W. pigra were determined by the methods were determined in Chinese Pharmacopoeia (2005 edition). The contents xanthine and hypoxanthine by HPLC.</p><p><b>RESULT</b>It showed that the contents of the alcohol extractive, total ash, acid-insoluble ash, antiplatelet aggregation enzyme, and xanthine and hypoxanthine content of the breeding population in Nanjing are lations. Many quality indexes of the breeding in Zhejiang Tongxiang base were beffer than the wild.</p><p><b>CONCLUSION</b>The breeding W. pigra are good in quality, It suggested large-scale promotion of aquaculture.</p>

Qualities of Whitmania pigra in different-month-age and best harvest time / 中国中药杂志

<p><b>OBJECTIVE</b>Dynamic changes of quality and growth of Whitmania pigra were investigated to provide theoretical basis for quality control and determination of optimal harvest time.</p><p><b>METHOD</b>The contents of moisture, ethanol-soluble extractive, total ash, and acid-insoluble ash, as well as antithrombin activity were determined according to Chinese pharmacopoeia (2005 edition).</p><p><b>RESULT</b>Qualities of W. pigra collected from different growth stages met the standards of Chinese pharmacopoeia (2005 edition). The highest amount of ethanol-soluble extract was found in 9-month-old, W. pigra, followed by 6-month-aged ones. And there was significant difference between 9-month-old and 4-month-old, 7 month-old and 11 month-old (P < 0.05). The highest antithrombin activity was detected in 6 month-old W. pigra, followed by 10 month-old. Significant differences of antithrombin activity were found between 6-month-old and 4 month-old ones, and 5 month-old (P < 0.05), however, there were no significant difference between 6 month-old ones with other samples. Eleven-month-old W. pigra got the most dry weight, and there were significant difference between 11 month-old ones with other samples (P < 0.05).</p><p><b>CONCLUSION</b>November, namely for 6 month old artificial cultivated W. pigra was the best harvest time in Jiangsu and Zhejiang provinces.</p>