ABSTRACT
We report the case of an 11-month-old Jehovah's Witness girl with end-stage liver disease secondary to biliary atresia who underwent a successful living-related liver transplantation. The donor was her mother who is a member of Jehovah's Witness. The use of recombinant human erythropoietin increased hemoglobin concentrations during the perioperative period. Intraoperatively, meticulous surgical hemostasis, avoidance of hypothermia, minimal blood sampling, administration of tranexamic acid, and return of the blood scavenged from the operative field by intraoperative blood salvage enabled the completion of the operation without the use of blood products.
Subject(s)
Female , Humans , Infant , Biliary Atresia , Erythropoietin , Hemostasis, Surgical , Hypothermia , Liver Diseases , Liver Transplantation , Liver , Mothers , Operative Blood Salvage , Perioperative Period , Tissue Donors , Tranexamic AcidABSTRACT
BACKGROUND: The basic treatment of malignant tumors is surgery, radiotherapy, chemotherapy. Even though, the object of these treatments is to kill cancer cells, they have limitations. So, in future studies of treatment of cancer, we should look into increasing human immune response using gene therapy in order to induce damage to tumor cells. OBJECTIVE: The cell growth inhibitory effect of cervical cancer cells was investigated by direct transfection using liposome(pRcCMVp53/lipofectin). and by indirect transfection using Adenovirus(AdCMVp53). METHODS: The cervical cancer cell lines we used in this study were HPV16 positive, having inhibitory gene, wild p53 gene, CaSki, SiHa, HPV18 positive HeLa, HeLaS3 and HPV negative C33A, HT3, LacZ gene was used as the marker gene for the transfection efficacy. Direct transfection was done by using lipofectin (pRcCMVp53/lipofectin) and indirect transfection was done by using virus, AdCMVp53. The effect of tumor cell growth inhibition was measured by cell counting assay. RESULT: Inhibition of growth of cervical cancer cells in cell counts of direct transfection was CaSki(88.5%), SiHa(59.1%), HeLa(86.0%), HeLaS3(78.0%), C33A(91.3%) and HT3(74.0%). Inhibition of growth of cervical cancer cells in cell counts of indirect transfection was CaSki(97.4%), SiHa(91.6%), HeLa(95.8%), HeLaS3(99.7%), C33A(97.3%) and HT3(87.4%). CONCLUSION: The inhibition of cell growth of cervical cancer cells by direct and indirect transfection was significantly reduced, and showed little differences depending on the type of cells. These results will have a great meaning in treating cervical cancer patients using gene therapy by direct or indirect transfection