ABSTRACT
Objective To investigate the effect of XuanHuSuoSan and its disassembled prescriptions on dissolution of psoralen and isopsoralen. Methods The dissolution of psoralen and isopsoralen in XuanHuSuoSan and its disassembled prescriptions were determined by HPLC, using ES Industries Epic Polar C18 column (250 mm×4.6 mm, 5 μm) with methanol and water (55: 45) as mobile phase at 25 °C with a flow rate of 1.0 ml/min and detection wavelength of 246 nm. Results The dissolution of psoralen and isopsoralen had great changes among XuanHuSuoSan and its disassembled prescriptions, and the analysis of variance and SNK showed that there were significant differences among XuanHuSuoSan and its disassembled prescriptions (P < 0.05). Conclusion Radix Angelicae Sinensis and Radix Achyranthis Bidentatae are helpful to the dissolution of psoralen and isopsoralen from Fructus Psoraleae, while Rhizoma Corydalis can inhibit the dissolution of psoralen and isopsoralen.
ABSTRACT
The present study aimed to establish a pharmacodynamic method using the pySolo software to explore the influence of freeze-dried powders of Shuangxia Decoction (SXD) on the sleep of normal Drosophila melanogaster and the Drosophila melanogaster whose sleep was divested by light. The dose-effect and the time-effect relationships of SXD on sleep were examined. The effect-onset concentration of SXD was 0.25%, the plateau appeared at the concentration of 2.5% and the total sleep time showed a downtrend when the concentration was greater than 2.5%. The sleep time was the longest on the fourth day after SXD was given. The fruit fly sleep deprivation model was repeated by light stimulation at night. The middle dosage group (2.5%) had the best insomnia-curing effect. In conclusion, using the pySolo software, an approach for the pharmacodynamics study was established with Drosophila melanogaster as a model organism to determine the insomnia-curing effects of the traditional Chinese medicine (TCM). Our results demonstrated the reliability of this method. The freeze-dried powders of SXD could effectively improve the sleep quality of Drosophila melanogaster.